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P09945

- HIRV2_HIRME

UniProt

P09945 - HIRV2_HIRME

Protein

Hirudin variant-2

Gene
N/A
Organism
Hirudo medicinalis (Medicinal leech)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Hirudin is a potent thrombin-specific protease inhibitor. It forms a stable non-covalent complex with alpha-thrombin, thereby abolishing its ability to cleave fibrinogen.

    GO - Molecular functioni

    1. serine-type endopeptidase inhibitor activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Protease inhibitor, Serine protease inhibitor

    Protein family/group databases

    MEROPSiI14.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hirudin variant-2
    OrganismiHirudo medicinalis (Medicinal leech)
    Taxonomic identifieri6421 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaLophotrochozoaAnnelidaClitellataHirudinidaHirudineaArhynchobdellidaHirudiniformesHirudinidaeHirudo

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Protein family/group databases

    Allergomei9843. Hir me Hirudin.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei‹1 – 7›7
    Chaini8 – 7265Hirudin variant-2PRO_0000013441Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi13 ↔ 211 Publication
    Disulfide bondi23 ↔ 351 Publication
    Disulfide bondi29 ↔ 461 Publication
    Glycosylationi52 – 521O-linked (GalNAc...)By similarity
    Modified residuei70 – 701Sulfotyrosine1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Sulfation

    Structurei

    Secondary structure

    1
    72
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi18 – 225
    Beta strandi24 – 263
    Beta strandi33 – 364
    Beta strandi45 – 495
    Helixi68 – 703

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1A2CX-ray2.10I60-71[»]
    1A46X-ray2.12I60-71[»]
    1A4WX-ray1.80I60-71[»]
    1A5GX-ray2.06I60-71[»]
    1A61X-ray2.20I60-71[»]
    1B5GX-ray2.07I60-71[»]
    1DOJX-ray1.70B62-72[»]
    1K21X-ray1.86I60-71[»]
    1K22X-ray1.93I60-71[»]
    1NRSX-ray2.40I60-71[»]
    1TMBX-ray2.30I60-71[»]
    1TMUX-ray2.50J62-72[»]
    1VZQX-ray1.54I62-72[»]
    1W7GX-ray1.65I60-71[»]
    1WAYX-ray2.02I62-71[»]
    2C8WX-ray1.96I61-72[»]
    2C8XX-ray2.17I61-72[»]
    2C8YX-ray2.20I61-72[»]
    2C8ZX-ray2.14I61-72[»]
    2C90X-ray2.25I61-72[»]
    2C93X-ray2.20I61-72[»]
    2R2MX-ray2.10H62-72[»]
    2ZFQX-ray1.80I60-71[»]
    2ZFRX-ray1.85I60-71[»]
    2ZG0X-ray1.75I60-71[»]
    2ZHEX-ray2.10I60-71[»]
    2ZHFX-ray1.98I60-71[»]
    2ZHWX-ray2.02I60-71[»]
    3BIUX-ray2.30I60-71[»]
    3BIVX-ray1.80I60-71[»]
    3F68X-ray1.75I61-71[»]
    3HATX-ray2.50I60-71[»]
    3HTCX-ray2.30I8-72[»]
    3P17X-ray1.43I60-71[»]
    3QTOX-ray1.52I60-72[»]
    3QTVX-ray1.63I60-72[»]
    3QWCX-ray1.75I60-72[»]
    3QX5X-ray1.35I60-72[»]
    3RLWX-ray1.69I60-72[»]
    3RLYX-ray1.51I60-72[»]
    3RM0X-ray1.34I60-72[»]
    3RM2X-ray1.23I60-72[»]
    3RMLX-ray1.53I60-72[»]
    3RMMX-ray1.58I60-72[»]
    3RMNX-ray1.78I60-72[»]
    3RMOX-ray1.40I60-72[»]
    3SHAX-ray1.52I60-72[»]
    3SHCX-ray1.90I60-72[»]
    3SI3X-ray1.55I60-72[»]
    3SI4X-ray1.27I60-72[»]
    3SV2X-ray1.30I60-72[»]
    3T5FX-ray1.45I60-72[»]
    3TU7X-ray2.49I61-72[»]
    3U98X-ray1.45I60-72[»]
    3U9AX-ray1.58I60-72[»]
    3UWJX-ray1.50I60-72[»]
    4BAOX-ray1.87D62-71[»]
    4E7RX-ray2.25I/J62-72[»]
    4HTCX-ray2.30I8-72[»]
    4LOYX-ray1.77I62-71[»]
    4N3LX-ray1.94I60-71[»]
    4NZEX-ray1.98I60-71[»]
    ProteinModelPortaliP09945.
    SMRiP09945. Positions 8-72.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP09945.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni8 – 103Interaction with thrombin active site
    Regioni62 – 7211Interaction with fibrinogen-binding exosite of thrombinAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.70.10.10. 1 hit.
    InterProiIPR024793. Hirudin.
    IPR011061. Hirudin/antistatin.
    IPR000429. Prot_inh_hirudin.
    [Graphical view]
    PfamiPF00713. Hirudin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001640. Hirudin. 1 hit.
    PRINTSiPR00777. HIRUDIN.
    ProDomiPD004216. Prot_inh_hirudin. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF57262. SSF57262. 1 hit.

    Sequencei

    Sequence statusi: Fragment.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09945-1 [UniParc]FASTAAdd to Basket

    « Hide

    AICVSQAITY TDCTESGQNL CLCEGSNVCG KGNKCILGSN GKGNQCVTGE   50
    GTPNPESHNN GDFEEIPEEY LQ 72
    Length:72
    Mass (Da):7,571
    Last modified:July 1, 1989 - v1
    Checksum:iC6EF2F28E9C45A5B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M12693 mRNA. Translation: AAA29195.1.
    PIRiA37417.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M12693 mRNA. Translation: AAA29195.1 .
    PIRi A37417.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1A2C X-ray 2.10 I 60-71 [» ]
    1A46 X-ray 2.12 I 60-71 [» ]
    1A4W X-ray 1.80 I 60-71 [» ]
    1A5G X-ray 2.06 I 60-71 [» ]
    1A61 X-ray 2.20 I 60-71 [» ]
    1B5G X-ray 2.07 I 60-71 [» ]
    1DOJ X-ray 1.70 B 62-72 [» ]
    1K21 X-ray 1.86 I 60-71 [» ]
    1K22 X-ray 1.93 I 60-71 [» ]
    1NRS X-ray 2.40 I 60-71 [» ]
    1TMB X-ray 2.30 I 60-71 [» ]
    1TMU X-ray 2.50 J 62-72 [» ]
    1VZQ X-ray 1.54 I 62-72 [» ]
    1W7G X-ray 1.65 I 60-71 [» ]
    1WAY X-ray 2.02 I 62-71 [» ]
    2C8W X-ray 1.96 I 61-72 [» ]
    2C8X X-ray 2.17 I 61-72 [» ]
    2C8Y X-ray 2.20 I 61-72 [» ]
    2C8Z X-ray 2.14 I 61-72 [» ]
    2C90 X-ray 2.25 I 61-72 [» ]
    2C93 X-ray 2.20 I 61-72 [» ]
    2R2M X-ray 2.10 H 62-72 [» ]
    2ZFQ X-ray 1.80 I 60-71 [» ]
    2ZFR X-ray 1.85 I 60-71 [» ]
    2ZG0 X-ray 1.75 I 60-71 [» ]
    2ZHE X-ray 2.10 I 60-71 [» ]
    2ZHF X-ray 1.98 I 60-71 [» ]
    2ZHW X-ray 2.02 I 60-71 [» ]
    3BIU X-ray 2.30 I 60-71 [» ]
    3BIV X-ray 1.80 I 60-71 [» ]
    3F68 X-ray 1.75 I 61-71 [» ]
    3HAT X-ray 2.50 I 60-71 [» ]
    3HTC X-ray 2.30 I 8-72 [» ]
    3P17 X-ray 1.43 I 60-71 [» ]
    3QTO X-ray 1.52 I 60-72 [» ]
    3QTV X-ray 1.63 I 60-72 [» ]
    3QWC X-ray 1.75 I 60-72 [» ]
    3QX5 X-ray 1.35 I 60-72 [» ]
    3RLW X-ray 1.69 I 60-72 [» ]
    3RLY X-ray 1.51 I 60-72 [» ]
    3RM0 X-ray 1.34 I 60-72 [» ]
    3RM2 X-ray 1.23 I 60-72 [» ]
    3RML X-ray 1.53 I 60-72 [» ]
    3RMM X-ray 1.58 I 60-72 [» ]
    3RMN X-ray 1.78 I 60-72 [» ]
    3RMO X-ray 1.40 I 60-72 [» ]
    3SHA X-ray 1.52 I 60-72 [» ]
    3SHC X-ray 1.90 I 60-72 [» ]
    3SI3 X-ray 1.55 I 60-72 [» ]
    3SI4 X-ray 1.27 I 60-72 [» ]
    3SV2 X-ray 1.30 I 60-72 [» ]
    3T5F X-ray 1.45 I 60-72 [» ]
    3TU7 X-ray 2.49 I 61-72 [» ]
    3U98 X-ray 1.45 I 60-72 [» ]
    3U9A X-ray 1.58 I 60-72 [» ]
    3UWJ X-ray 1.50 I 60-72 [» ]
    4BAO X-ray 1.87 D 62-71 [» ]
    4E7R X-ray 2.25 I/J 62-72 [» ]
    4HTC X-ray 2.30 I 8-72 [» ]
    4LOY X-ray 1.77 I 62-71 [» ]
    4N3L X-ray 1.94 I 60-71 [» ]
    4NZE X-ray 1.98 I 60-71 [» ]
    ProteinModelPortali P09945.
    SMRi P09945. Positions 8-72.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    Allergomei 9843. Hir me Hirudin.
    MEROPSi I14.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P09945.

    Family and domain databases

    Gene3Di 2.70.10.10. 1 hit.
    InterProi IPR024793. Hirudin.
    IPR011061. Hirudin/antistatin.
    IPR000429. Prot_inh_hirudin.
    [Graphical view ]
    Pfami PF00713. Hirudin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001640. Hirudin. 1 hit.
    PRINTSi PR00777. HIRUDIN.
    ProDomi PD004216. Prot_inh_hirudin. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF57262. SSF57262. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of a cDNA coding for the anticoagulant hirudin from the bloodsucking leech, Hirudo medicinalis."
      Harvey R.P., Degryse E., Stefani L., Schamber F., Cazenave J.-P., Courtney M., Tolstoshev P., Lecocq J.-P.
      Proc. Natl. Acad. Sci. U.S.A. 83:1084-1088(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SULFATION AT TYR-70.
    2. "The structure of a complex of recombinant hirudin and human alpha-thrombin."
      Rydel T.J., Ravichandran K.G., Tulinsky A., Bode W., Huber R., Roitsch C., Fenton J.W. II
      Science 249:277-280(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 8-72 OF MUTANT LYS-54 IN COMPLEX WITH THROMBIN, DISULFIDE BONDS.
    3. "Refined structure of the hirudin-thrombin complex."
      Rydel T.J., Tulinsky A., Bode W., Huber R.
      J. Mol. Biol. 221:583-601(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 8-72 IN COMPLEX WITH THROMBIN.

    Entry informationi

    Entry nameiHIRV2_HIRME
    AccessioniPrimary (citable) accession number: P09945
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3