Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P09922 (MX1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Interferon-induced GTP-binding protein Mx1
Alternative name(s):
Influenza resistance protein
Myxoma resistance protein 1
Myxovirus resistance protein 1
Gene names
Name:Mx1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length631 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Interferon-induced dynamin-like GTPase with antiviral activity against influenza A virus, (IAV), influenza B virus (IBV) and Thogoto virus (THOV). Inhibits FLUAV by interfering with the process of primary transcription, probably by affecting the viral polymerase function. Ref.5 Ref.8

Subunit structure

Homooligomer. Oligomerizes into multimeric filamentous or ring-like structures by virtue of its stalk domain. Oligomerization is critical for GTPase activity, protein stability, and recognition of viral target structures By similarity. Interacts with TRPC1, TRPC3, TRPC4, TRPC5, TRPC6 and TRPC7 By similarity. Interacts with HSPA5 By similarity. Interacts with TUBB/TUBB5 By similarity. Interacts with DDX39A and DDX39B. Ref.7

Subcellular location

Nucleus Ref.7.

Induction

By type I and type III interferons. Ref.6

Domain

The C-terminal GTPase effector domain (GED) is involved in oligomerization and viral target recognition By similarity.

The middle domain mediates self-assembly and oligomerization By similarity.

Post-translational modification

ISGylated By similarity.

Sequence similarities

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.

Contains 1 dynamin-type G (guanine nucleotide-binding) domain.

Contains 1 GED domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 631631Interferon-induced GTP-binding protein Mx1
PRO_0000206593

Regions

Domain33 – 306274Dynamin-type G
Domain543 – 63189GED
Nucleotide binding43 – 508GTP Potential
Nucleotide binding144 – 1485GTP Potential
Nucleotide binding213 – 2164GTP Potential
Region35 – 306272GTPase domain By similarity
Region307 – 33226Bundle signaling element (BSE) By similarity
Region332 – 499168Middle domain By similarity
Region333 – 601269Stalk By similarity
Region520 – 5223Critical for lipid-binding By similarity

Experimental info

Mutagenesis471S → C: No effect on viral infection. Ref.4
Mutagenesis491K → A or M: Loss of protection against viral infection. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P09922 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 7F0827668D190D68

FASTA63172,038
        10         20         30         40         50         60 
MDSVNNLCRH YEEKVRPCID LIDTLRALGV EQDLALPAIA VIGDQSSGKS SVLEALSGVA 

        70         80         90        100        110        120 
LPRGSGIVTR CPLVLKLRKL KEGEEWRGKV SYDDIEVELS DPSEVEEAIN KGQNFIAGVG 

       130        140        150        160        170        180 
LGISDKLISL DVSSPNVPDL TLIDLPGITR VAVGNQPADI GRQIKRLIKT YIQKQETINL 

       190        200        210        220        230        240 
VVVPSNVDIA TTEALSMAQE VDPEGDRTIG VLTKPDLVDR GAEGKVLDVM RNLVYPLKKG 

       250        260        270        280        290        300 
YMIVKCRGQQ DIQEQLSLTE AFQKEQVFFK DHSYFSILLE DGKATVPCLA ERLTEELTSH 

       310        320        330        340        350        360 
ICKSLPLLED QINSSHQSAS EELQKYGADI PEDDRTRMSF LVNKISAFNR NIMNLIQAQE 

       370        380        390        400        410        420 
TVSEGDSRLF TKLRNEFLAW DDHIEEYFKK DSPEVQSKMK EFENQYRGRE LPGFVDYKAF 

       430        440        450        460        470        480 
ESIIKKRVKA LEESAVNMLR RVTKMVQTAF VKILSNDFGD FLNLCCTAKS KIKEIRLNQE 

       490        500        510        520        530        540 
KEAENLIRLH FQMEQIVYCQ DQVYKETLKT IREKEAEKEK TKALINPATF QNNSQFPQKG 

       550        560        570        580        590        600 
LTTTEMTQHL KAYYQECRRN IGRQIPLIIQ YFILKTFGEE IEKMMLQLLQ DTSKCSWFLE 

       610        620        630 
EQSDTREKKK FLKRRLLRLD EARQKLAKFS D 

« Hide

References

« Hide 'large scale' references
[1]"Mx protein: constitutive expression in 3T3 cells transformed with cloned Mx cDNA confers selective resistance to influenza virus."
Staeheli P., Haller O., Boll W., Lindenmann J., Weissmann C.
Cell 44:147-158(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Organization of the murine Mx gene and characterization of its interferon- and virus-inducible promoter."
Hug H., Costas M., Staeheli P., Aebi M., Weissmann C.
Mol. Cell. Biol. 8:3065-3079(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Czech II.
Tissue: Mammary gland.
[4]"A functional GTP-binding motif is necessary for antiviral activity of Mx proteins."
Pitossi F., Blank A., Schroeder A., Schwarz A., Huessi P., Schwemmle M., Pavlovic J., Staeheli P.
J. Virol. 67:6726-6732(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF SER-47 AND LYS-49.
[5]"The Mx1 gene protects mice against the pandemic 1918 and highly lethal human H5N1 influenza viruses."
Tumpey T.M., Szretter K.J., Van Hoeven N., Katz J.M., Kochs G., Haller O., Garcia-Sastre A., Staeheli P.
J. Virol. 81:10818-10821(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN RESISTANCE TO INFLUENZA VIRUS.
[6]"The Mx GTPase family of interferon-induced antiviral proteins."
Haller O., Stertz S., Kochs G.
Microbes Infect. 9:1636-1643(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW, INDUCTION.
[7]"Interferon-induced antiviral protein MxA interacts with the cellular RNA helicases UAP56 and URH49."
Wisskirchen C., Ludersdorfer T.H., Mueller D.A., Moritz E., Pavlovic J.
J. Biol. Chem. 286:34743-34751(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH DDX39A AND DDX39B.
[8]"Efficient influenza B virus propagation due to deficient interferon-induced antiviral activity in MDCK cells."
Frensing T., Seitz C., Heynisch B., Patzina C., Kochs G., Reichl U.
Vaccine 29:7125-7129(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Molecular signatures associated with Mx1-mediated resistance to highly pathogenic influenza virus infection: mechanisms of survival."
Cilloniz C., Pantin-Jackwood M.J., Ni C., Carter V.S., Korth M.J., Swayne D.E., Tumpey T.M., Katze M.G.
J. Virol. 86:2437-2446(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: RESISTANCE TO INFLUENZA VIRUS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M12279 mRNA. Translation: AAA39776.1.
M21117 expand/collapse EMBL AC list , M21105, M21106, M21107, M21108, M21109, M21110, M21111, M21112, M21113, M21114, M21115, M21116 Genomic DNA. Translation: AAA39777.1.
BC011113 mRNA. Translation: AAH11113.1.
PIRA31203.
RefSeqNP_034976.1. NM_010846.1.
UniGeneMm.33996.

3D structure databases

ProteinModelPortalP09922.
SMRP09922. Positions 11-629.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP09922.

Proteomic databases

PRIDEP09922.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID17857.
KEGGmmu:17857.
UCSCuc008adf.1. mouse.

Organism-specific databases

CTD4599.
MGIMGI:97243. Mx1.

Phylogenomic databases

HOVERGENHBG008788.

Gene expression databases

CleanExMM_MX1.
GenevestigatorP09922.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR003130. GED.
IPR020850. GTPase_effector_domain_GED.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR11566. PTHR11566. 1 hit.
PfamPF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
[Graphical view]
PRINTSPR00195. DYNAMIN.
SMARTSM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00410. G_DYNAMIN_1. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
PS51388. GED. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio292605.
PROP09922.
SOURCESearch...

Entry information

Entry nameMX1_MOUSE
AccessionPrimary (citable) accession number: P09922
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: July 9, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot