P09944 (HIRPA_HIRME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Hirudin-PA
Organism	Hirudo medicinalis (Medicinal leech)
Taxonomic identifier	6421 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Annelida › Clitellata › Hirudinida › Hirudinea › Arhynchobdellida › Hirudiniformes › Hirudinidae › Hirudo

Protein attributes

Sequence length	66 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hirudin is a potent thrombin-specific protease inhibitor. It forms a stable non-covalent complex with alpha-thrombin, thereby abolishing its ability to cleave fibrinogen.
Subcellular location	Secreted.
Sequence similarities	Belongs to the protease inhibitor I14 (hirudin) family. [View classification]

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Disulfide bond Glycoprotein Sulfation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 66	66	Hirudin-PA		PRO_0000195640
Regions
	Region	1 – 3	3	Interaction with thrombin active site By similarity
	Region	55 – 66	12	Interaction with fibrinogen-binding exosite of thrombin By similarity
Amino acid modifications
	Modified residue	64	1	Sulfotyrosine
	Glycosylation	45	1	O-linked (GalNAc...) By similarity
	Disulfide bond	6 ↔ 14		By similarity
	Disulfide bond	16 ↔ 28		By similarity
	Disulfide bond	22 ↔ 39		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P09944 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: FA1B80B7F4FEA317	FASTA	66	7,026
	10 20 30 40 50 60 ITYTDCTESG QNLCLCEGSN VCGKGNKCIL GSQGKDNQCV TGEGTPKPQS HNQGDFEPIP EDAYDE

References

[1]	"Isolation and characterization of hirudin isoinhibitors and sequence analysis of hirudin PA." Dodt J., Machleidt W., Seemueller U., Maschler R., Fritz H. Biol. Chem. Hoppe-Seyler 367:803-811(1986) [PubMed: 3768144] [Abstract] Cited for: PROTEIN SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
PIR	A24350.
3D structure databases
ProteinModelPortal	P09944.
SMR	P09944. Positions 1-62.
ModBase	Search...
Protein family/group databases
MEROPS	I14.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000429. Prot_inh_hirudin. IPR011061. Prot_inh_I14/15_hirudin/antisn. [Graphical view]
Pfam	PF00713. Hirudin. 1 hit. [Graphical view]
PIRSF	PIRSF001640. Hirudin. 1 hit.
PRINTS	PR00777. HIRUDIN.
ProDom	PD004216. Prot_inh_hirudin. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF57262. Antihaemostatic. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HIRPA_HIRME

Accession

Primary (citable) accession number: P09944

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	December 14, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)