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Protein

Interferon-induced protein with tetratricopeptide repeats 2

Gene

IFIT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

IFN-induced antiviral protein which inhibits expression of viral messenger RNAs lacking 2'-O-methylation of the 5' cap. The ribose 2'-O-methylation would provide a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Binds AU-rich viral RNAs, with or without 5' triphosphorylation, RNA-binding is required for antiviral activity. Can promote apoptosis.1 Publication

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB
  • RNA binding Source: GO_Central

GO - Biological processi

  • apoptotic mitochondrial changes Source: UniProtKB
  • cellular response to interferon-alpha Source: InterPro
  • defense response to virus Source: GO_Central
  • negative regulation of protein binding Source: BHF-UCL
  • positive regulation of apoptotic process Source: UniProtKB
  • response to virus Source: UniProtKB
  • type I interferon signaling pathway Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Antiviral defense, Apoptosis, Immunity, Innate immunity

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000119922-MONOMER.
ReactomeiR-HSA-909733. Interferon alpha/beta signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon-induced protein with tetratricopeptide repeats 2
Short name:
IFIT-2
Alternative name(s):
ISG-54 K
Interferon-induced 54 kDa protein
Short name:
IFI-54K
Short name:
P54
Gene namesi
Name:IFIT2
Synonyms:CIG-42, G10P2, IFI54, ISG54
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:5409. IFIT2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
  • endoplasmic reticulum Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi184R → E: Abolishes RNA-binding. 1 Publication1
Mutagenesisi255K → E: Significantly impairs RNA-binding; when associated with Glu-259. 1 Publication1
Mutagenesisi259R → E: Significantly impairs RNA-binding; when associated with Glu-255. 1 Publication1
Mutagenesisi292R → E: Abolishes RNA-binding. 1 Publication1
Mutagenesisi410K → E: Abolishes RNA-binding. 1 Publication1

Organism-specific databases

DisGeNETi3433.
OpenTargetsiENSG00000119922.
PharmGKBiPA29650.

Polymorphism and mutation databases

BioMutaiIFIT2.
DMDMi124488.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001063472 – 472Interferon-induced protein with tetratricopeptide repeats 2Add BLAST471

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP09913.
PaxDbiP09913.
PeptideAtlasiP09913.
PRIDEiP09913.

PTM databases

iPTMnetiP09913.
PhosphoSitePlusiP09913.

Expressioni

Inductioni

By type I interferons, dsRNAs and viruses.1 Publication

Gene expression databases

BgeeiENSG00000119922.
CleanExiHS_IFIT2.
ExpressionAtlasiP09913. baseline and differential.
GenevisibleiP09913. HS.

Organism-specific databases

HPAiHPA003408.

Interactioni

Subunit structurei

Domain-swapped homodimer. Component of an interferon-dependent multiprotein complex, at least composed of IFIT1, IFIT2 and IFIT3. Interacts with IFIT1 and IFIT3. Interacts with TMEM173/MITA and disrupts its interaction with MAVS or TBK1. Interacts with EIF3E and EIF3C.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
IFIT1P099145EBI-3507167,EBI-745117
IFIT3O148794EBI-3507167,EBI-745127

Protein-protein interaction databases

BioGridi109658. 43 interactors.
DIPiDIP-48848N.
IntActiP09913. 32 interactors.
MINTiMINT-1479068.
STRINGi9606.ENSP00000360891.

Structurei

Secondary structure

1472
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 14Combined sources6
Turni19 – 21Combined sources3
Turni24 – 27Combined sources4
Helixi31 – 41Combined sources11
Helixi52 – 63Combined sources12
Helixi67 – 84Combined sources18
Helixi86 – 88Combined sources3
Turni90 – 93Combined sources4
Helixi94 – 106Combined sources13
Helixi110 – 126Combined sources17
Helixi136 – 149Combined sources14
Helixi153 – 167Combined sources15
Helixi172 – 187Combined sources16
Helixi195 – 204Combined sources10
Helixi209 – 221Combined sources13
Helixi231 – 242Combined sources12
Helixi247 – 259Combined sources13
Helixi263 – 276Combined sources14
Helixi281 – 300Combined sources20
Helixi309 – 329Combined sources21
Turni331 – 333Combined sources3
Helixi337 – 346Combined sources10
Helixi350 – 362Combined sources13
Helixi367 – 383Combined sources17
Helixi388 – 400Combined sources13
Helixi406 – 425Combined sources20
Helixi432 – 445Combined sources14
Helixi448 – 451Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4G1TX-ray2.80A/B1-472[»]
ProteinModelPortaliP09913.
SMRiP09913.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati51 – 89TPR 1PROSITE-ProRule annotation1 PublicationAdd BLAST39
Repeati90 – 135TPR 2PROSITE-ProRule annotation1 PublicationAdd BLAST46
Repeati136 – 171TPR 3PROSITE-ProRule annotation1 PublicationAdd BLAST36
Repeati172 – 208TPR 4PROSITE-ProRule annotation1 PublicationAdd BLAST37
Repeati247 – 280TPR 5PROSITE-ProRule annotation1 PublicationAdd BLAST34
Repeati281 – 335TPR 6PROSITE-ProRule annotation1 PublicationAdd BLAST55
Repeati336 – 366TPR 7PROSITE-ProRule annotation1 PublicationAdd BLAST31
Repeati367 – 405TPR 8PROSITE-ProRule annotation1 PublicationAdd BLAST39
Repeati406 – 448TPR 9PROSITE-ProRule annotation1 PublicationAdd BLAST43

Domaini

The C-terminal part folds into a super-helical structure and has an extensively positively-charged nucleotide-binding channel on its inner surface.

Sequence similaritiesi

Belongs to the IFIT family.Curated
Contains 9 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1124. Eukaryota.
COG0457. LUCA.
GeneTreeiENSGT00390000013876.
HOVERGENiHBG066330.
InParanoidiP09913.
PhylomeDBiP09913.
TreeFamiTF342671.

Family and domain databases

Gene3Di1.25.40.10. 4 hits.
InterProiIPR024124. Interferon-induced_IFIT2.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR10271:SF4. PTHR10271:SF4. 1 hit.
PfamiPF13181. TPR_8. 2 hits.
[Graphical view]
SMARTiSM00028. TPR. 3 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
PROSITEiPS50005. TPR. 1 hit.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09913-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSENNKNSLE SSLRQLKCHF TWNLMEGENS LDDFEDKVFY RTEFQNREFK
60 70 80 90 100
ATMCNLLAYL KHLKGQNEAA LECLRKAEEL IQQEHADQAE IRSLVTWGNY
110 120 130 140 150
AWVYYHMGRL SDVQIYVDKV KHVCEKFSSP YRIESPELDC EEGWTRLKCG
160 170 180 190 200
GNQNERAKVC FEKALEKKPK NPEFTSGLAI ASYRLDNWPP SQNAIDPLRQ
210 220 230 240 250
AIRLNPDNQY LKVLLALKLH KMREEGEEEG EGEKLVEEAL EKAPGVTDVL
260 270 280 290 300
RSAAKFYRRK DEPDKAIELL KKALEYIPNN AYLHCQIGCC YRAKVFQVMN
310 320 330 340 350
LRENGMYGKR KLLELIGHAV AHLKKADEAN DNLFRVCSIL ASLHALADQY
360 370 380 390 400
EDAEYYFQKE FSKELTPVAK QLLHLRYGNF QLYQMKCEDK AIHHFIEGVK
410 420 430 440 450
INQKSREKEK MKDKLQKIAK MRLSKNGADS EALHVLAFLQ ELNEKMQQAD
460 470
EDSERGLESG SLIPSASSWN GE
Length:472
Mass (Da):54,632
Last modified:July 1, 1989 - v1
Checksum:i3CF11319A5008FB9
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05261579E → A.Corresponds to variant rs17468739dbSNPEnsembl.1
Natural variantiVAR_052616121K → R.Corresponds to variant rs2070845dbSNPEnsembl.1
Natural variantiVAR_014490352D → E.Corresponds to variant rs1727dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14660, M14659 Genomic DNA. Translation: AAA59191.1.
AL353751 Genomic DNA. Translation: CAI12238.1.
X07557 Genomic DNA. Translation: CAA30438.1.
CCDSiCCDS41548.1.
PIRiI59087.
RefSeqiNP_001538.4. NM_001547.4.
UniGeneiHs.437609.

Genome annotation databases

EnsembliENST00000371826; ENSP00000360891; ENSG00000119922.
ENST00000638108; ENSP00000490935; ENSG00000119922.
GeneIDi3433.
KEGGihsa:3433.
UCSCiuc009xts.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14660, M14659 Genomic DNA. Translation: AAA59191.1.
AL353751 Genomic DNA. Translation: CAI12238.1.
X07557 Genomic DNA. Translation: CAA30438.1.
CCDSiCCDS41548.1.
PIRiI59087.
RefSeqiNP_001538.4. NM_001547.4.
UniGeneiHs.437609.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4G1TX-ray2.80A/B1-472[»]
ProteinModelPortaliP09913.
SMRiP09913.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109658. 43 interactors.
DIPiDIP-48848N.
IntActiP09913. 32 interactors.
MINTiMINT-1479068.
STRINGi9606.ENSP00000360891.

PTM databases

iPTMnetiP09913.
PhosphoSitePlusiP09913.

Polymorphism and mutation databases

BioMutaiIFIT2.
DMDMi124488.

Proteomic databases

MaxQBiP09913.
PaxDbiP09913.
PeptideAtlasiP09913.
PRIDEiP09913.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371826; ENSP00000360891; ENSG00000119922.
ENST00000638108; ENSP00000490935; ENSG00000119922.
GeneIDi3433.
KEGGihsa:3433.
UCSCiuc009xts.4. human.

Organism-specific databases

CTDi3433.
DisGeNETi3433.
GeneCardsiIFIT2.
H-InvDBHIX0025911.
HGNCiHGNC:5409. IFIT2.
HPAiHPA003408.
MIMi147040. gene.
neXtProtiNX_P09913.
OpenTargetsiENSG00000119922.
PharmGKBiPA29650.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1124. Eukaryota.
COG0457. LUCA.
GeneTreeiENSGT00390000013876.
HOVERGENiHBG066330.
InParanoidiP09913.
PhylomeDBiP09913.
TreeFamiTF342671.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000119922-MONOMER.
ReactomeiR-HSA-909733. Interferon alpha/beta signaling.

Miscellaneous databases

GeneWikiiIFIT2.
GenomeRNAii3433.
PROiP09913.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000119922.
CleanExiHS_IFIT2.
ExpressionAtlasiP09913. baseline and differential.
GenevisibleiP09913. HS.

Family and domain databases

Gene3Di1.25.40.10. 4 hits.
InterProiIPR024124. Interferon-induced_IFIT2.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR10271:SF4. PTHR10271:SF4. 1 hit.
PfamiPF13181. TPR_8. 2 hits.
[Graphical view]
SMARTiSM00028. TPR. 3 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
PROSITEiPS50005. TPR. 1 hit.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIFIT2_HUMAN
AccessioniPrimary (citable) accession number: P09913
Secondary accession number(s): Q5T767
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 2, 2016
This is version 158 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.