P09922 (MX1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interferon-induced GTP-binding protein Mx1

Alternative name(s):
Influenza resistance protein
Myxoma resistance protein 1
Myxovirus resistance protein 1

Gene names

Name:

Mx1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	631 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Interferon-induced dynamin-like GTPase with antiviral activity against influenza A virus, (IAV), influenza B virus (IBV) and Thogoto virus (THOV). Inhibits FLUAV by interfering with the process of primary transcription, probably by affecting the viral polymerase function. Ref.5 Ref.8
Subunit structure	Homooligomer. Oligomerizes into multimeric filamentous or ring-like structures by virtue of its stalk domain. Oligomerization is critical for GTPase activity, protein stability, and recognition of viral target structures By similarity. Interacts with TRPC1, TRPC3, TRPC4, TRPC5, TRPC6 and TRPC7 By similarity. Interacts with HSPA5 By similarity. Interacts with TUBB/TUBB5 By similarity. Interacts with DDX39A and DDX39B. Ref.7
Subcellular location	Nucleus Ref.7.
Induction	By type I and type III interferons. Ref.6
Domain	The C-terminal GTPase effector domain (GED) is involved in oligomerization and viral target recognition By similarity. The middle domain mediates self-assembly and oligomerization By similarity.
Sequence similarities	Belongs to the dynamin family. Contains 1 GED domain.

Ontologies

Keywords
Biological process	Antiviral defense Immunity Innate immunity
Cellular component	Nucleus
Ligand	GTP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response to virus Inferred from electronic annotation. Source: UniProtKB-KW innate immune response Inferred from direct assay Ref.8. Source: UniProtKB response to virus Inferred from mutant phenotype Ref.8. Source: UniProtKB
Cellular_component	nucleus Inferred from direct assay Ref.7. Source: UniProtKB
Molecular_function	GTP binding Traceable author statement PubMed 11932237. Source: MGI GTPase activity Traceable author statement PubMed 11932237. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 631	631	Interferon-induced GTP-binding protein Mx1		PRO_0000206593
Regions
	Domain	543 – 631	89	GED
	Nucleotide binding	43 – 50	8	GTP Potential
	Nucleotide binding	144 – 148	5	GTP Potential
	Nucleotide binding	213 – 216	4	GTP Potential
	Region	35 – 306	272	GTPase domain By similarity
	Region	307 – 332	26	Bundle signaling element (BSE) By similarity
	Region	332 – 499	168	Middle domain By similarity
	Region	333 – 601	269	Stalk By similarity
	Region	520 – 522	3	Critical for lipid-binding By similarity
Experimental info
	Mutagenesis	47	1	S → C: No effect on viral infection. Ref.4
	Mutagenesis	49	1	K → A or M: Loss of protection against viral infection. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P09922 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 7F0827668D190D68	FASTA	631	72,038
	10 20 30 40 50 60 MDSVNNLCRH YEEKVRPCID LIDTLRALGV EQDLALPAIA VIGDQSSGKS SVLEALSGVA 70 80 90 100 110 120 LPRGSGIVTR CPLVLKLRKL KEGEEWRGKV SYDDIEVELS DPSEVEEAIN KGQNFIAGVG 130 140 150 160 170 180 LGISDKLISL DVSSPNVPDL TLIDLPGITR VAVGNQPADI GRQIKRLIKT YIQKQETINL 190 200 210 220 230 240 VVVPSNVDIA TTEALSMAQE VDPEGDRTIG VLTKPDLVDR GAEGKVLDVM RNLVYPLKKG 250 260 270 280 290 300 YMIVKCRGQQ DIQEQLSLTE AFQKEQVFFK DHSYFSILLE DGKATVPCLA ERLTEELTSH 310 320 330 340 350 360 ICKSLPLLED QINSSHQSAS EELQKYGADI PEDDRTRMSF LVNKISAFNR NIMNLIQAQE 370 380 390 400 410 420 TVSEGDSRLF TKLRNEFLAW DDHIEEYFKK DSPEVQSKMK EFENQYRGRE LPGFVDYKAF 430 440 450 460 470 480 ESIIKKRVKA LEESAVNMLR RVTKMVQTAF VKILSNDFGD FLNLCCTAKS KIKEIRLNQE 490 500 510 520 530 540 KEAENLIRLH FQMEQIVYCQ DQVYKETLKT IREKEAEKEK TKALINPATF QNNSQFPQKG 550 560 570 580 590 600 LTTTEMTQHL KAYYQECRRN IGRQIPLIIQ YFILKTFGEE IEKMMLQLLQ DTSKCSWFLE 610 620 630 EQSDTREKKK FLKRRLLRLD EARQKLAKFS D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mx protein: constitutive expression in 3T3 cells transformed with cloned Mx cDNA confers selective resistance to influenza virus." Staeheli P., Haller O., Boll W., Lindenmann J., Weissmann C. Cell 44:147-158(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Organization of the murine Mx gene and characterization of its interferon- and virus-inducible promoter." Hug H., Costas M., Staeheli P., Aebi M., Weissmann C. Mol. Cell. Biol. 8:3065-3079(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary gland.
[4]	"A functional GTP-binding motif is necessary for antiviral activity of Mx proteins." Pitossi F., Blank A., Schroeder A., Schwarz A., Huessi P., Schwemmle M., Pavlovic J., Staeheli P. J. Virol. 67:6726-6732(1993) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF SER-47 AND LYS-49.
[5]	"The Mx1 gene protects mice against the pandemic 1918 and highly lethal human H5N1 influenza viruses." Tumpey T.M., Szretter K.J., Van Hoeven N., Katz J.M., Kochs G., Haller O., Garcia-Sastre A., Staeheli P. J. Virol. 81:10818-10821(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN RESISTANCE TO INFLUENZA VIRUS.
[6]	"The Mx GTPase family of interferon-induced antiviral proteins." Haller O., Stertz S., Kochs G. Microbes Infect. 9:1636-1643(2007) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW, INDUCTION.
[7]	"Interferon-induced antiviral protein MxA interacts with the cellular RNA helicases UAP56 and URH49." Wisskirchen C., Ludersdorfer T.H., Mueller D.A., Moritz E., Pavlovic J. J. Biol. Chem. 286:34743-34751(2011) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH DDX39A AND DDX39B.
[8]	"Efficient influenza B virus propagation due to deficient interferon-induced antiviral activity in MDCK cells." Frensing T., Seitz C., Heynisch B., Patzina C., Kochs G., Reichl U. Vaccine 29:7125-7129(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"Molecular signatures associated with Mx1-mediated resistance to highly pathogenic influenza virus infection: mechanisms of survival." Cilloniz C., Pantin-Jackwood M.J., Ni C., Carter V.S., Korth M.J., Swayne D.E., Tumpey T.M., Katze M.G. J. Virol. 86:2437-2446(2012) [PubMed] [Europe PMC] [Abstract] Cited for: RESISTANCE TO INFLUENZA VIRUS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M12279 mRNA. Translation: AAA39776.1. M21117 , M21105, M21106, M21107, M21108, M21109, M21110, M21111, M21112, M21113, M21114, M21115, M21116 Genomic DNA. Translation: AAA39777.1. BC011113 mRNA. Translation: AAH11113.1.
IPI	IPI00112518.
PIR	A31203.
RefSeq	NP_034976.1. NM_010846.1.
UniGene	Mm.33996.
3D structure databases
ProteinModelPortal	P09922.
SMR	P09922. Positions 11-629.
ModBase	Search...
PTM databases
PhosphoSite	P09922.
Proteomic databases
PRIDE	P09922.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	17857.
KEGG	mmu:17857.
Organism-specific databases
CTD	4599.
MGI	MGI:97243. Mx1.
Phylogenomic databases
HOVERGEN	HBG008788.
Gene expression databases
CleanEx	MM_MX1.
Genevestigator	P09922.
Family and domain databases
InterPro	IPR022812. Dynamin. IPR000375. Dynamin_central. IPR001401. Dynamin_GTPase. IPR019762. Dynamin_GTPase_CS. IPR003130. GED. IPR020850. GTPase_effector_domain_GED. IPR015577. Interferon-induced_Mx. [Graphical view]
PANTHER	PTHR11566. PTHR11566. 1 hit. PTHR11566:SF13. PTHR11566:SF13. 1 hit.
Pfam	PF01031. Dynamin_M. 1 hit. PF00350. Dynamin_N. 1 hit. PF02212. GED. 1 hit. [Graphical view]
PRINTS	PR00195. DYNAMIN.
SMART	SM00053. DYNc. 1 hit. SM00302. GED. 1 hit. [Graphical view]
PROSITE	PS00410. DYNAMIN. 1 hit. PS51388. GED. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	292605.
SOURCE	Search...

Entry information

Entry name

MX1_MOUSE

Accession

Primary (citable) accession number: P09922

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program