Reviewed, UniProtKB/Swiss-Prot P09918 (LOX3_PEA)

Names and origin

Protein names

Recommended name:
    Seed lipoxygenase-3
    EC=1.13.11.12

Gene names

Name:

LOX1.3

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	861 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. It catalyzes the hydroperoxidation of lipids, containing a cis,cis-1,4-pentadiene structure.
Catalytic activity	Linoleate + O2 = (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoate.
Cofactor	Binds 1 iron ion per subunit By similarity.
Pathway	Lipid metabolism; oxylipin biosynthesis.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis Oxylipin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW oxylipin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW lipoxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 861	861	Seed lipoxygenase-3		PRO_0000220714
Regions
	Domain	41 – 166	126	PLAT
	Domain	169 – 861	693	Lipoxygenase
Sites
	Metal binding	522	1	Iron; catalytic By similarity
	Metal binding	527	1	Iron; catalytic By similarity
	Metal binding	713	1	Iron; catalytic By similarity
	Metal binding	717	1	Iron; catalytic By similarity
	Metal binding	861	1	Iron; via carboxylate; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P09918-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 442510278225D750	FASTA	861	97,629
	10 20 30 40 50 60 MFSGVTGILN RGHKIKGTVV LMRKNVLDIN SLTTVGGVIG QGFDILGSTV DNLTAFLGRS 70 80 90 100 110 120 VSLQLISATK PDATGKGKLG KATFLEGIIS SLPTLGAGQS AFKIHFEWDD DMGIPGAFYI 130 140 150 160 170 180 KNFMQTEFFL VSLTLDDIPN HGSIYFVCNS WIYNAKHHKI DRIFFANQTY LPSETPAPLV 190 200 210 220 230 240 HYREEELNNL RGDGTGERKE WERIYDYDVY NDLGNPDSGE NHARPVLGGS ETYPYPRRGR 250 260 270 280 290 300 TGRKPTRKDP NSESRSDYVY LPRDEAFGHL KSSDFLTYGL KAVSQNVVPA LESVFFDLNF 310 320 330 340 350 360 TPNEFDSFDE VHGLYEGGIK LPTNILSQIS PLPVLKEIFR TDGENTLKYP PPKVIQVSRS 370 380 390 400 410 420 GWMTDEEFAR EMLAGVNPNV ICCLQEFPPR SKLDSQIYGD HTSKISKEHL EPNLEGLTVE 430 440 450 460 470 480 EAIQNKKLFL LDHHDSIMPY LRRINSTSTK AYATRTILFL NNNQNLKPLA IELSLPHPQG 490 500 510 520 530 540 DEHGAVSYVY QPALEGVESS IWLLAKAYVI VNDSCYHQLV SHWLNTHAVV EPFVIATNRH 550 560 570 580 590 600 LSCLHPIYKL LYPHYRDTMN INSLARLSLV NDGGIIEKTF LWGRYSMEMS SKVYKNWVFT 610 620 630 640 650 660 EQALPADLIK RGMAIEDPSS PCGVKLVVED YPYAVDGLEI WAIIKTWVQD YVSLYYTSDE 670 680 690 700 710 720 KLRQDSELQA WWKELVEVGH GDKKNEPWWP KMQTREDLIE VCSIVIWTAS ALHAAVNFGQ 730 740 750 760 770 780 YSYGGLILNR PTLSRRFMPE KGSAEFEELV KSPQKAYLKT ITPKFQTLID LSVIEILSRH 790 800 810 820 830 840 ASDELYLGER DNPNWTSDKR ALEAFKKFGN KLAEIEKKLT QRNNDEKLRN RHGPVEMPYT 850 860 LLYPSSKEGL TFRGIPNSIS I

References

[1]	"The complete amino acid sequence of a pea (Pisum sativum) seed lipoxygenase predicted from a near full-length cDNA." Ealing P.M., Casey R. Biochem. J. 253:915-918(1988) [PubMed: 3140791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Birte.
[2]	Casey R. Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Birte.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X07807 mRNA. Translation: CAA30666.1. X78581 Genomic DNA. Translation: CAA55319.1.
PIR	S01142.
3D structure databases
HSSP	HSSP built from PDB template 1F8N based on UniProtKB P08170.
SMR	P09918. Positions 39-861.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.13.11.12. 287.
Family and domain databases
InterPro	IPR000907. LipOase. IPR013819. LipOase_C. IPR001024. LipOase_LH2. IPR001246. LipOase_pln. [Graphical view]
Gene3D	G3DSA:2.60.60.20. Lipase_LipOase. 1 hit.
PANTHER	PTHR11771. LipOase. 1 hit.
Pfam	PF00305. Lipoxygenase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PRINTS	PR00087. LIPOXYGENASE. PR00468. PLTLPOXGNASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
PROSITE	PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LOX3_PEA

Accession

Primary (citable) accession number: P09918

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)