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Protein

Carboxypeptidase A1

Gene

CPA1

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro.By similarity

Catalytic activityi

Release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro.

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi179Zinc; catalyticCombined sources1
Metal bindingi182Zinc; catalyticCombined sources1
Binding sitei237SubstrateBy similarity1
Metal bindingi306Zinc; catalyticCombined sources1
Binding sitei358SubstrateBy similarity1
Active sitei380Proton donor/acceptorBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM14.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase A1 (EC:3.4.17.1)
Gene namesi
Name:CPA1
Synonyms:CPA
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 18

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 161 PublicationAdd BLAST16
PropeptideiPRO_000000434917 – 110Activation peptideAdd BLAST94
ChainiPRO_0000004350111 – 419Carboxypeptidase A1Add BLAST309

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi248 ↔ 271Combined sources

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP09954.
PeptideAtlasiP09954.
PRIDEiP09954.

Expressioni

Gene expression databases

GenevisibleiP09954. SS.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000017542.

Structurei

Secondary structure

1419
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi24 – 28Combined sources5
Helixi33 – 42Combined sources10
Helixi46 – 48Combined sources3
Beta strandi51 – 54Combined sources4
Beta strandi63 – 67Combined sources5
Helixi69 – 71Combined sources3
Helixi72 – 81Combined sources10
Beta strandi86 – 91Combined sources6
Helixi93 – 106Combined sources14
Helixi125 – 138Combined sources14
Turni140 – 142Combined sources3
Beta strandi143 – 150Combined sources8
Beta strandi156 – 162Combined sources7
Beta strandi171 – 176Combined sources6
Helixi183 – 199Combined sources17
Turni200 – 202Combined sources3
Helixi204 – 210Combined sources7
Beta strandi214 – 219Combined sources6
Helixi223 – 231Combined sources9
Helixi253 – 255Combined sources3
Beta strandi257 – 260Combined sources4
Beta strandi266 – 268Combined sources3
Helixi284 – 296Combined sources13
Beta strandi299 – 306Combined sources8
Beta strandi308 – 315Combined sources8
Helixi326 – 344Combined sources19
Beta strandi349 – 352Combined sources4
Helixi353 – 356Combined sources4
Helixi364 – 370Combined sources7
Beta strandi375 – 380Combined sources6
Beta strandi384 – 387Combined sources4
Helixi393 – 395Combined sources3
Helixi396 – 416Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PCAX-ray2.00A17-416[»]
ProteinModelPortaliP09954.
SMRiP09954.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09954.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni179 – 182Substrate bindingBy similarity4
Regioni254 – 255Substrate bindingBy similarity2
Regioni307 – 308Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2650. Eukaryota.
COG2866. LUCA.
GeneTreeiENSGT00760000119103.
HOGENOMiHOG000252967.
HOVERGENiHBG050815.
InParanoidiP09954.
KOiK08779.
OMAiSHGISYE.
OrthoDBiEOG091G0HUI.
TreeFamiTF317197.

Family and domain databases

Gene3Di3.30.70.340. 1 hit.
InterProiIPR003146. M14A_act_pep.
IPR000834. Peptidase_M14.
IPR009020. Propept_inh.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWGLLIFSVL LGGVLAKEDF VGHQVLRISV DDEAQVQKVK ELEDLEHLQL
60 70 80 90 100
DFWRGPARPG FPIDVRVPFP SIQAVKVFLE AHGIRYTIMI EDVQLLLDEE
110 120 130 140 150
QEQMFASQGR ARTTSTFNYA TYHTLEEIYD FMDILVAEHP QLVSKLQIGS
160 170 180 190 200
SYEGRPIYVL KFSTGGNNRP AIWIDTGIHS REWVTQASGV WFAKKITEDY
210 220 230 240 250
GQDPAFTAIL DNLDIFLEIV TNPDGFAFTH SENRMWRKTR SRTSGSFCVG
260 270 280 290 300
VDPNRNWDAG FGGAGASSNP CSETYHGKFP NSEVEVKSIV DFVNDHGNIK
310 320 330 340 350
AFISIHSYSQ LLLYPYGYKT EAPADKDELD QISKSAVAAL TSLYGTKFQY
360 370 380 390 400
GSIITTIYQA SGGTIDWTYN QGIKYSFSFE LRDTGRYGFL LPASQIIPTA
410
QETWLALLTI MEHTLNHPY
Length:419
Mass (Da):47,235
Last modified:November 7, 2003 - v2
Checksum:i84B4CB557B714FC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF076222 mRNA. Translation: AAD17690.1.
PIRiA25833.
RefSeqiNP_999409.1. NM_214244.1.
UniGeneiSsc.751.

Genome annotation databases

EnsembliENSSSCT00000018027; ENSSSCP00000017542; ENSSSCG00000016557.
GeneIDi397476.
KEGGissc:397476.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF076222 mRNA. Translation: AAD17690.1.
PIRiA25833.
RefSeqiNP_999409.1. NM_214244.1.
UniGeneiSsc.751.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PCAX-ray2.00A17-416[»]
ProteinModelPortaliP09954.
SMRiP09954.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000017542.

Protein family/group databases

MEROPSiM14.001.

Proteomic databases

PaxDbiP09954.
PeptideAtlasiP09954.
PRIDEiP09954.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000018027; ENSSSCP00000017542; ENSSSCG00000016557.
GeneIDi397476.
KEGGissc:397476.

Organism-specific databases

CTDi1357.

Phylogenomic databases

eggNOGiKOG2650. Eukaryota.
COG2866. LUCA.
GeneTreeiENSGT00760000119103.
HOGENOMiHOG000252967.
HOVERGENiHBG050815.
InParanoidiP09954.
KOiK08779.
OMAiSHGISYE.
OrthoDBiEOG091G0HUI.
TreeFamiTF317197.

Miscellaneous databases

EvolutionaryTraceiP09954.

Gene expression databases

GenevisibleiP09954. SS.

Family and domain databases

Gene3Di3.30.70.340. 1 hit.
InterProiIPR003146. M14A_act_pep.
IPR000834. Peptidase_M14.
IPR009020. Propept_inh.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBPA1_PIG
AccessioniPrimary (citable) accession number: P09954
Secondary accession number(s): Q9TV85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 7, 2003
Last modified: November 2, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.