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P09933

- PERT_PIG

UniProt

P09933 - PERT_PIG

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Protein
Thyroid peroxidase
Gene
TPO
Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T3 and T4.

Catalytic activityi

2 iodide + H2O2 + 2 H+ = 2 iodine + 2 H2O.
[Thyroglobulin]-L-tyrosine + iodide + H2O2 = [thyroglobulin]-3-iodo-L-tyrosine + 2 H2O.
[Thyroglobulin]-3-iodo-L-tyrosine + iodide + H2O2 = [thyroglobulin]-3,5-diiodo-L-tyrosine + 2 H2O.
2 [thyroglobulin]-3,5-diiodo-L-tyrosine + H2O2 = [thyroglobulin]-L-thyroxine + [thyroglobulin]-aminoacrylate + 2 H2O.
[Thyroglobulin]-3-iodo-L-tyrosine + [thyroglobulin]-3,5-diiodo-L-tyrosine + H2O2 = [thyroglobulin]-3,5,3'-triiodo-L-thyronine + [thyroglobulin]-aminoacrylate + 2 H2O.

Cofactori

Binds 1 calcium ion per heterodimer By similarity.
Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei238 – 2381Heme (covalent; via 2 links) By similarity
Active sitei239 – 2391Proton acceptor By similarity
Metal bindingi240 – 2401Calcium By similarity
Metal bindingi321 – 3211Calcium By similarity
Metal bindingi323 – 3231Calcium; via carbonyl oxygen By similarity
Metal bindingi325 – 3251Calcium By similarity
Metal bindingi327 – 3271Calcium By similarity
Sitei395 – 3951Transition state stabilizer By similarity
Binding sitei398 – 3981Heme (covalent; via 2 links) By similarity
Metal bindingi493 – 4931Iron (heme axial ligand) By similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. heme binding Source: InterPro
  3. iodide peroxidase activity Source: UniProtKB-EC

GO - Biological processi

  1. hormone biosynthetic process Source: UniProtKB-KW
  2. hydrogen peroxide catabolic process Source: UniProtKB-KW
  3. thyroid hormone generation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide, Thyroid hormones biosynthesis

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14809.
UniPathwayiUPA00194.

Protein family/group databases

PeroxiBasei3329. SscTPO.

Names & Taxonomyi

Protein namesi
Recommended name:
Thyroid peroxidase (EC:1.11.1.8)
Short name:
TPO
Gene namesi
Name:TPO
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini19 – 844826Extracellular Reviewed prediction
Add
BLAST
Transmembranei845 – 86925Helical; Reviewed prediction
Add
BLAST
Topological domaini870 – 92657Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1414 Reviewed prediction
Add
BLAST
Chaini15 – 926912Thyroid peroxidase
PRO_0000023664Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi129 – 1291N-linked (GlcNAc...)1 Publication
Disulfide bondi142 ↔ 158 By similarity
Disulfide bondi259 ↔ 269 By similarity
Disulfide bondi263 ↔ 286 By similarity
Glycosylationi277 – 2771N-linked (GlcNAc...)1 Publication
Glycosylationi307 – 3071N-linked (GlcNAc...)1 Publication
Glycosylationi342 – 3421N-linked (GlcNAc...)1 Publication
Disulfide bondi596 ↔ 653 By similarity
Disulfide bondi694 ↔ 719 By similarity
Disulfide bondi740 ↔ 780 By similarity
Disulfide bondi766 ↔ 792 By similarity
Disulfide bondi798 ↔ 812 By similarity
Disulfide bondi806 ↔ 821 By similarity
Disulfide bondi823 ↔ 836 By similarity

Post-translational modificationi

Heme is covalently bound through a H2O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface By similarity.
Cleaved in its N-terminal part By similarity.
N-glycosylated; contains mannose and N-acetylglucosamine.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Interacts with DUOX1, DUOX2 and CYBA By similarity.

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000009228.

Structurei

3D structure databases

ProteinModelPortaliP09933.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini738 – 79356Sushi
Add
BLAST
Domaini794 – 83744EGF-like; calcium-binding Reviewed prediction
Add
BLAST

Sequence similaritiesi

Contains 1 EGF-like domain.

Keywords - Domaini

EGF-like domain, Signal, Sushi, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG262194.
HOGENOMiHOG000016084.
HOVERGENiHBG000071.

Family and domain databases

Gene3Di1.10.640.10. 1 hit.
InterProiIPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR010255. Haem_peroxidase.
IPR019791. Haem_peroxidase_animal.
IPR000436. Sushi_SCR_CCP.
IPR029589. TPO.
[Graphical view]
PANTHERiPTHR11475:SF60. PTHR11475:SF60. 1 hit.
PfamiPF03098. An_peroxidase. 1 hit.
PF07645. EGF_CA. 1 hit.
PF00084. Sushi. 1 hit.
[Graphical view]
PRINTSiPR00457. ANPEROXIDASE.
SMARTiSM00032. CCP. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view]
SUPFAMiSSF48113. SSF48113. 1 hit.
SSF57535. SSF57535. 1 hit.
PROSITEiPS50026. EGF_3. 1 hit.
PS00435. PEROXIDASE_1. 1 hit.
PS50292. PEROXIDASE_3. 1 hit.
PS50923. SUSHI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09933-1 [UniParc]FASTAAdd to Basket

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MGARAVLGVT LAVACAGAFF ASILRRKDLL GGDTEASGVA GLVEASRLLV    50
DEAIHTTMRR NLRKRGIFSP SQLLSFSKLP EPTSRTASRA AEIMETAVQE 100
VKRRVCRRRD TDQLPTDVLS EELLSTIANL SGCLPHMLPP SCPHTCLANK 150
YRLITGACNN RDHPRWGASN TALARWLPPA YEDGVTEPRG WNPHFLYNGL 200
PLPPVREVTR QVIHVSNEAV TEDGQYSDLL MAWGQYIDHD IAFTPQSTSK 250
AAFAGGADCQ LTCENRSPCF PIQLPTNASG AAGATCLPFY RSSAACGSGR 300
QGALVGNLSW AAPRQQMNGL TSFLDASTVY GSSPAQEQRL RNWTSAEGLL 350
RVNTRHRDAG RAFLPFAPPP APPACAPEPG TPAARAPCFL AGDSRASEVP 400
GLTALHTLWL REHNRLAAAF KALNAHWSAD TVYQEARKVV GALHQIVTLR 450
DYVPKILGAE AFGQHVGPYQ GYDPAVDPTV SNVFSTAAFR FGHATIHPLV 500
RRLDARFQEH PGSHLPLRAA FFQPWRLLRE GGVDPVLRGL LARPAKLQVQ 550
DQLMNEELTE RLFVLSNSGT LDLASINLQR GRDHGLPGYN EWREFCGLSR 600
LETWADLSAA TANGRVADRI LGLYQHPDNI DVWLGGLAES FLPGARTGPL 650
FACIIGKQMR ALRDGDRFWW ENPGVFTEAQ RRELSRHSMS RVICDNSGLS 700
HVPLDAFRVG QWPQEFEPCA SIQGMDLGAW REAPPSGDAC GFPDPVEDGG 750
FLLCEERGQR VLVFSCRHGF RLRGPAQITC TPRGWDSPPP LCKDINECED 800
ETDPPCHASA RCKNTKGGVL CECSDPLVLG EDGRTCVDAG RLPRASVVSI 850
ALGAVLVCGL AGLAWTVVCR WTHADARPLL PVGEGEGDGK SPSLPLPGCG 900
NRRDVGAAPA LEVEQDLSCG SRGLCE 926
Length:926
Mass (Da):100,443
Last modified:July 1, 1989 - v1
Checksum:i8549FF6F0F742C5E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X04645 mRNA. Translation: CAA28306.1.
PIRiA27416. OPPGIT.
UniGeneiSsc.99.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X04645 mRNA. Translation: CAA28306.1 .
PIRi A27416. OPPGIT.
UniGenei Ssc.99.

3D structure databases

ProteinModelPortali P09933.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9823.ENSSSCP00000009228.

Protein family/group databases

PeroxiBasei 3329. SscTPO.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi NOG262194.
HOGENOMi HOG000016084.
HOVERGENi HBG000071.

Enzyme and pathway databases

UniPathwayi UPA00194 .
BioCyci MetaCyc:MONOMER-14809.

Family and domain databases

Gene3Di 1.10.640.10. 1 hit.
InterProi IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR010255. Haem_peroxidase.
IPR019791. Haem_peroxidase_animal.
IPR000436. Sushi_SCR_CCP.
IPR029589. TPO.
[Graphical view ]
PANTHERi PTHR11475:SF60. PTHR11475:SF60. 1 hit.
Pfami PF03098. An_peroxidase. 1 hit.
PF07645. EGF_CA. 1 hit.
PF00084. Sushi. 1 hit.
[Graphical view ]
PRINTSi PR00457. ANPEROXIDASE.
SMARTi SM00032. CCP. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view ]
SUPFAMi SSF48113. SSF48113. 1 hit.
SSF57535. SSF57535. 1 hit.
PROSITEi PS50026. EGF_3. 1 hit.
PS00435. PEROXIDASE_1. 1 hit.
PS50292. PEROXIDASE_3. 1 hit.
PS50923. SUSHI. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of the structural gene for porcine thyroid peroxidase."
    Magnusson R.P., Gestautas J., Taurog A., Rapoport B.
    J. Biol. Chem. 262:13885-13888(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation and characterization of a cDNA clone for porcine thyroid peroxidase."
    Magnusson R.P., Gestautas J., Seto P., Taurog A., Rapoport B.
    FEBS Lett. 208:391-396(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 595-926.
  3. "Thyroid peroxidase glycosylation: the location and nature of the N-linked oligosaccharide units in porcine thyroid peroxidase."
    Rawitch A.B., Pollock G., Yang S.-X., Taurog A.
    Arch. Biochem. Biophys. 297:321-327(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 110-115; 267-274; 301-308 AND 340-351, GLYCOSYLATION AT ASN-129; ASN-277; ASN-307 AND ASN-342.

Entry informationi

Entry nameiPERT_PIG
AccessioniPrimary (citable) accession number: P09933
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: September 3, 2014
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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