Reviewed, UniProtKB/Swiss-Prot P09963 (LYS_BPP22)

Names and origin

Protein names

Recommended name:
    Lysozyme
    EC=3.2.1.17
Alternative name(s):
    Lysis protein
    Muramidase
    Endolysin

Gene names

Name:

19

Organism

Enterobacteria phage P22 (Bacteriophage P22)

Taxonomic identifier

10754 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Podoviridae › P22-like viruses

Virus host

Salmonella typhimurium [TaxID: 90371]

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Essential for lysis of bacterial cell wall, by showing cell wall hydrolyzing activity.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Sequence similarities	Belongs to the glycosyl hydrolase 24 family.

Ontologies

Keywords
Developmental stage	Late protein
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 146

146

Lysozyme

PRO_0000218102

Sites

Active site

16

1

Proton donor By similarity

Active site

25

1

Nucleophile By similarity

Secondary structure

1

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146

Helix Strand Turn

Details...

Helix

6 – 16

11

Beta strand

19 – 24

6

Beta strand

30 – 33

4

Helix

53 – 63

11

Helix

65 – 74

10

Helix

81 – 94

14

Helix

96 – 100

5

Helix

103 – 109

7

Helix

113 – 119

7

Helix

120 – 122

3

Turn

130 – 133

4

Helix

134 – 145

12

Sequences

	Sequence		Length	Mass (Da)
	P09963-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 3A16675AB1A635EB	FASTA	146	16,139
	10 20 30 40 50 60 MMQISSNGIT RLKREEGERL KAYSDSRGIP TIGVGHTGKV DGNSVASGMT ITAEKSSELL 70 80 90 100 110 120 KEDLQWVEDA ISSLVRVPLN QNQYDALCSL IFNIGKSAFA GSTVLRQLNL KNYQAAADAF 130 140 LLWKKAGKDP DILLPRRRRE RALFLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Phage P22 lysis genes: nucleotide sequences and functional relationships with T4 and lambda genes." Rennell D., Poteete A.R. Virology 143:280-289(1985) [PubMed: 2998005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Sequence of the genome of Salmonella bacteriophage P22." Vander Byl C.S., Kropinski A.M.B. J. Bacteriol. 182:6472-6481(2000) [PubMed: 11053393] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Corrected sequence of the bacteriophage P22 genome." Pedulla M.L., Ford M.E., Karthikeyan T., Houtz J.M., Hendrix R.W., Hatfull G.F., Poteete A.R., Gilcrease E.B., Winn-Stapley D.A., Casjens S.R. J. Bacteriol. 185:1475-1477(2003) [PubMed: 12562822] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Nucleotide sequence of the bacteriophage P22 gene 19 to 3 region: identification of a new gene required for lysis." Casjens S., Eppler K., Parr R., Poteete A.R. Virology 171:588-598(1989) [PubMed: 2763468] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 111-146.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M10997 Genomic DNA. Translation: AAA32266.1.
AF217253 Genomic DNA. Translation: AAF75040.1.
BK000583 Genomic DNA. Translation: DAA01040.1.
J04356 Genomic DNA. Translation: AAA88340.1.

PIR

LZBP22. S22904.

RefSeq

NP_059622.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2ANV	X-ray	1.04	A/B	1-146	[»]
2ANX	X-ray	1.04	A/B	1-146	[»]

ModBase

Search...

Protein family/group databases

CAZy

GH24. Glycoside Hydrolase Family 24.

Genome annotation databases

GeneID

1262838.

GenomeReviews

Gene locus 19 in contig AF217253_GR.
Gene locus 19 in contig BK000583_GR.

Family and domain databases

InterPro

IPR002196. Glyco_hydro_24.
[Graphical view]

Pfam

PF00959. Phage_lysozyme. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

LYS_BPP22

Accession

Primary (citable) accession number: P09963
Secondary accession number(s): Q7PCD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)