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P09919 (CSF3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 162. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Granulocyte colony-stimulating factor

Short name=G-CSF
Alternative name(s):
Pluripoietin
INN=Filgrastim
INN=Lenograstim
Gene names
Name:CSF3
Synonyms:C17orf33, GCSF
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length207 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

Subunit structure

Monomer.

Subcellular location

Secreted.

Post-translational modification

O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).

Pharmaceutical use

Available under the names Neupogen or Granulokine (Amgen/Roche) and Granocyte (Rhone-Poulenc). Used to treat neutropenia (a disorder characterized by an extremely low number of neutrophils in blood).

Sequence similarities

Belongs to the IL-6 superfamily.

Caution

Ref.7 misquotes the gene name as "CSF1".

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainSignal
   Molecular functionCytokine
Growth factor
   PTMDisulfide bond
Glycoprotein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Pharmaceutical
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to cytokine stimulus

Inferred from direct assay PubMed 23001182. Source: BHF-UCL

cellular response to lipopolysaccharide

Inferred from electronic annotation. Source: Ensembl

cytokine-mediated signaling pathway

Non-traceable author statement PubMed 15664994. Source: UniProtKB

granulocyte differentiation

Non-traceable author statement PubMed 15664994. Source: UniProtKB

immune response

Inferred from electronic annotation. Source: InterPro

multicellular organismal development

Traceable author statement Ref.7. Source: ProtInc

negative regulation of neuron death

Inferred from electronic annotation. Source: Ensembl

positive regulation of actin cytoskeleton reorganization

Inferred from direct assay PubMed 23001182. Source: BHF-UCL

positive regulation of actin filament polymerization

Inferred from direct assay PubMed 23001182. Source: BHF-UCL

positive regulation of cell proliferation

Traceable author statement Ref.7. Source: ProtInc

positive regulation of peptidyl-serine phosphorylation

Inferred from direct assay PubMed 23001182. Source: BHF-UCL

positive regulation of peptidyl-tyrosine phosphorylation

Inferred from direct assay PubMed 23001182. Source: BHF-UCL

positive regulation of phosphatidylinositol 3-kinase signaling

Inferred from direct assay PubMed 23001182. Source: BHF-UCL

positive regulation of protein binding

Inferred from direct assay PubMed 23001182. Source: BHF-UCL

positive regulation of protein kinase B signaling

Inferred from direct assay PubMed 23001182. Source: BHF-UCL

positive regulation of transcription factor import into nucleus

Inferred from direct assay PubMed 23001182. Source: BHF-UCL

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 23001182. Source: BHF-UCL

   Cellular_componentextracellular space

Traceable author statement Ref.7. Source: ProtInc

   Molecular_functioncytokine activity

Non-traceable author statement PubMed 15664994. Source: UniProtKB

enzyme binding

Inferred from physical interaction PubMed 12393522. Source: UniProtKB

granulocyte colony-stimulating factor receptor binding

Traceable author statement Ref.7. Source: ProtInc

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: P09919-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: P09919-2)

The sequence of this isoform differs from the canonical sequence as follows:
     66-68: Missing.
Isoform 3 (identifier: P09919-3)

The sequence of this isoform differs from the canonical sequence as follows:
     69-104: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Probable
Chain30 – 207178Granulocyte colony-stimulating factor
PRO_0000015570

Amino acid modifications

Glycosylation1661O-linked (GalNAc...) By similarity
Disulfide bond69 ↔ 75
Disulfide bond97 ↔ 107

Natural variations

Alternative sequence66 – 683Missing in isoform Short.
VSP_002673
Alternative sequence69 – 10436Missing in isoform 3.
VSP_045246
Natural variant1571L → M. Ref.4
Corresponds to variant rs2227329 [ dbSNP | Ensembl ].
VAR_013073
Natural variant1741A → T. Ref.4
Corresponds to variant rs2227330 [ dbSNP | Ensembl ].
VAR_013074

Experimental info

Sequence conflict301A → M AA sequence Ref.8

Secondary structure

...................... 207
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 421F635ECC776996

FASTA20722,293
        10         20         30         40         50         60 
MAGPATQSPM KLMALQLLLW HSALWTVQEA TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA 

        70         80         90        100        110        120 
LQEKLVSECA TYKLCHPEEL VLLGHSLGIP WAPLSSCPSQ ALQLAGCLSQ LHSGLFLYQG 

       130        140        150        160        170        180 
LLQALEGISP ELGPTLDTLQ LDVADFATTI WQQMEELGMA PALQPTQGAM PAFASAFQRR 

       190        200 
AGGVLVASHL QSFLEVSYRV LRHLAQP 

« Hide

Isoform Short [UniParc].

Checksum: 5F07C52F13D6A6D3
Show »

FASTA20421,978
Isoform 3 [UniParc].

Checksum: C62B366732AD7664
Show »

FASTA17118,434

References

« Hide 'large scale' references
[1]"Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor."
Nagata S., Tsuchiya M., Asano S., Kaziro Y., Yamazaki T., Yamamoto O., Hirata Y., Kubota N., Oheda M., Nomura H., Ono M.
Nature 319:415-418(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
[2]"The chromosomal gene structure and two mRNAs for human granulocyte colony-stimulating factor."
Nagata S., Tsuchiya M., Asano S., Yamamoto O., Hirata Y., Kubota N., Oheda M., Nomura H., Yamazaki T.
EMBO J. 5:575-581(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM SHORT), ALTERNATIVE SPLICING.
[3]"Expression of granulocyte colony-stimulating factor by human cell lines."
Devlin J.J., Devlin P.E., Myambo K., Lilly M.B., Rado T.A., Warren M.K.
J. Leukoc. Biol. 41:302-306(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
[4]SeattleSNPs variation discovery resource
Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS MET-157 AND THR-174.
[5]"PCR isolation and cloning of novel splice variant mRNAs from known drug target genes."
Jin P., Fu G.K., Wilson A.D., Yang J., Chien D., Hawkins P.R., Au-Young J., Stuve L.L.
Genomics 83:566-571(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[6]"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. expand/collapse author list , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"Recombinant human granulocyte colony-stimulating factor: effects on normal and leukemic myeloid cells."
Souza L.M., Boone T.C., Gabrilove J., Lai P.H., Zsebo K.M., Murdock D.C., Chazin V.R., Bruszewski J., Lu H., Chen K.K., Barendt J., Platzer E., Moore M.A.S., Mertelsmann R., Welte K.
Science 232:61-66(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 19-207 (ISOFORM SHORT).
[8]"Extracellular domain of granulocyte-colony stimulating factor receptor. Interaction with its ligand and identification of a domain in close proximity of ligand-binding region."
Haniu M., Horan T., Arakawa T., Le J., Katta V., Rohde M.F.
Arch. Biochem. Biophys. 324:344-356(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 30-46.
[9]"Glycosidase digestion, electrophoresis and chromatographic analysis of recombinant human granulocyte colony-stimulating factor glycoforms produced in Chinese hamster ovary cells."
Clogston C.L., Hu S., Boone T.C., Lu H.S.
J. Chromatogr. A 637:55-62(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION AT THR-166.
[10]"Secondary structure of human granulocyte colony-stimulating factor derived from NMR spectroscopy."
Zink T., Ross A., Ambrosius D., Rudolph R., Holak T.A.
FEBS Lett. 314:435-439(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.
[11]"Structure and dynamics of the human granulocyte colony-stimulating factor determined by NMR spectroscopy. Loop mobility in a four-helix-bundle protein."
Zink T., Ross A., Luers K., Cieslar C., Rudolph R., Holak T.A.
Biochemistry 33:8453-8463(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.
[12]"The structure of granulocyte-colony-stimulating factor and its relationship to other growth factors."
Hill C.P., Osslund T.D., Eisenberg D.
Proc. Natl. Acad. Sci. U.S.A. 90:5167-5171(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
[13]"Atomic structure of the GCSF-receptor complex showing a new cytokine-receptor recognition scheme."
Aritomi M., Kunishima N., Okamoto T., Kuroki R., Ota Y., Morikawa K.
Nature 401:713-717(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 31-207 IN COMPLEX WITH CSF3R.
[14]"Homodimeric cross-over structure of the human granulocyte colony-stimulating factor (GCSF) receptor signaling complex."
Tamada T., Honjo E., Maeda Y., Okamoto T., Ishibashi M., Tokunaga M., Kuroki R.
Proc. Natl. Acad. Sci. U.S.A. 103:3135-3140(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 31-207 IN COMPLEX WITH CSF3R.
+Additional computationally mapped references.

Web resources

Neupogen/Granulokine

Clinical information on Neupogen/Granulokine

SeattleSNPs

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X03438 mRNA. Translation: CAA27168.1.
X03656 Genomic DNA. Translation: CAA27291.1.
X03655 mRNA. Translation: CAA27290.1.
M17706 mRNA. Translation: AAA35882.1.
AF388025 Genomic DNA. Translation: AAK62469.1.
CD013926 mRNA. No translation available.
AC090844 Genomic DNA. No translation available.
M13008 mRNA. Translation: AAA03056.1.
CCDSCCDS11357.1. [P09919-1]
CCDS11358.2. [P09919-3]
CCDS42314.1. [P09919-2]
PIRA24573.
FQHUGL. A25093.
RefSeqNP_000750.1. NM_000759.3. [P09919-1]
NP_001171618.1. NM_001178147.1.
NP_757373.1. NM_172219.2. [P09919-2]
NP_757374.2. NM_172220.2. [P09919-3]
UniGeneHs.2233.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1CD9X-ray2.80A/C31-207[»]
1GNCNMR-A31-207[»]
1PGRX-ray3.50A/C/E/G31-207[»]
1RHGX-ray2.20A/B/C31-207[»]
2D9QX-ray2.80A31-207[»]
ProteinModelPortalP09919.
SMRP09919. Positions 34-207.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000225474.

Chemistry

DrugBankDB00099. Filgrastim.
DB00019. Pegfilgrastim.

Protein family/group databases

Allergome8367. Hom s G-CSF.

PTM databases

PhosphoSiteP09919.

Polymorphism databases

DMDM117564.

Proteomic databases

PaxDbP09919.
PRIDEP09919.

Protocols and materials databases

DNASU1440.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000225474; ENSP00000225474; ENSG00000108342. [P09919-1]
ENST00000394148; ENSP00000377704; ENSG00000108342. [P09919-3]
ENST00000394149; ENSP00000377705; ENSG00000108342. [P09919-2]
GeneID1440.
KEGGhsa:1440.
UCSCuc002htp.3. human. [P09919-1]

Organism-specific databases

CTD1440.
GeneCardsGC17P038171.
HGNCHGNC:2438. CSF3.
HPACAB016131.
HPA001412.
MIM138970. gene.
neXtProtNX_P09919.
PharmGKBPA26941.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG47652.
HOGENOMHOG000015371.
HOVERGENHBG005411.
InParanoidP09919.
KOK05423.
OMAKLCHPEE.
PhylomeDBP09919.
TreeFamTF337698.

Enzyme and pathway databases

SignaLinkP09919.

Gene expression databases

ArrayExpressP09919.
BgeeP09919.
CleanExHS_CSF3.
GenevestigatorP09919.

Family and domain databases

Gene3D1.20.1250.10. 1 hit.
InterProIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR003573. IL-6/IL-23/GCSF/MGF.
[Graphical view]
PfamPF00489. IL6. 1 hit.
[Graphical view]
PIRSFPIRSF001935. IL6_MGF_GCSF. 1 hit.
PRINTSPR00433. IL6GCSFMGF.
SMARTSM00126. IL6. 1 hit.
[Graphical view]
SUPFAMSSF47266. SSF47266. 1 hit.
PROSITEPS00254. INTERLEUKIN_6. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP09919.
GeneWikiGranulocyte_colony-stimulating_factor.
GenomeRNAi1440.
NextBio5893.
PROP09919.
SOURCESearch...

Entry information

Entry nameCSF3_HUMAN
AccessionPrimary (citable) accession number: P09919
Secondary accession number(s): A8MXR7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: July 9, 2014
This is version 162 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM