ID   HIRPA_HIRME             Reviewed;          66 AA.
AC   P09944;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   16-JUN-2009, entry version 61.
DE   RecName: Full=Hirudin-PA;
OS   Hirudo medicinalis (Medicinal leech).
OC   Eukaryota; Metazoa; Annelida; Clitellata; Hirudinida; Hirudinea;
OC   Arhynchobdellida; Hirudiniformes; Hirudinidae; Hirudo.
OX   NCBI_TaxID=6421;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   MEDLINE=87026247; PubMed=3768144;
RA   Dodt J., Machleidt W., Seemueller U., Maschler R., Fritz H.;
RT   "Isolation and characterization of hirudin isoinhibitors and sequence
RT   analysis of hirudin PA.";
RL   Biol. Chem. Hoppe-Seyler 367:803-811(1986).
CC   -!- FUNCTION: Hirudin is a potent thrombin-specific protease
CC       inhibitor. It forms a stable non-covalent complex with alpha-
CC       thrombin, thereby abolishing its ability to cleave fibrinogen.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I14 (hirudin)
CC       family.
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DR   PIR; A24350; A24350.
DR   SMR; P09944; 1-62.
DR   MEROPS; I14.001; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   InterPro; IPR000429; Prot_inh_hirudin.
DR   Gene3D; G3DSA:2.70.10.10; Prot_inh_hirudin; 1.
DR   Pfam; PF00713; Hirudin; 1.
DR   PIRSF; PIRSF001640; Hirudin; 1.
DR   PRINTS; PR00777; HIRUDIN.
DR   ProDom; PD004216; Prot_inh_hirudin; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Glycoprotein;
KW   Protease inhibitor; Secreted; Serine protease inhibitor; Sulfation.
FT   CHAIN         1     66       Hirudin-PA.
FT                                /FTId=PRO_0000195640.
FT   REGION        1      3       Interaction with thrombin active site (By
FT                                similarity).
FT   REGION       55     66       Interaction with fibrinogen-binding
FT                                exosite of thrombin (By similarity).
FT   MOD_RES      64     64       Sulfotyrosine.
FT   CARBOHYD     45     45       O-linked (GalNAc...) (By similarity).
FT   DISULFID      6     14       By similarity.
FT   DISULFID     16     28       By similarity.
FT   DISULFID     22     39       By similarity.
SQ   SEQUENCE   66 AA;  7026 MW;  FA1B80B7F4FEA317 CRC64;
     ITYTDCTESG QNLCLCEGSN VCGKGNKCIL GSQGKDNQCV TGEGTPKPQS HNQGDFEPIP
     EDAYDE
//