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Protein

Interferon-induced GTP-binding protein Mx1

Gene

Mx1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interferon-induced dynamin-like GTPase with antiviral activity against influenza A virus, (IAV), influenza B virus (IBV) and Thogoto virus (THOV). Inhibits FLUAV by interfering with the process of primary transcription, probably by affecting the viral polymerase function.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi43 – 50GTPSequence analysis8
Nucleotide bindingi144 – 148GTPSequence analysis5
Nucleotide bindingi213 – 216GTPSequence analysis4

GO - Molecular functioni

  • GTPase activity Source: MGI
  • GTP binding Source: MGI
  • microtubule binding Source: GO_Central

GO - Biological processi

  • defense response to virus Source: GO_Central
  • innate immune response Source: UniProtKB
  • organelle fission Source: GO_Central
  • response to virus Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon-induced GTP-binding protein Mx1
Alternative name(s):
Influenza resistance protein
Myxoma resistance protein 1
Myxovirus resistance protein 1
Gene namesi
Name:Mx1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97243. Mx1.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication
  • Endoplasmic reticulum membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
  • Cytoplasmperinuclear region By similarity

  • Note: Binds preferentially to negatively charged phospholipids. Colocalizes with CCHFV protein N in the perinuclear region.By similarity

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi47S → C: No effect on viral infection. 1 Publication1
Mutagenesisi49K → A or M: Loss of protection against viral infection. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002065931 – 631Interferon-induced GTP-binding protein Mx1Add BLAST631

Post-translational modificationi

ISGylated.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PRIDEiP09922.

PTM databases

iPTMnetiP09922.
PhosphoSitePlusiP09922.

Expressioni

Inductioni

By type I and type III interferons.1 Publication

Gene expression databases

CleanExiMM_MX1.

Interactioni

Subunit structurei

Homooligomer. Oligomerizes into multimeric filamentous or ring-like structures by virtue of its stalk domain. Oligomerization is critical for GTPase activity, protein stability, and recognition of viral target structures (By similarity). Interacts with TRPC1, TRPC3, TRPC4, TRPC5, TRPC6 and TRPC7 (By similarity). Interacts with HSPA5 (By similarity). Interacts with TUBB/TUBB5 (By similarity). Interacts with DDX39A and DDX39B.By similarity1 Publication

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliP09922.
SMRiP09922.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 306Dynamin-type GAdd BLAST274
Domaini543 – 631GEDPROSITE-ProRule annotationAdd BLAST89

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 306GTPase domainBy similarityAdd BLAST272
Regioni307 – 332Bundle signaling element (BSE)By similarityAdd BLAST26
Regioni332 – 499Middle domainBy similarityAdd BLAST168
Regioni333 – 601StalkBy similarityAdd BLAST269
Regioni520 – 522Critical for lipid-bindingBy similarity3

Domaini

The C-terminal GTPase effector domain (GED) is involved in oligomerization and viral target recognition.By similarity
The middle domain mediates self-assembly and oligomerization.By similarity

Sequence similaritiesi

Contains 1 GED domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG008788.
InParanoidiP09922.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR030381. G_DYNAMIN_dom.
IPR003130. GED.
IPR020850. GED_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
[Graphical view]
PRINTSiPR00195. DYNAMIN.
SMARTiSM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00410. G_DYNAMIN_1. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
PS51388. GED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09922-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSVNNLCRH YEEKVRPCID LIDTLRALGV EQDLALPAIA VIGDQSSGKS
60 70 80 90 100
SVLEALSGVA LPRGSGIVTR CPLVLKLRKL KEGEEWRGKV SYDDIEVELS
110 120 130 140 150
DPSEVEEAIN KGQNFIAGVG LGISDKLISL DVSSPNVPDL TLIDLPGITR
160 170 180 190 200
VAVGNQPADI GRQIKRLIKT YIQKQETINL VVVPSNVDIA TTEALSMAQE
210 220 230 240 250
VDPEGDRTIG VLTKPDLVDR GAEGKVLDVM RNLVYPLKKG YMIVKCRGQQ
260 270 280 290 300
DIQEQLSLTE AFQKEQVFFK DHSYFSILLE DGKATVPCLA ERLTEELTSH
310 320 330 340 350
ICKSLPLLED QINSSHQSAS EELQKYGADI PEDDRTRMSF LVNKISAFNR
360 370 380 390 400
NIMNLIQAQE TVSEGDSRLF TKLRNEFLAW DDHIEEYFKK DSPEVQSKMK
410 420 430 440 450
EFENQYRGRE LPGFVDYKAF ESIIKKRVKA LEESAVNMLR RVTKMVQTAF
460 470 480 490 500
VKILSNDFGD FLNLCCTAKS KIKEIRLNQE KEAENLIRLH FQMEQIVYCQ
510 520 530 540 550
DQVYKETLKT IREKEAEKEK TKALINPATF QNNSQFPQKG LTTTEMTQHL
560 570 580 590 600
KAYYQECRRN IGRQIPLIIQ YFILKTFGEE IEKMMLQLLQ DTSKCSWFLE
610 620 630
EQSDTREKKK FLKRRLLRLD EARQKLAKFS D
Length:631
Mass (Da):72,038
Last modified:July 1, 1989 - v1
Checksum:i7F0827668D190D68
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12279 mRNA. Translation: AAA39776.1.
M21117
, M21105, M21106, M21107, M21108, M21109, M21110, M21111, M21112, M21113, M21114, M21115, M21116 Genomic DNA. Translation: AAA39777.1.
BC011113 mRNA. Translation: AAH11113.1.
PIRiA31203.
RefSeqiNP_034976.1. NM_010846.1.
UniGeneiMm.33996.

Genome annotation databases

GeneIDi17857.
KEGGimmu:17857.
UCSCiuc008adf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12279 mRNA. Translation: AAA39776.1.
M21117
, M21105, M21106, M21107, M21108, M21109, M21110, M21111, M21112, M21113, M21114, M21115, M21116 Genomic DNA. Translation: AAA39777.1.
BC011113 mRNA. Translation: AAH11113.1.
PIRiA31203.
RefSeqiNP_034976.1. NM_010846.1.
UniGeneiMm.33996.

3D structure databases

ProteinModelPortaliP09922.
SMRiP09922.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiP09922.
PhosphoSitePlusiP09922.

Proteomic databases

PRIDEiP09922.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi17857.
KEGGimmu:17857.
UCSCiuc008adf.1. mouse.

Organism-specific databases

CTDi4599.
MGIiMGI:97243. Mx1.

Phylogenomic databases

HOVERGENiHBG008788.
InParanoidiP09922.

Miscellaneous databases

PROiP09922.
SOURCEiSearch...

Gene expression databases

CleanExiMM_MX1.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR030381. G_DYNAMIN_dom.
IPR003130. GED.
IPR020850. GED_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
[Graphical view]
PRINTSiPR00195. DYNAMIN.
SMARTiSM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00410. G_DYNAMIN_1. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
PS51388. GED. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMX1_MOUSE
AccessioniPrimary (citable) accession number: P09922
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 2, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.