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P09923

- PPBI_HUMAN

UniProt

P09923 - PPBI_HUMAN

Protein

Intestinal-type alkaline phosphatase

Gene

ALPI

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 149 (01 Oct 2014)
      Sequence version 2 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

    Cofactori

    Binds 1 magnesium ion.By similarity
    Binds 2 zinc ions.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi61 – 611MagnesiumBy similarity
    Metal bindingi61 – 611Zinc 1By similarity
    Active sitei111 – 1111Phosphoserine intermediate
    Metal bindingi111 – 1111Zinc 1By similarity
    Metal bindingi174 – 1741MagnesiumBy similarity
    Metal bindingi330 – 3301MagnesiumBy similarity
    Metal bindingi335 – 3351Zinc 2By similarity
    Metal bindingi339 – 3391Zinc 2; via tele nitrogenBy similarity
    Metal bindingi376 – 3761Zinc 1By similarity
    Metal bindingi377 – 3771Zinc 1; via tele nitrogenBy similarity
    Metal bindingi451 – 4511Zinc 2; via tele nitrogenBy similarity

    GO - Molecular functioni

    1. alkaline phosphatase activity Source: UniProtKB
    2. magnesium ion binding Source: UniProtKB
    3. protease binding Source: ParkinsonsUK-UCL
    4. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. dephosphorylation Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    SABIO-RKP09923.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Intestinal-type alkaline phosphatase (EC:3.1.3.1)
    Short name:
    IAP
    Short name:
    Intestinal alkaline phosphatase
    Gene namesi
    Name:ALPI
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:437. ALPI.

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA24728.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 19192 PublicationsAdd
    BLAST
    Chaini20 – 503484Intestinal-type alkaline phosphatasePRO_0000024037Add
    BLAST
    Propeptidei504 – 52825Removed in mature formBy similarityPRO_0000024038Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi140 ↔ 202By similarity
    Glycosylationi141 – 1411N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi268 – 2681N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi429 – 4291N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi486 ↔ 493By similarity
    Lipidationi503 – 5031GPI-anchor amidated aspartateBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP09923.
    PaxDbiP09923.
    PRIDEiP09923.

    PTM databases

    PhosphoSiteiP09923.
    UniCarbKBiP09923.

    Expressioni

    Gene expression databases

    ArrayExpressiP09923.
    BgeeiP09923.
    CleanExiHS_ALPI.
    GenevestigatoriP09923.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    BioGridi106749. 4 interactions.
    IntActiP09923. 1 interaction.
    MINTiMINT-2800762.
    STRINGi9606.ENSP00000295463.

    Structurei

    3D structure databases

    ProteinModelPortaliP09923.
    SMRiP09923. Positions 20-499.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alkaline phosphatase family.Curated

    Keywords - Domaini

    Signal, Transmembrane

    Phylogenomic databases

    eggNOGiCOG1785.
    HOGENOMiHOG000099118.
    HOVERGENiHBG007345.
    InParanoidiP09923.
    KOiK01077.
    OMAiKYMFPTG.
    OrthoDBiEOG7SN8CH.
    PhylomeDBiP09923.
    TreeFamiTF323513.

    Family and domain databases

    Gene3Di3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view]
    PfamiPF00245. Alk_phosphatase. 1 hit.
    [Graphical view]
    PRINTSiPR00113. ALKPHPHTASE.
    SMARTiSM00098. alkPPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 1 hit.
    PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09923-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQGPWVLLLL GLRLQLSLGV IPAEEENPAF WNRQAAEALD AAKKLQPIQK    50
    VAKNLILFLG DGLGVPTVTA TRILKGQKNG KLGPETPLAM DRFPYLALSK 100
    TYNVDRQVPD SAATATAYLC GVKANFQTIG LSAAARFNQC NTTRGNEVIS 150
    VMNRAKQAGK SVGVVTTTRV QHASPAGTYA HTVNRNWYSD ADMPASARQE 200
    GCQDIATQLI SNMDIDVILG GGRKYMFPMG TPDPEYPADA SQNGIRLDGK 250
    NLVQEWLAKH QGAWYVWNRT ELMQASLDQS VTHLMGLFEP GDTKYEIHRD 300
    PTLDPSLMEM TEAALRLLSR NPRGFYLFVE GGRIDHGHHE GVAYQALTEA 350
    VMFDDAIERA GQLTSEEDTL TLVTADHSHV FSFGGYTLRG SSIFGLAPSK 400
    AQDSKAYTSI LYGNGPGYVF NSGVRPDVNE SESGSPDYQQ QAAVPLSSET 450
    HGGEDVAVFA RGPQAHLVHG VQEQSFVAHV MAFAACLEPY TACDLAPPAC 500
    TTDAAHPVAA SLPLLAGTLL LLGASAAP 528
    Length:528
    Mass (Da):56,812
    Last modified:April 1, 1990 - v2
    Checksum:i465306BEDF9F0B79
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti347 – 3471L → V in AAA51703. (PubMed:3469665)Curated
    Sequence conflicti410 – 4101I → T in AAA51704. (PubMed:3468508)Curated
    Sequence conflicti497 – 4971P → L in AAA51703. (PubMed:3469665)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti144 – 1441R → H.
    Corresponds to variant rs7559279 [ dbSNP | Ensembl ].
    VAR_050524
    Natural varianti298 – 2981H → L.
    Corresponds to variant rs1047223 [ dbSNP | Ensembl ].
    VAR_011816

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15694 mRNA. Translation: AAA51703.1.
    M31008 mRNA. Translation: AAA51704.1.
    J03930 Genomic DNA. Translation: AAA98617.1.
    AK313163 mRNA. Translation: BAG35981.1.
    AC068134 Genomic DNA. Translation: AAY24089.1.
    CH471063 Genomic DNA. Translation: EAW70997.1.
    BC132678 mRNA. Translation: AAI32679.1.
    Y00512 Genomic DNA. Translation: CAA68564.1.
    M19161 Genomic DNA. Translation: AAA51705.1.
    CCDSiCCDS2492.1.
    PIRiA31073. PAHUI.
    RefSeqiNP_001622.2. NM_001631.3.
    UniGeneiHs.284255.
    Hs.37009.

    Genome annotation databases

    EnsembliENST00000295463; ENSP00000295463; ENSG00000163295.
    GeneIDi248.
    KEGGihsa:248.
    UCSCiuc002vst.4. human.

    Polymorphism databases

    DMDMi130744.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    Wikipedia

    Alkaline phosphatase entry

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15694 mRNA. Translation: AAA51703.1 .
    M31008 mRNA. Translation: AAA51704.1 .
    J03930 Genomic DNA. Translation: AAA98617.1 .
    AK313163 mRNA. Translation: BAG35981.1 .
    AC068134 Genomic DNA. Translation: AAY24089.1 .
    CH471063 Genomic DNA. Translation: EAW70997.1 .
    BC132678 mRNA. Translation: AAI32679.1 .
    Y00512 Genomic DNA. Translation: CAA68564.1 .
    M19161 Genomic DNA. Translation: AAA51705.1 .
    CCDSi CCDS2492.1.
    PIRi A31073. PAHUI.
    RefSeqi NP_001622.2. NM_001631.3.
    UniGenei Hs.284255.
    Hs.37009.

    3D structure databases

    ProteinModelPortali P09923.
    SMRi P09923. Positions 20-499.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 106749. 4 interactions.
    IntActi P09923. 1 interaction.
    MINTi MINT-2800762.
    STRINGi 9606.ENSP00000295463.

    Chemistry

    BindingDBi P09923.
    ChEMBLi CHEMBL5573.

    PTM databases

    PhosphoSitei P09923.
    UniCarbKBi P09923.

    Polymorphism databases

    DMDMi 130744.

    Proteomic databases

    MaxQBi P09923.
    PaxDbi P09923.
    PRIDEi P09923.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000295463 ; ENSP00000295463 ; ENSG00000163295 .
    GeneIDi 248.
    KEGGi hsa:248.
    UCSCi uc002vst.4. human.

    Organism-specific databases

    CTDi 248.
    GeneCardsi GC02P233320.
    HGNCi HGNC:437. ALPI.
    MIMi 171740. gene.
    neXtProti NX_P09923.
    PharmGKBi PA24728.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1785.
    HOGENOMi HOG000099118.
    HOVERGENi HBG007345.
    InParanoidi P09923.
    KOi K01077.
    OMAi KYMFPTG.
    OrthoDBi EOG7SN8CH.
    PhylomeDBi P09923.
    TreeFami TF323513.

    Enzyme and pathway databases

    SABIO-RK P09923.

    Miscellaneous databases

    ChiTaRSi ALPI. human.
    GeneWikii ALPI.
    GenomeRNAii 248.
    NextBioi 993.
    PROi P09923.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P09923.
    Bgeei P09923.
    CleanExi HS_ALPI.
    Genevestigatori P09923.

    Family and domain databases

    Gene3Di 3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view ]
    Pfami PF00245. Alk_phosphatase. 1 hit.
    [Graphical view ]
    PRINTSi PR00113. ALKPHPHTASE.
    SMARTi SM00098. alkPPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 1 hit.
    PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of human intestinal alkaline phosphatase cDNA."
      Berger J., Garattini E., Hua J.-C., Udenfriend S.
      Proc. Natl. Acad. Sci. U.S.A. 84:695-698(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    2. "Nucleotide and amino acid sequences of human intestinal alkaline phosphatase: close homology to placental alkaline phosphatase."
      Henthorn P.S., Raducha M., Edwards Y.H., Weiss M.J., Slaughter C., Lafferty M.A., Harris H.
      Proc. Natl. Acad. Sci. U.S.A. 84:1234-1238(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Sequence and characterization of the human intestinal alkaline phosphatase gene."
      Henthorn P.S., Raducha M., Kadesch T., Weiss M.J., Harris H.
      J. Biol. Chem. 263:12011-12019(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    8. "Promoter structure of the human intestinal alkaline phosphatase gene."
      Millan J.L.
      Nucleic Acids Res. 15:10599-10599(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-100.
    9. "Two gene duplication events in the evolution of the human heat-stable alkaline phosphatases."
      Knoll B.J., Rothblum K.N., Longley M.
      Gene 60:267-276(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-73.
    10. "Partial sequencing of human adult, human fetal, and bovine intestinal alkaline phosphatases: comparison with the human placental and liver isozymes."
      Hua J.-C., Berger J., Pan Y.C.E., Hulmes J.D., Udenfriend S.
      Proc. Natl. Acad. Sci. U.S.A. 83:2368-2372(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 20-58.
    11. "Chemical nature of intestinal-type alkaline phosphatase in human kidney."
      Nishihara Y., Hayashi Y., Adachi T., Koyama I., Stigbrand T., Hirano K.
      Clin. Chem. 38:2539-2542(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 20-49.
    12. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.

    Entry informationi

    Entry nameiPPBI_HUMAN
    AccessioniPrimary (citable) accession number: P09923
    Secondary accession number(s): B2R7Y4
    , Q53S80, Q9UBV5, Q9UCL2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 149 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3