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P09959

- SWI6_YEAST

UniProt

P09959 - SWI6_YEAST

Protein

Regulatory protein SWI6

Gene

SWI6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 161 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Part of a complex involved in cell-cycle-dependent transcription. SWI4 and SWI6 are required for formation of the cell-cycle box factor-DNA complex. The repeated element in the upstream region of HO (5'-CACGAAAA-3') is called the cell cycle box (CCB).

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. RNA polymerase II transcription coactivator activity Source: SGD

    GO - Biological processi

    1. positive regulation of reciprocal meiotic recombination Source: SGD
    2. positive regulation of transcription from RNA polymerase II promoter Source: SGD
    3. positive regulation of transcription from RNA polymerase II promoter in response to heat stress Source: SGD
    4. positive regulation of transcription involved in G1/S transition of mitotic cell cycle Source: SGD

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32306-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Regulatory protein SWI6
    Alternative name(s):
    Cell-cycle box factor subunit SWI6
    MBF subunit P90
    Trans-acting activator of HO endonuclease gene
    Gene namesi
    Name:SWI6
    Ordered Locus Names:YLR182W
    ORF Names:L9470.8
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR182w.
    SGDiS000004172. SWI6.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. MBF transcription complex Source: SGD
    3. nucleus Source: SGD
    4. SBF transcription complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 803803Regulatory protein SWI6PRO_0000067071Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei149 – 1491Phosphoserine1 Publication
    Modified residuei160 – 1601Phosphoserine; by CDC282 Publications
    Modified residuei176 – 1761Phosphoserine2 Publications
    Modified residuei179 – 1791Phosphothreonine2 Publications
    Modified residuei182 – 1821Phosphothreonine2 Publications
    Modified residuei547 – 5471Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylated by CDC28 and dephosphorylated by CDC14.5 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP09959.
    PaxDbiP09959.
    PeptideAtlasiP09959.

    Expressioni

    Gene expression databases

    GenevestigatoriP09959.

    Interactioni

    Subunit structurei

    Component of the transcription complex MCB-binding factor (MBF) composed of SWI6 and MBP1. Component of the transcription complex SCB-binding factor (SBF) composed of SWI6 and SWI4. Interacts with MSA1 and STB1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MBP1P396785EBI-18641,EBI-10485
    SWI4P253027EBI-18641,EBI-18626

    Protein-protein interaction databases

    BioGridi31453. 391 interactions.
    DIPiDIP-598N.
    IntActiP09959. 55 interactions.
    MINTiMINT-528932.
    STRINGi4932.YLR182W.

    Structurei

    Secondary structure

    1
    803
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 119
    Beta strandi16 – 238
    Turni24 – 263
    Beta strandi29 – 313
    Helixi32 – 343
    Helixi35 – 4410
    Helixi47 – 493
    Helixi58 – 6811
    Beta strandi80 – 823
    Helixi84 – 9310
    Turni97 – 1004
    Helixi101 – 1033
    Beta strandi104 – 1063
    Beta strandi214 – 2163
    Turni225 – 2273
    Helixi244 – 25815
    Helixi292 – 30211
    Helixi321 – 3277
    Helixi331 – 3399
    Helixi354 – 3607
    Helixi363 – 3664
    Helixi370 – 3778
    Helixi378 – 3825
    Helixi391 – 3999
    Helixi405 – 42117
    Helixi422 – 4243
    Beta strandi427 – 4293
    Helixi448 – 4525
    Helixi455 – 4617
    Turni462 – 4643
    Helixi473 – 4808
    Helixi483 – 4919
    Helixi506 – 5094

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1SW6X-ray2.10A/B188-512[»]
    2XFVX-ray1.90A/B2-126[»]
    ProteinModelPortaliP09959.
    SMRiP09959. Positions 2-109, 212-512.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP09959.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati318 – 34629ANK 1Add
    BLAST
    Repeati347 – 38337ANK 2Add
    BLAST
    Repeati384 – 46986ANK 3Add
    BLAST
    Repeati470 – 49829ANK 4Add
    BLAST
    Repeati499 – 51416ANK 5Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi631 – 64010Glu-rich (acidic)

    Sequence similaritiesi

    Contains 5 ANK repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    ANK repeat, Repeat

    Phylogenomic databases

    eggNOGiCOG0666.
    GeneTreeiENSGT00530000067565.
    HOGENOMiHOG000057104.
    KOiK06648.
    OMAiYYLDILM.
    OrthoDBiEOG73BVP1.

    Family and domain databases

    Gene3Di1.25.40.20. 2 hits.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    [Graphical view]
    PfamiPF00023. Ank. 1 hit.
    [Graphical view]
    SMARTiSM00248. ANK. 2 hits.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 2 hits.
    PS50088. ANK_REPEAT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P09959-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALEEVVRYL GPHNEIPLTL TRDSETGHFL LKHFLPILQQ YHDTGNINET    50
    NPDSFPTDEE RNKLLAHYGI AVNTDDRGEL WIELEKCLQL LNMLNLFGLF 100
    QDAFEFEEPE TDQDEEDPSH SKLPENKTKS ENSKDNISSK RINNLQDMSL 150
    DSDAHRELGS PLKKLKIDTS VIDAESDSTP NTARGKPNDD INKGPSGDNE 200
    NNGTDDNDRT AGPIITFTHD LTSDFLSSPL KIMKALPSPV VNDNEQKMKL 250
    EAFLQRLLFP EIQEMPTSLN NDSSNRNSEG GSSNQQQQHV SFDSLLQEVN 300
    DAFPNTQLNL NIPVDEHGNT PLHWLTSIAN LELVKHLVKH GSNRLYGDNM 350
    GESCLVKAVK SVNNYDSGTF EALLDYLYPC LILEDSMNRT ILHHIIITSG 400
    MTGCSAAAKY YLDILMGWIV KKQNRPIQSG TNEKESKPND KNGERKDSIL 450
    ENLDLKWIIA NMLNAQDSNG DTCLNIAARL GNISIVDALL DYGADPFIAN 500
    KSGLRPVDFG AGTSKLQNTN GGDENSKMVS KGDYDGQKNG KAKKIRSQLL 550
    KNPPETTSLI NDVQNLLNSI SKDYENETVQ YNEKLEKLHK ELNEQREELA 600
    NSREQLANVK QLKDEYSLMQ EQLTNLKAGI EEEEESFREE SKKLGIIADE 650
    SSGIDWDSSE YDADEPFKVE FLSDFLEDKL QKNYEGDISK LLEAESKEQI 700
    MEQIRNQLPA EKIQSMLPPT VLLKARINAY KRNDKHLTNV LDTISTKQSE 750
    LENKFRRVLS LCLKIDENKV DNMLDGLLQA ISSEDPQDID TDEMQDFLKK 800
    HAS 803
    Length:803
    Mass (Da):90,560
    Last modified:July 1, 1989 - v1
    Checksum:i9B317FCACEEC493C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06238 Genomic DNA. Translation: CAA29581.1.
    U17246 Genomic DNA. Translation: AAB67460.1.
    BK006945 Genomic DNA. Translation: DAA09502.1.
    PIRiS03161. RGBYW6.
    RefSeqiNP_013283.1. NM_001182069.1.

    Genome annotation databases

    EnsemblFungiiYLR182W; YLR182W; YLR182W.
    GeneIDi850879.
    KEGGisce:YLR182W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06238 Genomic DNA. Translation: CAA29581.1 .
    U17246 Genomic DNA. Translation: AAB67460.1 .
    BK006945 Genomic DNA. Translation: DAA09502.1 .
    PIRi S03161. RGBYW6.
    RefSeqi NP_013283.1. NM_001182069.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1SW6 X-ray 2.10 A/B 188-512 [» ]
    2XFV X-ray 1.90 A/B 2-126 [» ]
    ProteinModelPortali P09959.
    SMRi P09959. Positions 2-109, 212-512.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31453. 391 interactions.
    DIPi DIP-598N.
    IntActi P09959. 55 interactions.
    MINTi MINT-528932.
    STRINGi 4932.YLR182W.

    Proteomic databases

    MaxQBi P09959.
    PaxDbi P09959.
    PeptideAtlasi P09959.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR182W ; YLR182W ; YLR182W .
    GeneIDi 850879.
    KEGGi sce:YLR182W.

    Organism-specific databases

    CYGDi YLR182w.
    SGDi S000004172. SWI6.

    Phylogenomic databases

    eggNOGi COG0666.
    GeneTreei ENSGT00530000067565.
    HOGENOMi HOG000057104.
    KOi K06648.
    OMAi YYLDILM.
    OrthoDBi EOG73BVP1.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32306-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P09959.
    NextBioi 967230.

    Gene expression databases

    Genevestigatori P09959.

    Family and domain databases

    Gene3Di 1.25.40.20. 2 hits.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    [Graphical view ]
    Pfami PF00023. Ank. 1 hit.
    [Graphical view ]
    SMARTi SM00248. ANK. 2 hits.
    [Graphical view ]
    SUPFAMi SSF48403. SSF48403. 1 hit.
    PROSITEi PS50297. ANK_REP_REGION. 2 hits.
    PS50088. ANK_REPEAT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Similarity between cell-cycle genes of budding yeast and fission yeast and the Notch gene of Drosophila."
      Breeden L., Nasmyth K.
      Nature 329:651-654(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A central role for SWI6 in modulating cell cycle Start-specific transcription in yeast."
      Dirick L., Moll T., Auer H., Nasmyth K.
      Nature 357:508-513(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. "Regulation of transcription at the Saccharomyces cerevisiae start transition by Stb1, a Swi6-binding protein."
      Ho Y., Costanzo M., Moore L., Kobayashi R., Andrews B.J.
      Mol. Cell. Biol. 19:5267-5278(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH STB1.
    6. "Cell cycle activation of the Swi6p transcription factor is linked to nucleocytoplasmic shuttling."
      Queralt E., Igual J.C.
      Mol. Cell. Biol. 23:3126-3140(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOCYTOPLASMIC SHUTTLING.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Clb6/Cdc28 and Cdc14 regulate phosphorylation status and cellular localization of Swi6."
      Geymonat M., Spanos A., Wells G.P., Smerdon S.J., Sedgwick S.G.
      Mol. Cell. Biol. 24:2277-2285(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-160.
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-179 AND THR-182, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-160 AND SER-547, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "The SBF- and MBF-associated protein Msa1 is required for proper timing of G1-specific transcription in Saccharomyces cerevisiae."
      Ashe M., de Bruin R.A.M., Kalashnikova T., McDonald W.H., Yates J.R. III, Wittenberg C.
      J. Biol. Chem. 283:6040-6049(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MSA1.
    12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-149; SER-176; THR-179 AND THR-182, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    15. "X-ray structural analysis of the yeast cell cycle regulator Swi6 reveals variations of the ankyrin fold and has implications for Swi6 function."
      Foord R., Taylor I.A., Sedgwick S.G., Smerdon S.J.
      Nat. Struct. Biol. 6:157-165(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 188-514.

    Entry informationi

    Entry nameiSWI6_YEAST
    AccessioniPrimary (citable) accession number: P09959
    Secondary accession number(s): D6VYI6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 161 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3340 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3