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Protein

Endolysin

Gene

19

Organism
Enterobacteria phage P22 (Bacteriophage P22)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer (By similarity).By similarity

Catalytic activityi

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei16Proton donorBy similarity1
Active sitei25NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

Keywords - Biological processi

Cytolysis, Host cell lysis by virus, Virus exit from host cell

Protein family/group databases

CAZyiGH24. Glycoside Hydrolase Family 24.

Names & Taxonomyi

Protein namesi
Recommended name:
Endolysin (EC:3.2.1.17)
Alternative name(s):
Lysis protein
Lysozyme
Muramidase
Gene namesi
Name:19
OrganismiEnterobacteria phage P22 (Bacteriophage P22)
Taxonomic identifieri10754 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeP22likevirus
Virus hostiSalmonella typhimurium [TaxID: 90371]
Proteomesi
  • UP000001795 Componenti: Genome
  • UP000007960 Componenti: Genome

Subcellular locationi

  • Host cytoplasm Curated

  • Note: The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002181021 – 146EndolysinAdd BLAST146

Expressioni

Keywords - Developmental stagei

Late protein

Structurei

Secondary structure

1146
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 16Combined sources11
Beta strandi19 – 24Combined sources6
Beta strandi30 – 33Combined sources4
Helixi53 – 63Combined sources11
Helixi65 – 74Combined sources10
Helixi81 – 94Combined sources14
Helixi96 – 100Combined sources5
Helixi103 – 109Combined sources7
Helixi113 – 119Combined sources7
Helixi120 – 122Combined sources3
Turni130 – 133Combined sources4
Helixi134 – 145Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ANVX-ray1.04A/B1-146[»]
2ANXX-ray1.04A/B1-146[»]
ProteinModelPortaliP09963.
SMRiP09963.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09963.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi136 – 141Poly-Arg6

Sequence similaritiesi

Belongs to the glycosyl hydrolase 24 family.Curated

Phylogenomic databases

KOiK01185.

Family and domain databases

CDDicd00737. endolysin_autolysin. 1 hit.
Gene3Di1.10.530.40. 1 hit.
InterProiIPR033907. Endolysin_autolysin.
IPR002196. Glyco_hydro_24.
IPR023346. Lysozyme-like_dom.
IPR023347. Lysozyme_dom.
[Graphical view]
PfamiPF00959. Phage_lysozyme. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.

Sequencei

Sequence statusi: Complete.

P09963-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMQISSNGIT RLKREEGERL KAYSDSRGIP TIGVGHTGKV DGNSVASGMT
60 70 80 90 100
ITAEKSSELL KEDLQWVEDA ISSLVRVPLN QNQYDALCSL IFNIGKSAFA
110 120 130 140
GSTVLRQLNL KNYQAAADAF LLWKKAGKDP DILLPRRRRE RALFLS
Length:146
Mass (Da):16,139
Last modified:July 1, 1989 - v1
Checksum:i3A16675AB1A635EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10997 Genomic DNA. Translation: AAA32266.1.
AF217253 Genomic DNA. Translation: AAF75040.1.
BK000583 Genomic DNA. Translation: DAA01040.1.
J04356 Genomic DNA. Translation: AAA88340.1.
PIRiS22904. LZBP22.
RefSeqiNP_059622.1. NC_002371.2.

Genome annotation databases

GeneIDi1262838.
KEGGivg:1262838.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10997 Genomic DNA. Translation: AAA32266.1.
AF217253 Genomic DNA. Translation: AAF75040.1.
BK000583 Genomic DNA. Translation: DAA01040.1.
J04356 Genomic DNA. Translation: AAA88340.1.
PIRiS22904. LZBP22.
RefSeqiNP_059622.1. NC_002371.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ANVX-ray1.04A/B1-146[»]
2ANXX-ray1.04A/B1-146[»]
ProteinModelPortaliP09963.
SMRiP09963.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH24. Glycoside Hydrolase Family 24.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1262838.
KEGGivg:1262838.

Phylogenomic databases

KOiK01185.

Miscellaneous databases

EvolutionaryTraceiP09963.

Family and domain databases

CDDicd00737. endolysin_autolysin. 1 hit.
Gene3Di1.10.530.40. 1 hit.
InterProiIPR033907. Endolysin_autolysin.
IPR002196. Glyco_hydro_24.
IPR023346. Lysozyme-like_dom.
IPR023347. Lysozyme_dom.
[Graphical view]
PfamiPF00959. Phage_lysozyme. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENLYS_BPP22
AccessioniPrimary (citable) accession number: P09963
Secondary accession number(s): Q7PCD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 30, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.