Reviewed,
UniProtKB/Swiss-Prot P09884 (DPOLA_HUMAN)
Last modified
June 16, 2009.
Version 96.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: DNA polymerase alpha catalytic subunit EC=2.7.7.7 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 1462 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Polymerase alpha in a complex with DNA primase is a replicative polymerase. |
| Catalytic activity | Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). |
| Subunit structure | Interacts with SV40 Large T antigen; this interaction allows viral DNA replication. |
| Subcellular location | |
| Post-translational modification | A 165 kDa form is probably produced by proteolytic cleavage at Lys-124. |
| Miscellaneous | In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis. |
| Sequence similarities | Belongs to the DNA polymerase type-B family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||
Molecule processing | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1462 | 1462 | DNA polymerase alpha catalytic subunit | PRO_0000046428 | |||||||||
Regions | |||||||||||||
| DNA binding | 650 – 715 | 66 | Potential | ||||||||||
| DNA binding | 1245 – 1376 | 132 | Potential | ||||||||||
| Zinc finger | 1348 – 1374 | 27 | C4-type | ||||||||||
Sites | |||||||||||||
| Site | 124 – 125 | 2 | Cleavage | ||||||||||
Amino acid modifications | |||||||||||||
| Modified residue | 186 | 1 | Phosphoserine Ref.6 | ||||||||||
| Modified residue | 190 | 1 | Phosphoserine Ref.6 | ||||||||||
| Modified residue | 209 | 1 | Phosphoserine Ref.6 | ||||||||||
Natural variations | |||||||||||||
| Natural variant | 740 | 1 | Y → H: dbSNP rs2230927. | VAR_048877 | |||||||||
Experimental info | |||||||||||||
| Sequence conflict | 503 – 506 | 4 | SPQL → KSTA in CAA29920. Ref.1 | ||||||||||
| Sequence conflict | 837 | 1 | A → G in CAA29920. Ref.1 | ||||||||||
| Sequence conflict | 1405 | 1 | L → C AA sequence Ref.4 | ||||||||||
| Sequence conflict | 1426 | 1 | V → C AA sequence Ref.4 | ||||||||||
Secondary structure | |||||||||||||
Helix Strand Turn | |||||||||||||
| Helix | 1346 – 1358 | 13 | |||||||||||
| Beta strand | 1366 – 1369 | 4 | |||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Human DNA polymerase alpha gene expression is cell proliferation dependent and its primary structure is similar to both prokaryotic and eukaryotic replicative DNA polymerases." Wong S.W., Wahl A.F., Yuan P.-M., Arai N., Pearson B.E., Arai K., Korn D., Hunkapiller M.W., Wang T.S.-F. EMBO J. 7:37-47(1988) [PubMed: 3359994] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | NIEHS SNPs program Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "Human DNA polymerase alpha gene: sequences controlling expression in cycling and serum-stimulated cells." Pearson B.E., Nasheuer H.-P., Wang T.S.-F. Mol. Cell. Biol. 11:2081-2095(1991) [PubMed: 2005899] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-8. |
| [4] | "Human DNA polymerase alpha catalytic polypeptide binds ConA and RCA and contains a specific labile site in the N-terminus." Hsi K.-L., Copeland W.C., Wang T.S.-F. Nucleic Acids Res. 18:6231-6237(1990) [PubMed: 2243771] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-37 AND 1405-1426, PROTEOLYTIC PROCESSING AT LYS-124. |
| [5] | "T-antigen-DNA polymerase alpha complex implicated in simian virus 40 DNA replication." Smale S.T., Tjian R. Mol. Cell. Biol. 6:4077-4087(1986) [PubMed: 3025630] [Abstract] Cited for: INTERACTION WITH SV40 LARGE T ANTIGEN. |
| [6] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-186; SER-190 AND SER-209, MASS SPECTROMETRY. |
| [7] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [8] | "Nuclear magnetic resonance structures of the zinc finger domain of human DNA polymerase-alpha." Evanics F., Maurmann L., Yang W.W., Bose R.N. Biochim. Biophys. Acta 1651:163-171(2003) [PubMed: 14499601] [Abstract] Cited for: STRUCTURE BY NMR OF 1345-1382. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| X06745 mRNA. Translation: CAA29920.1. AY275833 Genomic DNA. Translation: AAP13534.1. M64481 Genomic DNA. Translation: AAA52318.1. | |||||||||||||||||||||||||
| IPI | IPI00220317. | ||||||||||||||||||||||||
| PIR | DJHUAC. S00257. | ||||||||||||||||||||||||
| RefSeq | NP_058633.2. | ||||||||||||||||||||||||
| UniGene | Hs.567319 | ||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||
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| ModBase | Search... | ||||||||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||||||||
| IntAct | P09884. 4 interactions. | ||||||||||||||||||||||||
PTM databases | |||||||||||||||||||||||||
| PhosphoSite | P09884. | ||||||||||||||||||||||||
Proteomic databases | |||||||||||||||||||||||||
| PRIDE | P09884. | ||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||
| Ensembl | ENSG00000101868. Homo sapiens. [Contig view] | ||||||||||||||||||||||||
| GeneID | 5422. | ||||||||||||||||||||||||
| KEGG | hsa:5422. | ||||||||||||||||||||||||
Organism-specific databases | |||||||||||||||||||||||||
| GeneCards | GC0XP024622. | ||||||||||||||||||||||||
| H-InvDB | HIX0028476. | ||||||||||||||||||||||||
| HGNC | HGNC:9173. POLA1. | ||||||||||||||||||||||||
| HPA | CAB012274. HPA002947. | ||||||||||||||||||||||||
| MIM | 312040. gene. | ||||||||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||||||||
| HOVERGEN | P09884. | ||||||||||||||||||||||||
Enzyme and pathway databases | |||||||||||||||||||||||||
| BRENDA | 2.7.7.7. 247. | ||||||||||||||||||||||||
| Reactome | REACT_152. Cell Cycle, Mitotic. REACT_383. DNA Replication. REACT_7970. Telomere Maintenance. | ||||||||||||||||||||||||
Gene expression databases | |||||||||||||||||||||||||
| ArrayExpress | P09884. | ||||||||||||||||||||||||
| Bgee | P09884. | ||||||||||||||||||||||||
| CleanEx | HS_POLA1. | ||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||
| InterPro | IPR006172. DNA-dir_DNA_pol_B. IPR006134. DNA-dir_DNA_pol_B_cons-reg. IPR017966. DNA-dir_DNA_pol_B_cons_reg2. IPR017964. DNA-dir_DNA_pol_B_CS. IPR006133. DNA-dir_DNA_pol_B_exonuc. IPR004578. DNA-dir_DNA_pol_B_pol2. IPR015088. Znf_DNA-dir_DNA_pol_B_alpha. [Graphical view] | ||||||||||||||||||||||||
| PANTHER | PTHR10322. DNA_pol_B. 1 hit. | ||||||||||||||||||||||||
| Pfam | PF00136. DNA_pol_B. 1 hit. PF03104. DNA_pol_B_exo. 1 hit. PF08996. zf-DNA_Pol. 1 hit. [Graphical view] | ||||||||||||||||||||||||
| PRINTS | PR00106. DNAPOLB. | ||||||||||||||||||||||||
| SMART | SM00486. POLBc. 1 hit. [Graphical view] | ||||||||||||||||||||||||
| TIGRFAMs | TIGR00592. pol2. 1 hit. | ||||||||||||||||||||||||
| PROSITE | PS00116. DNA_POLYMERASE_B. 1 hit. [Graphical view] | ||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||
Other Resources | |||||||||||||||||||||||||
| DrugBank | DB00631. Clofarabine. DB01073. Fludarabine. | ||||||||||||||||||||||||
| NextBio | 20977. | ||||||||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||||||||
Entry information
| Entry name | DPOLA_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P09884 Secondary accession number(s): Q86UQ7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome X Human chromosome X: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


