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P09959 (SWI6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 158. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Regulatory protein SWI6
Alternative name(s):
Cell-cycle box factor subunit SWI6
MBF subunit P90
Trans-acting activator of HO endonuclease gene
Gene names
Name:SWI6
Ordered Locus Names:YLR182W
ORF Names:L9470.8
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length803 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Part of a complex involved in cell-cycle-dependent transcription. SWI4 and SWI6 are required for formation of the cell-cycle box factor-DNA complex. The repeated element in the upstream region of HO (5'-CACGAAAA-3') is called the cell cycle box (CCB).

Subunit structure

Component of the transcription complex MCB-binding factor (MBF) composed of SWI6 and MBP1. Component of the transcription complex SCB-binding factor (SBF) composed of SWI6 and SWI4. Interacts with MSA1 and STB1. Ref.5 Ref.11

Subcellular location

Nucleus. Cytoplasm.

Post-translational modification

Phosphorylated by CDC28 and dephosphorylated by CDC14. Ref.8

Miscellaneous

Present with 3340 molecules/cell in log phase SD medium.

Sequence similarities

Contains 5 ANK repeats.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MBP1P396785EBI-18641,EBI-10485
SWI4P253027EBI-18641,EBI-18626

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 803803Regulatory protein SWI6
PRO_0000067071

Regions

Repeat318 – 34629ANK 1
Repeat347 – 38337ANK 2
Repeat384 – 46986ANK 3
Repeat470 – 49829ANK 4
Repeat499 – 51416ANK 5
Compositional bias631 – 64010Glu-rich (acidic)

Amino acid modifications

Modified residue1491Phosphoserine Ref.13
Modified residue1601Phosphoserine; by CDC28 Ref.8 Ref.10
Modified residue1761Phosphoserine Ref.12 Ref.13
Modified residue1791Phosphothreonine Ref.9 Ref.13
Modified residue1821Phosphothreonine Ref.9 Ref.13
Modified residue5471Phosphoserine Ref.10

Secondary structure

........................................................... 803
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P09959 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 9B317FCACEEC493C

FASTA80390,560
        10         20         30         40         50         60 
MALEEVVRYL GPHNEIPLTL TRDSETGHFL LKHFLPILQQ YHDTGNINET NPDSFPTDEE 

        70         80         90        100        110        120 
RNKLLAHYGI AVNTDDRGEL WIELEKCLQL LNMLNLFGLF QDAFEFEEPE TDQDEEDPSH 

       130        140        150        160        170        180 
SKLPENKTKS ENSKDNISSK RINNLQDMSL DSDAHRELGS PLKKLKIDTS VIDAESDSTP 

       190        200        210        220        230        240 
NTARGKPNDD INKGPSGDNE NNGTDDNDRT AGPIITFTHD LTSDFLSSPL KIMKALPSPV 

       250        260        270        280        290        300 
VNDNEQKMKL EAFLQRLLFP EIQEMPTSLN NDSSNRNSEG GSSNQQQQHV SFDSLLQEVN 

       310        320        330        340        350        360 
DAFPNTQLNL NIPVDEHGNT PLHWLTSIAN LELVKHLVKH GSNRLYGDNM GESCLVKAVK 

       370        380        390        400        410        420 
SVNNYDSGTF EALLDYLYPC LILEDSMNRT ILHHIIITSG MTGCSAAAKY YLDILMGWIV 

       430        440        450        460        470        480 
KKQNRPIQSG TNEKESKPND KNGERKDSIL ENLDLKWIIA NMLNAQDSNG DTCLNIAARL 

       490        500        510        520        530        540 
GNISIVDALL DYGADPFIAN KSGLRPVDFG AGTSKLQNTN GGDENSKMVS KGDYDGQKNG 

       550        560        570        580        590        600 
KAKKIRSQLL KNPPETTSLI NDVQNLLNSI SKDYENETVQ YNEKLEKLHK ELNEQREELA 

       610        620        630        640        650        660 
NSREQLANVK QLKDEYSLMQ EQLTNLKAGI EEEEESFREE SKKLGIIADE SSGIDWDSSE 

       670        680        690        700        710        720 
YDADEPFKVE FLSDFLEDKL QKNYEGDISK LLEAESKEQI MEQIRNQLPA EKIQSMLPPT 

       730        740        750        760        770        780 
VLLKARINAY KRNDKHLTNV LDTISTKQSE LENKFRRVLS LCLKIDENKV DNMLDGLLQA 

       790        800 
ISSEDPQDID TDEMQDFLKK HAS 

« Hide

References

« Hide 'large scale' references
[1]"Similarity between cell-cycle genes of budding yeast and fission yeast and the Notch gene of Drosophila."
Breeden L., Nasmyth K.
Nature 329:651-654(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A central role for SWI6 in modulating cell cycle Start-specific transcription in yeast."
Dirick L., Moll T., Auer H., Nasmyth K.
Nature 357:508-513(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Regulation of transcription at the Saccharomyces cerevisiae start transition by Stb1, a Swi6-binding protein."
Ho Y., Costanzo M., Moore L., Kobayashi R., Andrews B.J.
Mol. Cell. Biol. 19:5267-5278(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH STB1.
[6]"Cell cycle activation of the Swi6p transcription factor is linked to nucleocytoplasmic shuttling."
Queralt E., Igual J.C.
Mol. Cell. Biol. 23:3126-3140(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOCYTOPLASMIC SHUTTLING.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Clb6/Cdc28 and Cdc14 regulate phosphorylation status and cellular localization of Swi6."
Geymonat M., Spanos A., Wells G.P., Smerdon S.J., Sedgwick S.G.
Mol. Cell. Biol. 24:2277-2285(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-160.
[9]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-179 AND THR-182, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[10]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-160 AND SER-547, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"The SBF- and MBF-associated protein Msa1 is required for proper timing of G1-specific transcription in Saccharomyces cerevisiae."
Ashe M., de Bruin R.A.M., Kalashnikova T., McDonald W.H., Yates J.R. III, Wittenberg C.
J. Biol. Chem. 283:6040-6049(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MSA1.
[12]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-149; SER-176; THR-179 AND THR-182, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]"X-ray structural analysis of the yeast cell cycle regulator Swi6 reveals variations of the ankyrin fold and has implications for Swi6 function."
Foord R., Taylor I.A., Sedgwick S.G., Smerdon S.J.
Nat. Struct. Biol. 6:157-165(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 188-514.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X06238 Genomic DNA. Translation: CAA29581.1.
U17246 Genomic DNA. Translation: AAB67460.1.
BK006945 Genomic DNA. Translation: DAA09502.1.
PIRRGBYW6. S03161.
RefSeqNP_013283.1. NM_001182069.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1SW6X-ray2.10A/B188-512[»]
2XFVX-ray1.90A/B2-126[»]
ProteinModelPortalP09959.
SMRP09959. Positions 2-109, 212-512.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31453. 391 interactions.
DIPDIP-598N.
IntActP09959. 55 interactions.
MINTMINT-528932.
STRING4932.YLR182W.

Proteomic databases

PaxDbP09959.
PeptideAtlasP09959.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR182W; YLR182W; YLR182W.
GeneID850879.
KEGGsce:YLR182W.

Organism-specific databases

CYGDYLR182w.
SGDS000004172. SWI6.

Phylogenomic databases

eggNOGCOG0666.
GeneTreeENSGT00530000067565.
HOGENOMHOG000057104.
KOK06648.
OMAYYLDILM.
OrthoDBEOG73BVP1.

Enzyme and pathway databases

BioCycYEAST:G3O-32306-MONOMER.

Gene expression databases

GenevestigatorP09959.

Family and domain databases

Gene3D1.25.40.20. 2 hits.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamPF00023. Ank. 1 hit.
[Graphical view]
SMARTSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMSSF48403. SSF48403. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 2 hits.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP09959.
NextBio967230.

Entry information

Entry nameSWI6_YEAST
AccessionPrimary (citable) accession number: P09959
Secondary accession number(s): D6VYI6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 158 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references