SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P09849

- LPH_RABIT

UniProt

P09849 - LPH_RABIT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Lactase-phlorizin hydrolase

Gene
LCT, LPH
Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

LPH splits lactose in the small intestine.

Catalytic activityi

Lactose + H2O = D-galactose + D-glucose.
Glycosyl-N-acylsphingosine + H2O = N-acylsphingosine + a sugar.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei1063 – 10631Proton donor Reviewed prediction
Active sitei1271 – 12711Nucleophile1 Publication
Active sitei1536 – 15361Proton donor Reviewed prediction
Active sitei1747 – 17471Nucleophile1 Publication

GO - Molecular functioni

  1. glycosylceramidase activity Source: UniProtKB-EC
  2. lactase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Lactase-phlorizin hydrolase
Alternative name(s):
Lactase-glycosylceramidase
Including the following 2 domains:
Phlorizin hydrolase (EC:3.2.1.62)
Gene namesi
Name:LCT
Synonyms:LPH
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 18821863Extracellular Reviewed predictionAdd
BLAST
Transmembranei1883 – 190119Helical; Reviewed predictionAdd
BLAST
Topological domaini1902 – 192625Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Add
BLAST
Propeptidei20 – 866847Beta-glucosidasePRO_0000011769Add
BLAST
Chaini867 – 19261060Lactase-phlorizin hydrolasePRO_0000011770Add
BLAST

Keywords - PTMi

Zymogen

Proteomic databases

PRIDEiP09849.

Expressioni

Tissue specificityi

Intestine.

Interactioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000015999.

Structurei

3D structure databases

ProteinModelPortaliP09849.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati79 – 172941Add
BLAST
Repeati360 – 8454862Add
BLAST
Repeati881 – 13674873Add
BLAST
Repeati1375 – 18004264Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni79 – 180017224 X approximate repeatsAdd
BLAST

Domaini

The sequence exhibits 4 regions (I-IV) of internal homology; therefore LPH might have evolved by two cycles of partial gene duplication.

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000024957.
HOVERGENiHBG006290.

Family and domain databases

Gene3Di3.20.20.80. 4 hits.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 4 hits.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 4 hits.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 2 hits.
PS00653. GLYCOSYL_HYDROL_F1_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09849-1 [UniParc]FASTAAdd to Basket

« Hide

MELFWSIVFT VLLSFSCRGS DWESDSNFIS AAGPLTTDLL LSLQYPQGNQ     50
TSDFAAGGKD LYVCSQPLPA FLPEYFSSLR ASEITHYKVF LSWAQLLPAG 100
HSGDPDGNAV RCYRQLLEAL RAAQLQPMVV LHHQHLPASS ALRSAVFADL 150
FAEYATFAFH AFGDLVGVWL TFSDLEAAIR ELPQPESRAS RLQLLTEAHR 200
KAYEIYHQKY AAQGGKVSVV LQAEEISELL LESSTSALAK DSIDFLSLDL 250
SYECQSEMSL PEKLSKLQTI EPKVKVFIFT LRLQDCPSSR KSPASLLFSF 300
IEAINKDQVL TLGFDVNAFL NCSSTSKKSI SCFLTDSLAL QTDHERAARN 350
SAPVSTYQRV WEMFAHQPRA ERDAFLQDTF PQGFLWGVST GAFNVEGGWA 400
EGGRGPSVWD QFGHLKAAQG QATPEVASDS YYKWASDVAL LRGLRAQVYK 450
FSISWSRIFP MGRGSSPSPQ GVAYYNKLID SLLDSHIEPM ATLFHWDLPQ 500
ALQDEGGWQN ESVVDAFVDY AAFCFSAFGN RVKLWVTFHE PWVMSYAGYG 550
TGQHAPGISD PGIASFQVAH LVLKAHARTW HHYNSHHRPQ QQGRVGIVLN 600
SDWAEPLSPE RPEDLAASER FLHFMLGWFA HPIFVDGDYP ATMKAQIQQR 650
NEQCPSPVAQ LPEFTDTEKQ LLKGSADFLG LSHYTSRLIS KAPEDSCIPS 700
YDTIGGFSQH TDPAWPQTSS PWIRVVPWGI RRLLQFVSLE YTKGKVPIYL 750
AGNGMPIGES ENLLSDSLRV DYFNQYINEV LKAIKEDSVD VRSYIARSLM 800
DGFEGPAGYS QRFGLYHVNF NESSKPRTPR KSAFLLTSII EKNGFLTKAV 850
KQPLPPNSAH LPSKTRASAL PSEVPSKAKV VWEKFSNQTK FERDLFYHGT 900
FRDDFLWGVS SSAYQIEGAW DADGKGPSIW DNFTHTPGNG VTDNSTGDIA 950
CDSYNQLDAD LNVLRALKVK AYRFSLSWSR IFPTGTNSSI NSHGVDYYNR 1000
LIDGLLASDI FPMVTLFHWD LPQALQDIGG WENPSLIDLF DSYADYCFQT 1050
FGDRVKFWIT FNEPTYYSWW SYGSGTFPPN VNDPGWAPYR ISHALIKAHA 1100
RVYHTYDEKY RQSQNGVISL SLVAQWAEPK SPDVLRDVEA ADRKMQFTLG 1150
WYAHPIFKTG DYPDAMKWKV GNRSELQHLA TSRLPSFTEE EKSYIRGTAD 1200
VFCLNTYSSK IVQHKTPALN PPSYEDDQEL AEEEDTSWPT TAMNRAASFG 1250
MRRLLNWIKE EYGDIPIYIT ENGVGLTNPR LEDIDRIFYY KTYINEALKA 1300
YRLDGVNLRG YFAWSLMDNF EWLQGYTIKF GLYHVDFENV NRPRTARISA 1350
SYYTELITNN GMPLPSEDEF VYGQFPEGFV WSTSTAAFQI EGAWRADGKG 1400
LGIWDTFTHT RLKIENDDIA DVACDSYHKI SEDVVALQNL AVTHYRFSIS 1450
WSRILPDGTT NYINEAGLNY YVRLIDALLA ANIKPQVTMY HFDLPQALQD 1500
VGGWENETIV QRFKEYADVL FQRLGDKVKF WITLNEPFVV AYHGYGTGLY 1550
APGIYFRPGT APYIVGHNLI KAHAEAWHLY NDVYRASQGG VISITISSDW 1600
AEPRDPSNQE DVEAAKRYVQ FMGGWFAHPI FKNGDYNEVM KTQIRERSLA 1650
AGLNESRLPE FTESEKRRIN GTYDFFGFNH YTTVLAYNFN YPSIMSTVDA 1700
DRGVASIVDR SWPGSGSYWL KMTPFGFRRI LNWIKEEYNN PPIYVTENGV 1750
SHRGDSYLND TTRIYYLRSY INEALKAVQQ DKVDLRGYTV WTLMDNFEWY 1800
TGFSDKFGLH FVNYSDPSLP RIPRESAKFY ASIVRCNGFP DPAEGPHPCL 1850
LQPEDTDPTM SPVSQEEVQF LGLSLGSTEA ETALYVLFSL MLLGVCGLAF 1900
LSYALCKSSK QRKKLSQQEL SPVSSF 1926
Length:1,926
Mass (Da):217,849
Last modified:July 1, 1989 - v1
Checksum:i2A21A7370D0CFC7A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X07995 mRNA. Translation: CAA30802.1.
PIRiS01169.
RefSeqiNP_001095159.1. NM_001101689.1.
UniGeneiOcu.7767.

Genome annotation databases

GeneIDi100009256.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X07995 mRNA. Translation: CAA30802.1 .
PIRi S01169.
RefSeqi NP_001095159.1. NM_001101689.1.
UniGenei Ocu.7767.

3D structure databases

ProteinModelPortali P09849.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9986.ENSOCUP00000015999.

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Proteomic databases

PRIDEi P09849.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100009256.

Organism-specific databases

CTDi 100009256.

Phylogenomic databases

eggNOGi COG2723.
HOGENOMi HOG000024957.
HOVERGENi HBG006290.

Family and domain databases

Gene3Di 3.20.20.80. 4 hits.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 4 hits.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 4 hits.
PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 2 hits.
PS00653. GLYCOSYL_HYDROL_F1_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete primary structure of human and rabbit lactase-phlorizin hydrolase: implications for biosynthesis, membrane anchoring and evolution of the enzyme."
    Mantei N., Villa M., Enzler T., Wacker H., Boll W., James P., Hunziker W., Semenza G.
    EMBO J. 7:2705-2713(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: New Zealand white.
  2. "Location of the two catalytic sites in intestinal lactase-phlorizin hydrolase. Comparison with sucrase-isomaltase and with other glycosidases, the membrane anchor of lactase-phlorizin hydrolase."
    Wacker H., Keller P., Falchetto R., Legler G., Semenza G.
    J. Biol. Chem. 267:18744-18752(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACTIVE SITES.

Entry informationi

Entry nameiLPH_RABIT
AccessioniPrimary (citable) accession number: P09849
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: February 19, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi