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Protein

Ras GTPase-activating protein 1

Gene

RASA1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGTPase activation

Names & Taxonomyi

Protein namesi
Recommended name:
Ras GTPase-activating protein 1
Short name:
GAP
Short name:
GTPase-activating protein
Short name:
RasGAP
Alternative name(s):
Ras p21 protein activator
p120GAP
Gene namesi
Name:RASA1
Synonyms:RASA
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000566351 – 1044Ras GTPase-activating protein 1Add BLAST1044

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei612PhosphotyrosineBy similarity1
Modified residuei828PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by SRC and LCK. The phosphorylation SRC inhibits its ability to stimulate the Ras-GTPase activity, whereas phosphorylation by LCK does not display any effect on stimulation activity (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP09851.
PRIDEiP09851.

PTM databases

iPTMnetiP09851.

Interactioni

Subunit structurei

Interacts with SQSTM1. Interacts with SPSB1. Interaction with SPSB1 does not promote degradation. Interacts with CAV2 (tyrosine phosphorylated form). Directly interacts with NCK1. Interacts with PDGFRB (tyrosine phosphorylated) (By similarity). Interacts (via SH2 domain) with the 'Tyr-9' phosphorylated form of PDPK1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi159320. 2 interactors.
MINTiMINT-1517968.
STRINGi9913.ENSBTAP00000012583.

Structurei

3D structure databases

ProteinModelPortaliP09851.
SMRiP09851.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini178 – 269SH2 1PROSITE-ProRule annotationAdd BLAST92
Domaini276 – 338SH3PROSITE-ProRule annotationAdd BLAST63
Domaini348 – 438SH2 2PROSITE-ProRule annotationAdd BLAST91
Domaini471 – 574PHPROSITE-ProRule annotationAdd BLAST104
Domaini578 – 673C2PROSITE-ProRule annotationAdd BLAST96
Repeati646 – 664Add BLAST19
Repeati665 – 683Add BLAST19
Domaini745 – 939Ras-GAPPROSITE-ProRule annotationAdd BLAST195

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 160HydrophobicAdd BLAST160

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi23 – 26Poly-Ala4
Compositional biasi45 – 48Poly-Ala4
Compositional biasi100 – 106Poly-Ala7
Compositional biasi131 – 138Poly-Pro8
Compositional biasi160 – 165Poly-Glu6

Keywords - Domaini

Repeat, SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG3508. Eukaryota.
ENOG410XPU1. LUCA.
HOGENOMiHOG000007794.
HOVERGENiHBG057470.
InParanoidiP09851.
KOiK04352.

Family and domain databases

CDDicd10354. SH2_Cterm_RasGAP. 1 hit.
cd10353. SH2_Nterm_RasGAP. 1 hit.
cd11788. SH3_RasGAP. 1 hit.
Gene3Di2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.30.505.10. 2 hits.
InterProiView protein in InterPro
IPR000008. C2_dom.
IPR035892. C2_domain_sf.
IPR028554. p120-RasGAP.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR035842. RasGAP_C_SH2.
IPR023152. RasGAP_CS.
IPR001936. RasGAP_dom.
IPR035841. RasGAP_N_SH2.
IPR035652. RasGAP_SH3.
IPR008936. Rho_GTPase_activation_prot.
IPR000980. SH2.
IPR036860. SH2_dom_sf.
IPR036028. SH3-like_dom.
IPR001452. SH3_domain.
PANTHERiPTHR10194:SF19. PTHR10194:SF19. 1 hit.
PfamiView protein in Pfam
PF00168. C2. 1 hit.
PF00169. PH. 1 hit.
PF00616. RasGAP. 1 hit.
PF00017. SH2. 2 hits.
PF00018. SH3_1. 1 hit.
PRINTSiPR00401. SH2DOMAIN.
SMARTiView protein in SMART
SM00239. C2. 1 hit.
SM00233. PH. 1 hit.
SM00323. RasGAP. 1 hit.
SM00252. SH2. 2 hits.
SM00326. SH3. 1 hit.
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF55550. SSF55550. 2 hits.
PROSITEiView protein in PROSITE
PS50004. C2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
PS50001. SH2. 2 hits.
PS50002. SH3. 1 hit.

Sequencei

Sequence statusi: Complete.

P09851-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMAAEAGGEE GGPVTAGAAG GGAAAASGAY PAVCRVKIPA ALPVAAAAPF
60 70 80 90 100
PGLAEAGVAA TLGGGAALGS GFLGAGSVAG TPGGVGLSAG GAAAGVAGVA
110 120 130 140 150
AAAAGAGGEM AFAKGTTSLP TETFGAGGGF PPLPPPPPQL PTLGAGLGTV
160 170 180 190 200
DEGDSLDGPE YEEEEVAIPL TAPPTNQWYH GKLDRTIAEE RLRQAGKSGS
210 220 230 240 250
YLIRESDRRP GSFVLSFLSQ TNVVNHFRII AMCGDYYIGG RRFSSLSDLI
260 270 280 290 300
GYYSHVSCLL KGEKLLYPVA PPEPVEDRRR VRAILPYTKV PDTDEISFLK
310 320 330 340 350
GDMFIVHNEL EDGWMWVTNL RTDEQGLIVE DLVEEVGREE DPHEGKIWFH
360 370 380 390 400
GKISKQEAYN LLMTVGQACS FLVRPSDNTP GDYSLYFRTS ENIQRFKICP
410 420 430 440 450
TPNNQFMMGG RYYNSIGDII DHYRKEQIVE GYYLKEPVPM QDQEQVLNDA
460 470 480 490 500
VDGKEIYNTI RRKTKDAFYK NIVKKGYLLK KGKGKRWKNL YFILEGSDAQ
510 520 530 540 550
LIYFESEKRA TKPKGLIDLS VCSVYVVHDS LFGRPNCFQI VVQHFSEEHY
560 570 580 590 600
IFYFAGETPE QAEDWMKGLQ AFCNLRKSSP GTSNKRLRQV SSLILHIEEA
610 620 630 640 650
HKLPVKHFTN PYCNIYLNSV QVAKTHAREG QNPVWSEEFV FDDLPPDINR
660 670 680 690 700
FEITLSNKTK KSKDPDILFM RCQLSRLQKG HATDEWFLLS SHIPLKGIEP
710 720 730 740 750
GSLRVRARYS MEKIMPEEEY SEFKELILQK ELHVVYALSH VCGQDRTLLA
760 770 780 790 800
SILLKIFLHE KLESLLLCTL NDREISMEDE ATTLFRATTL ASTLMEQSMK
810 820 830 840 850
ATATQFVHHA LKDSILRIME SKQSCELSPS KLEKNEDVNT NLAHLLNILS
860 870 880 890 900
ELVEKIFMAS EILPPTLRYI YGCLQKSVQH KWPTNTTMRT RVVSGFVFLR
910 920 930 940 950
LICPAILNPR MFNIISDSPS PIAARTLTLV AKSVQNLANL VEFGAKEPYM
960 970 980 990 1000
EGVNPFIKSN KHRMIMFLDE LGNVPELPDT TEHSRTDLCR DLAALHEICV
1010 1020 1030 1040
AHSDELRTLS NERGAQQHVL KKLLAITELL QQKQNQYTKT NDVR
Length:1,044
Mass (Da):115,761
Last modified:July 1, 1989 - v1
Checksum:i9B15D1223FA5A61A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12602 mRNA. Translation: CAA31122.1.
PIRiS01966.
RefSeqiNP_776874.1. NM_174449.2.
UniGeneiBt.102247.

Genome annotation databases

GeneIDi282032.
KEGGibta:282032.

Similar proteinsi

Entry informationi

Entry nameiRASA1_BOVIN
AccessioniPrimary (citable) accession number: P09851
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 25, 2017
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome