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Protein

Endolysin

Gene

19

Organism
Enterobacteria phage P22 (Bacteriophage P22)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer (By similarity).By similarity

Catalytic activityi

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei16 – 161Proton donorBy similarity
Active sitei25 – 251NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

Keywords - Biological processi

Cytolysis, Host cell lysis by virus, Virus exit from host cell

Protein family/group databases

CAZyiGH24. Glycoside Hydrolase Family 24.

Names & Taxonomyi

Protein namesi
Recommended name:
Endolysin (EC:3.2.1.17)
Alternative name(s):
Lysis protein
Lysozyme
Muramidase
Gene namesi
Name:19
OrganismiEnterobacteria phage P22 (Bacteriophage P22)
Taxonomic identifieri10754 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeP22likevirus
Virus hostiSalmonella typhimurium [TaxID: 90371]
ProteomesiUP000001795 Componenti: Genome UP000007960 Componenti: Genome

Subcellular locationi

  • Host cytoplasm Curated

  • Note: The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 146146EndolysinPRO_0000218102Add
BLAST

Expressioni

Keywords - Developmental stagei

Late protein

Structurei

Secondary structure

1
146
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 1611Combined sources
Beta strandi19 – 246Combined sources
Beta strandi30 – 334Combined sources
Helixi53 – 6311Combined sources
Helixi65 – 7410Combined sources
Helixi81 – 9414Combined sources
Helixi96 – 1005Combined sources
Helixi103 – 1097Combined sources
Helixi113 – 1197Combined sources
Helixi120 – 1223Combined sources
Turni130 – 1334Combined sources
Helixi134 – 14512Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ANVX-ray1.04A/B1-146[»]
2ANXX-ray1.04A/B1-146[»]
ProteinModelPortaliP09963.
SMRiP09963. Positions 1-146.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09963.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi136 – 1416Poly-Arg

Sequence similaritiesi

Belongs to the glycosyl hydrolase 24 family.Curated

Family and domain databases

Gene3Di1.10.530.40. 1 hit.
InterProiIPR002196. Glyco_hydro_24.
IPR023346. Lysozyme-like_dom.
IPR023347. Lysozyme_dom.
[Graphical view]
PfamiPF00959. Phage_lysozyme. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.

Sequencei

Sequence statusi: Complete.

P09963-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMQISSNGIT RLKREEGERL KAYSDSRGIP TIGVGHTGKV DGNSVASGMT
60 70 80 90 100
ITAEKSSELL KEDLQWVEDA ISSLVRVPLN QNQYDALCSL IFNIGKSAFA
110 120 130 140
GSTVLRQLNL KNYQAAADAF LLWKKAGKDP DILLPRRRRE RALFLS
Length:146
Mass (Da):16,139
Last modified:July 1, 1989 - v1
Checksum:i3A16675AB1A635EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10997 Genomic DNA. Translation: AAA32266.1.
AF217253 Genomic DNA. Translation: AAF75040.1.
BK000583 Genomic DNA. Translation: DAA01040.1.
J04356 Genomic DNA. Translation: AAA88340.1.
PIRiS22904. LZBP22.
RefSeqiNP_059622.1. NC_002371.2.

Genome annotation databases

GeneIDi1262838.
KEGGivg:1262838.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10997 Genomic DNA. Translation: AAA32266.1.
AF217253 Genomic DNA. Translation: AAF75040.1.
BK000583 Genomic DNA. Translation: DAA01040.1.
J04356 Genomic DNA. Translation: AAA88340.1.
PIRiS22904. LZBP22.
RefSeqiNP_059622.1. NC_002371.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ANVX-ray1.04A/B1-146[»]
2ANXX-ray1.04A/B1-146[»]
ProteinModelPortaliP09963.
SMRiP09963. Positions 1-146.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH24. Glycoside Hydrolase Family 24.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1262838.
KEGGivg:1262838.

Miscellaneous databases

EvolutionaryTraceiP09963.

Family and domain databases

Gene3Di1.10.530.40. 1 hit.
InterProiIPR002196. Glyco_hydro_24.
IPR023346. Lysozyme-like_dom.
IPR023347. Lysozyme_dom.
[Graphical view]
PfamiPF00959. Phage_lysozyme. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Phage P22 lysis genes: nucleotide sequences and functional relationships with T4 and lambda genes."
    Rennell D., Poteete A.R.
    Virology 143:280-289(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  2. "Sequence of the genome of Salmonella bacteriophage P22."
    Vander Byl C.S., Kropinski A.M.B.
    J. Bacteriol. 182:6472-6481(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Nucleotide sequence of the bacteriophage P22 gene 19 to 3 region: identification of a new gene required for lysis."
    Casjens S., Eppler K., Parr R., Poteete A.R.
    Virology 171:588-598(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 111-146.
  5. "Extension to 2268 atoms of direct methods in the ab initio determination of the unknown structure of bacteriophage P22 lysozyme."
    Mooers B.H., Matthews B.W.
    Acta Crystallogr. D 62:165-176(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.04 ANGSTROMS).

Entry informationi

Entry nameiENLYS_BPP22
AccessioniPrimary (citable) accession number: P09963
Secondary accession number(s): Q7PCD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 1, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.