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Protein

Alpha-amylase 1

Gene

amyA

Organism
Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This amylase is a highly liquefying-type: oligomers appeared at the beginning of incubation, followed by a graded decrease in the amounts of maltotriose, maltose and glucose in prolonged incubation.

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

pH dependencei

Optimum pH is 5.5. Stable above pH 5.5.

Temperature dependencei

Optimum temperature is about 90 degrees Celsius. Highly thermostable. Retains 70% of its maximal activity after heating 1 hour at 90 degrees Celsius, but no decrease in activity was observed within the same time at 80 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei125 – 1251NucleophileBy similarity
Active sitei216 – 2161Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BioCyciDTHE309799:GHF9-738-MONOMER.

Protein family/group databases

CAZyiGH57. Glycoside Hydrolase Family 57.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase 1 (EC:3.2.1.1)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:amyA
Ordered Locus Names:DICTH_0745
OrganismiDictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12)
Taxonomic identifieri309799 [NCBI]
Taxonomic lineageiBacteriaDictyoglomiDictyoglomalesDictyoglomaceaeDictyoglomus
ProteomesiUP000001733 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 686685Alpha-amylase 1PRO_0000184571Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi309799.DICTH_0745.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 57 family.Curated

Phylogenomic databases

eggNOGiCOG1449.
HOGENOMiHOG000223207.
KOiK07405.
OMAiHFMSAGL.
OrthoDBiEOG6KT2JZ.

Family and domain databases

Gene3Di2.70.98.10. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR015179. A-amylase/a-glucTrfase_C.
IPR015178. A-amylase/a-glucTrfase_central.
IPR011013. Gal_mutarotase_SF_dom.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR027291. Glyco_hydro_38/57_N.
IPR028995. Glyco_hydro_57/38_cen.
IPR004300. Glyco_hydro_57_N.
[Graphical view]
PfamiPF09094. DUF1925. 1 hit.
PF09095. DUF1926. 1 hit.
PF03065. Glyco_hydro_57. 1 hit.
[Graphical view]
ProDomiPD014405. DUF1925. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09961-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKSIYFSLG IHNHQPVGNF DFVIERAYEM SYKPLINFFF KHPDFPINVH
60 70 80 90 100
FSGFLLLWLE KNHPEYFEKL KIMAERGQIE FVSGGFYEPI LPIIPDKDKV
110 120 130 140 150
QQIKKLNKYI YDKFGQTPKG MWLAERVWEP HLVKYIAEAG IEYVVVDDAH
160 170 180 190 200
FFSVGLKEED LFGYYLMEEQ GYKLAVFPIS MKLRYLIPFA DPEETITYLD
210 220 230 240 250
KFASEDKSKI ALLFDDGEKF GLWPDTYRTV YEEGWLETFV SKIKENFLLV
260 270 280 290 300
TPVNLYTYMQ RVKPKGRIYL PTASYREMME WVLFPEAQKE LEELVEKLKT
310 320 330 340 350
ENLWDKFSPY VKGGFWRNFL AKYDESNHMQ KKMLYVWKKV QDSPNEEVKE
360 370 380 390 400
KAMEEVFQGQ ANDAYWHGIF GGLYLPHLRT AIYEHLIKAE NYLENSEIRF
410 420 430 440 450
NIFDFDCDGN DEIIVESPFF NLYLSPNHGG SVLEWDFKTK AFNLTNVLTR
460 470 480 490 500
RKEAYHSKLS YVTSEAQGKS IHERWTAKEE GLENILFYDN HRRVSFTEKI
510 520 530 540 550
FESEPVLEDL WKDSSRLEVD SFYENYDYEI NKDENKIRVL FSGVFRGFEL
560 570 580 590 600
CKSYILYKDK SFVDVVYEIK NVSETPISLN FGWEINLNFL APNHPDYYFL
610 620 630 640 650
IGDQKYPLSS FGIEKVNNWK IFSGIGIELE CVLDVEASLY RYPIETVSLS
660 670 680
EEGFERVYQG SALIHFYKVD LPVGSTWRTT IRFWVK
Length:686
Mass (Da):81,192
Last modified:January 23, 2007 - v2
Checksum:iDFF73F94A439E187
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07896 Genomic DNA. Translation: CAA30735.1.
CP001146 Genomic DNA. Translation: ACI18637.1.
PIRiS00628. ALDYAT.
RefSeqiWP_012547269.1. NC_011297.1.
YP_002250612.1. NC_011297.1.

Genome annotation databases

EnsemblBacteriaiACI18637; ACI18637; DICTH_0745.
KEGGidth:DICTH_0745.
PATRICi21806130. VBIDicThe33784_0734.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07896 Genomic DNA. Translation: CAA30735.1.
CP001146 Genomic DNA. Translation: ACI18637.1.
PIRiS00628. ALDYAT.
RefSeqiWP_012547269.1. NC_011297.1.
YP_002250612.1. NC_011297.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi309799.DICTH_0745.

Protein family/group databases

CAZyiGH57. Glycoside Hydrolase Family 57.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACI18637; ACI18637; DICTH_0745.
KEGGidth:DICTH_0745.
PATRICi21806130. VBIDicThe33784_0734.

Phylogenomic databases

eggNOGiCOG1449.
HOGENOMiHOG000223207.
KOiK07405.
OMAiHFMSAGL.
OrthoDBiEOG6KT2JZ.

Enzyme and pathway databases

BioCyciDTHE309799:GHF9-738-MONOMER.

Family and domain databases

Gene3Di2.70.98.10. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR015179. A-amylase/a-glucTrfase_C.
IPR015178. A-amylase/a-glucTrfase_central.
IPR011013. Gal_mutarotase_SF_dom.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR027291. Glyco_hydro_38/57_N.
IPR028995. Glyco_hydro_57/38_cen.
IPR004300. Glyco_hydro_57_N.
[Graphical view]
PfamiPF09094. DUF1925. 1 hit.
PF09095. DUF1926. 1 hit.
PF03065. Glyco_hydro_57. 1 hit.
[Graphical view]
ProDomiPD014405. DUF1925. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and nucleotide sequence of a heat-stable amylase gene from an anaerobic thermophile, Dictyoglomus thermophilum."
    Fukusumi S., Kamizono A., Horinouchi S., Beppu T.
    Eur. J. Biochem. 174:15-21(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-13.
  2. "The complete genome sequence of Dictyoglomus thermophilum strain ATCC 35947 / DSM 3960 / H-6-12."
    Dodson R.J., Durkin A.S., Wu M., Eisen J., Sutton G.
    Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35947 / DSM 3960 / H-6-12.

Entry informationi

Entry nameiAMY1_DICT6
AccessioniPrimary (citable) accession number: P09961
Secondary accession number(s): B5YDK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: April 1, 2015
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.