Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P09883

- CEA9_ECOLX

UniProt

P09883 - CEA9_ECOLX

Protein

Colicin-E9

Gene

col

Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 4 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This plasmid-coded bactericidal protein is an endonuclease active on both single- and double-stranded DNA but with undefined specificity.
    Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi550 – 5501ZincBy similarity
    Metal bindingi575 – 5751ZincBy similarity
    Metal bindingi579 – 5791ZincBy similarity

    GO - Molecular functioni

    1. endonuclease activity Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. cytolysis Source: UniProtKB-KW
    2. defense response to bacterium Source: UniProtKB-KW
    3. pathogenesis Source: InterPro

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial, Bacteriocin, Endonuclease, Hydrolase, Nuclease

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    TCDBi1.C.1.4.1. the channel-forming colicin (colicin) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Colicin-E9 (EC:3.1.-.-)
    Gene namesi
    Name:col
    Synonyms:cei
    Encoded oniPlasmid ColE90 Publication
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    Subcellular locationi

    GO - Cellular componenti

    1. extrachromosomal circular DNA Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 582582Colicin-E9PRO_0000218684Add
    BLAST

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    tolBP0A8555EBI-1029888,EBI-7180728From a different organism.

    Protein-protein interaction databases

    DIPiDIP-16992N.
    IntActiP09883. 3 interactions.
    MINTiMINT-1771504.

    Structurei

    Secondary structure

    1
    582
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi38 – 403
    Helixi452 – 4543
    Beta strandi457 – 4604
    Helixi470 – 4734
    Beta strandi476 – 4783
    Helixi484 – 4907
    Beta strandi494 – 4974
    Helixi498 – 51114
    Helixi513 – 5164
    Beta strandi517 – 5193
    Helixi521 – 5288
    Helixi537 – 5393
    Beta strandi548 – 5536
    Helixi555 – 5573
    Beta strandi561 – 5633
    Helixi564 – 5663
    Beta strandi567 – 5704
    Helixi572 – 5798

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BXIX-ray2.05B449-582[»]
    1EMVX-ray1.70B450-582[»]
    1FR2X-ray1.60B450-582[»]
    1FSJX-ray1.80B/C/D/E450-582[»]
    1V13X-ray2.00A/B450-582[»]
    1V14X-ray2.90A/B/C/D450-582[»]
    1V15X-ray2.40A/B/C/D450-582[»]
    2GYKX-ray1.60B/F450-582[»]
    2GZEX-ray1.80B450-582[»]
    2GZFX-ray1.75B450-582[»]
    2GZGX-ray1.70B450-582[»]
    2GZIX-ray1.70B450-582[»]
    2GZJX-ray1.60B/F450-582[»]
    2IVZX-ray2.00E/F/G/H32-47[»]
    2K5XNMR-B450-582[»]
    2VLNX-ray1.60B450-582[»]
    2VLOX-ray1.80B450-582[»]
    2VLPX-ray2.00B450-582[»]
    2VLQX-ray1.60B450-582[»]
    2WPTX-ray1.78B450-582[»]
    3O0EX-ray3.01L/M/N/O/P/Q2-18[»]
    4JMLX-ray2.00E32-47[»]
    DisProtiDP00342.
    ProteinModelPortaliP09883.
    SMRiP09883. Positions 82-582.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP09883.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the colicin/pyosin nuclease family.Curated

    Family and domain databases

    Gene3Di1.10.287.620. 1 hit.
    3.90.540.10. 1 hit.
    InterProiIPR024575. Cloacin_colicin_fam.
    IPR024622. Colicin/pyocin_DNase_dom.
    IPR024566. Colicin_R_dom.
    IPR003615. HNH_nuc.
    IPR016128. Pyosin/cloacin_T_dom.
    [Graphical view]
    PfamiPF03515. Cloacin. 1 hit.
    PF12639. Colicin-DNase. 1 hit.
    PF11570. E2R135. 1 hit.
    [Graphical view]
    PRINTSiPR01295. CLOACIN.
    SMARTiSM00507. HNHc. 1 hit.
    [Graphical view]
    SUPFAMiSSF69369. SSF69369. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P09883-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGGDGRGHN TGAHSTSGNI NGGPTGIGVS GGASDGSGWS SENNPWGGGS    50
    GSGIHWGGGS GRGNGGGNGN SGGGSGTGGN LSAVAAPVAF GFPALSTPGA 100
    GGLAVSISAS ELSAAIAGII AKLKKVNLKF TPFGVVLSSL IPSEIAKDDP 150
    NMMSKIVTSL PADDITESPV SSLPLDKATV NVNVRVVDDV KDERQNISVV 200
    SGVPMSVPVV DAKPTERPGV FTASIPGAPV LNISVNDSTP AVQTLSPGVT 250
    NNTDKDVRPA GFTQGGNTRD AVIRFPKDSG HNAVYVSVSD VLSPDQVKQR 300
    QDEENRRQQE WDATHPVEAA ERNYERARAE LNQANEDVAR NQERQAKAVQ 350
    VYNSRKSELD AANKTLADAI AEIKQFNRFA HDPMAGGHRM WQMAGLKAQR 400
    AQTDVNNKQA AFDAAAKEKS DADAALSAAQ ERRKQKENKE KDAKDKLDKE 450
    SKRNKPGKAT GKGKPVGDKW LDDAGKDSGA PIPDRIADKL RDKEFKSFDD 500
    FRKAVWEEVS KDPELSKNLN PSNKSSVSKG YSPFTPKNQQ VGGRKVYELH 550
    HDKPISQGGE VYDMDNIRVT TPKRHIDIHR GK 582
    Length:582
    Mass (Da):61,587
    Last modified:October 1, 1996 - v4
    Checksum:i47A71B57B45AFDD9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti511 – 5111K → R(PubMed:2646600)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12591 Genomic DNA. Translation: CAA31104.1.
    X15858 Genomic DNA. Translation: CAA33862.1.
    PIRiPQ0032.
    RefSeqiWP_012644886.1. NC_011977.1.
    YP_002533537.1. NC_011977.1.

    Genome annotation databases

    GeneIDi7377410.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12591 Genomic DNA. Translation: CAA31104.1 .
    X15858 Genomic DNA. Translation: CAA33862.1 .
    PIRi PQ0032.
    RefSeqi WP_012644886.1. NC_011977.1.
    YP_002533537.1. NC_011977.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BXI X-ray 2.05 B 449-582 [» ]
    1EMV X-ray 1.70 B 450-582 [» ]
    1FR2 X-ray 1.60 B 450-582 [» ]
    1FSJ X-ray 1.80 B/C/D/E 450-582 [» ]
    1V13 X-ray 2.00 A/B 450-582 [» ]
    1V14 X-ray 2.90 A/B/C/D 450-582 [» ]
    1V15 X-ray 2.40 A/B/C/D 450-582 [» ]
    2GYK X-ray 1.60 B/F 450-582 [» ]
    2GZE X-ray 1.80 B 450-582 [» ]
    2GZF X-ray 1.75 B 450-582 [» ]
    2GZG X-ray 1.70 B 450-582 [» ]
    2GZI X-ray 1.70 B 450-582 [» ]
    2GZJ X-ray 1.60 B/F 450-582 [» ]
    2IVZ X-ray 2.00 E/F/G/H 32-47 [» ]
    2K5X NMR - B 450-582 [» ]
    2VLN X-ray 1.60 B 450-582 [» ]
    2VLO X-ray 1.80 B 450-582 [» ]
    2VLP X-ray 2.00 B 450-582 [» ]
    2VLQ X-ray 1.60 B 450-582 [» ]
    2WPT X-ray 1.78 B 450-582 [» ]
    3O0E X-ray 3.01 L/M/N/O/P/Q 2-18 [» ]
    4JML X-ray 2.00 E 32-47 [» ]
    DisProti DP00342.
    ProteinModelPortali P09883.
    SMRi P09883. Positions 82-582.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-16992N.
    IntActi P09883. 3 interactions.
    MINTi MINT-1771504.

    Protein family/group databases

    TCDBi 1.C.1.4.1. the channel-forming colicin (colicin) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 7377410.

    Miscellaneous databases

    EvolutionaryTracei P09883.

    Family and domain databases

    Gene3Di 1.10.287.620. 1 hit.
    3.90.540.10. 1 hit.
    InterProi IPR024575. Cloacin_colicin_fam.
    IPR024622. Colicin/pyocin_DNase_dom.
    IPR024566. Colicin_R_dom.
    IPR003615. HNH_nuc.
    IPR016128. Pyosin/cloacin_T_dom.
    [Graphical view ]
    Pfami PF03515. Cloacin. 1 hit.
    PF12639. Colicin-DNase. 1 hit.
    PF11570. E2R135. 1 hit.
    [Graphical view ]
    PRINTSi PR01295. CLOACIN.
    SMARTi SM00507. HNHc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69369. SSF69369. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. James R.
      Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Nucleotide sequences from the colicin E5, E6 and E9 operons: presence of a degenerate transposon-like structure in the ColE9-J plasmid."
      Lau P.C.K., Condie J.A.
      Mol. Gen. Genet. 217:269-277(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 378-582.
    3. "Complete nucleotide sequence of the colicin E9 (cei) gene."
      Eaton T., James R.
      Nucleic Acids Res. 17:1761-1761(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 509-582.

    Entry informationi

    Entry nameiCEA9_ECOLX
    AccessioniPrimary (citable) accession number: P09883
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 97 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Plasmid

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3