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P09963

- ENLYS_BPP22

UniProt

P09963 - ENLYS_BPP22

Protein

Endolysin

Gene

19

Organism
Enterobacteria phage P22 (Bacteriophage P22)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer By similarity.By similarity

    Catalytic activityi

    Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei16 – 161Proton donorBy similarity
    Active sitei25 – 251NucleophileBy similarity

    GO - Molecular functioni

    1. lysozyme activity Source: UniProtKB-EC

    GO - Biological processi

    1. cell wall macromolecule catabolic process Source: InterPro
    2. cytolysis Source: UniProtKB-KW
    3. defense response to bacterium Source: UniProtKB-KW
    4. peptidoglycan catabolic process Source: InterPro
    5. viral release from host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

    Keywords - Biological processi

    Cytolysis, Host cell lysis by virus, Virus exit from host cell

    Protein family/group databases

    CAZyiGH24. Glycoside Hydrolase Family 24.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endolysin (EC:3.2.1.17)
    Alternative name(s):
    Lysis protein
    Lysozyme
    Muramidase
    Gene namesi
    Name:19
    OrganismiEnterobacteria phage P22 (Bacteriophage P22)
    Taxonomic identifieri10754 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeP22likevirus
    Virus hostiSalmonella typhimurium [TaxID: 90371]
    ProteomesiUP000001795: Genome, UP000007960: Genome

    Subcellular locationi

    Host cytoplasm Curated
    Note: The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane.By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 146146EndolysinPRO_0000218102Add
    BLAST

    Expressioni

    Keywords - Developmental stagei

    Late protein

    Structurei

    Secondary structure

    1
    146
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 1611
    Beta strandi19 – 246
    Beta strandi30 – 334
    Helixi53 – 6311
    Helixi65 – 7410
    Helixi81 – 9414
    Helixi96 – 1005
    Helixi103 – 1097
    Helixi113 – 1197
    Helixi120 – 1223
    Turni130 – 1334
    Helixi134 – 14512

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2ANVX-ray1.04A/B1-146[»]
    2ANXX-ray1.04A/B1-146[»]
    ProteinModelPortaliP09963.
    SMRiP09963. Positions 1-146.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP09963.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi136 – 1416Poly-Arg

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 24 family.Curated

    Family and domain databases

    Gene3Di1.10.530.40. 1 hit.
    InterProiIPR002196. Glyco_hydro_24.
    IPR023346. Lysozyme-like_dom.
    IPR023347. Lysozyme_dom.
    [Graphical view]
    PfamiPF00959. Phage_lysozyme. 1 hit.
    [Graphical view]
    SUPFAMiSSF53955. SSF53955. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P09963-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMQISSNGIT RLKREEGERL KAYSDSRGIP TIGVGHTGKV DGNSVASGMT    50
    ITAEKSSELL KEDLQWVEDA ISSLVRVPLN QNQYDALCSL IFNIGKSAFA 100
    GSTVLRQLNL KNYQAAADAF LLWKKAGKDP DILLPRRRRE RALFLS 146
    Length:146
    Mass (Da):16,139
    Last modified:July 1, 1989 - v1
    Checksum:i3A16675AB1A635EB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M10997 Genomic DNA. Translation: AAA32266.1.
    AF217253 Genomic DNA. Translation: AAF75040.1.
    BK000583 Genomic DNA. Translation: DAA01040.1.
    J04356 Genomic DNA. Translation: AAA88340.1.
    PIRiS22904. LZBP22.
    RefSeqiNP_059622.1. NC_002371.2.

    Genome annotation databases

    GeneIDi1262838.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M10997 Genomic DNA. Translation: AAA32266.1 .
    AF217253 Genomic DNA. Translation: AAF75040.1 .
    BK000583 Genomic DNA. Translation: DAA01040.1 .
    J04356 Genomic DNA. Translation: AAA88340.1 .
    PIRi S22904. LZBP22.
    RefSeqi NP_059622.1. NC_002371.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2ANV X-ray 1.04 A/B 1-146 [» ]
    2ANX X-ray 1.04 A/B 1-146 [» ]
    ProteinModelPortali P09963.
    SMRi P09963. Positions 1-146.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH24. Glycoside Hydrolase Family 24.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1262838.

    Miscellaneous databases

    EvolutionaryTracei P09963.

    Family and domain databases

    Gene3Di 1.10.530.40. 1 hit.
    InterProi IPR002196. Glyco_hydro_24.
    IPR023346. Lysozyme-like_dom.
    IPR023347. Lysozyme_dom.
    [Graphical view ]
    Pfami PF00959. Phage_lysozyme. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53955. SSF53955. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Phage P22 lysis genes: nucleotide sequences and functional relationships with T4 and lambda genes."
      Rennell D., Poteete A.R.
      Virology 143:280-289(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    2. "Sequence of the genome of Salmonella bacteriophage P22."
      Vander Byl C.S., Kropinski A.M.B.
      J. Bacteriol. 182:6472-6481(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Nucleotide sequence of the bacteriophage P22 gene 19 to 3 region: identification of a new gene required for lysis."
      Casjens S., Eppler K., Parr R., Poteete A.R.
      Virology 171:588-598(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 111-146.
    5. "Extension to 2268 atoms of direct methods in the ab initio determination of the unknown structure of bacteriophage P22 lysozyme."
      Mooers B.H., Matthews B.W.
      Acta Crystallogr. D 62:165-176(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.04 ANGSTROMS).

    Entry informationi

    Entry nameiENLYS_BPP22
    AccessioniPrimary (citable) accession number: P09963
    Secondary accession number(s): Q7PCD5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3