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P09922

- MX1_MOUSE

UniProt

P09922 - MX1_MOUSE

Protein

Interferon-induced GTP-binding protein Mx1

Gene

Mx1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Interferon-induced dynamin-like GTPase with antiviral activity against influenza A virus, (IAV), influenza B virus (IBV) and Thogoto virus (THOV). Inhibits FLUAV by interfering with the process of primary transcription, probably by affecting the viral polymerase function.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi43 – 508GTPSequence Analysis
    Nucleotide bindingi144 – 1485GTPSequence Analysis
    Nucleotide bindingi213 – 2164GTPSequence Analysis

    GO - Molecular functioni

    1. GTPase activity Source: MGI
    2. GTP binding Source: MGI
    3. protein binding Source: UniProtKB

    GO - Biological processi

    1. defense response to virus Source: UniProtKB-KW
    2. GTP catabolic process Source: GOC
    3. innate immune response Source: UniProtKB
    4. response to virus Source: UniProtKB

    Keywords - Biological processi

    Antiviral defense, Immunity, Innate immunity

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Interferon-induced GTP-binding protein Mx1
    Alternative name(s):
    Influenza resistance protein
    Myxoma resistance protein 1
    Myxovirus resistance protein 1
    Gene namesi
    Name:Mx1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:97243. Mx1.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi47 – 471S → C: No effect on viral infection. 1 Publication
    Mutagenesisi49 – 491K → A or M: Loss of protection against viral infection. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 631631Interferon-induced GTP-binding protein Mx1PRO_0000206593Add
    BLAST

    Post-translational modificationi

    ISGylated.By similarity

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    PRIDEiP09922.

    PTM databases

    PhosphoSiteiP09922.

    Expressioni

    Inductioni

    By type I and type III interferons.1 Publication

    Gene expression databases

    CleanExiMM_MX1.
    GenevestigatoriP09922.

    Interactioni

    Subunit structurei

    Homooligomer. Oligomerizes into multimeric filamentous or ring-like structures by virtue of its stalk domain. Oligomerization is critical for GTPase activity, protein stability, and recognition of viral target structures By similarity. Interacts with TRPC1, TRPC3, TRPC4, TRPC5, TRPC6 and TRPC7 By similarity. Interacts with HSPA5 By similarity. Interacts with TUBB/TUBB5 By similarity. Interacts with DDX39A and DDX39B.By similarity1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP09922.
    SMRiP09922. Positions 11-629.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini33 – 306274Dynamin-type GAdd
    BLAST
    Domaini543 – 63189GEDPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni35 – 306272GTPase domainBy similarityAdd
    BLAST
    Regioni307 – 33226Bundle signaling element (BSE)By similarityAdd
    BLAST
    Regioni332 – 499168Middle domainBy similarityAdd
    BLAST
    Regioni333 – 601269StalkBy similarityAdd
    BLAST
    Regioni520 – 5223Critical for lipid-bindingBy similarity

    Domaini

    The C-terminal GTPase effector domain (GED) is involved in oligomerization and viral target recognition.By similarity
    The middle domain mediates self-assembly and oligomerization.By similarity

    Sequence similaritiesi

    Contains 1 GED domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOVERGENiHBG008788.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR000375. Dynamin_central.
    IPR001401. Dynamin_GTPase.
    IPR019762. Dynamin_GTPase_CS.
    IPR022812. Dynamin_SF.
    IPR003130. GED.
    IPR020850. GTPase_effector_domain_GED.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR11566. PTHR11566. 1 hit.
    PfamiPF01031. Dynamin_M. 1 hit.
    PF00350. Dynamin_N. 1 hit.
    PF02212. GED. 1 hit.
    [Graphical view]
    PRINTSiPR00195. DYNAMIN.
    SMARTiSM00053. DYNc. 1 hit.
    SM00302. GED. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00410. G_DYNAMIN_1. 1 hit.
    PS51718. G_DYNAMIN_2. 1 hit.
    PS51388. GED. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P09922-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDSVNNLCRH YEEKVRPCID LIDTLRALGV EQDLALPAIA VIGDQSSGKS    50
    SVLEALSGVA LPRGSGIVTR CPLVLKLRKL KEGEEWRGKV SYDDIEVELS 100
    DPSEVEEAIN KGQNFIAGVG LGISDKLISL DVSSPNVPDL TLIDLPGITR 150
    VAVGNQPADI GRQIKRLIKT YIQKQETINL VVVPSNVDIA TTEALSMAQE 200
    VDPEGDRTIG VLTKPDLVDR GAEGKVLDVM RNLVYPLKKG YMIVKCRGQQ 250
    DIQEQLSLTE AFQKEQVFFK DHSYFSILLE DGKATVPCLA ERLTEELTSH 300
    ICKSLPLLED QINSSHQSAS EELQKYGADI PEDDRTRMSF LVNKISAFNR 350
    NIMNLIQAQE TVSEGDSRLF TKLRNEFLAW DDHIEEYFKK DSPEVQSKMK 400
    EFENQYRGRE LPGFVDYKAF ESIIKKRVKA LEESAVNMLR RVTKMVQTAF 450
    VKILSNDFGD FLNLCCTAKS KIKEIRLNQE KEAENLIRLH FQMEQIVYCQ 500
    DQVYKETLKT IREKEAEKEK TKALINPATF QNNSQFPQKG LTTTEMTQHL 550
    KAYYQECRRN IGRQIPLIIQ YFILKTFGEE IEKMMLQLLQ DTSKCSWFLE 600
    EQSDTREKKK FLKRRLLRLD EARQKLAKFS D 631
    Length:631
    Mass (Da):72,038
    Last modified:July 1, 1989 - v1
    Checksum:i7F0827668D190D68
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M12279 mRNA. Translation: AAA39776.1.
    M21117
    , M21105, M21106, M21107, M21108, M21109, M21110, M21111, M21112, M21113, M21114, M21115, M21116 Genomic DNA. Translation: AAA39777.1.
    BC011113 mRNA. Translation: AAH11113.1.
    PIRiA31203.
    RefSeqiNP_034976.1. NM_010846.1.
    UniGeneiMm.33996.

    Genome annotation databases

    GeneIDi17857.
    KEGGimmu:17857.
    UCSCiuc008adf.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M12279 mRNA. Translation: AAA39776.1 .
    M21117
    , M21105 , M21106 , M21107 , M21108 , M21109 , M21110 , M21111 , M21112 , M21113 , M21114 , M21115 , M21116 Genomic DNA. Translation: AAA39777.1 .
    BC011113 mRNA. Translation: AAH11113.1 .
    PIRi A31203.
    RefSeqi NP_034976.1. NM_010846.1.
    UniGenei Mm.33996.

    3D structure databases

    ProteinModelPortali P09922.
    SMRi P09922. Positions 11-629.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P09922.

    Proteomic databases

    PRIDEi P09922.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 17857.
    KEGGi mmu:17857.
    UCSCi uc008adf.1. mouse.

    Organism-specific databases

    CTDi 4599.
    MGIi MGI:97243. Mx1.

    Phylogenomic databases

    HOVERGENi HBG008788.

    Miscellaneous databases

    NextBioi 292605.
    PROi P09922.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_MX1.
    Genevestigatori P09922.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR000375. Dynamin_central.
    IPR001401. Dynamin_GTPase.
    IPR019762. Dynamin_GTPase_CS.
    IPR022812. Dynamin_SF.
    IPR003130. GED.
    IPR020850. GTPase_effector_domain_GED.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR11566. PTHR11566. 1 hit.
    Pfami PF01031. Dynamin_M. 1 hit.
    PF00350. Dynamin_N. 1 hit.
    PF02212. GED. 1 hit.
    [Graphical view ]
    PRINTSi PR00195. DYNAMIN.
    SMARTi SM00053. DYNc. 1 hit.
    SM00302. GED. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00410. G_DYNAMIN_1. 1 hit.
    PS51718. G_DYNAMIN_2. 1 hit.
    PS51388. GED. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mx protein: constitutive expression in 3T3 cells transformed with cloned Mx cDNA confers selective resistance to influenza virus."
      Staeheli P., Haller O., Boll W., Lindenmann J., Weissmann C.
      Cell 44:147-158(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Organization of the murine Mx gene and characterization of its interferon- and virus-inducible promoter."
      Hug H., Costas M., Staeheli P., Aebi M., Weissmann C.
      Mol. Cell. Biol. 8:3065-3079(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary gland.
    4. "A functional GTP-binding motif is necessary for antiviral activity of Mx proteins."
      Pitossi F., Blank A., Schroeder A., Schwarz A., Huessi P., Schwemmle M., Pavlovic J., Staeheli P.
      J. Virol. 67:6726-6732(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF SER-47 AND LYS-49.
    5. "The Mx1 gene protects mice against the pandemic 1918 and highly lethal human H5N1 influenza viruses."
      Tumpey T.M., Szretter K.J., Van Hoeven N., Katz J.M., Kochs G., Haller O., Garcia-Sastre A., Staeheli P.
      J. Virol. 81:10818-10821(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN RESISTANCE TO INFLUENZA VIRUS.
    6. "The Mx GTPase family of interferon-induced antiviral proteins."
      Haller O., Stertz S., Kochs G.
      Microbes Infect. 9:1636-1643(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW, INDUCTION.
    7. "Interferon-induced antiviral protein MxA interacts with the cellular RNA helicases UAP56 and URH49."
      Wisskirchen C., Ludersdorfer T.H., Mueller D.A., Moritz E., Pavlovic J.
      J. Biol. Chem. 286:34743-34751(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH DDX39A AND DDX39B.
    8. "Efficient influenza B virus propagation due to deficient interferon-induced antiviral activity in MDCK cells."
      Frensing T., Seitz C., Heynisch B., Patzina C., Kochs G., Reichl U.
      Vaccine 29:7125-7129(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Molecular signatures associated with Mx1-mediated resistance to highly pathogenic influenza virus infection: mechanisms of survival."
      Cilloniz C., Pantin-Jackwood M.J., Ni C., Carter V.S., Korth M.J., Swayne D.E., Tumpey T.M., Katze M.G.
      J. Virol. 86:2437-2446(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: RESISTANCE TO INFLUENZA VIRUS.

    Entry informationi

    Entry nameiMX1_MOUSE
    AccessioniPrimary (citable) accession number: P09922
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3