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P09922

- MX1_MOUSE

UniProt

P09922 - MX1_MOUSE

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Protein

Interferon-induced GTP-binding protein Mx1

Gene

Mx1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Interferon-induced dynamin-like GTPase with antiviral activity against influenza A virus, (IAV), influenza B virus (IBV) and Thogoto virus (THOV). Inhibits FLUAV by interfering with the process of primary transcription, probably by affecting the viral polymerase function.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi43 – 508GTPSequence Analysis
Nucleotide bindingi144 – 1485GTPSequence Analysis
Nucleotide bindingi213 – 2164GTPSequence Analysis

GO - Molecular functioni

  1. GTPase activity Source: MGI
  2. GTP binding Source: MGI

GO - Biological processi

  1. defense response to virus Source: UniProtKB-KW
  2. GTP catabolic process Source: GOC
  3. innate immune response Source: UniProtKB
  4. response to virus Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon-induced GTP-binding protein Mx1
Alternative name(s):
Influenza resistance protein
Myxoma resistance protein 1
Myxovirus resistance protein 1
Gene namesi
Name:Mx1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:97243. Mx1.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi47 – 471S → C: No effect on viral infection. 1 Publication
Mutagenesisi49 – 491K → A or M: Loss of protection against viral infection. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 631631Interferon-induced GTP-binding protein Mx1PRO_0000206593Add
BLAST

Post-translational modificationi

ISGylated.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PRIDEiP09922.

PTM databases

PhosphoSiteiP09922.

Expressioni

Inductioni

By type I and type III interferons.1 Publication

Gene expression databases

CleanExiMM_MX1.
GenevestigatoriP09922.

Interactioni

Subunit structurei

Homooligomer. Oligomerizes into multimeric filamentous or ring-like structures by virtue of its stalk domain. Oligomerization is critical for GTPase activity, protein stability, and recognition of viral target structures (By similarity). Interacts with TRPC1, TRPC3, TRPC4, TRPC5, TRPC6 and TRPC7 (By similarity). Interacts with HSPA5 (By similarity). Interacts with TUBB/TUBB5 (By similarity). Interacts with DDX39A and DDX39B.By similarity1 Publication

Structurei

3D structure databases

ProteinModelPortaliP09922.
SMRiP09922. Positions 11-629.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 306274Dynamin-type GAdd
BLAST
Domaini543 – 63189GEDPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni35 – 306272GTPase domainBy similarityAdd
BLAST
Regioni307 – 33226Bundle signaling element (BSE)By similarityAdd
BLAST
Regioni332 – 499168Middle domainBy similarityAdd
BLAST
Regioni333 – 601269StalkBy similarityAdd
BLAST
Regioni520 – 5223Critical for lipid-bindingBy similarity

Domaini

The C-terminal GTPase effector domain (GED) is involved in oligomerization and viral target recognition.By similarity
The middle domain mediates self-assembly and oligomerization.By similarity

Sequence similaritiesi

Contains 1 GED domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG008788.
InParanoidiP09922.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR003130. GED.
IPR020850. GTPase_effector_domain_GED.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11566. PTHR11566. 1 hit.
PfamiPF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
[Graphical view]
PRINTSiPR00195. DYNAMIN.
SMARTiSM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00410. G_DYNAMIN_1. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
PS51388. GED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09922-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDSVNNLCRH YEEKVRPCID LIDTLRALGV EQDLALPAIA VIGDQSSGKS
60 70 80 90 100
SVLEALSGVA LPRGSGIVTR CPLVLKLRKL KEGEEWRGKV SYDDIEVELS
110 120 130 140 150
DPSEVEEAIN KGQNFIAGVG LGISDKLISL DVSSPNVPDL TLIDLPGITR
160 170 180 190 200
VAVGNQPADI GRQIKRLIKT YIQKQETINL VVVPSNVDIA TTEALSMAQE
210 220 230 240 250
VDPEGDRTIG VLTKPDLVDR GAEGKVLDVM RNLVYPLKKG YMIVKCRGQQ
260 270 280 290 300
DIQEQLSLTE AFQKEQVFFK DHSYFSILLE DGKATVPCLA ERLTEELTSH
310 320 330 340 350
ICKSLPLLED QINSSHQSAS EELQKYGADI PEDDRTRMSF LVNKISAFNR
360 370 380 390 400
NIMNLIQAQE TVSEGDSRLF TKLRNEFLAW DDHIEEYFKK DSPEVQSKMK
410 420 430 440 450
EFENQYRGRE LPGFVDYKAF ESIIKKRVKA LEESAVNMLR RVTKMVQTAF
460 470 480 490 500
VKILSNDFGD FLNLCCTAKS KIKEIRLNQE KEAENLIRLH FQMEQIVYCQ
510 520 530 540 550
DQVYKETLKT IREKEAEKEK TKALINPATF QNNSQFPQKG LTTTEMTQHL
560 570 580 590 600
KAYYQECRRN IGRQIPLIIQ YFILKTFGEE IEKMMLQLLQ DTSKCSWFLE
610 620 630
EQSDTREKKK FLKRRLLRLD EARQKLAKFS D
Length:631
Mass (Da):72,038
Last modified:July 1, 1989 - v1
Checksum:i7F0827668D190D68
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M12279 mRNA. Translation: AAA39776.1.
M21117
, M21105, M21106, M21107, M21108, M21109, M21110, M21111, M21112, M21113, M21114, M21115, M21116 Genomic DNA. Translation: AAA39777.1.
BC011113 mRNA. Translation: AAH11113.1.
PIRiA31203.
RefSeqiNP_034976.1. NM_010846.1.
UniGeneiMm.33996.

Genome annotation databases

GeneIDi17857.
KEGGimmu:17857.
UCSCiuc008adf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M12279 mRNA. Translation: AAA39776.1 .
M21117
, M21105 , M21106 , M21107 , M21108 , M21109 , M21110 , M21111 , M21112 , M21113 , M21114 , M21115 , M21116 Genomic DNA. Translation: AAA39777.1 .
BC011113 mRNA. Translation: AAH11113.1 .
PIRi A31203.
RefSeqi NP_034976.1. NM_010846.1.
UniGenei Mm.33996.

3D structure databases

ProteinModelPortali P09922.
SMRi P09922. Positions 11-629.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P09922.

Proteomic databases

PRIDEi P09922.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 17857.
KEGGi mmu:17857.
UCSCi uc008adf.1. mouse.

Organism-specific databases

CTDi 4599.
MGIi MGI:97243. Mx1.

Phylogenomic databases

HOVERGENi HBG008788.
InParanoidi P09922.

Miscellaneous databases

NextBioi 292605.
PROi P09922.
SOURCEi Search...

Gene expression databases

CleanExi MM_MX1.
Genevestigatori P09922.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR003130. GED.
IPR020850. GTPase_effector_domain_GED.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR11566. PTHR11566. 1 hit.
Pfami PF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
[Graphical view ]
PRINTSi PR00195. DYNAMIN.
SMARTi SM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00410. G_DYNAMIN_1. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
PS51388. GED. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mx protein: constitutive expression in 3T3 cells transformed with cloned Mx cDNA confers selective resistance to influenza virus."
    Staeheli P., Haller O., Boll W., Lindenmann J., Weissmann C.
    Cell 44:147-158(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Organization of the murine Mx gene and characterization of its interferon- and virus-inducible promoter."
    Hug H., Costas M., Staeheli P., Aebi M., Weissmann C.
    Mol. Cell. Biol. 8:3065-3079(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary gland.
  4. "A functional GTP-binding motif is necessary for antiviral activity of Mx proteins."
    Pitossi F., Blank A., Schroeder A., Schwarz A., Huessi P., Schwemmle M., Pavlovic J., Staeheli P.
    J. Virol. 67:6726-6732(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF SER-47 AND LYS-49.
  5. "The Mx1 gene protects mice against the pandemic 1918 and highly lethal human H5N1 influenza viruses."
    Tumpey T.M., Szretter K.J., Van Hoeven N., Katz J.M., Kochs G., Haller O., Garcia-Sastre A., Staeheli P.
    J. Virol. 81:10818-10821(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN RESISTANCE TO INFLUENZA VIRUS.
  6. "The Mx GTPase family of interferon-induced antiviral proteins."
    Haller O., Stertz S., Kochs G.
    Microbes Infect. 9:1636-1643(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW, INDUCTION.
  7. "Interferon-induced antiviral protein MxA interacts with the cellular RNA helicases UAP56 and URH49."
    Wisskirchen C., Ludersdorfer T.H., Mueller D.A., Moritz E., Pavlovic J.
    J. Biol. Chem. 286:34743-34751(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH DDX39A AND DDX39B.
  8. "Efficient influenza B virus propagation due to deficient interferon-induced antiviral activity in MDCK cells."
    Frensing T., Seitz C., Heynisch B., Patzina C., Kochs G., Reichl U.
    Vaccine 29:7125-7129(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Molecular signatures associated with Mx1-mediated resistance to highly pathogenic influenza virus infection: mechanisms of survival."
    Cilloniz C., Pantin-Jackwood M.J., Ni C., Carter V.S., Korth M.J., Swayne D.E., Tumpey T.M., Katze M.G.
    J. Virol. 86:2437-2446(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: RESISTANCE TO INFLUENZA VIRUS.

Entry informationi

Entry nameiMX1_MOUSE
AccessioniPrimary (citable) accession number: P09922
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 29, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3