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Protein

Endolysin

Gene

19

Organism
Salmonella phage P22 (Bacteriophage P22)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer (By similarity).By similarity

Catalytic activityi

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei16Proton donorBy similarity1
Active sitei25NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase
Biological processCytolysis, Host cell lysis by virus, Viral release from host cell

Protein family/group databases

CAZyiGH24. Glycoside Hydrolase Family 24.

Names & Taxonomyi

Protein namesi
Recommended name:
Endolysin (EC:3.2.1.17)
Alternative name(s):
Lysis protein
Lysozyme
Muramidase
Gene namesi
Name:19
OrganismiSalmonella phage P22 (Bacteriophage P22)
Taxonomic identifieri10754 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeP22virus
Virus hostiSalmonella typhimurium [TaxID: 90371]
Proteomesi
  • UP000001795 Componenti: Genome
  • UP000007960 Componenti: Genome

Subcellular locationi

  • Host cytoplasm Curated
  • Note: The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002181021 – 146EndolysinAdd BLAST146

Expressioni

Keywords - Developmental stagei

Late protein

Structurei

Secondary structure

1146
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 16Combined sources11
Beta strandi19 – 24Combined sources6
Beta strandi30 – 33Combined sources4
Helixi53 – 63Combined sources11
Helixi65 – 74Combined sources10
Helixi81 – 94Combined sources14
Helixi96 – 100Combined sources5
Helixi103 – 109Combined sources7
Helixi113 – 119Combined sources7
Helixi120 – 122Combined sources3
Turni130 – 133Combined sources4
Helixi134 – 145Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ANVX-ray1.04A/B1-146[»]
2ANXX-ray1.04A/B1-146[»]
ProteinModelPortaliP09963.
SMRiP09963.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09963.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi136 – 141Poly-Arg6

Sequence similaritiesi

Belongs to the glycosyl hydrolase 24 family.Curated

Phylogenomic databases

OrthoDBiVOG090000CE.

Family and domain databases

CDDicd00737. endolysin_autolysin. 1 hit.
Gene3Di1.10.530.40. 1 hit.
InterProiView protein in InterPro
IPR033907. Endolysin_autolysin.
IPR002196. Glyco_hydro_24.
IPR023346. Lysozyme-like_dom_sf.
IPR023347. Lysozyme_dom_sf.
PfamiView protein in Pfam
PF00959. Phage_lysozyme. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.

Sequencei

Sequence statusi: Complete.

P09963-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMQISSNGIT RLKREEGERL KAYSDSRGIP TIGVGHTGKV DGNSVASGMT
60 70 80 90 100
ITAEKSSELL KEDLQWVEDA ISSLVRVPLN QNQYDALCSL IFNIGKSAFA
110 120 130 140
GSTVLRQLNL KNYQAAADAF LLWKKAGKDP DILLPRRRRE RALFLS
Length:146
Mass (Da):16,139
Last modified:July 1, 1989 - v1
Checksum:i3A16675AB1A635EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10997 Genomic DNA. Translation: AAA32266.1.
AF217253 Genomic DNA. Translation: AAF75040.1.
BK000583 Genomic DNA. Translation: DAA01040.1.
J04356 Genomic DNA. Translation: AAA88340.1.
PIRiS22904. LZBP22.
RefSeqiNP_059622.1. NC_002371.2.

Genome annotation databases

GeneIDi1262838.
KEGGivg:1262838.

Similar proteinsi

Entry informationi

Entry nameiENLYS_BPP22
AccessioniPrimary (citable) accession number: P09963
Secondary accession number(s): Q7PCD5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 22, 2017
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families