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Protein

Citrate synthase

Gene

gltA

Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

Enzyme regulationi

Allosterically inhibited by NADH.

Pathway:itricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes isocitrate from oxaloacetate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase (gltA)
  2. Aconitate hydratase A (acnA)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei311 – 3111PROSITE-ProRule annotation
Active sitei370 – 3701PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase (EC:2.3.3.16)
Gene namesi
Name:gltA
Ordered Locus Names:RP844
OrganismiRickettsia prowazekii (strain Madrid E)
Taxonomic identifieri272947 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
ProteomesiUP000002480 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 436436Citrate synthasePRO_0000169967Add
BLAST

Proteomic databases

PRIDEiP09948.

Interactioni

Subunit structurei

Homohexamer.

Protein-protein interaction databases

STRINGi272947.RP844.

Structurei

3D structure databases

ProteinModelPortaliP09948.
SMRiP09948. Positions 12-435.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.Curated

Phylogenomic databases

eggNOGiCOG0372.
HOGENOMiHOG000021224.
KOiK01647.
OMAiNKEDGVR.
OrthoDBiEOG6P8TP4.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
IPR010953. Citrate_synthase_typ-I.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001369. Citrate_synth. 1 hit.
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01798. cit_synth_I. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09948-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNGNNNNLE FAELKIRGKL FKLPILKASI GKDVIDISRV SAEADYFTYD
60 70 80 90 100
PGFMSTASCQ STITYIDGDK GILWYRGYDI KDLAEKSDFL EVAYLMIYGE
110 120 130 140 150
LPSSDQYCNF TKKVAHHSLV NERLHYLFQT FCSSSHPMAI MLAAVGSLSA
160 170 180 190 200
FYPDLLNFNE TDYELTAIRM IAKIPTIAAM SYKYSIGQPF IYPDNSLDFT
210 220 230 240 250
ENFLHMMFAT PCTKYKVNPI IKNALNKIFI LHADHEQNAS TSTVRIAGSS
260 270 280 290 300
GANPFACIST GIASLWGPAH GGANEAVINM LKEIGSSENI PKYVAKAKDK
310 320 330 340 350
NDPFRLMGFG HRVYKSYDPR AAVLKETCKE VLNELGQLDN NPLLQIAIEL
360 370 380 390 400
EALALKDEYF IERKLYPNVD FYSGIIYKAM GIPSQMFTVL FAIARTVGWM
410 420 430
AQWKEMHEDP EQKISRPRQL YTGYVHREYK CIVERK
Length:436
Mass (Da):49,324
Last modified:July 1, 1989 - v1
Checksum:i57F947C151A494B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17149 Genomic DNA. Translation: AAA26383.1.
AJ235273 Genomic DNA. Translation: CAA15268.1.
U14334 Genomic DNA. Translation: AAA85524.1.
U20244 Genomic DNA. Translation: AAA85708.1.
PIRiA26971. YKRECP.
RefSeqiNP_221192.1. NC_000963.1.
WP_004596795.1. NC_000963.1.

Genome annotation databases

EnsemblBacteriaiCAA15268; CAA15268; CAA15268.
GeneIDi883416.
KEGGirpr:RP844.
PATRICi17902564. VBIRicPro72556_0882.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17149 Genomic DNA. Translation: AAA26383.1.
AJ235273 Genomic DNA. Translation: CAA15268.1.
U14334 Genomic DNA. Translation: AAA85524.1.
U20244 Genomic DNA. Translation: AAA85708.1.
PIRiA26971. YKRECP.
RefSeqiNP_221192.1. NC_000963.1.
WP_004596795.1. NC_000963.1.

3D structure databases

ProteinModelPortaliP09948.
SMRiP09948. Positions 12-435.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272947.RP844.

Proteomic databases

PRIDEiP09948.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAA15268; CAA15268; CAA15268.
GeneIDi883416.
KEGGirpr:RP844.
PATRICi17902564. VBIRicPro72556_0882.

Phylogenomic databases

eggNOGiCOG0372.
HOGENOMiHOG000021224.
KOiK01647.
OMAiNKEDGVR.
OrthoDBiEOG6P8TP4.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
IPR010953. Citrate_synthase_typ-I.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001369. Citrate_synth. 1 hit.
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01798. cit_synth_I. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the Rickettsia prowazekii citrate synthase gene."
    Wood D.O., Williamson L.R., Winkler H.H., Krause D.C.
    J. Bacteriol. 169:3564-3572(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Madrid E.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Madrid E.
  3. "The citrate synthase-encoding gene of Rickettsia prowazekii is controlled by two promoters."
    Cai J., Pang H., Wood D.O., Winkler H.H.
    Gene 163:115-119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-35.
    Strain: Madrid E.
  4. Balayeva N., Eremeeva M., Tissot-Dupont H., Zakharov I., Raoult D.
    Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 267-392.
    Strain: ATCC VR-142 / Breinl.

Entry informationi

Entry nameiCISY_RICPR
AccessioniPrimary (citable) accession number: P09948
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: July 22, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Rickettsia prowazekii
    Rickettsia prowazekii (strain Madrid E): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.