P09881 (IMM8_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Colicin-E8 immunity protein

Alternative name(s):
ImmE8
Microcin-E8 immunity protein

Gene names

Name:	imm
Synonyms:	ceiE8

Encoded on

Plasmid ColE8

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	85 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein is able to protect a cell, which harbors the plasmid ColE8 encoding colicin E8, against colicin E8, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity.
Sequence similarities	Belongs to the colicins ColE2/ColE8/ColE9 and pyocins S1/S2 family.

Ontologies

Keywords
Biological process	Bacteriocin immunity
Technical term	3D-structure Plasmid
Gene Ontology (GO)
Biological_process	bacteriocin immunity Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	toxin binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 85

85

Colicin-E8 immunity protein

PRO_0000218709

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

85

Helix Strand Turn

Details...

Turn

7 – 9

3

Helix

12 – 24

13

Beta strand

26 – 29

4

Helix

30 – 41

12

Turn

46 – 49

4

Helix

50 – 53

4

Helix

63 – 76

14

Sequences

	Sequence		Length	Mass (Da)
	P09881 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: E31C481EFF6B4A6E	FASTA	85	9,623
	10 20 30 40 50 60 MELKNSISDY TETEFKKIIE DIINCEGDEK KQDDNLEHFI SVTEHPSGSD LIYYPEGNND 70 80 GSPEAVIKEI KEWRAANGKS GFKQG

References

[1]	"Nucleotide sequences from the colicin E8 operon: homology with plasmid ColE2-P9." Uchimura T., Lau P.C.K. Mol. Gen. Genet. 209:489-493(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Colicin E8, a DNase which indicates an evolutionary relationship between colicins E2 and E3." Toba M., Masaki H., Ohta T. J. Bacteriol. 170:3237-3242(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	Videler H., Boetzel R., Osborne M.J., Williams G., Lian L.Y., James R., Kleanthous C., Moore G.R. Submitted (JAN-1997) to the PDB data bank Cited for: STRUCTURE BY NMR.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M21404 Genomic DNA. Translation: AAA23074.1.
X06119 Genomic DNA. Translation: CAA29492.1.

PIR

IMECE8. B28184.

RefSeq

YP_002993420.1. NC_012882.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GXG	NMR	-	A	1-85	[»]
1GXH	NMR	-	A	1-85	[»]

ProteinModelPortal

P09881.

SMR

P09881. Positions 1-85.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

8094683.

Phylogenomic databases

ProtClustDB

CLSK2549962.

Family and domain databases

Gene3D

1.10.1200.20. 1 hit.

InterPro

IPR023802. Colicin/pyocin_immunity_dom.
IPR000290. Colicin_pyocin.
[Graphical view]

Pfam

PF01320. Colicin_Pyocin. 1 hit.
[Graphical view]

PRINTS

PR01299. PYOCIN.

ProDom

PD007225. Colicin_pyocin. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF47345. Colicin_pyocin. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P09881.

Entry information

Entry name

IMM8_ECOLX

Accession

Primary (citable) accession number: P09881

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program