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Protein

Synapsin-1

Gene

Syn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release.

GO - Molecular functioni

  • ATP binding Source: InterPro
  • calcium-dependent protein binding Source: RGD
  • catalytic activity Source: InterPro

GO - Biological processi

  • neurotransmitter secretion Source: RGD
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Synapsin-1
Alternative name(s):
Synapsin I
Gene namesi
Name:Syn1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi3797. Syn1.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytosol Source: RGD
  • dendrite Source: BHF-UCL
  • Golgi apparatus Source: UniProtKB-SubCell
  • intracellular organelle Source: RGD
  • myelin sheath Source: Ensembl
  • postsynaptic density Source: Ensembl
  • presynaptic active zone Source: MGI
  • synapse Source: MGI
  • synaptic vesicle Source: UniProtKB
  • synaptic vesicle membrane Source: Ensembl
  • synaptonemal complex Source: Ensembl
  • terminal bouton Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Golgi apparatus, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001830201 – 704Synapsin-1Add BLAST704

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9Phosphoserine; by CaMK1 and PKA1 Publication1
Modified residuei39PhosphoserineCombined sources1
Glycosylationi55O-linked (GlcNAc)2 Publications1
Glycosylationi56O-linked (GlcNAc)1 Publication1
Modified residuei62PhosphoserineCombined sources1
Modified residuei67PhosphoserineCombined sources1
Glycosylationi87O-linked (GlcNAc)2 Publications1
Glycosylationi96O-linked (GlcNAc)1 Publication1
Glycosylationi103O-linked (GlcNAc)1 Publication1
Glycosylationi261O-linked (GlcNAc)1 Publication1
Modified residuei312PhosphotyrosineBy similarity1
Modified residuei425PhosphoserineCombined sources1
Modified residuei428Omega-N-methylarginineBy similarity1
Modified residuei430Phosphoserine; alternateBy similarity1
Glycosylationi430O-linked (GlcNAc); alternate1 Publication1
Modified residuei432PhosphoserineBy similarity1
Modified residuei434PhosphothreonineCombined sources1
Modified residuei436PhosphoserineCombined sources1
Modified residuei474Omega-N-methylarginineBy similarity1
Glycosylationi516O-linked (GlcNAc)2 Publications1
Glycosylationi524O-linked (GlcNAc)1 Publication1
Modified residuei532Omega-N-methylarginineBy similarity1
Modified residuei545Omega-N-methylarginineBy similarity1
Modified residuei549PhosphoserineBy similarity1
Modified residuei551Phosphoserine; by PDPK1By similarity1
Modified residuei554Omega-N-methylarginineBy similarity1
Glycosylationi562O-linked (GlcNAc)1 Publication1
Modified residuei566PhosphoserineCombined sources1
Modified residuei566Phosphoserine; by CaMK21 Publication1
Glycosylationi576O-linked (GlcNAc)1 Publication1
Modified residuei603Phosphoserine; by CaMK21 Publication1
Modified residuei620Omega-N-methylarginineBy similarity1
Modified residuei662PhosphoserineBy similarity1
Modified residuei664PhosphoserineCombined sources1
Modified residuei678Asymmetric dimethylarginineBy similarity1
Modified residuei682PhosphoserineBy similarity1

Post-translational modificationi

Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal (By similarity).By similarity
Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles.By similarity

Keywords - PTMi

Glycoprotein, Methylation, Phosphoprotein

Proteomic databases

PaxDbiP09951.
PRIDEiP09951.

PTM databases

iPTMnetiP09951.
PhosphoSitePlusiP09951.

Expressioni

Gene expression databases

BgeeiENSRNOG00000010365.
GenevisibleiP09951. RN.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with CAPON. Forms a ternary complex with NOS1. Isoform Ib interacts with PRNP (By similarity).By similarity

GO - Molecular functioni

  • calcium-dependent protein binding Source: RGD

Protein-protein interaction databases

BioGridi247051. 1 interactor.
IntActiP09951. 5 interactors.
MINTiMINT-139278.
STRINGi10116.ENSRNOP00000014250.

Structurei

Secondary structure

1704
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi114 – 119Combined sources6
Helixi126 – 130Combined sources5
Turni136 – 138Combined sources3
Beta strandi139 – 146Combined sources8
Helixi148 – 150Combined sources3
Beta strandi151 – 156Combined sources6
Beta strandi161 – 169Combined sources9
Beta strandi172 – 178Combined sources7
Beta strandi181 – 185Combined sources5
Helixi199 – 207Combined sources9
Beta strandi212 – 214Combined sources3
Helixi216 – 221Combined sources6
Helixi225 – 239Combined sources15
Turni241 – 243Combined sources3
Beta strandi250 – 254Combined sources5
Helixi255 – 257Combined sources3
Beta strandi262 – 272Combined sources11
Turni275 – 278Combined sources4
Beta strandi279 – 282Combined sources4
Helixi285 – 298Combined sources14
Beta strandi302 – 306Combined sources5
Beta strandi310 – 319Combined sources10
Beta strandi322 – 333Combined sources12
Beta strandi342 – 347Combined sources6
Helixi351 – 360Combined sources10
Helixi361 – 365Combined sources5
Beta strandi368 – 377Combined sources10
Beta strandi382 – 388Combined sources7
Helixi396 – 398Combined sources3
Helixi399 – 415Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PK8X-ray2.10A/B/C/D/E/F/G/H2-421[»]
1PX2X-ray2.23A/B2-421[»]
ProteinModelPortaliP09951.
SMRiP09951.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09951.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 28AAdd BLAST28
Regioni29 – 112B; linkerAdd BLAST84
Regioni113 – 420C; actin-binding and synaptic-vesicle bindingAdd BLAST308
Regioni421 – 655D; Pro-rich linkerAdd BLAST235
Regioni656 – 704EAdd BLAST49

Domaini

The A region binds phospholipids with a preference for negatively charged species.By similarity

Sequence similaritiesi

Belongs to the synapsin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3895. Eukaryota.
ENOG410XQH5. LUCA.
GeneTreeiENSGT00530000063319.
HOGENOMiHOG000231323.
HOVERGENiHBG016354.
InParanoidiP09951.
KOiK19941.
OMAiPIRQASQ.
OrthoDBiEOG091G0BZ1.
PhylomeDBiP09951.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
InterProiIPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR028713. SYN1.
IPR001359. Synapsin.
IPR020898. Synapsin_ATP-bd_dom.
IPR019735. Synapsin_CS.
IPR019736. Synapsin_P_site.
IPR020897. Synapsin_pre-ATP-grasp_dom.
[Graphical view]
PANTHERiPTHR10841. PTHR10841. 2 hits.
PTHR10841:SF22. PTHR10841:SF22. 2 hits.
PfamiPF02078. Synapsin. 1 hit.
PF02750. Synapsin_C. 1 hit.
PF10581. Synapsin_N. 1 hit.
[Graphical view]
PRINTSiPR01368. SYNAPSIN.
SUPFAMiSSF52440. SSF52440. 1 hit.
PROSITEiPS00415. SYNAPSIN_1. 1 hit.
PS00416. SYNAPSIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform IA (identifier: P09951-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNYLRRRLSD SNFMANLPNG YMTDLQRPQP PPPPPSAASP GATPGSAAAS
60 70 80 90 100
AERASTAAPV ASPAAPSPGS SGGGGFFSSL SNAVKQTTAA AAATFSEQVG
110 120 130 140 150
GGSGGAGRGG AAARVLLVID EPHTDWAKYF KGKKIHGEID IKVEQAEFSD
160 170 180 190 200
LNLVAHANGG FSVDMEVLRN GVKVVRSLKP DFVLIRQHAF SMARNGDYRS
210 220 230 240 250
LVIGLQYAGI PSVNSLHSVY NFCDKPWVFA QMVRLHKKLG TEEFPLIDQT
260 270 280 290 300
FYPNHKEMLS STTYPVVVKM GHAHSGMGKV KVDNQHDFQD IASVVALTKT
310 320 330 340 350
YATAEPFIDA KYDVRVQKIG QNYKAYMRTS VSGNWKTNTG SAMLEQIAMS
360 370 380 390 400
DRYKLWVDTC SEIFGGLDIC AVEALHGKDG RDHIIEVVGS SMPLIGDHQD
410 420 430 440 450
EDKQLIVELV VNKMTQALPR QRDASPGRGS HSQTPSPGAL PLGRQTSQQP
460 470 480 490 500
AGPPAQQRPP PQGGPPQPGP GPQRQGPPLQ QRPPPQGQQH LSGLGPPAGS
510 520 530 540 550
PLPQRLPSPT AAPQQSASQA TPMTQGQGRQ SRPVAGGPGA PPAARPPASP
560 570 580 590 600
SPQRQAGPPQ ATRQASISGP APPKVSGASP GGQQRQGPPQ KPPGPAGPIR
610 620 630 640 650
QASQAGPGPR TGPPTTQQPR PSGPGPAGRP TKPQLAQKPS QDVPPPIIAA
660 670 680 690 700
AGGPPHPQLN KSQSLTNAFN LPEPAPPRPS LSQDEVKAET IRSLRKSFAS

LFSD
Length:704
Mass (Da):73,988
Last modified:May 30, 2000 - v3
Checksum:i65799FEF7CFE18B5
GO
Isoform IB (identifier: P09951-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     660-668: NKSQSLTNA → KASPAQAQP
     669-704: Missing.

Show »
Length:668
Mass (Da):69,910
Checksum:iCE225AE8FCC92F8F
GO

Sequence cautioni

The sequence CAA28353 differs from that shown. Sequencing errors.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006318660 – 668NKSQSLTNA → KASPAQAQP in isoform IB. Curated9
Alternative sequenceiVSP_006319669 – 704Missing in isoform IB. CuratedAdd BLAST36

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27812 mRNA. Translation: AAA42145.1.
M27924 mRNA. Translation: AAA42148.1.
X04655 mRNA. Translation: CAA28353.1. Sequence problems.
PIRiA25704.
A30411.
RefSeqiNP_001104252.1. NM_001110782.2. [P09951-2]
NP_062006.1. NM_019133.2. [P09951-1]
UniGeneiRn.9923.

Genome annotation databases

EnsembliENSRNOT00000014250; ENSRNOP00000014250; ENSRNOG00000010365. [P09951-1]
GeneIDi24949.
KEGGirno:24949.
UCSCiRGD:3797. rat. [P09951-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27812 mRNA. Translation: AAA42145.1.
M27924 mRNA. Translation: AAA42148.1.
X04655 mRNA. Translation: CAA28353.1. Sequence problems.
PIRiA25704.
A30411.
RefSeqiNP_001104252.1. NM_001110782.2. [P09951-2]
NP_062006.1. NM_019133.2. [P09951-1]
UniGeneiRn.9923.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PK8X-ray2.10A/B/C/D/E/F/G/H2-421[»]
1PX2X-ray2.23A/B2-421[»]
ProteinModelPortaliP09951.
SMRiP09951.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247051. 1 interactor.
IntActiP09951. 5 interactors.
MINTiMINT-139278.
STRINGi10116.ENSRNOP00000014250.

PTM databases

iPTMnetiP09951.
PhosphoSitePlusiP09951.

Proteomic databases

PaxDbiP09951.
PRIDEiP09951.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000014250; ENSRNOP00000014250; ENSRNOG00000010365. [P09951-1]
GeneIDi24949.
KEGGirno:24949.
UCSCiRGD:3797. rat. [P09951-1]

Organism-specific databases

CTDi6853.
RGDi3797. Syn1.

Phylogenomic databases

eggNOGiKOG3895. Eukaryota.
ENOG410XQH5. LUCA.
GeneTreeiENSGT00530000063319.
HOGENOMiHOG000231323.
HOVERGENiHBG016354.
InParanoidiP09951.
KOiK19941.
OMAiPIRQASQ.
OrthoDBiEOG091G0BZ1.
PhylomeDBiP09951.

Miscellaneous databases

EvolutionaryTraceiP09951.
PROiP09951.

Gene expression databases

BgeeiENSRNOG00000010365.
GenevisibleiP09951. RN.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
InterProiIPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR028713. SYN1.
IPR001359. Synapsin.
IPR020898. Synapsin_ATP-bd_dom.
IPR019735. Synapsin_CS.
IPR019736. Synapsin_P_site.
IPR020897. Synapsin_pre-ATP-grasp_dom.
[Graphical view]
PANTHERiPTHR10841. PTHR10841. 2 hits.
PTHR10841:SF22. PTHR10841:SF22. 2 hits.
PfamiPF02078. Synapsin. 1 hit.
PF02750. Synapsin_C. 1 hit.
PF10581. Synapsin_N. 1 hit.
[Graphical view]
PRINTSiPR01368. SYNAPSIN.
SUPFAMiSSF52440. SSF52440. 1 hit.
PROSITEiPS00415. SYNAPSIN_1. 1 hit.
PS00416. SYNAPSIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYN1_RAT
AccessioniPrimary (citable) accession number: P09951
Secondary accession number(s): Q9WUX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: May 30, 2000
Last modified: November 2, 2016
This is version 149 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.