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Protein

Granulocyte colony-stimulating factor

Gene

CSF3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

GO - Molecular functioni

  • cytokine activity Source: UniProtKB
  • enzyme binding Source: UniProtKB
  • granulocyte colony-stimulating factor receptor binding Source: ProtInc
  • growth factor activity Source: GO_Central

GO - Biological processi

  • cellular response to cytokine stimulus Source: BHF-UCL
  • cellular response to lipopolysaccharide Source: Ensembl
  • cytokine-mediated signaling pathway Source: UniProtKB
  • granulocyte differentiation Source: UniProtKB
  • immune response Source: InterPro
  • multicellular organism development Source: ProtInc
  • negative regulation of neuron death Source: Ensembl
  • positive regulation of actin cytoskeleton reorganization Source: BHF-UCL
  • positive regulation of actin filament polymerization Source: BHF-UCL
  • positive regulation of cell proliferation Source: ProtInc
  • positive regulation of myeloid cell differentiation Source: GO_Central
  • positive regulation of peptidyl-serine phosphorylation Source: BHF-UCL
  • positive regulation of peptidyl-tyrosine phosphorylation Source: BHF-UCL
  • positive regulation of phosphatidylinositol 3-kinase signaling Source: BHF-UCL
  • positive regulation of protein binding Source: BHF-UCL
  • positive regulation of protein kinase B signaling Source: BHF-UCL
  • positive regulation of transcription factor import into nucleus Source: BHF-UCL
  • positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • response to ethanol Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Growth factor

Enzyme and pathway databases

BioCyciZFISH:ENSG00000108342-MONOMER.
ReactomeiR-HSA-449147. Signaling by Interleukins.
SignaLinkiP09919.
SIGNORiP09919.

Names & Taxonomyi

Protein namesi
Recommended name:
Granulocyte colony-stimulating factor
Short name:
G-CSF
Alternative name(s):
Pluripoietin
INN: Filgrastim
INN: Lenograstim
Gene namesi
Name:CSF3
Synonyms:C17orf33, GCSF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:2438. CSF3.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: Reactome
  • extracellular space Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Pharmaceutical usei

Available under the names Neupogen or Granulokine (Amgen/Roche) and Granocyte (Rhone-Poulenc). Used to treat neutropenia (a disorder characterized by an extremely low number of neutrophils in blood).

Organism-specific databases

DisGeNETi1440.
OpenTargetsiENSG00000108342.
PharmGKBiPA26941.

Protein family/group databases

Allergomei8367. Hom s G-CSF.

Polymorphism and mutation databases

BioMutaiCSF3.
DMDMi117564.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 291 PublicationAdd BLAST29
ChainiPRO_000001557030 – 207Granulocyte colony-stimulating factorAdd BLAST178

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi69 ↔ 75
Disulfide bondi97 ↔ 107
Glycosylationi166O-linked (GalNAc...)By similarity1

Post-translational modificationi

O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP09919.
PaxDbiP09919.
PeptideAtlasiP09919.
PRIDEiP09919.

PTM databases

iPTMnetiP09919.
PhosphoSitePlusiP09919.

Expressioni

Gene expression databases

BgeeiENSG00000108342.
CleanExiHS_CSF3.
ExpressionAtlasiP09919. baseline and differential.
GenevisibleiP09919. HS.

Organism-specific databases

HPAiCAB016131.
HPA001412.

Interactioni

Subunit structurei

Monomer.2 Publications

GO - Molecular functioni

  • cytokine activity Source: UniProtKB
  • enzyme binding Source: UniProtKB
  • granulocyte colony-stimulating factor receptor binding Source: ProtInc
  • growth factor activity Source: GO_Central

Protein-protein interaction databases

DIPiDIP-61120N.
STRINGi9606.ENSP00000225474.

Structurei

Secondary structure

1207
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi37 – 39Combined sources3
Helixi41 – 65Combined sources25
Helixi69 – 71Combined sources3
Helixi77 – 80Combined sources4
Helixi81 – 86Combined sources6
Beta strandi96 – 100Combined sources5
Helixi105 – 124Combined sources20
Turni125 – 127Combined sources3
Turni130 – 132Combined sources3
Helixi133 – 156Combined sources24
Beta strandi166 – 168Combined sources3
Helixi176 – 204Combined sources29

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CD9X-ray2.80A/C31-207[»]
1GNCNMR-A31-207[»]
1PGRX-ray3.50A/C/E/G31-207[»]
1RHGX-ray2.20A/B/C31-207[»]
2D9QX-ray2.80A31-207[»]
ProteinModelPortaliP09919.
SMRiP09919.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09919.

Family & Domainsi

Sequence similaritiesi

Belongs to the IL-6 superfamily.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IIM2. Eukaryota.
ENOG4111DA4. LUCA.
GeneTreeiENSGT00390000017328.
HOGENOMiHOG000015371.
HOVERGENiHBG005411.
InParanoidiP09919.
KOiK05423.
OMAiLWHSALW.
OrthoDBiEOG091G0NDU.
PhylomeDBiP09919.
TreeFamiTF337698.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR030474. IL-6/GCSF/MGF.
IPR030473. IL6/GCSF/MGF_CS.
[Graphical view]
PfamiPF16647. GCSF. 1 hit.
[Graphical view]
PIRSFiPIRSF001935. IL6_MGF_GCSF. 1 hit.
PRINTSiPR00433. IL6GCSFMGF.
SMARTiSM00126. IL6. 1 hit.
[Graphical view]
SUPFAMiSSF47266. SSF47266. 1 hit.
PROSITEiPS00254. INTERLEUKIN_6. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P09919-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGPATQSPM KLMALQLLLW HSALWTVQEA TPLGPASSLP QSFLLKCLEQ
60 70 80 90 100
VRKIQGDGAA LQEKLVSECA TYKLCHPEEL VLLGHSLGIP WAPLSSCPSQ
110 120 130 140 150
ALQLAGCLSQ LHSGLFLYQG LLQALEGISP ELGPTLDTLQ LDVADFATTI
160 170 180 190 200
WQQMEELGMA PALQPTQGAM PAFASAFQRR AGGVLVASHL QSFLEVSYRV

LRHLAQP
Length:207
Mass (Da):22,293
Last modified:July 1, 1989 - v1
Checksum:i421F635ECC776996
GO
Isoform Short (identifier: P09919-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     66-68: Missing.

Show »
Length:204
Mass (Da):21,978
Checksum:i5F07C52F13D6A6D3
GO
Isoform 3 (identifier: P09919-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     69-104: Missing.

Note: No experimental confirmation available.
Show »
Length:171
Mass (Da):18,434
Checksum:iC62B366732AD7664
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30A → M AA sequence (PubMed:8554326).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_013073157L → M.1 PublicationCorresponds to variant rs2227329dbSNPEnsembl.1
Natural variantiVAR_013074174A → T.1 PublicationCorresponds to variant rs2227330dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00267366 – 68Missing in isoform Short. 3 Publications3
Alternative sequenceiVSP_04524669 – 104Missing in isoform 3. 1 PublicationAdd BLAST36

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03438 mRNA. Translation: CAA27168.1.
X03656 Genomic DNA. Translation: CAA27291.1.
X03655 mRNA. Translation: CAA27290.1.
M17706 mRNA. Translation: AAA35882.1.
AF388025 Genomic DNA. Translation: AAK62469.1.
CD013926 mRNA. No translation available.
AC090844 Genomic DNA. No translation available.
M13008 mRNA. Translation: AAA03056.1.
CCDSiCCDS11357.1. [P09919-1]
CCDS11358.2. [P09919-3]
CCDS42314.1. [P09919-2]
PIRiA24573.
A25093. FQHUGL.
RefSeqiNP_000750.1. NM_000759.3. [P09919-1]
NP_001171618.1. NM_001178147.1.
NP_757373.1. NM_172219.2. [P09919-2]
NP_757374.2. NM_172220.2. [P09919-3]
UniGeneiHs.2233.

Genome annotation databases

EnsembliENST00000225474; ENSP00000225474; ENSG00000108342. [P09919-1]
ENST00000394148; ENSP00000377704; ENSG00000108342. [P09919-3]
ENST00000394149; ENSP00000377705; ENSG00000108342. [P09919-2]
GeneIDi1440.
KEGGihsa:1440.
UCSCiuc002htp.4. human. [P09919-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Neupogen/Granulokine

Clinical information on Neupogen/Granulokine

SeattleSNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03438 mRNA. Translation: CAA27168.1.
X03656 Genomic DNA. Translation: CAA27291.1.
X03655 mRNA. Translation: CAA27290.1.
M17706 mRNA. Translation: AAA35882.1.
AF388025 Genomic DNA. Translation: AAK62469.1.
CD013926 mRNA. No translation available.
AC090844 Genomic DNA. No translation available.
M13008 mRNA. Translation: AAA03056.1.
CCDSiCCDS11357.1. [P09919-1]
CCDS11358.2. [P09919-3]
CCDS42314.1. [P09919-2]
PIRiA24573.
A25093. FQHUGL.
RefSeqiNP_000750.1. NM_000759.3. [P09919-1]
NP_001171618.1. NM_001178147.1.
NP_757373.1. NM_172219.2. [P09919-2]
NP_757374.2. NM_172220.2. [P09919-3]
UniGeneiHs.2233.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CD9X-ray2.80A/C31-207[»]
1GNCNMR-A31-207[»]
1PGRX-ray3.50A/C/E/G31-207[»]
1RHGX-ray2.20A/B/C31-207[»]
2D9QX-ray2.80A31-207[»]
ProteinModelPortaliP09919.
SMRiP09919.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61120N.
STRINGi9606.ENSP00000225474.

Protein family/group databases

Allergomei8367. Hom s G-CSF.

PTM databases

iPTMnetiP09919.
PhosphoSitePlusiP09919.

Polymorphism and mutation databases

BioMutaiCSF3.
DMDMi117564.

Proteomic databases

EPDiP09919.
PaxDbiP09919.
PeptideAtlasiP09919.
PRIDEiP09919.

Protocols and materials databases

DNASUi1440.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000225474; ENSP00000225474; ENSG00000108342. [P09919-1]
ENST00000394148; ENSP00000377704; ENSG00000108342. [P09919-3]
ENST00000394149; ENSP00000377705; ENSG00000108342. [P09919-2]
GeneIDi1440.
KEGGihsa:1440.
UCSCiuc002htp.4. human. [P09919-1]

Organism-specific databases

CTDi1440.
DisGeNETi1440.
GeneCardsiCSF3.
HGNCiHGNC:2438. CSF3.
HPAiCAB016131.
HPA001412.
MIMi138970. gene.
neXtProtiNX_P09919.
OpenTargetsiENSG00000108342.
PharmGKBiPA26941.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IIM2. Eukaryota.
ENOG4111DA4. LUCA.
GeneTreeiENSGT00390000017328.
HOGENOMiHOG000015371.
HOVERGENiHBG005411.
InParanoidiP09919.
KOiK05423.
OMAiLWHSALW.
OrthoDBiEOG091G0NDU.
PhylomeDBiP09919.
TreeFamiTF337698.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000108342-MONOMER.
ReactomeiR-HSA-449147. Signaling by Interleukins.
SignaLinkiP09919.
SIGNORiP09919.

Miscellaneous databases

EvolutionaryTraceiP09919.
GeneWikiiGranulocyte_colony-stimulating_factor.
GenomeRNAii1440.
PROiP09919.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000108342.
CleanExiHS_CSF3.
ExpressionAtlasiP09919. baseline and differential.
GenevisibleiP09919. HS.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR030474. IL-6/GCSF/MGF.
IPR030473. IL6/GCSF/MGF_CS.
[Graphical view]
PfamiPF16647. GCSF. 1 hit.
[Graphical view]
PIRSFiPIRSF001935. IL6_MGF_GCSF. 1 hit.
PRINTSiPR00433. IL6GCSFMGF.
SMARTiSM00126. IL6. 1 hit.
[Graphical view]
SUPFAMiSSF47266. SSF47266. 1 hit.
PROSITEiPS00254. INTERLEUKIN_6. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCSF3_HUMAN
AccessioniPrimary (citable) accession number: P09919
Secondary accession number(s): A8MXR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 2, 2016
This is version 181 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

PubMed:2420009 misquotes the gene name as "CSF1".Curated

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Pharmaceutical, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.