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P09883

- CEA9_ECOLX

UniProt

P09883 - CEA9_ECOLX

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Protein

Colicin-E9

Gene
col, cei
Organism
Escherichia coli
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

This plasmid-coded bactericidal protein is an endonuclease active on both single- and double-stranded DNA but with undefined specificity.
Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi550 – 5501Zinc By similarity
Metal bindingi575 – 5751Zinc By similarity
Metal bindingi579 – 5791Zinc By similarity

GO - Molecular functioni

  1. endonuclease activity Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. protein binding Source: IntAct

GO - Biological processi

  1. cytolysis Source: UniProtKB-KW
  2. defense response to bacterium Source: UniProtKB-KW
  3. pathogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Bacteriocin, Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

TCDBi1.C.1.4.1. the channel-forming colicin (colicin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Colicin-E9 (EC:3.1.-.-)
Gene namesi
Name:col
Synonyms:cei
Encoded oniPlasmid ColE90 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

GO - Cellular componenti

  1. extrachromosomal circular DNA Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 582582Colicin-E9PRO_0000218684Add
BLAST

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
tolBP0A8555EBI-1029888,EBI-7180728From a different organism.

Protein-protein interaction databases

DIPiDIP-16992N.
IntActiP09883. 3 interactions.
MINTiMINT-1771504.

Structurei

Secondary structure

1
582
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi38 – 403
Helixi452 – 4543
Beta strandi457 – 4604
Helixi470 – 4734
Beta strandi476 – 4783
Helixi484 – 4907
Beta strandi494 – 4974
Helixi498 – 51114
Helixi513 – 5164
Beta strandi517 – 5193
Helixi521 – 5288
Helixi537 – 5393
Beta strandi548 – 5536
Helixi555 – 5573
Beta strandi561 – 5633
Helixi564 – 5663
Beta strandi567 – 5704
Helixi572 – 5798

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BXIX-ray2.05B449-582[»]
1EMVX-ray1.70B450-582[»]
1FR2X-ray1.60B450-582[»]
1FSJX-ray1.80B/C/D/E450-582[»]
1V13X-ray2.00A/B450-582[»]
1V14X-ray2.90A/B/C/D450-582[»]
1V15X-ray2.40A/B/C/D450-582[»]
2GYKX-ray1.60B/F450-582[»]
2GZEX-ray1.80B450-582[»]
2GZFX-ray1.75B450-582[»]
2GZGX-ray1.70B450-582[»]
2GZIX-ray1.70B450-582[»]
2GZJX-ray1.60B/F450-582[»]
2IVZX-ray2.00E/F/G/H32-47[»]
2K5XNMR-B450-582[»]
2VLNX-ray1.60B450-582[»]
2VLOX-ray1.80B450-582[»]
2VLPX-ray2.00B450-582[»]
2VLQX-ray1.60B450-582[»]
2WPTX-ray1.78B450-582[»]
3O0EX-ray3.01L/M/N/O/P/Q2-18[»]
4JMLX-ray2.00E32-47[»]
DisProtiDP00342.
ProteinModelPortaliP09883.
SMRiP09883. Positions 82-582.

Miscellaneous databases

EvolutionaryTraceiP09883.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.287.620. 1 hit.
3.90.540.10. 1 hit.
InterProiIPR024575. Cloacin_colicin_fam.
IPR024622. Colicin/pyocin_DNase_dom.
IPR024566. Colicin_R_dom.
IPR003615. HNH_nuc.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view]
PfamiPF03515. Cloacin. 1 hit.
PF12639. Colicin-DNase. 1 hit.
PF11570. E2R135. 1 hit.
[Graphical view]
PRINTSiPR01295. CLOACIN.
SMARTiSM00507. HNHc. 1 hit.
[Graphical view]
SUPFAMiSSF69369. SSF69369. 1 hit.

Sequencei

Sequence statusi: Complete.

P09883-1 [UniParc]FASTAAdd to Basket

« Hide

MSGGDGRGHN TGAHSTSGNI NGGPTGIGVS GGASDGSGWS SENNPWGGGS    50
GSGIHWGGGS GRGNGGGNGN SGGGSGTGGN LSAVAAPVAF GFPALSTPGA 100
GGLAVSISAS ELSAAIAGII AKLKKVNLKF TPFGVVLSSL IPSEIAKDDP 150
NMMSKIVTSL PADDITESPV SSLPLDKATV NVNVRVVDDV KDERQNISVV 200
SGVPMSVPVV DAKPTERPGV FTASIPGAPV LNISVNDSTP AVQTLSPGVT 250
NNTDKDVRPA GFTQGGNTRD AVIRFPKDSG HNAVYVSVSD VLSPDQVKQR 300
QDEENRRQQE WDATHPVEAA ERNYERARAE LNQANEDVAR NQERQAKAVQ 350
VYNSRKSELD AANKTLADAI AEIKQFNRFA HDPMAGGHRM WQMAGLKAQR 400
AQTDVNNKQA AFDAAAKEKS DADAALSAAQ ERRKQKENKE KDAKDKLDKE 450
SKRNKPGKAT GKGKPVGDKW LDDAGKDSGA PIPDRIADKL RDKEFKSFDD 500
FRKAVWEEVS KDPELSKNLN PSNKSSVSKG YSPFTPKNQQ VGGRKVYELH 550
HDKPISQGGE VYDMDNIRVT TPKRHIDIHR GK 582
Length:582
Mass (Da):61,587
Last modified:October 1, 1996 - v4
Checksum:i47A71B57B45AFDD9
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti511 – 5111K → R1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X12591 Genomic DNA. Translation: CAA31104.1.
X15858 Genomic DNA. Translation: CAA33862.1.
PIRiPQ0032.
RefSeqiWP_012644886.1. NC_011977.1.
YP_002533537.1. NC_011977.1.

Genome annotation databases

GeneIDi7377410.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X12591 Genomic DNA. Translation: CAA31104.1 .
X15858 Genomic DNA. Translation: CAA33862.1 .
PIRi PQ0032.
RefSeqi WP_012644886.1. NC_011977.1.
YP_002533537.1. NC_011977.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1BXI X-ray 2.05 B 449-582 [» ]
1EMV X-ray 1.70 B 450-582 [» ]
1FR2 X-ray 1.60 B 450-582 [» ]
1FSJ X-ray 1.80 B/C/D/E 450-582 [» ]
1V13 X-ray 2.00 A/B 450-582 [» ]
1V14 X-ray 2.90 A/B/C/D 450-582 [» ]
1V15 X-ray 2.40 A/B/C/D 450-582 [» ]
2GYK X-ray 1.60 B/F 450-582 [» ]
2GZE X-ray 1.80 B 450-582 [» ]
2GZF X-ray 1.75 B 450-582 [» ]
2GZG X-ray 1.70 B 450-582 [» ]
2GZI X-ray 1.70 B 450-582 [» ]
2GZJ X-ray 1.60 B/F 450-582 [» ]
2IVZ X-ray 2.00 E/F/G/H 32-47 [» ]
2K5X NMR - B 450-582 [» ]
2VLN X-ray 1.60 B 450-582 [» ]
2VLO X-ray 1.80 B 450-582 [» ]
2VLP X-ray 2.00 B 450-582 [» ]
2VLQ X-ray 1.60 B 450-582 [» ]
2WPT X-ray 1.78 B 450-582 [» ]
3O0E X-ray 3.01 L/M/N/O/P/Q 2-18 [» ]
4JML X-ray 2.00 E 32-47 [» ]
DisProti DP00342.
ProteinModelPortali P09883.
SMRi P09883. Positions 82-582.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-16992N.
IntActi P09883. 3 interactions.
MINTi MINT-1771504.

Protein family/group databases

TCDBi 1.C.1.4.1. the channel-forming colicin (colicin) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 7377410.

Miscellaneous databases

EvolutionaryTracei P09883.

Family and domain databases

Gene3Di 1.10.287.620. 1 hit.
3.90.540.10. 1 hit.
InterProi IPR024575. Cloacin_colicin_fam.
IPR024622. Colicin/pyocin_DNase_dom.
IPR024566. Colicin_R_dom.
IPR003615. HNH_nuc.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view ]
Pfami PF03515. Cloacin. 1 hit.
PF12639. Colicin-DNase. 1 hit.
PF11570. E2R135. 1 hit.
[Graphical view ]
PRINTSi PR01295. CLOACIN.
SMARTi SM00507. HNHc. 1 hit.
[Graphical view ]
SUPFAMi SSF69369. SSF69369. 1 hit.
ProtoNeti Search...

Publicationsi

  1. James R.
    Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequences from the colicin E5, E6 and E9 operons: presence of a degenerate transposon-like structure in the ColE9-J plasmid."
    Lau P.C.K., Condie J.A.
    Mol. Gen. Genet. 217:269-277(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 378-582.
  3. "Complete nucleotide sequence of the colicin E9 (cei) gene."
    Eaton T., James R.
    Nucleic Acids Res. 17:1761-1761(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 509-582.

Entry informationi

Entry nameiCEA9_ECOLX
AccessioniPrimary (citable) accession number: P09883
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 96 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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