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P09950

- HEM1_YEAST

UniProt

P09950 - HEM1_YEAST

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Protein

5-aminolevulinate synthase, mitochondrial

Gene

HEM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

Cofactori

Pyridoxal phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei91 – 911SubstrateBy similarity
Binding sitei204 – 2041SubstrateBy similarity
Binding sitei223 – 2231SubstrateBy similarity
Binding sitei256 – 2561Pyridoxal phosphateBy similarity
Binding sitei284 – 2841Pyridoxal phosphateBy similarity
Binding sitei334 – 3341Pyridoxal phosphateBy similarity
Active sitei337 – 3371By similarity
Binding sitei366 – 3661Pyridoxal phosphateBy similarity
Binding sitei367 – 3671Pyridoxal phosphateBy similarity
Binding sitei452 – 4521SubstrateBy similarity

GO - Molecular functioni

  1. 5-aminolevulinate synthase activity Source: SGD
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. heme biosynthetic process Source: SGD
  2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Heme biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciYEAST:YDR232W-MONOMER.
UniPathwayiUPA00251; UER00375.

Names & Taxonomyi

Protein namesi
Recommended name:
5-aminolevulinate synthase, mitochondrial (EC:2.3.1.37)
Alternative name(s):
5-aminolevulinic acid synthase
Delta-ALA synthase
Delta-aminolevulinate synthase
Gene namesi
Name:HEM1
Synonyms:CYD1
Ordered Locus Names:YDR232W
ORF Names:YD9934.16
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDR232w.
SGDiS000002640. HEM1.

Subcellular locationi

Mitochondrion matrix 1 Publication

GO - Cellular componenti

  1. mitochondrial matrix Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 5485-aminolevulinate synthase, mitochondrialPRO_0000001245
Transit peptidei1 – ?Mitochondrion

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei337 – 3371N6-(pyridoxal phosphate)lysineCurated

Proteomic databases

MaxQBiP09950.
PaxDbiP09950.
PeptideAtlasiP09950.

Expressioni

Gene expression databases

GenevestigatoriP09950.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi32283. 39 interactions.
IntActiP09950. 4 interactions.
MINTiMINT-4481528.
STRINGi4932.YDR232W.

Structurei

3D structure databases

ProteinModelPortaliP09950.
SMRiP09950. Positions 72-481.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi26 – 6237Ala-richAdd
BLAST
Compositional biasi54 – 629Poly-Ala

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0156.
GeneTreeiENSGT00530000063111.
HOGENOMiHOG000221020.
InParanoidiP09950.
KOiK00643.
OMAiKLAQYPK.
OrthoDBiEOG7HHX1P.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09950-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQRSIFARFG NSSAAVSTLN RLSTTAAPHA KNGYATATGA GAAAATATAS
60 70 80 90 100
STHAAAAAAA AANHSTQESG FDYEGLIDSE LQKKRLDKSY RYFNNINRLA
110 120 130 140 150
KEFPLAHRQR EADKVTVWCS NDYLALSKHP EVLDAMHKTI DKYGCGAGGT
160 170 180 190 200
RNIAGHNIPT LNLEAELATL HKKEGALVFS SCYVANDAVL SLLGQKMKDL
210 220 230 240 250
VIFSDELNHA SMIVGIKHAN VKKHIFKHND LNELEQLLQS YPKSVPKLIA
260 270 280 290 300
FESVYSMAGS VADIEKICDL ADKYGALTFL DEVHAVGLYG PHGAGVAEHC
310 320 330 340 350
DFESHRASGI ATPKTNDKGG AKTVMDRVDM ITGTLGKSFG SVGGYVAASR
360 370 380 390 400
KLIDWFRSFA PGFIFTTTLP PSVMAGATAA IRYQRCHIDL RTSQQKHTMY
410 420 430 440 450
VKKAFHELGI PVIPNPSHIV PVLIGNADLA KQASDILINK HQIYVQAINF
460 470 480 490 500
PTVARGTERL RITPTPGHTN DLSDILINAV DDVFNELQLP RVRDWESQGG
510 520 530 540
LLGVGESGFV EESNLWTSSQ LSLTNDDLNP NVRDPIVKQL EVSSGIKQ
Length:548
Mass (Da):59,362
Last modified:July 1, 1989 - v1
Checksum:iFAAEDBAFCEDBE429
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti181 – 1811S → P in AAU09697. (PubMed:17322287)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M26329 Genomic DNA. Translation: AAA34668.1.
Z48612 Genomic DNA. Translation: CAA88511.1.
AY723780 Genomic DNA. Translation: AAU09697.1.
J03556 Genomic DNA. No translation available.
BK006938 Genomic DNA. Translation: DAA12073.1.
PIRiA24870. SYBYAL.
RefSeqiNP_010518.1. NM_001180540.1.

Genome annotation databases

EnsemblFungiiYDR232W; YDR232W; YDR232W.
GeneIDi851818.
KEGGisce:YDR232W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M26329 Genomic DNA. Translation: AAA34668.1 .
Z48612 Genomic DNA. Translation: CAA88511.1 .
AY723780 Genomic DNA. Translation: AAU09697.1 .
J03556 Genomic DNA. No translation available.
BK006938 Genomic DNA. Translation: DAA12073.1 .
PIRi A24870. SYBYAL.
RefSeqi NP_010518.1. NM_001180540.1.

3D structure databases

ProteinModelPortali P09950.
SMRi P09950. Positions 72-481.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32283. 39 interactions.
IntActi P09950. 4 interactions.
MINTi MINT-4481528.
STRINGi 4932.YDR232W.

Proteomic databases

MaxQBi P09950.
PaxDbi P09950.
PeptideAtlasi P09950.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDR232W ; YDR232W ; YDR232W .
GeneIDi 851818.
KEGGi sce:YDR232W.

Organism-specific databases

CYGDi YDR232w.
SGDi S000002640. HEM1.

Phylogenomic databases

eggNOGi COG0156.
GeneTreei ENSGT00530000063111.
HOGENOMi HOG000221020.
InParanoidi P09950.
KOi K00643.
OMAi KLAQYPK.
OrthoDBi EOG7HHX1P.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00375 .
BioCyci YEAST:YDR232W-MONOMER.

Miscellaneous databases

NextBioi 969683.
PROi P09950.

Gene expression databases

Genevestigatori P09950.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProi IPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view ]
Pfami PF00155. Aminotran_1_2. 1 hit.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 1 hit.
TIGRFAMsi TIGR01821. 5aminolev_synth. 1 hit.
PROSITEi PS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of the HEM1 gene and evidence for a precursor form of the mitochondrial 5-aminolevulinate synthase in Saccharomyces cerevisiae."
    Urban-Grimal D., Volland C., Garnier T., Dehoux P., Labbe-Bois R.
    Eur. J. Biochem. 156:511-519(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Constitutive expression of the yeast HEM1 gene is actually a composite of activation and repression."
    Keng T., Guarente L.
    Proc. Natl. Acad. Sci. U.S.A. 84:9113-9117(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-2.
  6. "The nine amino-terminal residues of delta-aminolevulinate synthase direct beta-galactosidase into the mitochondrial matrix."
    Keng T., Alani E., Guarente L.
    Mol. Cell. Biol. 6:355-364(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-75, SUBCELLULAR LOCATION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHEM1_YEAST
AccessioniPrimary (citable) accession number: P09950
Secondary accession number(s): D6VSL3, E9P946
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 29, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 22600 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3