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Protein

Lactase-phlorizin hydrolase

Gene

LCT

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

LPH splits lactose in the small intestine.

Catalytic activityi

Lactose + H2O = D-galactose + D-glucose.
Glycosyl-N-acylsphingosine + H2O = N-acylsphingosine + a sugar.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei1063 – 10631Proton donorSequence Analysis
Active sitei1271 – 12711NucleophilePROSITE-ProRule annotation1 Publication
Active sitei1536 – 15361Proton donorSequence Analysis
Active sitei1747 – 17471NucleophilePROSITE-ProRule annotation1 Publication

GO - Molecular functioni

  1. glycosylceramidase activity Source: UniProtKB-EC
  2. lactase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Lactase-phlorizin hydrolase
Alternative name(s):
Lactase-glycosylceramidase
Including the following 2 domains:
Phlorizin hydrolase (EC:3.2.1.62)
Gene namesi
Name:LCT
Synonyms:LPH
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 18821863ExtracellularSequence AnalysisAdd
BLAST
Transmembranei1883 – 190119HelicalSequence AnalysisAdd
BLAST
Topological domaini1902 – 192625CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Add
BLAST
Propeptidei20 – 866847Beta-glucosidasePRO_0000011769Add
BLAST
Chaini867 – 19261060Lactase-phlorizin hydrolasePRO_0000011770Add
BLAST

Keywords - PTMi

Zymogen

Proteomic databases

PRIDEiP09849.

Expressioni

Tissue specificityi

Intestine.

Interactioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000015999.

Structurei

3D structure databases

ProteinModelPortaliP09849.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati79 – 172941Add
BLAST
Repeati360 – 8454862Add
BLAST
Repeati881 – 13674873Add
BLAST
Repeati1375 – 18004264Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni79 – 180017224 X approximate repeatsAdd
BLAST

Domaini

The sequence exhibits 4 regions (I-IV) of internal homology; therefore LPH might have evolved by two cycles of partial gene duplication.

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000024957.
HOVERGENiHBG006290.
InParanoidiP09849.
KOiK01229.

Family and domain databases

Gene3Di3.20.20.80. 4 hits.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 4 hits.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 4 hits.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 2 hits.
PS00653. GLYCOSYL_HYDROL_F1_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09849-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELFWSIVFT VLLSFSCRGS DWESDSNFIS AAGPLTTDLL LSLQYPQGNQ
60 70 80 90 100
TSDFAAGGKD LYVCSQPLPA FLPEYFSSLR ASEITHYKVF LSWAQLLPAG
110 120 130 140 150
HSGDPDGNAV RCYRQLLEAL RAAQLQPMVV LHHQHLPASS ALRSAVFADL
160 170 180 190 200
FAEYATFAFH AFGDLVGVWL TFSDLEAAIR ELPQPESRAS RLQLLTEAHR
210 220 230 240 250
KAYEIYHQKY AAQGGKVSVV LQAEEISELL LESSTSALAK DSIDFLSLDL
260 270 280 290 300
SYECQSEMSL PEKLSKLQTI EPKVKVFIFT LRLQDCPSSR KSPASLLFSF
310 320 330 340 350
IEAINKDQVL TLGFDVNAFL NCSSTSKKSI SCFLTDSLAL QTDHERAARN
360 370 380 390 400
SAPVSTYQRV WEMFAHQPRA ERDAFLQDTF PQGFLWGVST GAFNVEGGWA
410 420 430 440 450
EGGRGPSVWD QFGHLKAAQG QATPEVASDS YYKWASDVAL LRGLRAQVYK
460 470 480 490 500
FSISWSRIFP MGRGSSPSPQ GVAYYNKLID SLLDSHIEPM ATLFHWDLPQ
510 520 530 540 550
ALQDEGGWQN ESVVDAFVDY AAFCFSAFGN RVKLWVTFHE PWVMSYAGYG
560 570 580 590 600
TGQHAPGISD PGIASFQVAH LVLKAHARTW HHYNSHHRPQ QQGRVGIVLN
610 620 630 640 650
SDWAEPLSPE RPEDLAASER FLHFMLGWFA HPIFVDGDYP ATMKAQIQQR
660 670 680 690 700
NEQCPSPVAQ LPEFTDTEKQ LLKGSADFLG LSHYTSRLIS KAPEDSCIPS
710 720 730 740 750
YDTIGGFSQH TDPAWPQTSS PWIRVVPWGI RRLLQFVSLE YTKGKVPIYL
760 770 780 790 800
AGNGMPIGES ENLLSDSLRV DYFNQYINEV LKAIKEDSVD VRSYIARSLM
810 820 830 840 850
DGFEGPAGYS QRFGLYHVNF NESSKPRTPR KSAFLLTSII EKNGFLTKAV
860 870 880 890 900
KQPLPPNSAH LPSKTRASAL PSEVPSKAKV VWEKFSNQTK FERDLFYHGT
910 920 930 940 950
FRDDFLWGVS SSAYQIEGAW DADGKGPSIW DNFTHTPGNG VTDNSTGDIA
960 970 980 990 1000
CDSYNQLDAD LNVLRALKVK AYRFSLSWSR IFPTGTNSSI NSHGVDYYNR
1010 1020 1030 1040 1050
LIDGLLASDI FPMVTLFHWD LPQALQDIGG WENPSLIDLF DSYADYCFQT
1060 1070 1080 1090 1100
FGDRVKFWIT FNEPTYYSWW SYGSGTFPPN VNDPGWAPYR ISHALIKAHA
1110 1120 1130 1140 1150
RVYHTYDEKY RQSQNGVISL SLVAQWAEPK SPDVLRDVEA ADRKMQFTLG
1160 1170 1180 1190 1200
WYAHPIFKTG DYPDAMKWKV GNRSELQHLA TSRLPSFTEE EKSYIRGTAD
1210 1220 1230 1240 1250
VFCLNTYSSK IVQHKTPALN PPSYEDDQEL AEEEDTSWPT TAMNRAASFG
1260 1270 1280 1290 1300
MRRLLNWIKE EYGDIPIYIT ENGVGLTNPR LEDIDRIFYY KTYINEALKA
1310 1320 1330 1340 1350
YRLDGVNLRG YFAWSLMDNF EWLQGYTIKF GLYHVDFENV NRPRTARISA
1360 1370 1380 1390 1400
SYYTELITNN GMPLPSEDEF VYGQFPEGFV WSTSTAAFQI EGAWRADGKG
1410 1420 1430 1440 1450
LGIWDTFTHT RLKIENDDIA DVACDSYHKI SEDVVALQNL AVTHYRFSIS
1460 1470 1480 1490 1500
WSRILPDGTT NYINEAGLNY YVRLIDALLA ANIKPQVTMY HFDLPQALQD
1510 1520 1530 1540 1550
VGGWENETIV QRFKEYADVL FQRLGDKVKF WITLNEPFVV AYHGYGTGLY
1560 1570 1580 1590 1600
APGIYFRPGT APYIVGHNLI KAHAEAWHLY NDVYRASQGG VISITISSDW
1610 1620 1630 1640 1650
AEPRDPSNQE DVEAAKRYVQ FMGGWFAHPI FKNGDYNEVM KTQIRERSLA
1660 1670 1680 1690 1700
AGLNESRLPE FTESEKRRIN GTYDFFGFNH YTTVLAYNFN YPSIMSTVDA
1710 1720 1730 1740 1750
DRGVASIVDR SWPGSGSYWL KMTPFGFRRI LNWIKEEYNN PPIYVTENGV
1760 1770 1780 1790 1800
SHRGDSYLND TTRIYYLRSY INEALKAVQQ DKVDLRGYTV WTLMDNFEWY
1810 1820 1830 1840 1850
TGFSDKFGLH FVNYSDPSLP RIPRESAKFY ASIVRCNGFP DPAEGPHPCL
1860 1870 1880 1890 1900
LQPEDTDPTM SPVSQEEVQF LGLSLGSTEA ETALYVLFSL MLLGVCGLAF
1910 1920
LSYALCKSSK QRKKLSQQEL SPVSSF
Length:1,926
Mass (Da):217,849
Last modified:July 1, 1989 - v1
Checksum:i2A21A7370D0CFC7A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07995 mRNA. Translation: CAA30802.1.
PIRiS01169.
RefSeqiNP_001095159.1. NM_001101689.1.
UniGeneiOcu.7767.

Genome annotation databases

GeneIDi100009256.
KEGGiocu:100009256.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07995 mRNA. Translation: CAA30802.1.
PIRiS01169.
RefSeqiNP_001095159.1. NM_001101689.1.
UniGeneiOcu.7767.

3D structure databases

ProteinModelPortaliP09849.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000015999.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PRIDEiP09849.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100009256.
KEGGiocu:100009256.

Organism-specific databases

CTDi100009256.

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000024957.
HOVERGENiHBG006290.
InParanoidiP09849.
KOiK01229.

Family and domain databases

Gene3Di3.20.20.80. 4 hits.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 4 hits.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 4 hits.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 2 hits.
PS00653. GLYCOSYL_HYDROL_F1_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete primary structure of human and rabbit lactase-phlorizin hydrolase: implications for biosynthesis, membrane anchoring and evolution of the enzyme."
    Mantei N., Villa M., Enzler T., Wacker H., Boll W., James P., Hunziker W., Semenza G.
    EMBO J. 7:2705-2713(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: New Zealand white.
  2. "Location of the two catalytic sites in intestinal lactase-phlorizin hydrolase. Comparison with sucrase-isomaltase and with other glycosidases, the membrane anchor of lactase-phlorizin hydrolase."
    Wacker H., Keller P., Falchetto R., Legler G., Semenza G.
    J. Biol. Chem. 267:18744-18752(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACTIVE SITES.

Entry informationi

Entry nameiLPH_RABIT
AccessioniPrimary (citable) accession number: P09849
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: February 4, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.