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P09951 (SYN1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Synapsin-1
Alternative name(s):
Synapsin I
Gene names
Name:Syn1
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length704 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release.

Subunit structure

Homodimer By similarity. Interacts with CAPON. Forms a ternary complex with NOS1. Isoform Ib interacts with PRNP By similarity. Ref.6

Subcellular location

Cell junction › synapse. Golgi apparatus By similarity.

Post-translational modification

Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal By similarity.

Sequence similarities

Belongs to the synapsin family.

Sequence caution

The sequence CAA28353.1 differs from that shown. Reason: Sequencing errors.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform IA (identifier: P09951-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform IB (identifier: P09951-2)

The sequence of this isoform differs from the canonical sequence as follows:
     660-668: NKSQSLTNA → KASPAQAQP
     669-704: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 704704Synapsin-1
PRO_0000183020

Regions

Region1 – 2828A
Region29 – 11284B; linker
Region113 – 420308C; actin-binding and synaptic-vesicle binding
Region421 – 655235D; Pro-rich linker
Region656 – 70449E

Amino acid modifications

Modified residue91Phosphoserine; by CaMK1 and PKA
Modified residue391Phosphoserine By similarity
Modified residue621Phosphoserine By similarity
Modified residue671Phosphoserine By similarity
Modified residue3121Phosphotyrosine By similarity
Modified residue3371Phosphothreonine By similarity
Modified residue4251Phosphoserine By similarity
Modified residue5081Phosphoserine By similarity
Modified residue5101Phosphothreonine By similarity
Modified residue5181Phosphoserine By similarity
Modified residue5491Phosphoserine By similarity
Modified residue5511Phosphoserine; by PDPK1 By similarity
Modified residue5661Phosphoserine; by CaMK2
Modified residue6031Phosphoserine; by CaMK2
Modified residue6641Phosphoserine By similarity
Modified residue7031Phosphoserine By similarity
Glycosylation551O-linked (GlcNAc) Ref.4 Ref.5
Glycosylation561O-linked (GlcNAc) Probable
Glycosylation871O-linked (GlcNAc) Ref.4 Ref.5
Glycosylation961O-linked (GlcNAc) Ref.5
Glycosylation1031O-linked (GlcNAc) Probable
Glycosylation2611O-linked (GlcNAc) Ref.5
Glycosylation4301O-linked (GlcNAc) Ref.5
Glycosylation5161O-linked (GlcNAc) Ref.4 Ref.5
Glycosylation5241O-linked (GlcNAc) Ref.4
Glycosylation5621O-linked (GlcNAc) Ref.4
Glycosylation5761O-linked (GlcNAc) Ref.4

Natural variations

Alternative sequence660 – 6689NKSQSLTNA → KASPAQAQP in isoform IB.
VSP_006318
Alternative sequence669 – 70436Missing in isoform IB.
VSP_006319

Secondary structure

1 ...................................................... 704
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform IA [UniParc].

Last modified May 30, 2000. Version 3.
Checksum: 65799FEF7CFE18B5

FASTA70473,988
        10         20         30         40         50         60 
MNYLRRRLSD SNFMANLPNG YMTDLQRPQP PPPPPSAASP GATPGSAAAS AERASTAAPV 

        70         80         90        100        110        120 
ASPAAPSPGS SGGGGFFSSL SNAVKQTTAA AAATFSEQVG GGSGGAGRGG AAARVLLVID 

       130        140        150        160        170        180 
EPHTDWAKYF KGKKIHGEID IKVEQAEFSD LNLVAHANGG FSVDMEVLRN GVKVVRSLKP 

       190        200        210        220        230        240 
DFVLIRQHAF SMARNGDYRS LVIGLQYAGI PSVNSLHSVY NFCDKPWVFA QMVRLHKKLG 

       250        260        270        280        290        300 
TEEFPLIDQT FYPNHKEMLS STTYPVVVKM GHAHSGMGKV KVDNQHDFQD IASVVALTKT 

       310        320        330        340        350        360 
YATAEPFIDA KYDVRVQKIG QNYKAYMRTS VSGNWKTNTG SAMLEQIAMS DRYKLWVDTC 

       370        380        390        400        410        420 
SEIFGGLDIC AVEALHGKDG RDHIIEVVGS SMPLIGDHQD EDKQLIVELV VNKMTQALPR 

       430        440        450        460        470        480 
QRDASPGRGS HSQTPSPGAL PLGRQTSQQP AGPPAQQRPP PQGGPPQPGP GPQRQGPPLQ 

       490        500        510        520        530        540 
QRPPPQGQQH LSGLGPPAGS PLPQRLPSPT AAPQQSASQA TPMTQGQGRQ SRPVAGGPGA 

       550        560        570        580        590        600 
PPAARPPASP SPQRQAGPPQ ATRQASISGP APPKVSGASP GGQQRQGPPQ KPPGPAGPIR 

       610        620        630        640        650        660 
QASQAGPGPR TGPPTTQQPR PSGPGPAGRP TKPQLAQKPS QDVPPPIIAA AGGPPHPQLN 

       670        680        690        700 
KSQSLTNAFN LPEPAPPRPS LSQDEVKAET IRSLRKSFAS LFSD

« Hide

Isoform IB [UniParc].

Checksum: CE225AE8FCC92F8F
Show »

FASTA66869,910

References

[1]"Synapsins: mosaics of shared and individual domains in a family of synaptic vesicle phosphoproteins."
Suedhof T.C., Czernik A.J., Kao H.-T., Takei K., Johnston P.A., Horiuchi A., Kanazir S.D., Wagner M.A., Perin M.S., de Camilli P., Greengard P.
Science 245:1474-1480(1989) [PubMed: 2506642] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Determination and analysis of the primary structure of the nerve terminal specific phosphoprotein, synapsin I."
McCaffery C.A., Degennaro L.J.
EMBO J. 5:3167-3173(1986) [PubMed: 3028773] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]Lubec G., Diao W.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 177-186 AND 239-256, MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Hippocampus.
[4]"Glycosylation sites flank phosphorylation sites on synapsin I: O-linked N-acetylglucosamine residues are localized within domains mediating synapsin I interactions."
Cole R.N., Hart G.W.
J. Neurochem. 73:418-428(1999) [PubMed: 10386995] [Abstract]
Cited for: GLYCOSYLATION AT SER-55; THR-56; THR-87; SER-516; THR-524; THR-562 AND SER-576.
[5]"Mapping sites of O-GlcNAc modification using affinity tags for serine and threonine post-translational modifications."
Wells L., Vosseller K., Cole R.N., Cronshaw J.M., Matunis M.J., Hart G.W.
Mol. Cell. Proteomics 1:791-804(2002) [PubMed: 12438562] [Abstract]
Cited for: GLYCOSYLATION AT SER-55; THR-87; SER-96; SER-103; SER-261; SER-430 AND SER-516.
[6]"Neuronal nitric-oxide synthase localization mediated by a ternary complex with synapsin and CAPON."
Jaffrey S.R., Benfenati F., Snowman A.M., Czernik A.J., Snyder S.H.
Proc. Natl. Acad. Sci. U.S.A. 99:3199-3204(2002) [PubMed: 11867766] [Abstract]
Cited for: INTERACTION WITH NOS1 AND CAPON.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M27812 mRNA. Translation: AAA42145.1.
M27924 mRNA. Translation: AAA42148.1.
X04655 mRNA. Translation: CAA28353.1. Sequence problems.
IPIIPI00191335.
IPI00231023.
PIRA25704.
A30411.
RefSeqNP_001104252.1. NM_001110782.1.
NP_062006.1. NM_019133.1.
UniGeneRn.9923.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1PK8X-ray2.10A/B/C/D/E/F/G/H2-421[»]
1PX2X-ray2.23A/B2-421[»]
ProteinModelPortalP09951.
SMRP09951. Positions 112-417.
ModBaseSearch...

Protein-protein interaction databases

IntActP09951. 1 interaction.
MINTMINT-139278.
STRINGP09951.

PTM databases

PhosphoSiteP09951.

Proteomic databases

PRIDEP09951.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID24949.
KEGGrno:24949.
NMPDRfig|10116.3.peg.30766.

Organism-specific databases

CTD6853.
RGD3797. Syn1.

Phylogenomic databases

eggNOGmaNOG18102.
GeneTreeENSGT00530000063319.
HOVERGENHBG016354.
InParanoidP09951.
OrthoDBEOG4KPTB4.

Gene expression databases

ArrayExpressP09951.
GenevestigatorP09951.
GermOnlineENSRNOG00000010365. Rattus norvegicus.

Family and domain databases

InterProIPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR013817. Pre-ATP_grasp.
IPR016185. PreATP-grasp-like.
IPR001359. Synapsin.
IPR020898. Synapsin_ATP-bd_dom.
IPR019735. Synapsin_CS.
IPR019736. Synapsin_P_site.
IPR020897. Synapsin_pre-ATP-grasp_dom.
[Graphical view]
Gene3DG3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit.
G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 2 hits.
G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
PfamPF02078. Synapsin. 1 hit.
PF02750. Synapsin_C. 1 hit.
PF10581. Synapsin_N. 1 hit.
[Graphical view]
PRINTSPR01368. SYNAPSIN.
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
PROSITEPS00415. SYNAPSIN_1. 1 hit.
PS00416. SYNAPSIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio604961.

Entry information

Entry nameSYN1_RAT
AccessionPrimary (citable) accession number: P09951
Secondary accession number(s): Q9WUX7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: May 30, 2000
Last modified: January 25, 2012
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents