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Protein

Ras GTPase-activating protein 1

Gene

RASA1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Names & Taxonomyi

Protein namesi
Recommended name:
Ras GTPase-activating protein 1
Short name:
GAP
Short name:
GTPase-activating protein
Short name:
RasGAP
Alternative name(s):
Ras p21 protein activator
p120GAP
Gene namesi
Name:RASA1
Synonyms:RASA
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10441044Ras GTPase-activating protein 1PRO_0000056635Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei612 – 6121PhosphotyrosineBy similarity
Modified residuei828 – 8281PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by SRC and LCK. The phosphorylation SRC inhibits its ability to stimulate the Ras-GTPase activity, whereas phosphorylation by LCK does not display any effect on stimulation activity (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP09851.
PRIDEiP09851.

PTM databases

iPTMnetiP09851.

Interactioni

Subunit structurei

Interacts with SQSTM1. Interacts with SPSB1. Interaction with SPSB1 does not promote degradation. Interacts with CAV2 (tyrosine phosphorylated form). Directly interacts with NCK1. Interacts with PDGFRB (tyrosine phosphorylated) (By similarity). Interacts (via SH2 domain) with the 'Tyr-9' phosphorylated form of PDPK1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi159320. 2 interactions.
MINTiMINT-1517968.
STRINGi9913.ENSBTAP00000012583.

Structurei

3D structure databases

ProteinModelPortaliP09851.
SMRiP09851. Positions 278-443, 715-1038.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini178 – 26992SH2 1PROSITE-ProRule annotationAdd
BLAST
Domaini276 – 33863SH3PROSITE-ProRule annotationAdd
BLAST
Domaini348 – 43891SH2 2PROSITE-ProRule annotationAdd
BLAST
Domaini471 – 574104PHPROSITE-ProRule annotationAdd
BLAST
Domaini578 – 67396C2PROSITE-ProRule annotationAdd
BLAST
Repeati646 – 66419Add
BLAST
Repeati665 – 68319Add
BLAST
Domaini745 – 939195Ras-GAPPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 160160HydrophobicAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi23 – 264Poly-Ala
Compositional biasi45 – 484Poly-Ala
Compositional biasi100 – 1067Poly-Ala
Compositional biasi131 – 1388Poly-Pro
Compositional biasi160 – 1656Poly-Glu

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Ras-GAP domain.PROSITE-ProRule annotation
Contains 2 SH2 domains.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG3508. Eukaryota.
ENOG410XPU1. LUCA.
HOGENOMiHOG000007794.
HOVERGENiHBG057470.
InParanoidiP09851.
KOiK04352.

Family and domain databases

Gene3Di1.10.506.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.30.505.10. 2 hits.
InterProiIPR000008. C2_dom.
IPR028554. p120-RasGAP.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR023152. RasGAP_CS.
IPR001936. RasGAP_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10194:SF19. PTHR10194:SF19. 1 hit.
PfamiPF00168. C2. 1 hit.
PF00169. PH. 1 hit.
PF00616. RasGAP. 1 hit.
PF00017. SH2. 2 hits.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
SMARTiSM00239. C2. 1 hit.
SM00233. PH. 1 hit.
SM00323. RasGAP. 1 hit.
SM00252. SH2. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF55550. SSF55550. 2 hits.
PROSITEiPS50004. C2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
PS50001. SH2. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09851-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMAAEAGGEE GGPVTAGAAG GGAAAASGAY PAVCRVKIPA ALPVAAAAPF
60 70 80 90 100
PGLAEAGVAA TLGGGAALGS GFLGAGSVAG TPGGVGLSAG GAAAGVAGVA
110 120 130 140 150
AAAAGAGGEM AFAKGTTSLP TETFGAGGGF PPLPPPPPQL PTLGAGLGTV
160 170 180 190 200
DEGDSLDGPE YEEEEVAIPL TAPPTNQWYH GKLDRTIAEE RLRQAGKSGS
210 220 230 240 250
YLIRESDRRP GSFVLSFLSQ TNVVNHFRII AMCGDYYIGG RRFSSLSDLI
260 270 280 290 300
GYYSHVSCLL KGEKLLYPVA PPEPVEDRRR VRAILPYTKV PDTDEISFLK
310 320 330 340 350
GDMFIVHNEL EDGWMWVTNL RTDEQGLIVE DLVEEVGREE DPHEGKIWFH
360 370 380 390 400
GKISKQEAYN LLMTVGQACS FLVRPSDNTP GDYSLYFRTS ENIQRFKICP
410 420 430 440 450
TPNNQFMMGG RYYNSIGDII DHYRKEQIVE GYYLKEPVPM QDQEQVLNDA
460 470 480 490 500
VDGKEIYNTI RRKTKDAFYK NIVKKGYLLK KGKGKRWKNL YFILEGSDAQ
510 520 530 540 550
LIYFESEKRA TKPKGLIDLS VCSVYVVHDS LFGRPNCFQI VVQHFSEEHY
560 570 580 590 600
IFYFAGETPE QAEDWMKGLQ AFCNLRKSSP GTSNKRLRQV SSLILHIEEA
610 620 630 640 650
HKLPVKHFTN PYCNIYLNSV QVAKTHAREG QNPVWSEEFV FDDLPPDINR
660 670 680 690 700
FEITLSNKTK KSKDPDILFM RCQLSRLQKG HATDEWFLLS SHIPLKGIEP
710 720 730 740 750
GSLRVRARYS MEKIMPEEEY SEFKELILQK ELHVVYALSH VCGQDRTLLA
760 770 780 790 800
SILLKIFLHE KLESLLLCTL NDREISMEDE ATTLFRATTL ASTLMEQSMK
810 820 830 840 850
ATATQFVHHA LKDSILRIME SKQSCELSPS KLEKNEDVNT NLAHLLNILS
860 870 880 890 900
ELVEKIFMAS EILPPTLRYI YGCLQKSVQH KWPTNTTMRT RVVSGFVFLR
910 920 930 940 950
LICPAILNPR MFNIISDSPS PIAARTLTLV AKSVQNLANL VEFGAKEPYM
960 970 980 990 1000
EGVNPFIKSN KHRMIMFLDE LGNVPELPDT TEHSRTDLCR DLAALHEICV
1010 1020 1030 1040
AHSDELRTLS NERGAQQHVL KKLLAITELL QQKQNQYTKT NDVR
Length:1,044
Mass (Da):115,761
Last modified:July 1, 1989 - v1
Checksum:i9B15D1223FA5A61A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12602 mRNA. Translation: CAA31122.1.
PIRiS01966.
RefSeqiNP_776874.1. NM_174449.2.
UniGeneiBt.102247.

Genome annotation databases

GeneIDi282032.
KEGGibta:282032.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12602 mRNA. Translation: CAA31122.1.
PIRiS01966.
RefSeqiNP_776874.1. NM_174449.2.
UniGeneiBt.102247.

3D structure databases

ProteinModelPortaliP09851.
SMRiP09851. Positions 278-443, 715-1038.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi159320. 2 interactions.
MINTiMINT-1517968.
STRINGi9913.ENSBTAP00000012583.

PTM databases

iPTMnetiP09851.

Proteomic databases

PaxDbiP09851.
PRIDEiP09851.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi282032.
KEGGibta:282032.

Organism-specific databases

CTDi5921.

Phylogenomic databases

eggNOGiKOG3508. Eukaryota.
ENOG410XPU1. LUCA.
HOGENOMiHOG000007794.
HOVERGENiHBG057470.
InParanoidiP09851.
KOiK04352.

Family and domain databases

Gene3Di1.10.506.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.30.505.10. 2 hits.
InterProiIPR000008. C2_dom.
IPR028554. p120-RasGAP.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR023152. RasGAP_CS.
IPR001936. RasGAP_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10194:SF19. PTHR10194:SF19. 1 hit.
PfamiPF00168. C2. 1 hit.
PF00169. PH. 1 hit.
PF00616. RasGAP. 1 hit.
PF00017. SH2. 2 hits.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
SMARTiSM00239. C2. 1 hit.
SM00233. PH. 1 hit.
SM00323. RasGAP. 1 hit.
SM00252. SH2. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF55550. SSF55550. 2 hits.
PROSITEiPS50004. C2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
PS50001. SH2. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Brain.

Entry informationi

Entry nameiRASA1_BOVIN
AccessioniPrimary (citable) accession number: P09851
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: July 6, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.