Reviewed, UniProtKB/Swiss-Prot P09870 (CLOS_CLOHI)

Names and origin

Protein names

Recommended name:
    Clostripain
    EC=3.4.22.8
Alternative name(s):
    Clostridiopeptidase B
Cleaved into the following 2 chains:
    1- Recommended name:
            Clostripain light chain
    2- Recommended name:
            Clostripain heavy chain

Gene names

Name:

cloSI

Organism

Clostridium histolyticum

Taxonomic identifier

1498 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cysteine endopeptidase with strict specificity.
Catalytic activity	Preferential cleavage: Arg-|-Xaa, including Arg-|-Pro bond, but not Lys-|-Xaa.
Subunit structure	Heterodimer of a light chain and an heavy chain held together by strong noncovalent forces rather than by intramolecular disulfide bridges.
Sequence similarities	Belongs to the peptidase C11 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Propeptide	28 – 50	23	Potential		PRO_0000028511
	Chain	51 – 181	131	Clostripain light chain		PRO_0000028512
	Propeptide	182 – 190	9	Linker		PRO_0000028513
	Chain	191 – 526	336	Clostripain heavy chain		PRO_0000028514
Sites
	Active site	231	1
Experimental info
	Sequence conflict	127	1	R → NQL AA sequence Ref.2
	Sequence conflict	176 – 179	4	HGGG → GDGH AA sequence Ref.2
	Sequence conflict	197	1	S → H AA sequence Ref.3
	Sequence conflict	213	1	I → L AA sequence Ref.3
	Sequence conflict	216	1	H → T AA sequence Ref.3
	Sequence conflict	232	1	L → M AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P09870-1 [UniParc]. Last modified July 1, 1993. Version 2. Checksum: E151372FF6C95BE7	FASTA	526	59,733
	10 20 30 40 50 60 MLRRKVSTLL MTALITTSFL NSKPVYANPV TKSKDNNLKE VQQVTSKSNK NKNQKVTIMY 70 80 90 100 110 120 YCDADNNLEG SLLNDIEEMK TGYKDSPNLN LIALVDRSPR YSSDEKVLGE DFSDTRLYKI 130 140 150 160 170 180 EHNKANRLDG KNEFPEISTT SKYEANMGDP EVLKKFIDYC KSNYEADKYV LIMANHGGGA 190 200 210 220 230 240 REKSNPRLNR AICWDDSNLD KNGEADCLYM GEISDHLTEK QSVDLLAFDA CLMGTAEVAY 250 260 270 280 290 300 QYRPGNGGFS ADTLVASSPV VWGPGFKYDK IFDRIKAGGG TNNEDDLTLG GKEQNFDPAT 310 320 330 340 350 360 ITNEQLGALF VEEQRDSTHA NGRYDQHLSF YDLKKAESVK RAIDNLAVNL SNENKKSEIE 370 380 390 400 410 420 KLRGSGIHTD LMHYFDEYSE GEWVEYPYFD VYDLCEKINK SENFSSKTKD LASNAMNKLN 430 440 450 460 470 480 EMIVYSFGDP SNNFKEGKNG LSIFLPNGDK KYSTYYTSTK IPHWTMQSWY NSIDTVKYGL 490 500 510 520 NPYGKLSWCK DGQDPEINKV GNWFELLDSW FDKTNDVTGG VNHYQW

References

[1]	"The heterodimeric protease clostripain from Clostridium histolyticum is encoded by a single gene." Dargatz H., Diefenthal T., Witte V., Reipen G., von Wettstein D. Mol. Gen. Genet. 240:140-145(1993) [PubMed: 8341259] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Primary structure of alpha-clostripain light chain." Gilles A.M., Lecroisey A., Keil B. Eur. J. Biochem. 145:469-476(1984) [PubMed: 6391922] [Abstract] Cited for: PROTEIN SEQUENCE OF 51-181.
[3]	"Amino-acid sequences of the active-site sulfhydryl peptide and other thiol peptides from the cysteine proteinase alpha-clostripain." Gilles A.M., de Wolf A., Keil B. Eur. J. Biochem. 130:473-479(1983) [PubMed: 6337850] [Abstract] Cited for: PRELIMINARY PROTEIN SEQUENCE OF 51-73 AND 191-232.

Web resources

Worthington enzyme manual

Cross-references

Sequence databases
EMBLGenBankDDBJ	X63673 Genomic DNA. Translation: CAA45212.1.
PIR	A29174. A29175. B29175. S35190.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	C11.001.
Enzyme and pathway databases
BRENDA	3.4.22.8. 39549.
Family and domain databases
InterPro	IPR014173. Pept_C11_CLOspp. IPR005077. Peptidase_C11. [Graphical view]
Pfam	PF03415. Peptidase_C11. 1 hit. [Graphical view]
TIGRFAMs	TIGR02806. clostrip. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CLOS_CLOHI

Accession

Primary (citable) accession number: P09870
Secondary accession number(s): P09869

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1993
Last modified:	May 5, 2009
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)