P09920 (CSF3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Granulocyte colony-stimulating factor
Short name=G-CSF

Gene names

Name:	Csf3
Synonyms:	Csfg

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Subunit structure	Monomer.
Subcellular location	Secreted.
Post-translational modification	O-glycosylated By similarity.
Sequence similarities	Belongs to the IL-6 superfamily.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Cytokine Growth factor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to lipopolysaccharide Inferred from electronic annotation. Source: Compara granulocyte differentiation Non-traceable author statement PubMed 15664994. Source: UniProtKB immune response Inferred from electronic annotation. Source: InterPro negative regulation of neuron death Inferred from electronic annotation. Source: Compara positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cytokine activity Non-traceable author statement PubMed 15664994. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30
	Chain	31 – 208	178	Granulocyte colony-stimulating factor		PRO_0000015571
Amino acid modifications
	Glycosylation	169	1	O-linked (GalNAc...) By similarity
	Disulfide bond	72 ↔ 78		By similarity
	Disulfide bond	100 ↔ 110		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P09920 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 0BF3622135C906DB	FASTA	208	22,421
	10 20 30 40 50 60 MAQLSAQRRM KLMALQLLLW QSALWSGREA VPLVTVSALP PSLPLPRSFL LKSLEQVRKI 70 80 90 100 110 120 QASGSVLLEQ LCATYKLCHP EELVLLGHSL GIPKASLSGC SSQALQQTQC LSQLHSGLCL 130 140 150 160 170 180 YQGLLQALSG ISPALAPTLD LLQLDVANFA TTIWQQMENL GVAPTVQPTQ SAMPAFTSAF 190 200 QRRAGGVLAI SYLQGFLETA RLALHHLA

References

[1]	"Isolation and characterization of the cDNA for murine granulocyte colony-stimulating factor." Tsuchiya M., Asano S., Kaziro Y., Nagata S. Proc. Natl. Acad. Sci. U.S.A. 83:7633-7637(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The chromosomal gene structure for murine granulocyte colony-stimulating factor." Tsuchiya M., Kaziro Y., Nagata S. Eur. J. Biochem. 165:7-12(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Structural studies on the murine granulocyte colony-stimulating factor." Simpson R.J., Nice E.C., Nicola N.A. Biol. Chem. Hoppe-Seyler 368:1327-1331(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M13926 mRNA. Translation: AAA37672.1. X05402 Genomic DNA. Translation: CAA28986.1.
IPI	IPI00112515.
PIR	A26496. A29536.
RefSeq	NP_034101.1. NM_009971.1.
UniGene	Mm.1238.
3D structure databases
ProteinModelPortal	P09920.
SMR	P09920. Positions 45-208.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6118N.
PTM databases
PhosphoSite	P09920.
Proteomic databases
PRIDE	P09920.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000038886; ENSMUSP00000037762; ENSMUSG00000038067.
GeneID	12985.
KEGG	mmu:12985.
UCSC	uc007lgy.1. mouse.
Organism-specific databases
CTD	1440.
MGI	MGI:1339751. Csf3.
Phylogenomic databases
eggNOG	NOG47652.
HOGENOM	HOG000015371.
HOVERGEN	HBG005411.
InParanoid	P09920.
KO	K05423.
OMA	CHPEELV.
OrthoDB	EOG4SBDZV.
Gene expression databases
ArrayExpress	P09920.
Bgee	P09920.
CleanEx	MM_CSF3.
Genevestigator	P09920.
GermOnline	ENSMUSG00000038067. Mus musculus.
Family and domain databases
Gene3D	1.20.1250.10. 1 hit.
InterPro	IPR009079. 4_helix_cytokine-like_core. IPR012351. 4_helix_cytokine_core. IPR003573. IL6_MGF_GCSF. [Graphical view]
Pfam	PF00489. IL6. 1 hit. [Graphical view]
PIRSF	PIRSF001935. IL6_MGF_GCSF. 1 hit.
PRINTS	PR00433. IL6GCSFMGF.
SMART	SM00126. IL6. 1 hit. [Graphical view]
SUPFAM	SSF47266. 4_helix_cytokine. 1 hit.
PROSITE	PS00254. INTERLEUKIN_6. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	282772.
SOURCE	Search...

Entry information

Entry name

CSF3_MOUSE

Accession

Primary (citable) accession number: P09920

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	May 29, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program