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P09961

- AMY1_DICT6

UniProt

P09961 - AMY1_DICT6

Protein

Alpha-amylase 1

Gene

amyA

Organism
Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    This amylase is a highly liquefying-type: oligomers appeared at the beginning of incubation, followed by a graded decrease in the amounts of maltotriose, maltose and glucose in prolonged incubation.

    Catalytic activityi

    Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

    pH dependencei

    Optimum pH is 5.5. Stable above pH 5.5.

    Temperature dependencei

    Optimum temperature is about 90 degrees Celsius. Highly thermostable. Retains 70% of its maximal activity after heating 1 hour at 90 degrees Celsius, but no decrease in activity was observed within the same time at 80 degrees Celsius.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei125 – 1251NucleophileBy similarity
    Active sitei216 – 2161Proton donorBy similarity

    GO - Molecular functioni

    1. alpha-amylase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism

    Enzyme and pathway databases

    BioCyciDTHE309799:GHF9-738-MONOMER.

    Protein family/group databases

    CAZyiGH57. Glycoside Hydrolase Family 57.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-amylase 1 (EC:3.2.1.1)
    Alternative name(s):
    1,4-alpha-D-glucan glucanohydrolase
    Gene namesi
    Name:amyA
    Ordered Locus Names:DICTH_0745
    OrganismiDictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12)
    Taxonomic identifieri309799 [NCBI]
    Taxonomic lineageiBacteriaDictyoglomiDictyoglomalesDictyoglomaceaeDictyoglomus
    ProteomesiUP000001733: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 686685Alpha-amylase 1PRO_0000184571Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi309799.DICTH_0745.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 57 family.Curated

    Phylogenomic databases

    eggNOGiCOG1449.
    HOGENOMiHOG000223207.
    KOiK07405.
    OMAiCNCPYWH.
    OrthoDBiEOG6KT2JZ.

    Family and domain databases

    Gene3Di2.70.98.10. 1 hit.
    3.20.110.10. 1 hit.
    InterProiIPR015179. A-amylase/a-glucTrfase_C.
    IPR015178. A-amylase/a-glucTrfase_central.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR027291. Glyco_hydro_38/57_N.
    IPR028995. Glyco_hydro_57/38_cen.
    IPR004300. Glyco_hydro_57_N.
    [Graphical view]
    PfamiPF09094. DUF1925. 1 hit.
    PF09095. DUF1926. 1 hit.
    PF03065. Glyco_hydro_57. 1 hit.
    [Graphical view]
    ProDomiPD014405. DUF1925. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF74650. SSF74650. 1 hit.
    SSF88688. SSF88688. 1 hit.
    SSF88713. SSF88713. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09961-1 [UniParc]FASTAAdd to Basket

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    MTKSIYFSLG IHNHQPVGNF DFVIERAYEM SYKPLINFFF KHPDFPINVH    50
    FSGFLLLWLE KNHPEYFEKL KIMAERGQIE FVSGGFYEPI LPIIPDKDKV 100
    QQIKKLNKYI YDKFGQTPKG MWLAERVWEP HLVKYIAEAG IEYVVVDDAH 150
    FFSVGLKEED LFGYYLMEEQ GYKLAVFPIS MKLRYLIPFA DPEETITYLD 200
    KFASEDKSKI ALLFDDGEKF GLWPDTYRTV YEEGWLETFV SKIKENFLLV 250
    TPVNLYTYMQ RVKPKGRIYL PTASYREMME WVLFPEAQKE LEELVEKLKT 300
    ENLWDKFSPY VKGGFWRNFL AKYDESNHMQ KKMLYVWKKV QDSPNEEVKE 350
    KAMEEVFQGQ ANDAYWHGIF GGLYLPHLRT AIYEHLIKAE NYLENSEIRF 400
    NIFDFDCDGN DEIIVESPFF NLYLSPNHGG SVLEWDFKTK AFNLTNVLTR 450
    RKEAYHSKLS YVTSEAQGKS IHERWTAKEE GLENILFYDN HRRVSFTEKI 500
    FESEPVLEDL WKDSSRLEVD SFYENYDYEI NKDENKIRVL FSGVFRGFEL 550
    CKSYILYKDK SFVDVVYEIK NVSETPISLN FGWEINLNFL APNHPDYYFL 600
    IGDQKYPLSS FGIEKVNNWK IFSGIGIELE CVLDVEASLY RYPIETVSLS 650
    EEGFERVYQG SALIHFYKVD LPVGSTWRTT IRFWVK 686
    Length:686
    Mass (Da):81,192
    Last modified:January 23, 2007 - v2
    Checksum:iDFF73F94A439E187
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07896 Genomic DNA. Translation: CAA30735.1.
    CP001146 Genomic DNA. Translation: ACI18637.1.
    PIRiS00628. ALDYAT.
    RefSeqiWP_012547269.1. NC_011297.1.
    YP_002250612.1. NC_011297.1.

    Genome annotation databases

    EnsemblBacteriaiACI18637; ACI18637; DICTH_0745.
    GeneIDi6945629.
    KEGGidth:DICTH_0745.
    PATRICi21806130. VBIDicThe33784_0734.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07896 Genomic DNA. Translation: CAA30735.1 .
    CP001146 Genomic DNA. Translation: ACI18637.1 .
    PIRi S00628. ALDYAT.
    RefSeqi WP_012547269.1. NC_011297.1.
    YP_002250612.1. NC_011297.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 309799.DICTH_0745.

    Protein family/group databases

    CAZyi GH57. Glycoside Hydrolase Family 57.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACI18637 ; ACI18637 ; DICTH_0745 .
    GeneIDi 6945629.
    KEGGi dth:DICTH_0745.
    PATRICi 21806130. VBIDicThe33784_0734.

    Phylogenomic databases

    eggNOGi COG1449.
    HOGENOMi HOG000223207.
    KOi K07405.
    OMAi CNCPYWH.
    OrthoDBi EOG6KT2JZ.

    Enzyme and pathway databases

    BioCyci DTHE309799:GHF9-738-MONOMER.

    Family and domain databases

    Gene3Di 2.70.98.10. 1 hit.
    3.20.110.10. 1 hit.
    InterProi IPR015179. A-amylase/a-glucTrfase_C.
    IPR015178. A-amylase/a-glucTrfase_central.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR027291. Glyco_hydro_38/57_N.
    IPR028995. Glyco_hydro_57/38_cen.
    IPR004300. Glyco_hydro_57_N.
    [Graphical view ]
    Pfami PF09094. DUF1925. 1 hit.
    PF09095. DUF1926. 1 hit.
    PF03065. Glyco_hydro_57. 1 hit.
    [Graphical view ]
    ProDomi PD014405. DUF1925. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF74650. SSF74650. 1 hit.
    SSF88688. SSF88688. 1 hit.
    SSF88713. SSF88713. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and nucleotide sequence of a heat-stable amylase gene from an anaerobic thermophile, Dictyoglomus thermophilum."
      Fukusumi S., Kamizono A., Horinouchi S., Beppu T.
      Eur. J. Biochem. 174:15-21(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-13.
    2. "The complete genome sequence of Dictyoglomus thermophilum strain ATCC 35947 / DSM 3960 / H-6-12."
      Dodson R.J., Durkin A.S., Wu M., Eisen J., Sutton G.
      Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35947 / DSM 3960 / H-6-12.

    Entry informationi

    Entry nameiAMY1_DICT6
    AccessioniPrimary (citable) accession number: P09961
    Secondary accession number(s): B5YDK4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 87 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3