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Protein

Granulocyte colony-stimulating factor

Gene

Csf3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

GO - Molecular functioni

  1. cytokine activity Source: UniProtKB
  2. enzyme binding Source: MGI

GO - Biological processi

  1. cellular response to cytokine stimulus Source: MGI
  2. cellular response to lipopolysaccharide Source: Ensembl
  3. granulocyte differentiation Source: UniProtKB
  4. immune response Source: InterPro
  5. negative regulation of neuron death Source: Ensembl
  6. positive regulation of actin cytoskeleton reorganization Source: MGI
  7. positive regulation of actin filament polymerization Source: MGI
  8. positive regulation of cell proliferation Source: Ensembl
  9. positive regulation of myeloid cell differentiation Source: MGI
  10. positive regulation of peptidyl-serine phosphorylation Source: MGI
  11. positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
  12. positive regulation of phosphatidylinositol 3-kinase signaling Source: MGI
  13. positive regulation of protein binding Source: MGI
  14. positive regulation of protein kinase B signaling Source: MGI
  15. positive regulation of transcription factor import into nucleus Source: MGI
  16. positive regulation of transcription from RNA polymerase II promoter Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Growth factor

Names & Taxonomyi

Protein namesi
Recommended name:
Granulocyte colony-stimulating factor
Short name:
G-CSF
Gene namesi
Name:Csf3
Synonyms:Csfg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:1339751. Csf3.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Add
BLAST
Chaini31 – 208178Granulocyte colony-stimulating factorPRO_0000015571Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi72 ↔ 78By similarity
Disulfide bondi100 ↔ 110By similarity
Glycosylationi169 – 1691O-linked (GalNAc...)By similarity

Post-translational modificationi

O-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP09920.

PTM databases

PhosphoSiteiP09920.

Expressioni

Gene expression databases

BgeeiP09920.
CleanExiMM_CSF3.
ExpressionAtlasiP09920. baseline and differential.
GenevestigatoriP09920.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

DIPiDIP-6118N.

Structurei

3D structure databases

ProteinModelPortaliP09920.
SMRiP09920. Positions 45-208.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IL-6 superfamily.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG47652.
HOGENOMiHOG000015371.
HOVERGENiHBG005411.
InParanoidiP09920.
KOiK05423.
OMAiLWHSALW.
OrthoDBiEOG73JKX9.
PhylomeDBiP09920.
TreeFamiTF337698.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR003573. IL-6/IL-23/GCSF/MGF.
[Graphical view]
PfamiPF00489. IL6. 1 hit.
[Graphical view]
PIRSFiPIRSF001935. IL6_MGF_GCSF. 1 hit.
PRINTSiPR00433. IL6GCSFMGF.
SMARTiSM00126. IL6. 1 hit.
[Graphical view]
SUPFAMiSSF47266. SSF47266. 1 hit.
PROSITEiPS00254. INTERLEUKIN_6. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09920-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQLSAQRRM KLMALQLLLW QSALWSGREA VPLVTVSALP PSLPLPRSFL
60 70 80 90 100
LKSLEQVRKI QASGSVLLEQ LCATYKLCHP EELVLLGHSL GIPKASLSGC
110 120 130 140 150
SSQALQQTQC LSQLHSGLCL YQGLLQALSG ISPALAPTLD LLQLDVANFA
160 170 180 190 200
TTIWQQMENL GVAPTVQPTQ SAMPAFTSAF QRRAGGVLAI SYLQGFLETA

RLALHHLA
Length:208
Mass (Da):22,421
Last modified:July 1, 1989 - v1
Checksum:i0BF3622135C906DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13926 mRNA. Translation: AAA37672.1.
X05402 Genomic DNA. Translation: CAA28986.1.
CCDSiCCDS25360.1.
PIRiA29536. A26496.
RefSeqiNP_034101.1. NM_009971.1.
UniGeneiMm.1238.

Genome annotation databases

EnsembliENSMUST00000038886; ENSMUSP00000037762; ENSMUSG00000038067.
GeneIDi12985.
KEGGimmu:12985.
UCSCiuc007lgy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13926 mRNA. Translation: AAA37672.1.
X05402 Genomic DNA. Translation: CAA28986.1.
CCDSiCCDS25360.1.
PIRiA29536. A26496.
RefSeqiNP_034101.1. NM_009971.1.
UniGeneiMm.1238.

3D structure databases

ProteinModelPortaliP09920.
SMRiP09920. Positions 45-208.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6118N.

PTM databases

PhosphoSiteiP09920.

Proteomic databases

PRIDEiP09920.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038886; ENSMUSP00000037762; ENSMUSG00000038067.
GeneIDi12985.
KEGGimmu:12985.
UCSCiuc007lgy.1. mouse.

Organism-specific databases

CTDi1440.
MGIiMGI:1339751. Csf3.

Phylogenomic databases

eggNOGiNOG47652.
HOGENOMiHOG000015371.
HOVERGENiHBG005411.
InParanoidiP09920.
KOiK05423.
OMAiLWHSALW.
OrthoDBiEOG73JKX9.
PhylomeDBiP09920.
TreeFamiTF337698.

Miscellaneous databases

NextBioi282772.
PROiP09920.
SOURCEiSearch...

Gene expression databases

BgeeiP09920.
CleanExiMM_CSF3.
ExpressionAtlasiP09920. baseline and differential.
GenevestigatoriP09920.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR003573. IL-6/IL-23/GCSF/MGF.
[Graphical view]
PfamiPF00489. IL6. 1 hit.
[Graphical view]
PIRSFiPIRSF001935. IL6_MGF_GCSF. 1 hit.
PRINTSiPR00433. IL6GCSFMGF.
SMARTiSM00126. IL6. 1 hit.
[Graphical view]
SUPFAMiSSF47266. SSF47266. 1 hit.
PROSITEiPS00254. INTERLEUKIN_6. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and characterization of the cDNA for murine granulocyte colony-stimulating factor."
    Tsuchiya M., Asano S., Kaziro Y., Nagata S.
    Proc. Natl. Acad. Sci. U.S.A. 83:7633-7637(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The chromosomal gene structure for murine granulocyte colony-stimulating factor."
    Tsuchiya M., Kaziro Y., Nagata S.
    Eur. J. Biochem. 165:7-12(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Structural studies on the murine granulocyte colony-stimulating factor."
    Simpson R.J., Nice E.C., Nicola N.A.
    Biol. Chem. Hoppe-Seyler 368:1327-1331(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.

Entry informationi

Entry nameiCSF3_MOUSE
AccessioniPrimary (citable) accession number: P09920
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: March 4, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.