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P09849

- LPH_RABIT

UniProt

P09849 - LPH_RABIT

Protein

Lactase-phlorizin hydrolase

Gene

LCT

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    LPH splits lactose in the small intestine.

    Catalytic activityi

    Lactose + H2O = D-galactose + D-glucose.
    Glycosyl-N-acylsphingosine + H2O = N-acylsphingosine + a sugar.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei1063 – 10631Proton donorSequence Analysis
    Active sitei1271 – 12711Nucleophile1 PublicationPROSITE-ProRule annotation
    Active sitei1536 – 15361Proton donorSequence Analysis
    Active sitei1747 – 17471Nucleophile1 PublicationPROSITE-ProRule annotation

    GO - Molecular functioni

    1. glycosylceramidase activity Source: UniProtKB-EC
    2. lactase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lactase-phlorizin hydrolase
    Alternative name(s):
    Lactase-glycosylceramidase
    Including the following 2 domains:
    Phlorizin hydrolase (EC:3.2.1.62)
    Gene namesi
    Name:LCT
    Synonyms:LPH
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Add
    BLAST
    Propeptidei20 – 866847Beta-glucosidasePRO_0000011769Add
    BLAST
    Chaini867 – 19261060Lactase-phlorizin hydrolasePRO_0000011770Add
    BLAST

    Keywords - PTMi

    Zymogen

    Proteomic databases

    PRIDEiP09849.

    Expressioni

    Tissue specificityi

    Intestine.

    Interactioni

    Protein-protein interaction databases

    STRINGi9986.ENSOCUP00000015999.

    Structurei

    3D structure databases

    ProteinModelPortaliP09849.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini20 – 18821863ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1902 – 192625CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1883 – 190119HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati79 – 172941Add
    BLAST
    Repeati360 – 8454862Add
    BLAST
    Repeati881 – 13674873Add
    BLAST
    Repeati1375 – 18004264Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni79 – 180017224 X approximate repeatsAdd
    BLAST

    Domaini

    The sequence exhibits 4 regions (I-IV) of internal homology; therefore LPH might have evolved by two cycles of partial gene duplication.

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Keywords - Domaini

    Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2723.
    HOGENOMiHOG000024957.
    HOVERGENiHBG006290.

    Family and domain databases

    Gene3Di3.20.20.80. 4 hits.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 4 hits.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 4 hits.
    PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 2 hits.
    PS00653. GLYCOSYL_HYDROL_F1_2. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09849-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELFWSIVFT VLLSFSCRGS DWESDSNFIS AAGPLTTDLL LSLQYPQGNQ     50
    TSDFAAGGKD LYVCSQPLPA FLPEYFSSLR ASEITHYKVF LSWAQLLPAG 100
    HSGDPDGNAV RCYRQLLEAL RAAQLQPMVV LHHQHLPASS ALRSAVFADL 150
    FAEYATFAFH AFGDLVGVWL TFSDLEAAIR ELPQPESRAS RLQLLTEAHR 200
    KAYEIYHQKY AAQGGKVSVV LQAEEISELL LESSTSALAK DSIDFLSLDL 250
    SYECQSEMSL PEKLSKLQTI EPKVKVFIFT LRLQDCPSSR KSPASLLFSF 300
    IEAINKDQVL TLGFDVNAFL NCSSTSKKSI SCFLTDSLAL QTDHERAARN 350
    SAPVSTYQRV WEMFAHQPRA ERDAFLQDTF PQGFLWGVST GAFNVEGGWA 400
    EGGRGPSVWD QFGHLKAAQG QATPEVASDS YYKWASDVAL LRGLRAQVYK 450
    FSISWSRIFP MGRGSSPSPQ GVAYYNKLID SLLDSHIEPM ATLFHWDLPQ 500
    ALQDEGGWQN ESVVDAFVDY AAFCFSAFGN RVKLWVTFHE PWVMSYAGYG 550
    TGQHAPGISD PGIASFQVAH LVLKAHARTW HHYNSHHRPQ QQGRVGIVLN 600
    SDWAEPLSPE RPEDLAASER FLHFMLGWFA HPIFVDGDYP ATMKAQIQQR 650
    NEQCPSPVAQ LPEFTDTEKQ LLKGSADFLG LSHYTSRLIS KAPEDSCIPS 700
    YDTIGGFSQH TDPAWPQTSS PWIRVVPWGI RRLLQFVSLE YTKGKVPIYL 750
    AGNGMPIGES ENLLSDSLRV DYFNQYINEV LKAIKEDSVD VRSYIARSLM 800
    DGFEGPAGYS QRFGLYHVNF NESSKPRTPR KSAFLLTSII EKNGFLTKAV 850
    KQPLPPNSAH LPSKTRASAL PSEVPSKAKV VWEKFSNQTK FERDLFYHGT 900
    FRDDFLWGVS SSAYQIEGAW DADGKGPSIW DNFTHTPGNG VTDNSTGDIA 950
    CDSYNQLDAD LNVLRALKVK AYRFSLSWSR IFPTGTNSSI NSHGVDYYNR 1000
    LIDGLLASDI FPMVTLFHWD LPQALQDIGG WENPSLIDLF DSYADYCFQT 1050
    FGDRVKFWIT FNEPTYYSWW SYGSGTFPPN VNDPGWAPYR ISHALIKAHA 1100
    RVYHTYDEKY RQSQNGVISL SLVAQWAEPK SPDVLRDVEA ADRKMQFTLG 1150
    WYAHPIFKTG DYPDAMKWKV GNRSELQHLA TSRLPSFTEE EKSYIRGTAD 1200
    VFCLNTYSSK IVQHKTPALN PPSYEDDQEL AEEEDTSWPT TAMNRAASFG 1250
    MRRLLNWIKE EYGDIPIYIT ENGVGLTNPR LEDIDRIFYY KTYINEALKA 1300
    YRLDGVNLRG YFAWSLMDNF EWLQGYTIKF GLYHVDFENV NRPRTARISA 1350
    SYYTELITNN GMPLPSEDEF VYGQFPEGFV WSTSTAAFQI EGAWRADGKG 1400
    LGIWDTFTHT RLKIENDDIA DVACDSYHKI SEDVVALQNL AVTHYRFSIS 1450
    WSRILPDGTT NYINEAGLNY YVRLIDALLA ANIKPQVTMY HFDLPQALQD 1500
    VGGWENETIV QRFKEYADVL FQRLGDKVKF WITLNEPFVV AYHGYGTGLY 1550
    APGIYFRPGT APYIVGHNLI KAHAEAWHLY NDVYRASQGG VISITISSDW 1600
    AEPRDPSNQE DVEAAKRYVQ FMGGWFAHPI FKNGDYNEVM KTQIRERSLA 1650
    AGLNESRLPE FTESEKRRIN GTYDFFGFNH YTTVLAYNFN YPSIMSTVDA 1700
    DRGVASIVDR SWPGSGSYWL KMTPFGFRRI LNWIKEEYNN PPIYVTENGV 1750
    SHRGDSYLND TTRIYYLRSY INEALKAVQQ DKVDLRGYTV WTLMDNFEWY 1800
    TGFSDKFGLH FVNYSDPSLP RIPRESAKFY ASIVRCNGFP DPAEGPHPCL 1850
    LQPEDTDPTM SPVSQEEVQF LGLSLGSTEA ETALYVLFSL MLLGVCGLAF 1900
    LSYALCKSSK QRKKLSQQEL SPVSSF 1926
    Length:1,926
    Mass (Da):217,849
    Last modified:July 1, 1989 - v1
    Checksum:i2A21A7370D0CFC7A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07995 mRNA. Translation: CAA30802.1.
    PIRiS01169.
    RefSeqiNP_001095159.1. NM_001101689.1.
    UniGeneiOcu.7767.

    Genome annotation databases

    GeneIDi100009256.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07995 mRNA. Translation: CAA30802.1 .
    PIRi S01169.
    RefSeqi NP_001095159.1. NM_001101689.1.
    UniGenei Ocu.7767.

    3D structure databases

    ProteinModelPortali P09849.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9986.ENSOCUP00000015999.

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Proteomic databases

    PRIDEi P09849.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100009256.

    Organism-specific databases

    CTDi 100009256.

    Phylogenomic databases

    eggNOGi COG2723.
    HOGENOMi HOG000024957.
    HOVERGENi HBG006290.

    Family and domain databases

    Gene3Di 3.20.20.80. 4 hits.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 4 hits.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 4 hits.
    PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 2 hits.
    PS00653. GLYCOSYL_HYDROL_F1_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete primary structure of human and rabbit lactase-phlorizin hydrolase: implications for biosynthesis, membrane anchoring and evolution of the enzyme."
      Mantei N., Villa M., Enzler T., Wacker H., Boll W., James P., Hunziker W., Semenza G.
      EMBO J. 7:2705-2713(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Strain: New Zealand white.
    2. "Location of the two catalytic sites in intestinal lactase-phlorizin hydrolase. Comparison with sucrase-isomaltase and with other glycosidases, the membrane anchor of lactase-phlorizin hydrolase."
      Wacker H., Keller P., Falchetto R., Legler G., Semenza G.
      J. Biol. Chem. 267:18744-18752(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACTIVE SITES.

    Entry informationi

    Entry nameiLPH_RABIT
    AccessioniPrimary (citable) accession number: P09849
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3