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Protein

60S ribosomal protein L5

Gene

Rpl5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs. It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53.By similarity

GO - Molecular functioni

  • 5S rRNA binding Source: RGD
  • mRNA binding Source: RGD
  • structural constituent of ribosome Source: GO_Central

GO - Biological processi

  • cellular response to inorganic substance Source: RGD
  • positive regulation of isoleucine-tRNA ligase activity Source: RGD
  • positive regulation of methionine-tRNA ligase activity Source: RGD
  • positive regulation of phosphatase activity Source: RGD
  • positive regulation of threonine-tRNA ligase activity Source: RGD
  • ribosomal large subunit assembly Source: GO_Central
  • translation Source: GO_Central

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding

Enzyme and pathway databases

ReactomeiR-RNO-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-RNO-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-RNO-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.
R-RNO-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-RNO-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-RNO-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L5
Gene namesi
Name:Rpl5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi619825. Rpl5.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleusnucleolus By similarity

GO - Cellular componenti

  • aminoacyl-tRNA synthetase multienzyme complex Source: RGD
  • cytoplasm Source: UniProtKB
  • cytosolic large ribosomal subunit Source: RGD
  • cytosolic ribosome Source: RGD
  • intracellular ribonucleoprotein complex Source: RGD
  • nucleolus Source: UniProtKB

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001314362 – 29760S ribosomal protein L5Add BLAST296

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycineBy similarity1
Modified residuei5N6-acetyllysineBy similarity1
Modified residuei48N6-acetyllysineBy similarity1
Modified residuei185PhosphoserineBy similarity1
Modified residuei220N6-acetyllysine; alternateBy similarity1
Cross-linki220Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki220Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei232PhosphothreonineBy similarity1
Modified residuei272PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP09895.
PRIDEiP09895.

PTM databases

iPTMnetiP09895.
PhosphoSitePlusiP09895.
SwissPalmiP09895.

Expressioni

Gene expression databases

BgeeiENSRNOG00000023529.
GenevisibleiP09895. RN.

Interactioni

Subunit structurei

Component of the large ribosomal subunit (LSU). Part of a LSU subcomplex, the 5S RNP which is composed of the 5S RNA, RPL5 and RPL11. Interacts with NVL in an ATP-dependent manner.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Ppp1cbP621422EBI-916235,EBI-352326

Protein-protein interaction databases

IntActiP09895. 2 interactors.
MINTiMINT-4563789.
STRINGi10116.ENSRNOP00000036514.

Structurei

3D structure databases

ProteinModelPortaliP09895.
SMRiP09895.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0875. Eukaryota.
COG0256. LUCA.
GeneTreeiENSGT00390000008456.
HOGENOMiHOG000105947.
HOVERGENiHBG009347.
InParanoidiP09895.
KOiK02932.
OMAiVAEYMES.
OrthoDBiEOG091G0GZO.
PhylomeDBiP09895.
TreeFamiTF300044.

Family and domain databases

HAMAPiMF_01337_A. Ribosomal_L18_A. 1 hit.
InterProiView protein in InterPro
IPR005485. Rbsml_L5_euk/L18_arc.
IPR025607. Rbsml_L5e/L18P_C.
PANTHERiPTHR23410. PTHR23410. 1 hit.
PfamiView protein in Pfam
PF14204. Ribosomal_L18_c. 1 hit.
PF17144. Ribosomal_L5e. 1 hit.
PRINTSiPR00058. RIBOSOMALL5.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09895-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFVKVVKNK AYFKRYQVRF RRRREGKTDY YARKRLVIQD KNKYNTPKYR
60 70 80 90 100
MIVRVTNRDI ICQIAYARIE GDMIVCAAYA HELPKYGVKV GLTNYAAAYC
110 120 130 140 150
TGLLLARRLL NRFGMDKIYE GQVEVNGDEY NVESIDGQPG AFTCYLDAGL
160 170 180 190 200
ARTTTGNKVF GALKGAVDGG LSIPHSTKRF PGYDSESKEF NAEVHRKHIM
210 220 230 240 250
GQNVADYMRY LMEEDEDAYK KQFSQYIKNN VTPDMMEEMY KKAHAAIREN
260 270 280 290
PVYEKKPKRE VKKKRWNRPK MSLAQKKDRV AQKKASFLRA QERAAES
Length:297
Mass (Da):34,459
Last modified:January 23, 2007 - v3
Checksum:iABBB47AF1EBE4366
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti236Missing in AAA42074 (PubMed:3624282).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17419 mRNA. Translation: AAA42074.1.
X06148 mRNA. Translation: CAA29506.1.
BC060561 mRNA. Translation: AAH60561.1.
PIRiS00111. R5RTL5.
RefSeqiNP_112361.1. NM_031099.1.
XP_006250647.1. XM_006250585.3.
UniGeneiRn.92980.

Genome annotation databases

EnsembliENSRNOT00000030428; ENSRNOP00000036514; ENSRNOG00000023529.
GeneIDi81763.
KEGGirno:81763.
UCSCiRGD:619825. rat.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRL5_RAT
AccessioniPrimary (citable) accession number: P09895
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: July 5, 2017
This is version 136 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Ribosomal proteins
    Ribosomal proteins families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families