P09915 (MTBR_BPRH1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Modification methylase Rho11sI Short name=M.Rho11sI EC=2.1.1.37 Alternative name(s): Bsu P11s Cytosine-specific methyltransferase Rho11sI
Organism	Bacillus phage rho11s (Bacteriophage rho-11s)
Taxonomic identifier	10735 [NCBI]
Taxonomic lineage	Viruses › dsDNA viruses, no RNA stage › Caudovirales › Siphoviridae › Lambdalikevirus › unclassified Lambda-like viruses
Virus host	Bacillus subtilis [TaxID: 1423]

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme methylates cytosine within the sequences GGCC and GAGCTC.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	C-5 methylation of cytosine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Modification methylase Rho11sI		PRO_0000087869
Sites
	Active site	78	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P09915 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: AAAFB8FE01B8129E	FASTA	503	57,130
	10 20 30 40 50 60 MSKLRVMSLF SGIGAFEAAL RNIGVEYELV GFSEIDKYAI KSYCAIHNAD EQLNFGDVSK 70 80 90 100 110 120 IDKKKLPEFD LLVGGSPCQS FSVAGYRKGF EDTRGTLFFQ YIDTLKEKQP RYFVFENVKG 130 140 150 160 170 180 LINHDKGNTL NIMAESFSEV GYRIDLELLN SKFFNVPQNR ERIYIIGVRE DLIENDEWVV 190 200 210 220 230 240 EKGRNDVLSK GKKRLKELNI KSFNFKWSAQ DIVGRRLREI LEEYVDEKYY LSEEKTSKLI 250 260 270 280 290 300 EQIEKPKEKD VVFVGGINVG KRWLNNGKTY SRNFKQGNRV YDSNGIATTL TSQSVGGLGG 310 320 330 340 350 360 QTSLYKVEDP IMIGHIDLKG HDAIKRVYSP DGVSPTLTTM GEGHREPKIA VEYVGNINPS 370 380 390 400 410 420 GKGMNDQVYN SNGLSPTLTT NKGEGVKISV PNPEIRPVLT PEREEKRQNG RRFKEDDEPA 430 440 450 460 470 480 FTVNTIDRHG VAIGEYPKYR IRKLTPLECW RLQAFDEEDF EKALSVGISN SQLYKQAGNS 490 500 ITVTVLESIF KELIHTYVNE ESE

References

[1]	"Organization of multispecific DNA methyltransferases encoded by temperate Bacillus subtilis phages." Behrens B., Noyer-Weidner M., Pawlek B., Lauster R., Balganesh T.S., Trautner T.A. EMBO J. 6:1137-1142(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Trautner T.A. Submitted (SEP-1987) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 476.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X05242 Genomic DNA. Translation: CAA28869.1.
PIR	CTBPRH. A28137.
3D structure databases
ProteinModelPortal	P09915.
ModBase	Search...
Protein family/group databases
REBASE	2835. M.Rho11sI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR018117. C5_DNA_meth_AS. IPR001525. C5_MeTfrase. [Graphical view]
PANTHER	PTHR10629. PTHR10629. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTBR_BPRH1

Accession

Primary (citable) accession number: P09915

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program