P09961 (AMY1_DICT6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-amylase 1
EC=3.2.1.1

Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase

Gene names

Name:	amyA
Ordered Locus Names:	DICTH_0745

Organism

Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12) [Complete proteome] [HAMAP]

Taxonomic identifier

309799 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Dictyoglomi › Dictyoglomia › Dictyoglomales › Dictyoglomaceae › Dictyoglomus › Dictyoglomus thermophilum

Protein attributes

Sequence length	686 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This amylase is a highly liquefying-type: oligomers appeared at the beginning of incubation, followed by a graded decrease in the amounts of maltotriose, maltose and glucose in prolonged incubation.
Catalytic activity	Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glycosyl hydrolase 57 family.
Biophysicochemical properties	pH dependence: Optimum pH is 5.5. Stable above pH 5.5. Temperature dependence: Optimum temperature is about 90 degrees Celsius. Highly thermostable. Retains 70% of its maximal activity after heating 1 hour at 90 degrees Celsius, but no decrease in activity was observed within the same time at 80 degrees Celsius.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	alpha-amylase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 686	685	Alpha-amylase 1		PRO_0000184571
Sites
	Active site	125	1	Nucleophile By similarity
	Active site	216	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P09961 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: DFF73F94A439E187	FASTA	686	81,192
	10 20 30 40 50 60 MTKSIYFSLG IHNHQPVGNF DFVIERAYEM SYKPLINFFF KHPDFPINVH FSGFLLLWLE 70 80 90 100 110 120 KNHPEYFEKL KIMAERGQIE FVSGGFYEPI LPIIPDKDKV QQIKKLNKYI YDKFGQTPKG 130 140 150 160 170 180 MWLAERVWEP HLVKYIAEAG IEYVVVDDAH FFSVGLKEED LFGYYLMEEQ GYKLAVFPIS 190 200 210 220 230 240 MKLRYLIPFA DPEETITYLD KFASEDKSKI ALLFDDGEKF GLWPDTYRTV YEEGWLETFV 250 260 270 280 290 300 SKIKENFLLV TPVNLYTYMQ RVKPKGRIYL PTASYREMME WVLFPEAQKE LEELVEKLKT 310 320 330 340 350 360 ENLWDKFSPY VKGGFWRNFL AKYDESNHMQ KKMLYVWKKV QDSPNEEVKE KAMEEVFQGQ 370 380 390 400 410 420 ANDAYWHGIF GGLYLPHLRT AIYEHLIKAE NYLENSEIRF NIFDFDCDGN DEIIVESPFF 430 440 450 460 470 480 NLYLSPNHGG SVLEWDFKTK AFNLTNVLTR RKEAYHSKLS YVTSEAQGKS IHERWTAKEE 490 500 510 520 530 540 GLENILFYDN HRRVSFTEKI FESEPVLEDL WKDSSRLEVD SFYENYDYEI NKDENKIRVL 550 560 570 580 590 600 FSGVFRGFEL CKSYILYKDK SFVDVVYEIK NVSETPISLN FGWEINLNFL APNHPDYYFL 610 620 630 640 650 660 IGDQKYPLSS FGIEKVNNWK IFSGIGIELE CVLDVEASLY RYPIETVSLS EEGFERVYQG 670 680 SALIHFYKVD LPVGSTWRTT IRFWVK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and nucleotide sequence of a heat-stable amylase gene from an anaerobic thermophile, Dictyoglomus thermophilum." Fukusumi S., Kamizono A., Horinouchi S., Beppu T. Eur. J. Biochem. 174:15-21(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-13.
[2]	"The complete genome sequence of Dictyoglomus thermophilum strain ATCC 35947 / DSM 3960 / H-6-12." Dodson R.J., Durkin A.S., Wu M., Eisen J., Sutton G. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35947 / DSM 3960 / H-6-12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X07896 Genomic DNA. Translation: CAA30735.1. CP001146 Genomic DNA. Translation: ACI18637.1.
PIR	ALDYAT. S00628.
RefSeq	YP_002250612.1. NC_011297.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	309799.DICTH_0745.
Protein family/group databases
CAZy	GH57. Glycoside Hydrolase Family 57.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACI18637; ACI18637; DICTH_0745.
GeneID	6945629.
KEGG	dth:DICTH_0745.
PATRIC	21806130. VBIDicThe33784_0734.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1449.
HOGENOM	HOG000223207.
KO	K07405.
OMA	HFMSAGL.
ProtClustDB	CLSK2480645.
Enzyme and pathway databases
BioCyc	DTHE309799:GHF9-383-MONOMER.
Family and domain databases
Gene3D	2.70.98.10. 1 hit. 3.20.110.10. 1 hit.
InterPro	IPR015179. A-amylase/a-glucTrfase_C. IPR015178. A-amylase/a-glucTrfase_central. IPR011013. Gal_mutarotase_SF_dom. IPR014718. Glyco_hydro-type_carb-bd_sub. IPR011330. Glyco_hydro/deAcase_b/a-brl. IPR027291. Glyco_hydro_38/57_N. IPR004300. Glyco_hydro_57_N. [Graphical view]
Pfam	PF09094. DUF1925. 1 hit. PF09095. DUF1926. 1 hit. PF03065. Glyco_hydro_57. 1 hit. [Graphical view]
ProDom	PD014405. DUF1925. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF74650. Gal_mut_like. 1 hit. SSF88713. Glyco_hydro/deAcase_b/a-brl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AMY1_DICT6

Accession

Primary (citable) accession number: P09961
Secondary accession number(s): B5YDK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program