Granulocyte colony-stimulating factor precursor

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Web resources

Cross-references

Entry information

Molecule processing

Amino acid modifications

Natural variations

Experimental info

Secondary structure

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3D structure databases

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Reviewed, UniProtKB/Swiss-Prot P09919 (CSF3_HUMAN)

Last modified July 7, 2009. Version 113. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Protein names

Recommended name:
    Granulocyte colony-stimulating factor
      Short name=G-CSF
Alternative name(s):
    Pluripoietin
    INN=Filgrastim
    INN=Lenograstim

Gene names

Name:	CSF3
Synonyms:	GCSF

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Sequence length	207 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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Function	Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Subunit structure	Monomer.
Subcellular location	Secreted.
Post-translational modification	O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
Pharmaceutical use	Available under the names Neupogen or Granulokine (Amgen/Roche) and Granocyte (Rhone-Poulenc). Used to treat neutropenia (a disorder characterized by an extremely low number of neutrophils in blood).
Sequence similarities	Belongs to the IL-6 superfamily.
Caution	Ref.5 misquotes the gene name as "CSF1".

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Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
Molecular function	Cytokine Growth factor
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Pharmaceutical
Gene Ontology (GO)
Biological process	cytokine-mediated signaling pathway Non-traceable author statement. Source: UniProtKB granulocyte differentiation Non-traceable author statement. Source: UniProtKB immune response Inferred from electronic annotation. Source: InterPro positive regulation of cell proliferation Ref.5 Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Inferred from direct assay. Source: HPA cytoskeleton Inferred from direct assay. Source: HPA extracellular space Ref.5 Traceable author statement. Source: ProtInc
Molecular function	cytokine activity Non-traceable author statement. Source: UniProtKB enzyme binding Inferred from physical interaction. Source: UniProtKB granulocyte colony-stimulating factor receptor binding Ref.5 Traceable author statement. Source: ProtInc
Complete GO annotation...

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Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Signal peptide

1 – 29

Probable

Chain

30 – 207

178

Granulocyte colony-stimulating factor

PRO_0000015570

Glycosylation

166

O-linked (GalNAc...) By similarity

Disulfide bond

69 ↔ 75

Disulfide bond

97 ↔ 107

Alternative sequence

66 – 68

Missing in isoform Short.

VSP_002673

Natural variant

157

L → M: dbSNP rs2227329. Ref.4

VAR_013073

Natural variant

174

A → T: dbSNP rs2227330. Ref.4

VAR_013074

Sequence conflict

A → M AA sequence Ref.6

207

Helix Strand Turn

Details...

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Sequence		Length	Mass (Da)
Isoform Long [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 421F635ECC776996 Show »	FASTA	207	22,293
10 20 30 40 50 60 MAGPATQSPM KLMALQLLLW HSALWTVQEA TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA 70 80 90 100 110 120 LQEKLVSECA TYKLCHPEEL VLLGHSLGIP WAPLSSCPSQ ALQLAGCLSQ LHSGLFLYQG 130 140 150 160 170 180 LLQALEGISP ELGPTLDTLQ LDVADFATTI WQQMEELGMA PALQPTQGAM PAFASAFQRR 190 200 AGGVLVASHL QSFLEVSYRV LRHLAQP « Hide
Isoform Short. Checksum: 5F07C52F13D6A6D3 Show »	FASTA	204	21,978
10 20 30 40 50 60 MAGPATQSPM KLMALQLLLW HSALWTVQEA TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA 70 80 90 100 110 120 LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ 130 140 150 160 170 180 ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG 190 200 VLVASHLQSF LEVSYRVLRH LAQP « Hide

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[1]	"Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor." Nagata S., Tsuchiya M., Asano S., Kaziro Y., Yamazaki T., Yamamoto O., Hirata Y., Kubota N., Oheda M., Nomura H., Ono M. Nature 319:415-418(1986) [PubMed: 3484805] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The chromosomal gene structure and two mRNAs for human granulocyte colony-stimulating factor." Nagata S., Tsuchiya M., Asano S., Yamamoto O., Hirata Y., Kubota N., Oheda M., Nomura H., Yamazaki T. EMBO J. 5:575-581(1986) [PubMed: 2423327] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Expression of granulocyte colony-stimulating factor by human cell lines." Devlin J.J., Devlin P.E., Myambo K., Lilly M.B., Rado T.A., Warren M.K. J. Leukoc. Biol. 41:302-306(1987) [PubMed: 3494801] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	SeattleSNPs variation discovery resource Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS MET-157 AND THR-174.
[5]	"Recombinant human granulocyte colony-stimulating factor: effects on normal and leukemic myeloid cells." Souza L.M., Boone T.C., Gabrilove J., Lai P.H., Zsebo K.M., Murdock D.C., Chazin V.R., Bruszewski J., Lu H., Chen K.K., Barendt J., Platzer E., Moore M.A.S., Mertelsmann R., Welte K. Science 232:61-66(1986) [PubMed: 2420009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 19-207.
[6]	"Extracellular domain of granulocyte-colony stimulating factor receptor. Interaction with its ligand and identification of a domain in close proximity of ligand-binding region." Haniu M., Horan T., Arakawa T., Le J., Katta V., Rohde M.F. Arch. Biochem. Biophys. 324:344-356(1995) [PubMed: 8554326] [Abstract] Cited for: PROTEIN SEQUENCE OF 30-46.
[7]	"Glycosidase digestion, electrophoresis and chromatographic analysis of recombinant human granulocyte colony-stimulating factor glycoforms produced in Chinese hamster ovary cells." Clogston C.L., Hu S., Boone T.C., Lu H.S. J. Chromatogr. A 637:55-62(1993) [PubMed: 7685769] [Abstract] Cited for: GLYCOSYLATION AT THR-166.
[8]	"Secondary structure of human granulocyte colony-stimulating factor derived from NMR spectroscopy." Zink T., Ross A., Ambrosius D., Rudolph R., Holak T.A. FEBS Lett. 314:435-439(1992) [PubMed: 1281794] [Abstract] Cited for: STRUCTURE BY NMR.
[9]	"Structure and dynamics of the human granulocyte colony-stimulating factor determined by NMR spectroscopy. Loop mobility in a four-helix-bundle protein." Zink T., Ross A., Luers K., Cieslar C., Rudolph R., Holak T.A. Biochemistry 33:8453-8463(1994) [PubMed: 7518249] [Abstract] Cited for: STRUCTURE BY NMR.
[10]	"The structure of granulocyte-colony-stimulating factor and its relationship to other growth factors." Hill C.P., Osslund T.D., Eisenberg D. Proc. Natl. Acad. Sci. U.S.A. 90:5167-5171(1993) [PubMed: 7685117] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
[11]	"Atomic structure of the GCSF-receptor complex showing a new cytokine-receptor recognition scheme." Aritomi M., Kunishima N., Okamoto T., Kuroki R., Ota Y., Morikawa K. Nature 401:713-717(1999) [PubMed: 10537111] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 31-207 IN COMPLEX WITH CSF3R.
[12]	"Homodimeric cross-over structure of the human granulocyte colony-stimulating factor (GCSF) receptor signaling complex." Tamada T., Honjo E., Maeda Y., Okamoto T., Ishibashi M., Tokunaga M., Kuroki R. Proc. Natl. Acad. Sci. U.S.A. 103:3135-3140(2006) [PubMed: 16492764] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 31-207 IN COMPLEX WITH CSF3R.
+	Additional computationally mapped references.

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Neupogen/Granulokine

Clinical information on Neupogen/Granulokine

SeattleSNPs

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X03438 mRNA. Translation: CAA27168.1.
X03656 Genomic DNA. Translation: CAA27291.1.
X03655 mRNA. Translation: CAA27290.1.
M17706 mRNA. Translation: AAA35882.1.
AF388025 Genomic DNA. Translation: AAK62469.1.
M13008 Unassigned DNA. Translation: AAA03056.1.

IPI

IPI00018306.
IPI00172503.

PIR

A24573.
FQHUGL. A25093.

RefSeq

NP_000750.1.
NP_757373.1.

UniGene

Hs.2233

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CD9	X-ray	2.80	A/C	31-207	[»]
1GNC	NMR	-	A	31-207	[»]
1PGR	X-ray	3.50	A/C/E/G	31-207	[»]
1RHG	X-ray	2.20	A/B/C	31-207	[»]
2D9Q	X-ray	2.80	A	31-207	[»]

ModBase

Search...

PRIDE

P09919.

Ensembl

ENSG00000108342. Homo sapiens. [Contig view]

GeneID

1440.

KEGG

hsa:1440.

UCSC

uc002htp.1. human.

GeneCards

GC17P035425.

H-InvDB

HIX0013789.

HGNC

HGNC:2438. CSF3.

HPA

CAB016131.
HPA001412.

MIM

138970. gene.

PharmGKB

PA26941.

GenAtlas

Search...

HOGENOM

P09919.

HOVERGEN

P09919.

OMA

P09919. EQVRKIQ.

Bgee

P09919.

CleanEx

HS_CSF3.

GermOnline

ENSG00000108342. Homo sapiens.

InterPro

IPR012351. 4_helix_cytokine_core.
IPR003629. GCSF_MGF.
IPR003573. IL6_MGF_GCSF.
[Graphical view]

Gene3D

G3DSA:1.20.1250.10. 4_helix_cytokine_core. 1 hit.

PANTHER

PTHR10511:SF2. GCSF_MGF. 1 hit.

Pfam

PF00489. IL6. 1 hit.
[Graphical view]

PIRSF

PIRSF001935. IL6_MGF_GCSF. 1 hit.

PRINTS

PR00433. IL6GCSFMGF.

SMART

SM00126. IL6. 1 hit.
[Graphical view]

PROSITE

PS00254. INTERLEUKIN_6. 1 hit.
[Graphical view]

ProtoNet

Search...

DrugBank

DB00099. Filgrastim.
DB00019. Pegfilgrastim.

NextBio

5893.

SOURCE

Search...

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This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: P09919-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P09919-2)

The sequence of this isoform differs from the canonical sequence as follows:
66-68: Missing.

Entry name

CSF3_HUMAN

Accession

Primary (citable) accession number: P09919

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	July 7, 2009
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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