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Protein

Colicin-E9

Gene

col

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This plasmid-coded bactericidal protein is an endonuclease active on both single- and double-stranded DNA but with undefined specificity.
Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi550 – 5501ZincBy similarity
Metal bindingi575 – 5751ZincBy similarity
Metal bindingi579 – 5791ZincBy similarity

GO - Molecular functioni

  1. endonuclease activity Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cytolysis Source: UniProtKB-KW
  2. defense response to bacterium Source: UniProtKB-KW
  3. pathogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Bacteriocin, Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

TCDBi1.C.1.4.1. the channel-forming colicin (colicin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Colicin-E9 (EC:3.1.-.-)
Gene namesi
Name:col
Synonyms:cei
Encoded oniPlasmid ColE90 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

GO - Cellular componenti

  1. extrachromosomal circular DNA Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 582582Colicin-E9PRO_0000218684Add
BLAST

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
tolBP0A8555EBI-1029888,EBI-7180728From a different organism.

Protein-protein interaction databases

DIPiDIP-16992N.
IntActiP09883. 3 interactions.
MINTiMINT-1771504.

Structurei

Secondary structure

1
582
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi38 – 403Combined sources
Helixi452 – 4543Combined sources
Beta strandi457 – 4604Combined sources
Helixi470 – 4734Combined sources
Beta strandi476 – 4783Combined sources
Helixi484 – 4907Combined sources
Beta strandi494 – 4974Combined sources
Helixi498 – 51114Combined sources
Helixi513 – 5164Combined sources
Beta strandi517 – 5193Combined sources
Helixi521 – 5288Combined sources
Helixi537 – 5393Combined sources
Beta strandi548 – 5536Combined sources
Helixi555 – 5573Combined sources
Beta strandi561 – 5633Combined sources
Helixi564 – 5663Combined sources
Beta strandi567 – 5704Combined sources
Helixi572 – 5798Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BXIX-ray2.05B449-582[»]
1EMVX-ray1.70B450-582[»]
1FR2X-ray1.60B450-582[»]
1FSJX-ray1.80B/C/D/E450-582[»]
1V13X-ray2.00A/B450-582[»]
1V14X-ray2.90A/B/C/D450-582[»]
1V15X-ray2.40A/B/C/D450-582[»]
2GYKX-ray1.60B/F450-582[»]
2GZEX-ray1.80B450-582[»]
2GZFX-ray1.75B450-582[»]
2GZGX-ray1.70B450-582[»]
2GZIX-ray1.70B450-582[»]
2GZJX-ray1.60B/F450-582[»]
2IVZX-ray2.00E/F/G/H32-47[»]
2K5XNMR-B450-582[»]
2VLNX-ray1.60B450-582[»]
2VLOX-ray1.80B450-582[»]
2VLPX-ray2.00B450-582[»]
2VLQX-ray1.60B450-582[»]
2WPTX-ray1.78B450-582[»]
3O0EX-ray3.01L/M/N/O/P/Q2-18[»]
4JMLX-ray2.00E32-47[»]
DisProtiDP00342.
ProteinModelPortaliP09883.
SMRiP09883. Positions 82-582.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09883.

Family & Domainsi

Sequence similaritiesi

Belongs to the colicin/pyosin nuclease family.Curated

Family and domain databases

Gene3Di1.10.287.620. 1 hit.
3.90.540.10. 1 hit.
InterProiIPR024575. Cloacin_colicin_fam.
IPR024622. Colicin/pyocin_DNase_dom.
IPR024566. Colicin_R_dom.
IPR003615. HNH_nuc.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view]
PfamiPF03515. Cloacin. 1 hit.
PF12639. Colicin-DNase. 1 hit.
PF11570. E2R135. 1 hit.
[Graphical view]
PRINTSiPR01295. CLOACIN.
SMARTiSM00507. HNHc. 1 hit.
[Graphical view]
SUPFAMiSSF69369. SSF69369. 1 hit.

Sequencei

Sequence statusi: Complete.

P09883-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGGDGRGHN TGAHSTSGNI NGGPTGIGVS GGASDGSGWS SENNPWGGGS
60 70 80 90 100
GSGIHWGGGS GRGNGGGNGN SGGGSGTGGN LSAVAAPVAF GFPALSTPGA
110 120 130 140 150
GGLAVSISAS ELSAAIAGII AKLKKVNLKF TPFGVVLSSL IPSEIAKDDP
160 170 180 190 200
NMMSKIVTSL PADDITESPV SSLPLDKATV NVNVRVVDDV KDERQNISVV
210 220 230 240 250
SGVPMSVPVV DAKPTERPGV FTASIPGAPV LNISVNDSTP AVQTLSPGVT
260 270 280 290 300
NNTDKDVRPA GFTQGGNTRD AVIRFPKDSG HNAVYVSVSD VLSPDQVKQR
310 320 330 340 350
QDEENRRQQE WDATHPVEAA ERNYERARAE LNQANEDVAR NQERQAKAVQ
360 370 380 390 400
VYNSRKSELD AANKTLADAI AEIKQFNRFA HDPMAGGHRM WQMAGLKAQR
410 420 430 440 450
AQTDVNNKQA AFDAAAKEKS DADAALSAAQ ERRKQKENKE KDAKDKLDKE
460 470 480 490 500
SKRNKPGKAT GKGKPVGDKW LDDAGKDSGA PIPDRIADKL RDKEFKSFDD
510 520 530 540 550
FRKAVWEEVS KDPELSKNLN PSNKSSVSKG YSPFTPKNQQ VGGRKVYELH
560 570 580
HDKPISQGGE VYDMDNIRVT TPKRHIDIHR GK
Length:582
Mass (Da):61,587
Last modified:September 30, 1996 - v4
Checksum:i47A71B57B45AFDD9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti511 – 5111K → R (PubMed:2646600).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12591 Genomic DNA. Translation: CAA31104.1.
X15858 Genomic DNA. Translation: CAA33862.1.
PIRiPQ0032.
RefSeqiWP_012644886.1. NC_011977.1.
YP_002533537.1. NC_011977.1.

Genome annotation databases

GeneIDi7377410.
KEGGipg:7377410.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12591 Genomic DNA. Translation: CAA31104.1.
X15858 Genomic DNA. Translation: CAA33862.1.
PIRiPQ0032.
RefSeqiWP_012644886.1. NC_011977.1.
YP_002533537.1. NC_011977.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BXIX-ray2.05B449-582[»]
1EMVX-ray1.70B450-582[»]
1FR2X-ray1.60B450-582[»]
1FSJX-ray1.80B/C/D/E450-582[»]
1V13X-ray2.00A/B450-582[»]
1V14X-ray2.90A/B/C/D450-582[»]
1V15X-ray2.40A/B/C/D450-582[»]
2GYKX-ray1.60B/F450-582[»]
2GZEX-ray1.80B450-582[»]
2GZFX-ray1.75B450-582[»]
2GZGX-ray1.70B450-582[»]
2GZIX-ray1.70B450-582[»]
2GZJX-ray1.60B/F450-582[»]
2IVZX-ray2.00E/F/G/H32-47[»]
2K5XNMR-B450-582[»]
2VLNX-ray1.60B450-582[»]
2VLOX-ray1.80B450-582[»]
2VLPX-ray2.00B450-582[»]
2VLQX-ray1.60B450-582[»]
2WPTX-ray1.78B450-582[»]
3O0EX-ray3.01L/M/N/O/P/Q2-18[»]
4JMLX-ray2.00E32-47[»]
DisProtiDP00342.
ProteinModelPortaliP09883.
SMRiP09883. Positions 82-582.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-16992N.
IntActiP09883. 3 interactions.
MINTiMINT-1771504.

Protein family/group databases

TCDBi1.C.1.4.1. the channel-forming colicin (colicin) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi7377410.
KEGGipg:7377410.

Miscellaneous databases

EvolutionaryTraceiP09883.

Family and domain databases

Gene3Di1.10.287.620. 1 hit.
3.90.540.10. 1 hit.
InterProiIPR024575. Cloacin_colicin_fam.
IPR024622. Colicin/pyocin_DNase_dom.
IPR024566. Colicin_R_dom.
IPR003615. HNH_nuc.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view]
PfamiPF03515. Cloacin. 1 hit.
PF12639. Colicin-DNase. 1 hit.
PF11570. E2R135. 1 hit.
[Graphical view]
PRINTSiPR01295. CLOACIN.
SMARTiSM00507. HNHc. 1 hit.
[Graphical view]
SUPFAMiSSF69369. SSF69369. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. James R.
    Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequences from the colicin E5, E6 and E9 operons: presence of a degenerate transposon-like structure in the ColE9-J plasmid."
    Lau P.C.K., Condie J.A.
    Mol. Gen. Genet. 217:269-277(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 378-582.
  3. "Complete nucleotide sequence of the colicin E9 (cei) gene."
    Eaton T., James R.
    Nucleic Acids Res. 17:1761-1761(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 509-582.

Entry informationi

Entry nameiCEA9_ECOLX
AccessioniPrimary (citable) accession number: P09883
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 30, 1989
Last sequence update: September 30, 1996
Last modified: March 31, 2015
This is version 100 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.