Reviewed, UniProtKB/Swiss-Prot P09950 (HEM1_YEAST)

Names and origin

Protein names

Recommended name:
    5-aminolevulinate synthase, mitochondrial
    EC=2.3.1.37
Alternative name(s):
    5-aminolevulinic acid synthase
    Delta-aminolevulinate synthase
    Delta-ALA synthetase

Gene names

Name:	HEM1
Synonyms:	CYD1
Ordered Locus Names:	YDR232W
ORF Names:	YD9934.16

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.
Cofactor	Pyridoxal phosphate.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.
Subunit structure	Homodimer.
Subcellular location	Mitochondrion matrix. Ref.4
Miscellaneous	Present with 22600 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Heme biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Pyridoxal phosphate
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrial matrix Ref.4 Inferred from direct assay. Source: SGD
Molecular function	5-aminolevulinate synthase activity Inferred from direct assay. Source: SGD pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring nitrogenous groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion
	Chain	? – 548		5-aminolevulinate synthase, mitochondrial		PRO_0000001245
Regions
	Compositional bias	26 – 62	37	Ala-rich
	Compositional bias	54 – 62	9	Poly-Ala
Amino acid modifications
	Modified residue	337	1	N6-(pyridoxal phosphate)lysine Probable

Sequences

	Sequence		Length	Mass (Da)
	P09950-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: FAAEDBAFCEDBE429	FASTA	548	59,362
	10 20 30 40 50 60 MQRSIFARFG NSSAAVSTLN RLSTTAAPHA KNGYATATGA GAAAATATAS STHAAAAAAA 70 80 90 100 110 120 AANHSTQESG FDYEGLIDSE LQKKRLDKSY RYFNNINRLA KEFPLAHRQR EADKVTVWCS 130 140 150 160 170 180 NDYLALSKHP EVLDAMHKTI DKYGCGAGGT RNIAGHNIPT LNLEAELATL HKKEGALVFS 190 200 210 220 230 240 SCYVANDAVL SLLGQKMKDL VIFSDELNHA SMIVGIKHAN VKKHIFKHND LNELEQLLQS 250 260 270 280 290 300 YPKSVPKLIA FESVYSMAGS VADIEKICDL ADKYGALTFL DEVHAVGLYG PHGAGVAEHC 310 320 330 340 350 360 DFESHRASGI ATPKTNDKGG AKTVMDRVDM ITGTLGKSFG SVGGYVAASR KLIDWFRSFA 370 380 390 400 410 420 PGFIFTTTLP PSVMAGATAA IRYQRCHIDL RTSQQKHTMY VKKAFHELGI PVIPNPSHIV 430 440 450 460 470 480 PVLIGNADLA KQASDILINK HQIYVQAINF PTVARGTERL RITPTPGHTN DLSDILINAV 490 500 510 520 530 540 DDVFNELQLP RVRDWESQGG LLGVGESGFV EESNLWTSSQ LSLTNDDLNP NVRDPIVKQL EVSSGIKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of the HEM1 gene and evidence for a precursor form of the mitochondrial 5-aminolevulinate synthase in Saccharomyces cerevisiae." Urban-Grimal D., Volland C., Garnier T., Dehoux P., Labbe-Bois R. Eur. J. Biochem. 156:511-519(1986) [PubMed: 3516694] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Constitutive expression of the yeast HEM1 gene is actually a composite of activation and repression." Keng T., Guarente L. Proc. Natl. Acad. Sci. U.S.A. 84:9113-9117(1987) [PubMed: 3321068] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-2.
[4]	"The nine amino-terminal residues of delta-aminolevulinate synthase direct beta-galactosidase into the mitochondrial matrix." Keng T., Alani E., Guarente L. Mol. Cell. Biol. 6:355-364(1986) [PubMed: 3023841] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-75, SUBCELLULAR LOCATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J03556 Genomic DNA. No translation available. M26329 Genomic DNA. Translation: AAA34668.1. Z48612 Genomic DNA. Translation: CAA88511.1.
PIR	SYBYAL. A24870.
RefSeq	NP_010518.1.
3D structure databases
HSSP	HSSP built from PDB template 1FC4 based on UniProtKB P07912.
ModBase	Search...
Protein-protein interaction databases
STRING	P09950.
Proteomic databases
PeptideAtlas	P09950.
Genome annotation databases
Ensembl	YDR232W; YDR232W; YDR232W; Saccharomyces cerevisiae. [Genome view]
GeneID	851818.
GenomeReviews	Gene locus YDR232W in contig Z71256_GR.
KEGG	sce:YDR232W.
NMPDR	fig|4932.3.peg.1275.
Organism-specific databases
CYGD	YDR232w.
SGD	S000002640. HEM1.
Phylogenomic databases
HOGENOM	P09950.
OMA	FSPIKDI.
Enzyme and pathway databases
BRENDA	2.3.1.37. 250.
Gene expression databases
ArrayExpress	P09950.
Genevestigator	P09950.
GermOnline	YDR232W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR010961. 4pyrrol_synth_NH2levulA_synth. IPR001917. Aminotrans_II_pyridoxalP_BS. IPR004839. Aminotransferase_I/II. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01821. 5aminolev_synth. 1 hit.
PROSITE	PS00599. AA_TRANSFER_CLASS_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	969683.

Entry information

Entry name

HEM1_YEAST

Accession

Primary (citable) accession number: P09950

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	November 3, 2009
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)