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Protein

Intestinal-type alkaline phosphatase

Gene

ALPI

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611MagnesiumBy similarity
Metal bindingi61 – 611Zinc 1By similarity
Active sitei111 – 1111Phosphoserine intermediate
Metal bindingi111 – 1111Zinc 1By similarity
Metal bindingi174 – 1741MagnesiumBy similarity
Metal bindingi330 – 3301MagnesiumBy similarity
Metal bindingi335 – 3351Zinc 2By similarity
Metal bindingi339 – 3391Zinc 2; via tele nitrogenBy similarity
Metal bindingi376 – 3761Zinc 1By similarity
Metal bindingi377 – 3771Zinc 1; via tele nitrogenBy similarity
Metal bindingi451 – 4511Zinc 2; via tele nitrogenBy similarity

GO - Molecular functioni

  1. alkaline phosphatase activity Source: UniProtKB
  2. magnesium ion binding Source: UniProtKB
  3. protease binding Source: ParkinsonsUK-UCL
  4. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. dephosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

SABIO-RKP09923.

Names & Taxonomyi

Protein namesi
Recommended name:
Intestinal-type alkaline phosphatase (EC:3.1.3.1)
Short name:
IAP
Short name:
Intestinal alkaline phosphatase
Gene namesi
Name:ALPI
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:437. ALPI.

Subcellular locationi

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24728.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 19192 PublicationsAdd
BLAST
Chaini20 – 503484Intestinal-type alkaline phosphatasePRO_0000024037Add
BLAST
Propeptidei504 – 52825Removed in mature formBy similarityPRO_0000024038Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi140 ↔ 202By similarity
Glycosylationi141 – 1411N-linked (GlcNAc...)Sequence Analysis
Glycosylationi268 – 2681N-linked (GlcNAc...)Sequence Analysis
Glycosylationi429 – 4291N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi486 ↔ 493By similarity
Lipidationi503 – 5031GPI-anchor amidated aspartateBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

MaxQBiP09923.
PaxDbiP09923.
PRIDEiP09923.

PTM databases

DEPODiP09923.
PhosphoSiteiP09923.
UniCarbKBiP09923.

Expressioni

Gene expression databases

BgeeiP09923.
CleanExiHS_ALPI.
ExpressionAtlasiP09923. baseline and differential.
GenevestigatoriP09923.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi106749. 8 interactions.
IntActiP09923. 2 interactions.
MINTiMINT-2800762.
STRINGi9606.ENSP00000295463.

Structurei

3D structure databases

ProteinModelPortaliP09923.
SMRiP09923. Positions 20-499.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal, Transmembrane

Phylogenomic databases

eggNOGiCOG1785.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP09923.
KOiK01077.
OMAiHIRTAIT.
OrthoDBiEOG7SN8CH.
PhylomeDBiP09923.
TreeFamiTF323513.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09923-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQGPWVLLLL GLRLQLSLGV IPAEEENPAF WNRQAAEALD AAKKLQPIQK
60 70 80 90 100
VAKNLILFLG DGLGVPTVTA TRILKGQKNG KLGPETPLAM DRFPYLALSK
110 120 130 140 150
TYNVDRQVPD SAATATAYLC GVKANFQTIG LSAAARFNQC NTTRGNEVIS
160 170 180 190 200
VMNRAKQAGK SVGVVTTTRV QHASPAGTYA HTVNRNWYSD ADMPASARQE
210 220 230 240 250
GCQDIATQLI SNMDIDVILG GGRKYMFPMG TPDPEYPADA SQNGIRLDGK
260 270 280 290 300
NLVQEWLAKH QGAWYVWNRT ELMQASLDQS VTHLMGLFEP GDTKYEIHRD
310 320 330 340 350
PTLDPSLMEM TEAALRLLSR NPRGFYLFVE GGRIDHGHHE GVAYQALTEA
360 370 380 390 400
VMFDDAIERA GQLTSEEDTL TLVTADHSHV FSFGGYTLRG SSIFGLAPSK
410 420 430 440 450
AQDSKAYTSI LYGNGPGYVF NSGVRPDVNE SESGSPDYQQ QAAVPLSSET
460 470 480 490 500
HGGEDVAVFA RGPQAHLVHG VQEQSFVAHV MAFAACLEPY TACDLAPPAC
510 520
TTDAAHPVAA SLPLLAGTLL LLGASAAP
Length:528
Mass (Da):56,812
Last modified:April 1, 1990 - v2
Checksum:i465306BEDF9F0B79
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti347 – 3471L → V in AAA51703. (PubMed:3469665)Curated
Sequence conflicti410 – 4101I → T in AAA51704. (PubMed:3468508)Curated
Sequence conflicti497 – 4971P → L in AAA51703. (PubMed:3469665)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti144 – 1441R → H.
Corresponds to variant rs7559279 [ dbSNP | Ensembl ].
VAR_050524
Natural varianti298 – 2981H → L.
Corresponds to variant rs1047223 [ dbSNP | Ensembl ].
VAR_011816

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15694 mRNA. Translation: AAA51703.1.
M31008 mRNA. Translation: AAA51704.1.
J03930 Genomic DNA. Translation: AAA98617.1.
AK313163 mRNA. Translation: BAG35981.1.
AC068134 Genomic DNA. Translation: AAY24089.1.
CH471063 Genomic DNA. Translation: EAW70997.1.
BC132678 mRNA. Translation: AAI32679.1.
Y00512 Genomic DNA. Translation: CAA68564.1.
M19161 Genomic DNA. Translation: AAA51705.1.
CCDSiCCDS2492.1.
PIRiA31073. PAHUI.
RefSeqiNP_001622.2. NM_001631.3.
UniGeneiHs.284255.
Hs.37009.

Genome annotation databases

EnsembliENST00000295463; ENSP00000295463; ENSG00000163295.
GeneIDi248.
KEGGihsa:248.
UCSCiuc002vst.4. human.

Polymorphism databases

DMDMi130744.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Wikipedia

Alkaline phosphatase entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15694 mRNA. Translation: AAA51703.1.
M31008 mRNA. Translation: AAA51704.1.
J03930 Genomic DNA. Translation: AAA98617.1.
AK313163 mRNA. Translation: BAG35981.1.
AC068134 Genomic DNA. Translation: AAY24089.1.
CH471063 Genomic DNA. Translation: EAW70997.1.
BC132678 mRNA. Translation: AAI32679.1.
Y00512 Genomic DNA. Translation: CAA68564.1.
M19161 Genomic DNA. Translation: AAA51705.1.
CCDSiCCDS2492.1.
PIRiA31073. PAHUI.
RefSeqiNP_001622.2. NM_001631.3.
UniGeneiHs.284255.
Hs.37009.

3D structure databases

ProteinModelPortaliP09923.
SMRiP09923. Positions 20-499.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106749. 8 interactions.
IntActiP09923. 2 interactions.
MINTiMINT-2800762.
STRINGi9606.ENSP00000295463.

Chemistry

BindingDBiP09923.
ChEMBLiCHEMBL5573.

PTM databases

DEPODiP09923.
PhosphoSiteiP09923.
UniCarbKBiP09923.

Polymorphism databases

DMDMi130744.

Proteomic databases

MaxQBiP09923.
PaxDbiP09923.
PRIDEiP09923.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000295463; ENSP00000295463; ENSG00000163295.
GeneIDi248.
KEGGihsa:248.
UCSCiuc002vst.4. human.

Organism-specific databases

CTDi248.
GeneCardsiGC02P233320.
HGNCiHGNC:437. ALPI.
MIMi171740. gene.
neXtProtiNX_P09923.
PharmGKBiPA24728.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1785.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP09923.
KOiK01077.
OMAiHIRTAIT.
OrthoDBiEOG7SN8CH.
PhylomeDBiP09923.
TreeFamiTF323513.

Enzyme and pathway databases

SABIO-RKP09923.

Miscellaneous databases

ChiTaRSiALPI. human.
GeneWikiiALPI.
GenomeRNAii248.
NextBioi993.
PROiP09923.
SOURCEiSearch...

Gene expression databases

BgeeiP09923.
CleanExiHS_ALPI.
ExpressionAtlasiP09923. baseline and differential.
GenevestigatoriP09923.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of human intestinal alkaline phosphatase cDNA."
    Berger J., Garattini E., Hua J.-C., Udenfriend S.
    Proc. Natl. Acad. Sci. U.S.A. 84:695-698(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  2. "Nucleotide and amino acid sequences of human intestinal alkaline phosphatase: close homology to placental alkaline phosphatase."
    Henthorn P.S., Raducha M., Edwards Y.H., Weiss M.J., Slaughter C., Lafferty M.A., Harris H.
    Proc. Natl. Acad. Sci. U.S.A. 84:1234-1238(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Sequence and characterization of the human intestinal alkaline phosphatase gene."
    Henthorn P.S., Raducha M., Kadesch T., Weiss M.J., Harris H.
    J. Biol. Chem. 263:12011-12019(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  8. "Promoter structure of the human intestinal alkaline phosphatase gene."
    Millan J.L.
    Nucleic Acids Res. 15:10599-10599(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-100.
  9. "Two gene duplication events in the evolution of the human heat-stable alkaline phosphatases."
    Knoll B.J., Rothblum K.N., Longley M.
    Gene 60:267-276(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-73.
  10. "Partial sequencing of human adult, human fetal, and bovine intestinal alkaline phosphatases: comparison with the human placental and liver isozymes."
    Hua J.-C., Berger J., Pan Y.C.E., Hulmes J.D., Udenfriend S.
    Proc. Natl. Acad. Sci. U.S.A. 83:2368-2372(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 20-58.
  11. "Chemical nature of intestinal-type alkaline phosphatase in human kidney."
    Nishihara Y., Hayashi Y., Adachi T., Koyama I., Stigbrand T., Hirano K.
    Clin. Chem. 38:2539-2542(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 20-49.
  12. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiPPBI_HUMAN
AccessioniPrimary (citable) accession number: P09923
Secondary accession number(s): B2R7Y4
, Q53S80, Q9UBV5, Q9UCL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: April 1, 1990
Last modified: February 4, 2015
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.