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Protein

Colicin-E9

Gene

col

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This plasmid-coded bactericidal protein is an endonuclease active on both single- and double-stranded DNA but with undefined specificity.
Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi550ZincBy similarity1
Metal bindingi575ZincBy similarity1
Metal bindingi579ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Bacteriocin, Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

TCDBi1.C.1.4.1. the channel-forming colicin (colicin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Colicin-E9 (EC:3.1.-.-)
Gene namesi
Name:col
Synonyms:cei
Encoded oniPlasmid ColE90 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002186841 – 582Colicin-E9Add BLAST582

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
tolBP0A8555EBI-1029888,EBI-7180728From a different organism.

Protein-protein interaction databases

DIPiDIP-16992N.
IntActiP09883. 3 interactors.
MINTiMINT-1771504.

Structurei

Secondary structure

1582
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi38 – 40Combined sources3
Beta strandi94 – 96Combined sources3
Beta strandi102 – 104Combined sources3
Helixi114 – 124Combined sources11
Beta strandi133 – 136Combined sources4
Helixi142 – 148Combined sources7
Beta strandi155 – 161Combined sources7
Helixi162 – 164Combined sources3
Beta strandi170 – 173Combined sources4
Beta strandi178 – 191Combined sources14
Beta strandi194 – 211Combined sources18
Beta strandi221 – 224Combined sources4
Beta strandi230 – 233Combined sources4
Turni262 – 267Combined sources6
Beta strandi268 – 274Combined sources7
Helixi277 – 279Combined sources3
Beta strandi284 – 290Combined sources7
Helixi294 – 313Combined sources20
Helixi316 – 375Combined sources60
Helixi376 – 381Combined sources6
Helixi452 – 454Combined sources3
Beta strandi457 – 460Combined sources4
Helixi470 – 473Combined sources4
Beta strandi476 – 478Combined sources3
Helixi484 – 490Combined sources7
Beta strandi494 – 497Combined sources4
Helixi498 – 511Combined sources14
Helixi513 – 516Combined sources4
Beta strandi517 – 519Combined sources3
Helixi521 – 528Combined sources8
Helixi537 – 539Combined sources3
Beta strandi548 – 553Combined sources6
Helixi555 – 557Combined sources3
Beta strandi561 – 563Combined sources3
Helixi564 – 566Combined sources3
Beta strandi567 – 570Combined sources4
Helixi572 – 579Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BXIX-ray2.05B449-582[»]
1EMVX-ray1.70B450-582[»]
1FR2X-ray1.60B450-582[»]
1FSJX-ray1.80B/C/D/E450-582[»]
1V13X-ray2.00A/B450-582[»]
1V14X-ray2.90A/B/C/D450-582[»]
1V15X-ray2.40A/B/C/D450-582[»]
2GYKX-ray1.60B/F450-582[»]
2GZEX-ray1.80B450-582[»]
2GZFX-ray1.75B450-582[»]
2GZGX-ray1.70B450-582[»]
2GZIX-ray1.70B450-582[»]
2GZJX-ray1.60B/F450-582[»]
2IVZX-ray2.00E/F/G/H32-47[»]
2K5XNMR-B450-582[»]
2VLNX-ray1.60B450-582[»]
2VLOX-ray1.80B450-582[»]
2VLPX-ray2.00B450-582[»]
2VLQX-ray1.60B450-582[»]
2WPTX-ray1.78B450-582[»]
3O0EX-ray3.01L/M/N/O/P/Q2-18[»]
4JMLX-ray2.00E32-47[»]
5EW5X-ray3.20A/B/C/D1-582[»]
DisProtiDP00342.
ProteinModelPortaliP09883.
SMRiP09883.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09883.

Family & Domainsi

Sequence similaritiesi

Belongs to the colicin/pyosin nuclease family.Curated

Family and domain databases

Gene3Di1.10.287.620. 1 hit.
3.90.540.10. 1 hit.
InterProiIPR024575. Cloacin_colicin_fam.
IPR024622. Colicin/pyocin_DNase_dom.
IPR024566. Colicin_R_dom.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view]
PfamiPF03515. Cloacin. 1 hit.
PF11570. E2R135. 1 hit.
[Graphical view]
PRINTSiPR01295. CLOACIN.
SUPFAMiSSF69369. SSF69369. 1 hit.

Sequencei

Sequence statusi: Complete.

P09883-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGGDGRGHN TGAHSTSGNI NGGPTGIGVS GGASDGSGWS SENNPWGGGS
60 70 80 90 100
GSGIHWGGGS GRGNGGGNGN SGGGSGTGGN LSAVAAPVAF GFPALSTPGA
110 120 130 140 150
GGLAVSISAS ELSAAIAGII AKLKKVNLKF TPFGVVLSSL IPSEIAKDDP
160 170 180 190 200
NMMSKIVTSL PADDITESPV SSLPLDKATV NVNVRVVDDV KDERQNISVV
210 220 230 240 250
SGVPMSVPVV DAKPTERPGV FTASIPGAPV LNISVNDSTP AVQTLSPGVT
260 270 280 290 300
NNTDKDVRPA GFTQGGNTRD AVIRFPKDSG HNAVYVSVSD VLSPDQVKQR
310 320 330 340 350
QDEENRRQQE WDATHPVEAA ERNYERARAE LNQANEDVAR NQERQAKAVQ
360 370 380 390 400
VYNSRKSELD AANKTLADAI AEIKQFNRFA HDPMAGGHRM WQMAGLKAQR
410 420 430 440 450
AQTDVNNKQA AFDAAAKEKS DADAALSAAQ ERRKQKENKE KDAKDKLDKE
460 470 480 490 500
SKRNKPGKAT GKGKPVGDKW LDDAGKDSGA PIPDRIADKL RDKEFKSFDD
510 520 530 540 550
FRKAVWEEVS KDPELSKNLN PSNKSSVSKG YSPFTPKNQQ VGGRKVYELH
560 570 580
HDKPISQGGE VYDMDNIRVT TPKRHIDIHR GK
Length:582
Mass (Da):61,587
Last modified:October 1, 1996 - v4
Checksum:i47A71B57B45AFDD9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti511K → R (PubMed:2646600).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12591 Genomic DNA. Translation: CAA31104.1.
X15858 Genomic DNA. Translation: CAA33862.1.
PIRiPQ0032.
RefSeqiWP_012644886.1. NC_011977.1.
YP_002533537.1. NC_011977.1.

Genome annotation databases

GeneIDi7377410.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12591 Genomic DNA. Translation: CAA31104.1.
X15858 Genomic DNA. Translation: CAA33862.1.
PIRiPQ0032.
RefSeqiWP_012644886.1. NC_011977.1.
YP_002533537.1. NC_011977.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BXIX-ray2.05B449-582[»]
1EMVX-ray1.70B450-582[»]
1FR2X-ray1.60B450-582[»]
1FSJX-ray1.80B/C/D/E450-582[»]
1V13X-ray2.00A/B450-582[»]
1V14X-ray2.90A/B/C/D450-582[»]
1V15X-ray2.40A/B/C/D450-582[»]
2GYKX-ray1.60B/F450-582[»]
2GZEX-ray1.80B450-582[»]
2GZFX-ray1.75B450-582[»]
2GZGX-ray1.70B450-582[»]
2GZIX-ray1.70B450-582[»]
2GZJX-ray1.60B/F450-582[»]
2IVZX-ray2.00E/F/G/H32-47[»]
2K5XNMR-B450-582[»]
2VLNX-ray1.60B450-582[»]
2VLOX-ray1.80B450-582[»]
2VLPX-ray2.00B450-582[»]
2VLQX-ray1.60B450-582[»]
2WPTX-ray1.78B450-582[»]
3O0EX-ray3.01L/M/N/O/P/Q2-18[»]
4JMLX-ray2.00E32-47[»]
5EW5X-ray3.20A/B/C/D1-582[»]
DisProtiDP00342.
ProteinModelPortaliP09883.
SMRiP09883.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-16992N.
IntActiP09883. 3 interactors.
MINTiMINT-1771504.

Protein family/group databases

TCDBi1.C.1.4.1. the channel-forming colicin (colicin) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi7377410.

Miscellaneous databases

EvolutionaryTraceiP09883.

Family and domain databases

Gene3Di1.10.287.620. 1 hit.
3.90.540.10. 1 hit.
InterProiIPR024575. Cloacin_colicin_fam.
IPR024622. Colicin/pyocin_DNase_dom.
IPR024566. Colicin_R_dom.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view]
PfamiPF03515. Cloacin. 1 hit.
PF11570. E2R135. 1 hit.
[Graphical view]
PRINTSiPR01295. CLOACIN.
SUPFAMiSSF69369. SSF69369. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCEA9_ECOLX
AccessioniPrimary (citable) accession number: P09883
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 106 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.