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Protein

Intestinal-type alkaline phosphatase

Gene

ALPI

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi61MagnesiumBy similarity1
Metal bindingi61Zinc 1By similarity1
Active sitei111Phosphoserine intermediate1
Metal bindingi111Zinc 1By similarity1
Metal bindingi174MagnesiumBy similarity1
Metal bindingi330MagnesiumBy similarity1
Metal bindingi335Zinc 2By similarity1
Metal bindingi339Zinc 2; via tele nitrogenBy similarity1
Metal bindingi376Zinc 1By similarity1
Metal bindingi377Zinc 1; via tele nitrogenBy similarity1
Metal bindingi451Zinc 2; via tele nitrogenBy similarity1

GO - Molecular functioni

  • alkaline phosphatase activity Source: UniProtKB
  • magnesium ion binding Source: UniProtKB
  • protease binding Source: ParkinsonsUK-UCL
  • zinc ion binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-1483166. Synthesis of PA.
R-HSA-163125. Post-translational modification: synthesis of GPI-anchored proteins.
R-HSA-8935690. Digestion.
SABIO-RKiP09923.

Names & Taxonomyi

Protein namesi
Recommended name:
Intestinal-type alkaline phosphatase (EC:3.1.3.1)
Short name:
IAP
Short name:
Intestinal alkaline phosphatase
Gene namesi
Name:ALPI
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000163295.4.
HGNCiHGNC:437. ALPI.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi248.
OpenTargetsiENSG00000163295.
PharmGKBiPA24728.

Chemistry databases

ChEMBLiCHEMBL5573.

Polymorphism and mutation databases

BioMutaiALPI.
DMDMi130744.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 192 PublicationsAdd BLAST19
ChainiPRO_000002403720 – 503Intestinal-type alkaline phosphataseAdd BLAST484
PropeptideiPRO_0000024038504 – 528Removed in mature formBy similarityAdd BLAST25

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi140 ↔ 202By similarity
Glycosylationi141N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi268N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi429N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi486 ↔ 493By similarity
Lipidationi503GPI-anchor amidated aspartateBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

EPDiP09923.
MaxQBiP09923.
PaxDbiP09923.
PeptideAtlasiP09923.
PRIDEiP09923.

PTM databases

DEPODiP09923.
iPTMnetiP09923.
PhosphoSitePlusiP09923.
UniCarbKBiP09923.

Expressioni

Gene expression databases

BgeeiENSG00000163295.
CleanExiHS_ALPI.
ExpressionAtlasiP09923. baseline and differential.
GenevisibleiP09923. HS.

Organism-specific databases

HPAiHPA038764.
HPA038765.
HPA051699.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

Show more details

GO - Molecular functioni

  • protease binding Source: ParkinsonsUK-UCL

Protein-protein interaction databases

BioGridi106749. 18 interactors.
IntActiP09923. 9 interactors.
MINTiMINT-2800762.
STRINGi9606.ENSP00000295463.

Chemistry databases

BindingDBiP09923.

Structurei

3D structure databases

ProteinModelPortaliP09923.
SMRiP09923.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal, Transmembrane

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP09923.
KOiK01077.
OMAiYVWSRKG.
OrthoDBiEOG091G067H.
PhylomeDBiP09923.
TreeFamiTF323513.

Family and domain databases

CDDicd16012. ALP. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiView protein in InterPro
IPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
PANTHERiPTHR11596. PTHR11596. 1 hit.
PfamiView protein in Pfam
PF00245. Alk_phosphatase. 1 hit.
PRINTSiPR00113. ALKPHPHTASE.
SMARTiView protein in SMART
SM00098. alkPPc. 1 hit.
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiView protein in PROSITE
PS00123. ALKALINE_PHOSPHATASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09923-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQGPWVLLLL GLRLQLSLGV IPAEEENPAF WNRQAAEALD AAKKLQPIQK
60 70 80 90 100
VAKNLILFLG DGLGVPTVTA TRILKGQKNG KLGPETPLAM DRFPYLALSK
110 120 130 140 150
TYNVDRQVPD SAATATAYLC GVKANFQTIG LSAAARFNQC NTTRGNEVIS
160 170 180 190 200
VMNRAKQAGK SVGVVTTTRV QHASPAGTYA HTVNRNWYSD ADMPASARQE
210 220 230 240 250
GCQDIATQLI SNMDIDVILG GGRKYMFPMG TPDPEYPADA SQNGIRLDGK
260 270 280 290 300
NLVQEWLAKH QGAWYVWNRT ELMQASLDQS VTHLMGLFEP GDTKYEIHRD
310 320 330 340 350
PTLDPSLMEM TEAALRLLSR NPRGFYLFVE GGRIDHGHHE GVAYQALTEA
360 370 380 390 400
VMFDDAIERA GQLTSEEDTL TLVTADHSHV FSFGGYTLRG SSIFGLAPSK
410 420 430 440 450
AQDSKAYTSI LYGNGPGYVF NSGVRPDVNE SESGSPDYQQ QAAVPLSSET
460 470 480 490 500
HGGEDVAVFA RGPQAHLVHG VQEQSFVAHV MAFAACLEPY TACDLAPPAC
510 520
TTDAAHPVAA SLPLLAGTLL LLGASAAP
Length:528
Mass (Da):56,812
Last modified:April 1, 1990 - v2
Checksum:i465306BEDF9F0B79
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti347L → V in AAA51703 (PubMed:3469665).Curated1
Sequence conflicti410I → T in AAA51704 (PubMed:3468508).Curated1
Sequence conflicti497P → L in AAA51703 (PubMed:3469665).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_050524144R → H. Corresponds to variant dbSNP:rs7559279Ensembl.1
Natural variantiVAR_011816298H → L. Corresponds to variant dbSNP:rs1047223Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15694 mRNA. Translation: AAA51703.1.
M31008 mRNA. Translation: AAA51704.1.
J03930 Genomic DNA. Translation: AAA98617.1.
AK313163 mRNA. Translation: BAG35981.1.
AC068134 Genomic DNA. Translation: AAY24089.1.
CH471063 Genomic DNA. Translation: EAW70997.1.
BC132678 mRNA. Translation: AAI32679.1.
Y00512 Genomic DNA. Translation: CAA68564.1.
M19161 Genomic DNA. Translation: AAA51705.1.
CCDSiCCDS2492.1.
PIRiA31073. PAHUI.
RefSeqiNP_001622.2. NM_001631.4.
UniGeneiHs.284255.
Hs.37009.

Genome annotation databases

EnsembliENST00000295463; ENSP00000295463; ENSG00000163295.
GeneIDi248.
KEGGihsa:248.
UCSCiuc002vst.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPPBI_HUMAN
AccessioniPrimary (citable) accession number: P09923
Secondary accession number(s): B2R7Y4
, Q53S80, Q9UBV5, Q9UCL2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: April 1, 1990
Last modified: September 27, 2017
This is version 178 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families