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P09883 (CEA9_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Colicin-E9

EC=3.1.-.-
Gene names
Name:col
Synonyms:cei
Encoded onPlasmid ColE9
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length582 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This plasmid-coded bactericidal protein is an endonuclease active on both single- and double-stranded DNA but with undefined specificity.

Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

Sequence similarities

Belongs to the colicin/pyosin nuclease family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

tolBP0A8555EBI-1029888,EBI-7180728From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 582582Colicin-E9
PRO_0000218684

Sites

Metal binding5501Zinc By similarity
Metal binding5751Zinc By similarity
Metal binding5791Zinc By similarity

Experimental info

Sequence conflict5111K → R Ref.3

Secondary structure

................................. 582
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P09883 [UniParc].

Last modified October 1, 1996. Version 4.
Checksum: 47A71B57B45AFDD9

FASTA58261,587
        10         20         30         40         50         60 
MSGGDGRGHN TGAHSTSGNI NGGPTGIGVS GGASDGSGWS SENNPWGGGS GSGIHWGGGS 

        70         80         90        100        110        120 
GRGNGGGNGN SGGGSGTGGN LSAVAAPVAF GFPALSTPGA GGLAVSISAS ELSAAIAGII 

       130        140        150        160        170        180 
AKLKKVNLKF TPFGVVLSSL IPSEIAKDDP NMMSKIVTSL PADDITESPV SSLPLDKATV 

       190        200        210        220        230        240 
NVNVRVVDDV KDERQNISVV SGVPMSVPVV DAKPTERPGV FTASIPGAPV LNISVNDSTP 

       250        260        270        280        290        300 
AVQTLSPGVT NNTDKDVRPA GFTQGGNTRD AVIRFPKDSG HNAVYVSVSD VLSPDQVKQR 

       310        320        330        340        350        360 
QDEENRRQQE WDATHPVEAA ERNYERARAE LNQANEDVAR NQERQAKAVQ VYNSRKSELD 

       370        380        390        400        410        420 
AANKTLADAI AEIKQFNRFA HDPMAGGHRM WQMAGLKAQR AQTDVNNKQA AFDAAAKEKS 

       430        440        450        460        470        480 
DADAALSAAQ ERRKQKENKE KDAKDKLDKE SKRNKPGKAT GKGKPVGDKW LDDAGKDSGA 

       490        500        510        520        530        540 
PIPDRIADKL RDKEFKSFDD FRKAVWEEVS KDPELSKNLN PSNKSSVSKG YSPFTPKNQQ 

       550        560        570        580 
VGGRKVYELH HDKPISQGGE VYDMDNIRVT TPKRHIDIHR GK 

« Hide

References

[1]James R.
Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Nucleotide sequences from the colicin E5, E6 and E9 operons: presence of a degenerate transposon-like structure in the ColE9-J plasmid."
Lau P.C.K., Condie J.A.
Mol. Gen. Genet. 217:269-277(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 378-582.
[3]"Complete nucleotide sequence of the colicin E9 (cei) gene."
Eaton T., James R.
Nucleic Acids Res. 17:1761-1761(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 509-582.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X12591 Genomic DNA. Translation: CAA31104.1.
X15858 Genomic DNA. Translation: CAA33862.1.
PIRPQ0032.
RefSeqYP_002533537.1. NC_011977.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1BXIX-ray2.05B449-582[»]
1EMVX-ray1.70B450-582[»]
1FR2X-ray1.60B450-582[»]
1FSJX-ray1.80B/C/D/E450-582[»]
1V13X-ray2.00A/B450-582[»]
1V14X-ray2.90A/B/C/D450-582[»]
1V15X-ray2.40A/B/C/D450-582[»]
2GYKX-ray1.60B/F450-582[»]
2GZEX-ray1.80B450-582[»]
2GZFX-ray1.75B450-582[»]
2GZGX-ray1.70B450-582[»]
2GZIX-ray1.70B450-582[»]
2GZJX-ray1.60B/F450-582[»]
2IVZX-ray2.00E/F/G/H32-47[»]
2K5XNMR-B450-582[»]
2VLNX-ray1.60B450-582[»]
2VLOX-ray1.80B450-582[»]
2VLPX-ray2.00B450-582[»]
2VLQX-ray1.60B450-582[»]
2WPTX-ray1.78B450-582[»]
3O0EX-ray3.01L/M/N/O/P/Q2-18[»]
4JMLX-ray2.00E32-47[»]
DisProtDP00342.
ProteinModelPortalP09883.
SMRP09883. Positions 82-582.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-16992N.
IntActP09883. 3 interactions.
MINTMINT-1771504.

Protein family/group databases

TCDB1.C.1.4.1. the channel-forming colicin (colicin) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7377410.

Family and domain databases

Gene3D1.10.287.620. 1 hit.
3.90.540.10. 1 hit.
InterProIPR024575. Cloacin_colicin_fam.
IPR024622. Colicin/pyocin_DNase_dom.
IPR024566. Colicin_R_dom.
IPR003615. HNH_nuc.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view]
PfamPF03515. Cloacin. 1 hit.
PF12639. Colicin-DNase. 1 hit.
PF11570. E2R135. 1 hit.
[Graphical view]
PRINTSPR01295. CLOACIN.
SMARTSM00507. HNHc. 1 hit.
[Graphical view]
SUPFAMSSF69369. SSF69369. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP09883.

Entry information

Entry nameCEA9_ECOLX
AccessionPrimary (citable) accession number: P09883
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 95 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references