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P09950

- HEM1_YEAST

UniProt

P09950 - HEM1_YEAST

Protein

5-aminolevulinate synthase, mitochondrial

Gene

HEM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

    Cofactori

    Pyridoxal phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei91 – 911SubstrateBy similarity
    Binding sitei204 – 2041SubstrateBy similarity
    Binding sitei223 – 2231SubstrateBy similarity
    Binding sitei256 – 2561Pyridoxal phosphateBy similarity
    Binding sitei284 – 2841Pyridoxal phosphateBy similarity
    Binding sitei334 – 3341Pyridoxal phosphateBy similarity
    Active sitei337 – 3371By similarity
    Binding sitei366 – 3661Pyridoxal phosphateBy similarity
    Binding sitei367 – 3671Pyridoxal phosphateBy similarity
    Binding sitei452 – 4521SubstrateBy similarity

    GO - Molecular functioni

    1. 5-aminolevulinate synthase activity Source: SGD
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. heme biosynthetic process Source: SGD
    2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Heme biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciYEAST:YDR232W-MONOMER.
    UniPathwayiUPA00251; UER00375.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-aminolevulinate synthase, mitochondrial (EC:2.3.1.37)
    Alternative name(s):
    5-aminolevulinic acid synthase
    Delta-ALA synthase
    Delta-aminolevulinate synthase
    Gene namesi
    Name:HEM1
    Synonyms:CYD1
    Ordered Locus Names:YDR232W
    ORF Names:YD9934.16
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR232w.
    SGDiS000002640. HEM1.

    Subcellular locationi

    Mitochondrion matrix 1 Publication

    GO - Cellular componenti

    1. mitochondrial matrix Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 5485-aminolevulinate synthase, mitochondrialPRO_0000001245
    Transit peptidei1 – ?Mitochondrion

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei337 – 3371N6-(pyridoxal phosphate)lysineCurated

    Proteomic databases

    MaxQBiP09950.
    PaxDbiP09950.
    PeptideAtlasiP09950.

    Expressioni

    Gene expression databases

    GenevestigatoriP09950.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    BioGridi32283. 39 interactions.
    IntActiP09950. 4 interactions.
    MINTiMINT-4481528.
    STRINGi4932.YDR232W.

    Structurei

    3D structure databases

    ProteinModelPortaliP09950.
    SMRiP09950. Positions 72-481.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi26 – 6237Ala-richAdd
    BLAST
    Compositional biasi54 – 629Poly-Ala

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0156.
    GeneTreeiENSGT00530000063111.
    HOGENOMiHOG000221020.
    KOiK00643.
    OMAiKLAQYPK.
    OrthoDBiEOG7HHX1P.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
    IPR001917. Aminotrans_II_pyridoxalP_BS.
    IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
    PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09950-1 [UniParc]FASTAAdd to Basket

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    MQRSIFARFG NSSAAVSTLN RLSTTAAPHA KNGYATATGA GAAAATATAS    50
    STHAAAAAAA AANHSTQESG FDYEGLIDSE LQKKRLDKSY RYFNNINRLA 100
    KEFPLAHRQR EADKVTVWCS NDYLALSKHP EVLDAMHKTI DKYGCGAGGT 150
    RNIAGHNIPT LNLEAELATL HKKEGALVFS SCYVANDAVL SLLGQKMKDL 200
    VIFSDELNHA SMIVGIKHAN VKKHIFKHND LNELEQLLQS YPKSVPKLIA 250
    FESVYSMAGS VADIEKICDL ADKYGALTFL DEVHAVGLYG PHGAGVAEHC 300
    DFESHRASGI ATPKTNDKGG AKTVMDRVDM ITGTLGKSFG SVGGYVAASR 350
    KLIDWFRSFA PGFIFTTTLP PSVMAGATAA IRYQRCHIDL RTSQQKHTMY 400
    VKKAFHELGI PVIPNPSHIV PVLIGNADLA KQASDILINK HQIYVQAINF 450
    PTVARGTERL RITPTPGHTN DLSDILINAV DDVFNELQLP RVRDWESQGG 500
    LLGVGESGFV EESNLWTSSQ LSLTNDDLNP NVRDPIVKQL EVSSGIKQ 548
    Length:548
    Mass (Da):59,362
    Last modified:July 1, 1989 - v1
    Checksum:iFAAEDBAFCEDBE429
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti181 – 1811S → P in AAU09697. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M26329 Genomic DNA. Translation: AAA34668.1.
    Z48612 Genomic DNA. Translation: CAA88511.1.
    AY723780 Genomic DNA. Translation: AAU09697.1.
    J03556 Genomic DNA. No translation available.
    BK006938 Genomic DNA. Translation: DAA12073.1.
    PIRiA24870. SYBYAL.
    RefSeqiNP_010518.1. NM_001180540.1.

    Genome annotation databases

    EnsemblFungiiYDR232W; YDR232W; YDR232W.
    GeneIDi851818.
    KEGGisce:YDR232W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M26329 Genomic DNA. Translation: AAA34668.1 .
    Z48612 Genomic DNA. Translation: CAA88511.1 .
    AY723780 Genomic DNA. Translation: AAU09697.1 .
    J03556 Genomic DNA. No translation available.
    BK006938 Genomic DNA. Translation: DAA12073.1 .
    PIRi A24870. SYBYAL.
    RefSeqi NP_010518.1. NM_001180540.1.

    3D structure databases

    ProteinModelPortali P09950.
    SMRi P09950. Positions 72-481.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32283. 39 interactions.
    IntActi P09950. 4 interactions.
    MINTi MINT-4481528.
    STRINGi 4932.YDR232W.

    Proteomic databases

    MaxQBi P09950.
    PaxDbi P09950.
    PeptideAtlasi P09950.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR232W ; YDR232W ; YDR232W .
    GeneIDi 851818.
    KEGGi sce:YDR232W.

    Organism-specific databases

    CYGDi YDR232w.
    SGDi S000002640. HEM1.

    Phylogenomic databases

    eggNOGi COG0156.
    GeneTreei ENSGT00530000063111.
    HOGENOMi HOG000221020.
    KOi K00643.
    OMAi KLAQYPK.
    OrthoDBi EOG7HHX1P.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00375 .
    BioCyci YEAST:YDR232W-MONOMER.

    Miscellaneous databases

    NextBioi 969683.
    PROi P09950.

    Gene expression databases

    Genevestigatori P09950.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR010961. 4pyrrol_synth_NH2levulA_synth.
    IPR001917. Aminotrans_II_pyridoxalP_BS.
    IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00155. Aminotran_1_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    TIGRFAMsi TIGR01821. 5aminolev_synth. 1 hit.
    PROSITEi PS00599. AA_TRANSFER_CLASS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of the HEM1 gene and evidence for a precursor form of the mitochondrial 5-aminolevulinate synthase in Saccharomyces cerevisiae."
      Urban-Grimal D., Volland C., Garnier T., Dehoux P., Labbe-Bois R.
      Eur. J. Biochem. 156:511-519(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Constitutive expression of the yeast HEM1 gene is actually a composite of activation and repression."
      Keng T., Guarente L.
      Proc. Natl. Acad. Sci. U.S.A. 84:9113-9117(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-2.
    6. "The nine amino-terminal residues of delta-aminolevulinate synthase direct beta-galactosidase into the mitochondrial matrix."
      Keng T., Alani E., Guarente L.
      Mol. Cell. Biol. 6:355-364(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-75, SUBCELLULAR LOCATION.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiHEM1_YEAST
    AccessioniPrimary (citable) accession number: P09950
    Secondary accession number(s): D6VSL3, E9P946
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 136 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 22600 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3