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Protein

Carboxypeptidase A1

Gene

CPA1

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro.By similarity

Catalytic activityi

Release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro.

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi179 – 1791Zinc; catalyticCombined sources
Metal bindingi182 – 1821Zinc; catalyticCombined sources
Binding sitei237 – 2371SubstrateBy similarity
Metal bindingi306 – 3061Zinc; catalyticCombined sources
Binding sitei358 – 3581SubstrateBy similarity
Active sitei380 – 3801Proton donor/acceptorBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM14.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase A1 (EC:3.4.17.1)
Gene namesi
Name:CPA1
Synonyms:CPA
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 18

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 16161 PublicationAdd
BLAST
Propeptidei17 – 11094Activation peptidePRO_0000004349Add
BLAST
Chaini111 – 419309Carboxypeptidase A1PRO_0000004350Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi248 ↔ 271Combined sources

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP09954.

Expressioni

Gene expression databases

GenevisibleiP09954. SS.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000017542.

Structurei

Secondary structure

1
419
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi24 – 285Combined sources
Helixi33 – 4210Combined sources
Helixi46 – 483Combined sources
Beta strandi51 – 544Combined sources
Beta strandi63 – 675Combined sources
Helixi69 – 713Combined sources
Helixi72 – 8110Combined sources
Beta strandi86 – 916Combined sources
Helixi93 – 10614Combined sources
Helixi125 – 13814Combined sources
Turni140 – 1423Combined sources
Beta strandi143 – 1508Combined sources
Beta strandi156 – 1627Combined sources
Beta strandi171 – 1766Combined sources
Helixi183 – 19917Combined sources
Turni200 – 2023Combined sources
Helixi204 – 2107Combined sources
Beta strandi214 – 2196Combined sources
Helixi223 – 2319Combined sources
Helixi253 – 2553Combined sources
Beta strandi257 – 2604Combined sources
Beta strandi266 – 2683Combined sources
Helixi284 – 29613Combined sources
Beta strandi299 – 3068Combined sources
Beta strandi308 – 3158Combined sources
Helixi326 – 34419Combined sources
Beta strandi349 – 3524Combined sources
Helixi353 – 3564Combined sources
Helixi364 – 3707Combined sources
Beta strandi375 – 3806Combined sources
Beta strandi384 – 3874Combined sources
Helixi393 – 3953Combined sources
Helixi396 – 41621Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PCAX-ray2.00A17-416[»]
ProteinModelPortaliP09954.
SMRiP09954. Positions 17-419.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09954.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni179 – 1824Substrate bindingBy similarity
Regioni254 – 2552Substrate bindingBy similarity
Regioni307 – 3082Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2650. Eukaryota.
COG2866. LUCA.
GeneTreeiENSGT00760000119103.
HOGENOMiHOG000252967.
HOVERGENiHBG050815.
InParanoidiP09954.
KOiK08779.
OMAiSHGISYE.
OrthoDBiEOG7RZ5Q9.
TreeFamiTF317197.

Family and domain databases

Gene3Di3.30.70.340. 1 hit.
InterProiIPR000834. Peptidase_M14.
IPR003146. Prot_inh_M14A.
IPR009020. Prot_inh_propept.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWGLLIFSVL LGGVLAKEDF VGHQVLRISV DDEAQVQKVK ELEDLEHLQL
60 70 80 90 100
DFWRGPARPG FPIDVRVPFP SIQAVKVFLE AHGIRYTIMI EDVQLLLDEE
110 120 130 140 150
QEQMFASQGR ARTTSTFNYA TYHTLEEIYD FMDILVAEHP QLVSKLQIGS
160 170 180 190 200
SYEGRPIYVL KFSTGGNNRP AIWIDTGIHS REWVTQASGV WFAKKITEDY
210 220 230 240 250
GQDPAFTAIL DNLDIFLEIV TNPDGFAFTH SENRMWRKTR SRTSGSFCVG
260 270 280 290 300
VDPNRNWDAG FGGAGASSNP CSETYHGKFP NSEVEVKSIV DFVNDHGNIK
310 320 330 340 350
AFISIHSYSQ LLLYPYGYKT EAPADKDELD QISKSAVAAL TSLYGTKFQY
360 370 380 390 400
GSIITTIYQA SGGTIDWTYN QGIKYSFSFE LRDTGRYGFL LPASQIIPTA
410
QETWLALLTI MEHTLNHPY
Length:419
Mass (Da):47,235
Last modified:November 7, 2003 - v2
Checksum:i84B4CB557B714FC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF076222 mRNA. Translation: AAD17690.1.
PIRiA25833.
RefSeqiNP_999409.1. NM_214244.1.
UniGeneiSsc.751.

Genome annotation databases

EnsembliENSSSCT00000018027; ENSSSCP00000017542; ENSSSCG00000016557.
GeneIDi397476.
KEGGissc:397476.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF076222 mRNA. Translation: AAD17690.1.
PIRiA25833.
RefSeqiNP_999409.1. NM_214244.1.
UniGeneiSsc.751.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PCAX-ray2.00A17-416[»]
ProteinModelPortaliP09954.
SMRiP09954. Positions 17-419.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000017542.

Protein family/group databases

MEROPSiM14.001.

Proteomic databases

PaxDbiP09954.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000018027; ENSSSCP00000017542; ENSSSCG00000016557.
GeneIDi397476.
KEGGissc:397476.

Organism-specific databases

CTDi1357.

Phylogenomic databases

eggNOGiKOG2650. Eukaryota.
COG2866. LUCA.
GeneTreeiENSGT00760000119103.
HOGENOMiHOG000252967.
HOVERGENiHBG050815.
InParanoidiP09954.
KOiK08779.
OMAiSHGISYE.
OrthoDBiEOG7RZ5Q9.
TreeFamiTF317197.

Miscellaneous databases

EvolutionaryTraceiP09954.

Gene expression databases

GenevisibleiP09954. SS.

Family and domain databases

Gene3Di3.30.70.340. 1 hit.
InterProiIPR000834. Peptidase_M14.
IPR003146. Prot_inh_M14A.
IPR009020. Prot_inh_propept.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning, sequencing and functional expression of a cDNA encoding porcine pancreatic preprocarboxypeptidase A1."
    Darnis S., Juge N., Marino C., Aviles F.X., Puigserver A., Chaix J.-C., Guo X.-J.
    Eur. J. Biochem. 259:719-725(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pancreas.
  2. "Primary structure of the activation segment of procarboxypeptidase A from porcine pancreas."
    Vendrell J., Aviles F.X., Genesca E., San Segundo B., Soriano F., Mendez E.
    Biochem. Biophys. Res. Commun. 141:517-523(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 17-110.
  3. "Three-dimensional structure of porcine pancreatic procarboxypeptidase A. A comparison of the A and B zymogens and their determinants for inhibition and activation."
    Guasch A., Coll M., Aviles F.X., Huber R.
    J. Mol. Biol. 224:141-157(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 17-416 IN COMPLEX WITH ZINC, DISULFIDE BONDS.

Entry informationi

Entry nameiCBPA1_PIG
AccessioniPrimary (citable) accession number: P09954
Secondary accession number(s): Q9TV85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 7, 2003
Last modified: February 17, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.