Colicin-E9 - Escherichia coli

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P09883 (CEA9_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 88. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Colicin-E9
EC=3.1.-.-

Gene names

Name:	col
Synonyms:	cei

Encoded on

Plasmid ColE9

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	582 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

This plasmid-coded bactericidal protein is an endonuclease active on both single- and double-stranded DNA but with undefined specificity.

Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

Sequence similarities

Belongs to the colicin/pyosin nuclease family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Antibiotic Antimicrobial Bacteriocin Endonuclease Hydrolase Nuclease
Technical term	3D-structure Plasmid
Gene Ontology (GO)
Biological_process	cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	extrachromosomal circular DNA Inferred from electronic annotation. Source: InterPro
Molecular_function	endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 582

582

Colicin-E9

PRO_0000218684

Sites

Metal binding

550

Zinc By similarity

Metal binding

575

Zinc By similarity

Metal binding

579

Zinc By similarity

Experimental info

Sequence conflict

511

K → R Ref.3

Secondary structure

582

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P09883 [UniParc].

Last modified October 1, 1996. Version 4.
Checksum: 47A71B57B45AFDD9
Show »

FASTA

582

61,587

        10         20         30         40         50         60 
MSGGDGRGHN TGAHSTSGNI NGGPTGIGVS GGASDGSGWS SENNPWGGGS GSGIHWGGGS 

        70         80         90        100        110        120 
GRGNGGGNGN SGGGSGTGGN LSAVAAPVAF GFPALSTPGA GGLAVSISAS ELSAAIAGII 

       130        140        150        160        170        180 
AKLKKVNLKF TPFGVVLSSL IPSEIAKDDP NMMSKIVTSL PADDITESPV SSLPLDKATV 

       190        200        210        220        230        240 
NVNVRVVDDV KDERQNISVV SGVPMSVPVV DAKPTERPGV FTASIPGAPV LNISVNDSTP 

       250        260        270        280        290        300 
AVQTLSPGVT NNTDKDVRPA GFTQGGNTRD AVIRFPKDSG HNAVYVSVSD VLSPDQVKQR 

       310        320        330        340        350        360 
QDEENRRQQE WDATHPVEAA ERNYERARAE LNQANEDVAR NQERQAKAVQ VYNSRKSELD 

       370        380        390        400        410        420 
AANKTLADAI AEIKQFNRFA HDPMAGGHRM WQMAGLKAQR AQTDVNNKQA AFDAAAKEKS 

       430        440        450        460        470        480 
DADAALSAAQ ERRKQKENKE KDAKDKLDKE SKRNKPGKAT GKGKPVGDKW LDDAGKDSGA 

       490        500        510        520        530        540 
PIPDRIADKL RDKEFKSFDD FRKAVWEEVS KDPELSKNLN PSNKSSVSKG YSPFTPKNQQ 

       550        560        570        580 
VGGRKVYELH HDKPISQGGE VYDMDNIRVT TPKRHIDIHR GK

« Hide

References

[1]	James R. Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequences from the colicin E5, E6 and E9 operons: presence of a degenerate transposon-like structure in the ColE9-J plasmid." Lau P.C.K., Condie J.A. Mol. Gen. Genet. 217:269-277(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 378-582.
[3]	"Complete nucleotide sequence of the colicin E9 (cei) gene." Eaton T., James R. Nucleic Acids Res. 17:1761-1761(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 509-582.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X12591 Genomic DNA. Translation: CAA31104.1.
X15858 Genomic DNA. Translation: CAA33862.1.

PIR

PQ0032.

RefSeq

YP_002533537.1. NC_011977.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BXI	X-ray	2.05	B	450-582	[»]
1EMV	X-ray	1.70	B	450-582	[»]
1FR2	X-ray	1.60	B	450-582	[»]
1FSJ	X-ray	1.80	B/C/D/E	450-582	[»]
1V13	X-ray	2.00	A/B	450-582	[»]
1V14	X-ray	2.90	A/B/C/D	450-582	[»]
1V15	X-ray	2.40	A/B/C/D	450-582	[»]
2GYK	X-ray	1.60	B/F	450-582	[»]
2GZE	X-ray	1.80	B	450-582	[»]
2GZF	X-ray	1.75	B	450-582	[»]
2GZG	X-ray	1.70	B	450-582	[»]
2GZI	X-ray	1.70	B	450-582	[»]
2GZJ	X-ray	1.60	B/F	450-582	[»]
2IVZ	X-ray	2.00	E/F/G/H	32-47	[»]
2K5X	NMR	-	B	450-582	[»]
2VLN	X-ray	1.60	B	450-582	[»]
2VLO	X-ray	1.80	B	450-582	[»]
2VLP	X-ray	2.00	B	450-582	[»]
2VLQ	X-ray	1.60	B	450-582	[»]
2WPT	X-ray	1.78	B	450-582	[»]
3O0E	X-ray	3.01	L/M/N/O/P/Q	2-18	[»]

DisProt

DP00342.

ProteinModelPortal

P09883.

SMR

P09883. Positions 82-582.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-16992N.

IntAct

P09883. 1 interaction.

Protein family/group databases

TCDB

1.C.1.4.1. channel-forming colicin family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

7377410.

Phylogenomic databases

ProtClustDB

CLSK2479949.

Family and domain databases

Gene3D

1.10.287.620. 1 hit.

InterPro

IPR024575. Cloacin_colicin_fam.
IPR024622. Colicin/pyocin_DNase_dom.
IPR024566. Colicin_R_dom.
IPR003615. HNH_nuc.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view]

Pfam

PF03515. Cloacin. 1 hit.
PF12639. Colicin-DNase. 1 hit.
PF11570. E2R135. 1 hit.
[Graphical view]

PRINTS

PR01295. CLOACIN.

SMART

SM00507. HNHc. 1 hit.
[Graphical view]

SUPFAM

SSF69369. Pyoson/cloacin_translocation. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P09883.

Entry information

Entry name

CEA9_ECOLX

Accession

Primary (citable) accession number: P09883

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 88 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents