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P09923 (PPBI_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 147. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Intestinal-type alkaline phosphatase

Short name=IAP
Short name=Intestinal alkaline phosphatase
EC=3.1.3.1
Gene names
Name:ALPI
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length528 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion By similarity.

Binds 2 zinc ions By similarity.

Subunit structure

Homodimer.

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor.

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Sequence similarities

Belongs to the alkaline phosphatase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Ref.10 Ref.11
Chain20 – 503484Intestinal-type alkaline phosphatase
PRO_0000024037
Propeptide504 – 52825Removed in mature form By similarity
PRO_0000024038

Sites

Active site1111Phosphoserine intermediate
Metal binding611Magnesium By similarity
Metal binding611Zinc 1 By similarity
Metal binding1111Zinc 1 By similarity
Metal binding1741Magnesium By similarity
Metal binding3301Magnesium By similarity
Metal binding3351Zinc 2 By similarity
Metal binding3391Zinc 2; via tele nitrogen By similarity
Metal binding3761Zinc 1 By similarity
Metal binding3771Zinc 1; via tele nitrogen By similarity
Metal binding4511Zinc 2; via tele nitrogen By similarity

Amino acid modifications

Lipidation5031GPI-anchor amidated aspartate By similarity
Glycosylation1411N-linked (GlcNAc...) Potential
Glycosylation2681N-linked (GlcNAc...) Potential
Glycosylation4291N-linked (GlcNAc...) Potential
Disulfide bond140 ↔ 202 By similarity
Disulfide bond486 ↔ 493 By similarity

Natural variations

Natural variant1441R → H.
Corresponds to variant rs7559279 [ dbSNP | Ensembl ].
VAR_050524
Natural variant2981H → L.
Corresponds to variant rs1047223 [ dbSNP | Ensembl ].
VAR_011816

Experimental info

Sequence conflict3471L → V in AAA51703. Ref.2
Sequence conflict4101I → T in AAA51704. Ref.1
Sequence conflict4971P → L in AAA51703. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P09923 [UniParc].

Last modified April 1, 1990. Version 2.
Checksum: 465306BEDF9F0B79

FASTA52856,812
        10         20         30         40         50         60 
MQGPWVLLLL GLRLQLSLGV IPAEEENPAF WNRQAAEALD AAKKLQPIQK VAKNLILFLG 

        70         80         90        100        110        120 
DGLGVPTVTA TRILKGQKNG KLGPETPLAM DRFPYLALSK TYNVDRQVPD SAATATAYLC 

       130        140        150        160        170        180 
GVKANFQTIG LSAAARFNQC NTTRGNEVIS VMNRAKQAGK SVGVVTTTRV QHASPAGTYA 

       190        200        210        220        230        240 
HTVNRNWYSD ADMPASARQE GCQDIATQLI SNMDIDVILG GGRKYMFPMG TPDPEYPADA 

       250        260        270        280        290        300 
SQNGIRLDGK NLVQEWLAKH QGAWYVWNRT ELMQASLDQS VTHLMGLFEP GDTKYEIHRD 

       310        320        330        340        350        360 
PTLDPSLMEM TEAALRLLSR NPRGFYLFVE GGRIDHGHHE GVAYQALTEA VMFDDAIERA 

       370        380        390        400        410        420 
GQLTSEEDTL TLVTADHSHV FSFGGYTLRG SSIFGLAPSK AQDSKAYTSI LYGNGPGYVF 

       430        440        450        460        470        480 
NSGVRPDVNE SESGSPDYQQ QAAVPLSSET HGGEDVAVFA RGPQAHLVHG VQEQSFVAHV 

       490        500        510        520 
MAFAACLEPY TACDLAPPAC TTDAAHPVAA SLPLLAGTLL LLGASAAP 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequencing of human intestinal alkaline phosphatase cDNA."
Berger J., Garattini E., Hua J.-C., Udenfriend S.
Proc. Natl. Acad. Sci. U.S.A. 84:695-698(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]"Nucleotide and amino acid sequences of human intestinal alkaline phosphatase: close homology to placental alkaline phosphatase."
Henthorn P.S., Raducha M., Edwards Y.H., Weiss M.J., Slaughter C., Lafferty M.A., Harris H.
Proc. Natl. Acad. Sci. U.S.A. 84:1234-1238(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Sequence and characterization of the human intestinal alkaline phosphatase gene."
Henthorn P.S., Raducha M., Kadesch T., Weiss M.J., Harris H.
J. Biol. Chem. 263:12011-12019(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]"Promoter structure of the human intestinal alkaline phosphatase gene."
Millan J.L.
Nucleic Acids Res. 15:10599-10599(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-100.
[9]"Two gene duplication events in the evolution of the human heat-stable alkaline phosphatases."
Knoll B.J., Rothblum K.N., Longley M.
Gene 60:267-276(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-73.
[10]"Partial sequencing of human adult, human fetal, and bovine intestinal alkaline phosphatases: comparison with the human placental and liver isozymes."
Hua J.-C., Berger J., Pan Y.C.E., Hulmes J.D., Udenfriend S.
Proc. Natl. Acad. Sci. U.S.A. 83:2368-2372(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 20-58.
[11]"Chemical nature of intestinal-type alkaline phosphatase in human kidney."
Nishihara Y., Hayashi Y., Adachi T., Koyama I., Stigbrand T., Hirano K.
Clin. Chem. 38:2539-2542(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 20-49.
[12]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Web resources

Wikipedia

Alkaline phosphatase entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M15694 mRNA. Translation: AAA51703.1.
M31008 mRNA. Translation: AAA51704.1.
J03930 Genomic DNA. Translation: AAA98617.1.
AK313163 mRNA. Translation: BAG35981.1.
AC068134 Genomic DNA. Translation: AAY24089.1.
CH471063 Genomic DNA. Translation: EAW70997.1.
BC132678 mRNA. Translation: AAI32679.1.
Y00512 Genomic DNA. Translation: CAA68564.1.
M19161 Genomic DNA. Translation: AAA51705.1.
CCDSCCDS2492.1.
PIRPAHUI. A31073.
RefSeqNP_001622.2. NM_001631.3.
UniGeneHs.284255.
Hs.37009.

3D structure databases

ProteinModelPortalP09923.
SMRP09923. Positions 20-499.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid106749. 4 interactions.
IntActP09923. 1 interaction.
MINTMINT-2800762.
STRING9606.ENSP00000295463.

Chemistry

BindingDBP09923.
ChEMBLCHEMBL5573.

PTM databases

PhosphoSiteP09923.
UniCarbKBP09923.

Polymorphism databases

DMDM130744.

Proteomic databases

MaxQBP09923.
PaxDbP09923.
PRIDEP09923.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000295463; ENSP00000295463; ENSG00000163295.
GeneID248.
KEGGhsa:248.
UCSCuc002vst.4. human.

Organism-specific databases

CTD248.
GeneCardsGC02P233320.
HGNCHGNC:437. ALPI.
MIM171740. gene.
neXtProtNX_P09923.
PharmGKBPA24728.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1785.
HOGENOMHOG000099118.
HOVERGENHBG007345.
InParanoidP09923.
KOK01077.
OMAKYMFPTG.
OrthoDBEOG7SN8CH.
PhylomeDBP09923.
TreeFamTF323513.

Enzyme and pathway databases

SABIO-RKP09923.

Gene expression databases

ArrayExpressP09923.
BgeeP09923.
CleanExHS_ALPI.
GenevestigatorP09923.

Family and domain databases

Gene3D3.40.720.10. 1 hit.
InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSPR00113. ALKPHPHTASE.
SMARTSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMSSF53649. SSF53649. 1 hit.
PROSITEPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSALPI. human.
GeneWikiALPI.
GenomeRNAi248.
NextBio993.
PROP09923.
SOURCESearch...

Entry information

Entry namePPBI_HUMAN
AccessionPrimary (citable) accession number: P09923
Secondary accession number(s): B2R7Y4 expand/collapse secondary AC list , Q53S80, Q9UBV5, Q9UCL2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: April 1, 1990
Last modified: July 9, 2014
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM