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P09963

- ENLYS_BPP22

UniProt

P09963 - ENLYS_BPP22

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Protein

Endolysin

Gene

19

Organism
Enterobacteria phage P22 (Bacteriophage P22)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer By similarity.By similarity

Catalytic activityi

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei16 – 161Proton donorBy similarity
Active sitei25 – 251NucleophileBy similarity

GO - Molecular functioni

  1. lysozyme activity Source: UniProtKB-EC

GO - Biological processi

  1. cell wall macromolecule catabolic process Source: InterPro
  2. cytolysis Source: UniProtKB-KW
  3. defense response to bacterium Source: UniProtKB-KW
  4. peptidoglycan catabolic process Source: InterPro
  5. viral release from host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

Keywords - Biological processi

Cytolysis, Host cell lysis by virus, Virus exit from host cell

Protein family/group databases

CAZyiGH24. Glycoside Hydrolase Family 24.

Names & Taxonomyi

Protein namesi
Recommended name:
Endolysin (EC:3.2.1.17)
Alternative name(s):
Lysis protein
Lysozyme
Muramidase
Gene namesi
Name:19
OrganismiEnterobacteria phage P22 (Bacteriophage P22)
Taxonomic identifieri10754 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeP22likevirus
Virus hostiSalmonella typhimurium [TaxID: 90371]
ProteomesiUP000001795: Genome, UP000007960: Genome

Subcellular locationi

Host cytoplasm Curated
Note: The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane.By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 146146EndolysinPRO_0000218102Add
BLAST

Expressioni

Keywords - Developmental stagei

Late protein

Structurei

Secondary structure

1
146
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 1611
Beta strandi19 – 246
Beta strandi30 – 334
Helixi53 – 6311
Helixi65 – 7410
Helixi81 – 9414
Helixi96 – 1005
Helixi103 – 1097
Helixi113 – 1197
Helixi120 – 1223
Turni130 – 1334
Helixi134 – 14512

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ANVX-ray1.04A/B1-146[»]
2ANXX-ray1.04A/B1-146[»]
ProteinModelPortaliP09963.
SMRiP09963. Positions 1-146.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09963.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi136 – 1416Poly-Arg

Sequence similaritiesi

Belongs to the glycosyl hydrolase 24 family.Curated

Family and domain databases

Gene3Di1.10.530.40. 1 hit.
InterProiIPR002196. Glyco_hydro_24.
IPR023346. Lysozyme-like_dom.
IPR023347. Lysozyme_dom.
[Graphical view]
PfamiPF00959. Phage_lysozyme. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.

Sequencei

Sequence statusi: Complete.

P09963-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMQISSNGIT RLKREEGERL KAYSDSRGIP TIGVGHTGKV DGNSVASGMT
60 70 80 90 100
ITAEKSSELL KEDLQWVEDA ISSLVRVPLN QNQYDALCSL IFNIGKSAFA
110 120 130 140
GSTVLRQLNL KNYQAAADAF LLWKKAGKDP DILLPRRRRE RALFLS
Length:146
Mass (Da):16,139
Last modified:July 1, 1989 - v1
Checksum:i3A16675AB1A635EB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M10997 Genomic DNA. Translation: AAA32266.1.
AF217253 Genomic DNA. Translation: AAF75040.1.
BK000583 Genomic DNA. Translation: DAA01040.1.
J04356 Genomic DNA. Translation: AAA88340.1.
PIRiS22904. LZBP22.
RefSeqiNP_059622.1. NC_002371.2.

Genome annotation databases

GeneIDi1262838.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M10997 Genomic DNA. Translation: AAA32266.1 .
AF217253 Genomic DNA. Translation: AAF75040.1 .
BK000583 Genomic DNA. Translation: DAA01040.1 .
J04356 Genomic DNA. Translation: AAA88340.1 .
PIRi S22904. LZBP22.
RefSeqi NP_059622.1. NC_002371.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2ANV X-ray 1.04 A/B 1-146 [» ]
2ANX X-ray 1.04 A/B 1-146 [» ]
ProteinModelPortali P09963.
SMRi P09963. Positions 1-146.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH24. Glycoside Hydrolase Family 24.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1262838.

Miscellaneous databases

EvolutionaryTracei P09963.

Family and domain databases

Gene3Di 1.10.530.40. 1 hit.
InterProi IPR002196. Glyco_hydro_24.
IPR023346. Lysozyme-like_dom.
IPR023347. Lysozyme_dom.
[Graphical view ]
Pfami PF00959. Phage_lysozyme. 1 hit.
[Graphical view ]
SUPFAMi SSF53955. SSF53955. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Phage P22 lysis genes: nucleotide sequences and functional relationships with T4 and lambda genes."
    Rennell D., Poteete A.R.
    Virology 143:280-289(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  2. "Sequence of the genome of Salmonella bacteriophage P22."
    Vander Byl C.S., Kropinski A.M.B.
    J. Bacteriol. 182:6472-6481(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Nucleotide sequence of the bacteriophage P22 gene 19 to 3 region: identification of a new gene required for lysis."
    Casjens S., Eppler K., Parr R., Poteete A.R.
    Virology 171:588-598(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 111-146.
  5. "Extension to 2268 atoms of direct methods in the ab initio determination of the unknown structure of bacteriophage P22 lysozyme."
    Mooers B.H., Matthews B.W.
    Acta Crystallogr. D 62:165-176(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.04 ANGSTROMS).

Entry informationi

Entry nameiENLYS_BPP22
AccessioniPrimary (citable) accession number: P09963
Secondary accession number(s): Q7PCD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 29, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3