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P09915 (MTBR_BPRH1) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase Rho11sI

Short name=M.Rho11sI
EC=2.1.1.37
Alternative name(s):
Bsu P11s
Cytosine-specific methyltransferase Rho11sI
OrganismBacillus phage rho11s (Bacteriophage rho-11s)
Taxonomic identifier10735 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdalikevirusunclassified Lambda-like viruses
Virus hostBacillus subtilis [TaxID: 1423]

Protein attributes

Sequence length503 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This enzyme methylates cytosine within the sequences GGCC and GAGCTC.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 503503Modification methylase Rho11sI
PRO_0000087869

Regions

Domain4 – 500497SAM-dependent MTase C5-type

Sites

Active site781 By similarity

Sequences

Sequence LengthMass (Da)Tools
P09915 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: AAAFB8FE01B8129E

FASTA50357,130
        10         20         30         40         50         60 
MSKLRVMSLF SGIGAFEAAL RNIGVEYELV GFSEIDKYAI KSYCAIHNAD EQLNFGDVSK 

        70         80         90        100        110        120 
IDKKKLPEFD LLVGGSPCQS FSVAGYRKGF EDTRGTLFFQ YIDTLKEKQP RYFVFENVKG 

       130        140        150        160        170        180 
LINHDKGNTL NIMAESFSEV GYRIDLELLN SKFFNVPQNR ERIYIIGVRE DLIENDEWVV 

       190        200        210        220        230        240 
EKGRNDVLSK GKKRLKELNI KSFNFKWSAQ DIVGRRLREI LEEYVDEKYY LSEEKTSKLI 

       250        260        270        280        290        300 
EQIEKPKEKD VVFVGGINVG KRWLNNGKTY SRNFKQGNRV YDSNGIATTL TSQSVGGLGG 

       310        320        330        340        350        360 
QTSLYKVEDP IMIGHIDLKG HDAIKRVYSP DGVSPTLTTM GEGHREPKIA VEYVGNINPS 

       370        380        390        400        410        420 
GKGMNDQVYN SNGLSPTLTT NKGEGVKISV PNPEIRPVLT PEREEKRQNG RRFKEDDEPA 

       430        440        450        460        470        480 
FTVNTIDRHG VAIGEYPKYR IRKLTPLECW RLQAFDEEDF EKALSVGISN SQLYKQAGNS 

       490        500 
ITVTVLESIF KELIHTYVNE ESE 

« Hide

References

[1]"Organization of multispecific DNA methyltransferases encoded by temperate Bacillus subtilis phages."
Behrens B., Noyer-Weidner M., Pawlek B., Lauster R., Balganesh T.S., Trautner T.A.
EMBO J. 6:1137-1142(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Trautner T.A.
Submitted (SEP-1987) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 476.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X05242 Genomic DNA. Translation: CAA28869.1.
PIRCTBPRH. A28137.

3D structure databases

ProteinModelPortalP09915.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE2835. M.Rho11sI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTBR_BPRH1
AccessionPrimary (citable) accession number: P09915
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries