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Protein

Carboxypeptidase B

Gene

CPB1

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential release of a C-terminal lysine or arginine amino acid.By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi176Zinc; catalyticBy similarity1
Metal bindingi179Zinc; catalyticBy similarity1
Binding sitei234SubstrateBy similarity1
Metal bindingi304Zinc; catalyticBy similarity1
Binding sitei356SubstrateBy similarity1
Active sitei378Proton donor/acceptorBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.17.2. 6170.

Protein family/group databases

MEROPSiM14.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase B (EC:3.4.17.2By similarity)
Gene namesi
Name:CPB1
Synonyms:CPB
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

  • Secreted By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4065.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 152 PublicationsAdd BLAST15
PropeptideiPRO_000000437316 – 110Activation peptideAdd BLAST95
ChainiPRO_0000004374111 – 416Carboxypeptidase BAdd BLAST306

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi173 ↔ 1861 Publication
Disulfide bondi245 ↔ 2681 Publication
Disulfide bondi259 ↔ 2731 Publication

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP09955.
PeptideAtlasiP09955.
PRIDEiP09955.

Miscellaneous databases

PMAP-CutDBP09955.

Interactioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000012464.

Chemistry databases

BindingDBiP09955.

Structurei

Secondary structure

1416
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi18 – 21Combined sources4
Beta strandi26 – 32Combined sources7
Helixi35 – 47Combined sources13
Beta strandi50 – 57Combined sources8
Helixi58 – 60Combined sources3
Beta strandi63 – 71Combined sources9
Helixi73 – 75Combined sources3
Helixi76 – 85Combined sources10
Beta strandi90 – 95Combined sources6
Helixi97 – 103Combined sources7
Beta strandi116 – 118Combined sources3
Helixi122 – 135Combined sources14
Turni137 – 139Combined sources3
Beta strandi140 – 147Combined sources8
Beta strandi153 – 160Combined sources8
Beta strandi168 – 173Combined sources6
Helixi180 – 196Combined sources17
Turni197 – 199Combined sources3
Helixi201 – 209Combined sources9
Beta strandi211 – 216Combined sources6
Helixi220 – 228Combined sources9
Beta strandi241 – 244Combined sources4
Helixi250 – 252Combined sources3
Beta strandi254 – 257Combined sources4
Beta strandi260 – 262Combined sources3
Beta strandi263 – 265Combined sources3
Helixi281 – 292Combined sources12
Turni293 – 296Combined sources4
Beta strandi297 – 304Combined sources8
Beta strandi309 – 313Combined sources5
Beta strandi315 – 318Combined sources4
Helixi324 – 339Combined sources16
Turni340 – 342Combined sources3
Beta strandi347 – 350Combined sources4
Helixi351 – 354Combined sources4
Helixi362 – 368Combined sources7
Beta strandi372 – 378Combined sources7
Beta strandi382 – 385Combined sources4
Helixi386 – 388Combined sources3
Helixi391 – 393Combined sources3
Helixi394 – 414Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NSAX-ray2.30A41-416[»]
1PBANMR-A16-96[»]
1Z5RX-ray1.40A/B/C111-416[»]
1ZG7X-ray1.75A/B/C111-416[»]
1ZG8X-ray2.00A/B/C111-416[»]
1ZG9X-ray2.00A/B/C111-416[»]
2JEWX-ray1.40A111-416[»]
2PIYX-ray1.43A/B/C111-416[»]
2PIZX-ray1.60A/B/C111-416[»]
2PJ0X-ray1.65A/B/C111-416[»]
2PJ1X-ray1.64A/B/C111-416[»]
2PJ2X-ray1.95A/B/C111-416[»]
2PJ3X-ray1.64A/B/C111-416[»]
2PJ4X-ray2.00A/B111-416[»]
2PJ5X-ray1.65A/B/C111-416[»]
2PJ6X-ray1.60A111-416[»]
2PJ7X-ray1.77A/B/C111-416[»]
2PJ8X-ray1.70A/B/C111-416[»]
2PJ9X-ray1.56A111-416[»]
2PJAX-ray1.70A/B/C111-416[»]
2PJBX-ray1.70A/B/C111-416[»]
2PJCX-ray1.74A/B/C111-416[»]
3GLJX-ray1.89A16-416[»]
3WABX-ray2.15A111-416[»]
3WC5X-ray1.70A111-416[»]
3WC6X-ray1.65A111-416[»]
3WC7X-ray1.90A111-416[»]
4UIAX-ray2.18A111-416[»]
4UIBX-ray1.94A111-416[»]
4Z65X-ray1.25A113-416[»]
5LRGX-ray2.02A/B/C111-416[»]
5LRJX-ray2.20A/B/C111-416[»]
5LRKX-ray2.30A/B/C111-416[»]
ProteinModelPortaliP09955.
SMRiP09955.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09955.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni176 – 179Substrate bindingBy similarity4
Regioni251 – 252Substrate bindingBy similarity2
Regioni305 – 306Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2650. Eukaryota.
COG2866. LUCA.
HOGENOMiHOG000252968.
HOVERGENiHBG050815.
InParanoidiP09955.
KOiK01291.

Family and domain databases

Gene3Di3.30.70.340. 1 hit.
InterProiIPR003146. M14A_act_pep.
IPR000834. Peptidase_M14.
IPR009020. Propept_inh.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09955-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAFLILVTV TLASAHHSGE HFEGEKVFRV NVEDENDISL LHELASTRQI
60 70 80 90 100
DFWKPDSVTQ IKPHSTVDFR VKAEDILAVE DFLEQNELQY EVLINNLRSV
110 120 130 140 150
LEAQFDSRVR TTGHSYEKYN NWETIEAWTK QVTSENPDLI SRTAIGTTFL
160 170 180 190 200
GNNIYLLKVG KPGPNKPAIF MDCGFHAREW ISHAFCQWFV REAVLTYGYE
210 220 230 240 250
SHMTEFLNKL DFYVLPVLNI DGYIYTWTKN RMWRKTRSTN AGTTCIGTDP
260 270 280 290 300
NRNFDAGWCT TGASTDPCDE TYCGSAAESE KETKALADFI RNNLSSIKAY
310 320 330 340 350
LTIHSYSQMI LYPYSYDYKL PENNAELNNL AKAAVKELAT LYGTKYTYGP
360 370 380 390 400
GATTIYPAAG GSDDWAYDQG IKYSFTFELR DKGRYGFILP ESQIQATCEE
410
TMLAIKYVTN YVLGHL
Length:416
Mass (Da):47,381
Last modified:April 26, 2005 - v5
Checksum:i5FA7FCED7B45AE6A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40L → E AA sequence (PubMed:2018774).Curated1
Sequence conflicti40L → E AA sequence (PubMed:4026847).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ133775 mRNA. Translation: CAB46991.1.
PIRiA38354.
RefSeqiNP_999334.1. NM_214169.1.
UniGeneiSsc.16048.

Genome annotation databases

GeneIDi397341.
KEGGissc:397341.

Cross-referencesi

Web resourcesi

Worthington enzyme manual

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ133775 mRNA. Translation: CAB46991.1.
PIRiA38354.
RefSeqiNP_999334.1. NM_214169.1.
UniGeneiSsc.16048.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NSAX-ray2.30A41-416[»]
1PBANMR-A16-96[»]
1Z5RX-ray1.40A/B/C111-416[»]
1ZG7X-ray1.75A/B/C111-416[»]
1ZG8X-ray2.00A/B/C111-416[»]
1ZG9X-ray2.00A/B/C111-416[»]
2JEWX-ray1.40A111-416[»]
2PIYX-ray1.43A/B/C111-416[»]
2PIZX-ray1.60A/B/C111-416[»]
2PJ0X-ray1.65A/B/C111-416[»]
2PJ1X-ray1.64A/B/C111-416[»]
2PJ2X-ray1.95A/B/C111-416[»]
2PJ3X-ray1.64A/B/C111-416[»]
2PJ4X-ray2.00A/B111-416[»]
2PJ5X-ray1.65A/B/C111-416[»]
2PJ6X-ray1.60A111-416[»]
2PJ7X-ray1.77A/B/C111-416[»]
2PJ8X-ray1.70A/B/C111-416[»]
2PJ9X-ray1.56A111-416[»]
2PJAX-ray1.70A/B/C111-416[»]
2PJBX-ray1.70A/B/C111-416[»]
2PJCX-ray1.74A/B/C111-416[»]
3GLJX-ray1.89A16-416[»]
3WABX-ray2.15A111-416[»]
3WC5X-ray1.70A111-416[»]
3WC6X-ray1.65A111-416[»]
3WC7X-ray1.90A111-416[»]
4UIAX-ray2.18A111-416[»]
4UIBX-ray1.94A111-416[»]
4Z65X-ray1.25A113-416[»]
5LRGX-ray2.02A/B/C111-416[»]
5LRJX-ray2.20A/B/C111-416[»]
5LRKX-ray2.30A/B/C111-416[»]
ProteinModelPortaliP09955.
SMRiP09955.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000012464.

Chemistry databases

BindingDBiP09955.
ChEMBLiCHEMBL4065.

Protein family/group databases

MEROPSiM14.003.

Proteomic databases

PaxDbiP09955.
PeptideAtlasiP09955.
PRIDEiP09955.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi397341.
KEGGissc:397341.

Organism-specific databases

CTDi1360.

Phylogenomic databases

eggNOGiKOG2650. Eukaryota.
COG2866. LUCA.
HOGENOMiHOG000252968.
HOVERGENiHBG050815.
InParanoidiP09955.
KOiK01291.

Enzyme and pathway databases

BRENDAi3.4.17.2. 6170.

Miscellaneous databases

EvolutionaryTraceiP09955.
PMAP-CutDBP09955.
PROiP09955.

Family and domain databases

Gene3Di3.30.70.340. 1 hit.
InterProiIPR003146. M14A_act_pep.
IPR000834. Peptidase_M14.
IPR009020. Propept_inh.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBPB1_PIG
AccessioniPrimary (citable) accession number: P09955
Secondary accession number(s): Q9XSP3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: April 26, 2005
Last modified: November 2, 2016
This is version 141 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.