P09892 (SCP_APLCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Small cardioactive peptides Cleaved into the following 2 chains: Small cardioactive peptide B Short name=SCP B Small cardioactive peptide A Short name=SCP A
Organism	Aplysia californica (California sea hare)
Taxonomic identifier	6500 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Mollusca › Gastropoda › Heterobranchia › Euthyneura › Euopisthobranchia › Aplysiomorpha › Aplysioidea › Aplysiidae › Aplysia

Protein attributes

Sequence length	136 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the stimulation of contractile activity in the gut, the increase of the amplitude of the heart beat, and enhancement of the contractile response of the radula closer muscle.
Subcellular location	Secreted.
Tissue specificity	Highly expressed in the buccal ganglion.
Post-translational modification	Contains three disulfide bonds.
Sequence similarities	Belongs to the SCP family.
Mass spectrometry	Molecular mass is 1140.8±0.3 Da from positions 25 - 33. Determined by MALDI. Ref.2 Molecular mass is 1277.0±0.7 Da from positions 36 - 46. Determined by MALDI. Ref.2 Molecular mass is 9323.8±0.8 Da from positions 49 - 135. Determined by MALDI. Ref.3

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Neuropeptide
PTM	Amidation Disulfide bond
Gene Ontology (GO)
Biological_process	neuropeptide signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Peptide	25 – 33	9	Small cardioactive peptide B		PRO_0000001877
	Peptide	36 – 46	11	Small cardioactive peptide A		PRO_0000311917
	Propeptide	49 – 135	87	Carboxy-terminal peptide		PRO_0000311918
Amino acid modifications
	Modified residue	33	1	Methionine amide
	Modified residue	46	1	Methionine amide

Sequences

	Sequence		Length	Mass (Da)
	P09892 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 8988DB25670CC1EE	FASTA	136	14,773
	10 20 30 40 50 60 METSVSRVTV SLTLLVLIIC SADAMNYLAF PRMGRARPGY LAFPRMGRSQ MKTETGTDCC 70 80 90 100 110 120 GLGMKSEFVI GQEGKEELRH GACSSSVACC AGLREIVDQK QDGVFFSMCV PDFVASRSSE 130 ESSSEVLSKL KSLLQK

References

[1]	"The small cardioactive peptides A and B of Aplysia are derived from a common precursor molecule." Mahon A.C., Lloyd P.E., Weiss K.R., Kupfermann I., Scheller R.H. Proc. Natl. Acad. Sci. U.S.A. 82:3925-3929(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Mass spectrometric survey of interganglionically transported peptides in Aplysia." Li L., Moroz T.P., Garden R.W., Floyd P.D., Weiss K.R., Sweedler J.V. Peptides 19:1425-1433(1998) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY.
[3]	"Peptide profiling of cells with multiple gene products: combining immunochemistry and MALDI mass spectrometry with on-plate microextraction." Li L., Romanova E.V., Rubakhin S.S., Alexeeva V., Weiss K.R., Vilim F.S., Sweedler J.V. Anal. Chem. 72:3867-3874(2000) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M11396 mRNA. Translation: AAA27777.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

SCP_APLCA

Accession

Primary (citable) accession number: P09892

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)