P09964 (RCRO_BPP22) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Regulatory protein cro

Alternative name(s):
Antirepressor

Gene names

Name:

cro

Organism

Enterobacteria phage P22 (Bacteriophage P22)

Taxonomic identifier

10754 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Podoviridae › P22-like viruses

Virus host

Salmonella typhimurium [TaxID: 90371]

Protein attributes

Sequence length	61 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

Cro represses genes normally expressed in early phage development and is necessary for the late stage of lytic growth. It does this by binding to the OL and OR operators regions normally used by the repressor protein for lysogenic maintenance.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Developmental stage	Early protein
Ligand	DNA-binding
Molecular function	Repressor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 61

61

Regulatory protein cro

PRO_0000077578

Regions

DNA binding

13 – 32

20

H-T-H motif Potential

Secondary structure

1

.

.

.

.

.

.

.

.

.

61

Helix Strand Turn

Details...

Helix

3 – 10

8

Helix

13 – 20

8

Helix

24 – 29

6

Helix

36 – 45

10

Sequences

	Sequence		Length	Mass (Da)
	P09964 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: CD37A130D8CF14AF	FASTA	61	6,828
	10 20 30 40 50 60 MYKKDVIDHF GTQRAVAKAL GISDAAVSQW KEVIPEKDAY RLEIVTAGAL KYQENAYRQA A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bacteriophage P22 Cro protein: sequence, purification, and properties." Poteete A.R., Hehir K., Sauer R.T. Biochemistry 25:251-256(1986) [PubMed: 3954988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence of the genome of Salmonella bacteriophage P22." Vander Byl C.S., Kropinski A.M.B. J. Bacteriol. 182:6472-6481(2000) [PubMed: 11053393] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Corrected sequence of the bacteriophage P22 genome." Pedulla M.L., Ford M.E., Karthikeyan T., Houtz J.M., Hendrix R.W., Hatfull G.F., Poteete A.R., Gilcrease E.B., Winn-Stapley D.A., Casjens S.R. J. Bacteriol. 185:1475-1477(2003) [PubMed: 12562822] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Sequence analysis of a region from the early right operon in phage P22 including the replication genes 18 and 12." Backhaus H., Petri J.B. Gene 32:289-303(1984) [PubMed: 6241581] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 47-61.
[5]	"Secondary structure switching in Cro protein evolution." Newlove T., Konieczka J.H., Cordes M.H. Structure 12:569-581(2004) [PubMed: 15062080] [Abstract] Cited for: STRUCTURE BY NMR.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M12584 Genomic DNA. Translation: AAA32268.1.
AF217253 Genomic DNA. Translation: AAF75025.1.
BK000583 Genomic DNA. Translation: DAA01022.1.
M10074 Genomic DNA. Translation: AAA32273.1.

PIR

RGBP22. A25867.

RefSeq

NP_059607.1. NC_002371.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RZS	NMR	-	A	1-61	[»]

ProteinModelPortal

P09964.

SMR

P09964. Positions 1-61.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1262827.

GenomeReviews

Gene locus cro in contig AF217253_GR.
Gene locus cro in contig BK000583_GR.

Phylogenomic databases

ProtClustDB

CLSP2491675.

Family and domain databases

InterPro

IPR010982. Lambda_DNA-bd.
[Graphical view]

Gene3D

G3DSA:1.10.260.40. G3DSA:1.10.260.40. 1 hit.

SUPFAM

SSF47413. Lambda_like_DNA. 1 hit.

ProtoNet

Search...

Entry information

Entry name

RCRO_BPP22

Accession

Primary (citable) accession number: P09964
Secondary accession number(s): Q38659, Q7PCF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	September 21, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program