P09841 (HGLB_SCHMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Hemoglobinase EC=3.4.22.34 Alternative name(s): Antigen SM32
Organism	Schistosoma mansoni (Blood fluke)
Taxonomic identifier	6183 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Platyhelminthes › Trematoda › Digenea › Strigeidida › Schistosomatoidea › Schistosomatidae › Schistosoma

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protease is used by the parasite for degradation of the host globin.
Catalytic activity	Hydrolysis of proteins and small molecule substrates at -Asn-|-Xaa- bonds.
Sequence similarities	Belongs to the peptidase C13 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 31	12	Potential		PRO_0000026508
	Chain	32 – 291	260	Hemoglobinase		PRO_0000026509
	Propeptide	292 – 429	138	Potential		PRO_0000026510
Sites
	Active site	151	1	Potential
Experimental info
	Sequence conflict	91	1	P → L in AAA29895. Ref.2
	Sequence conflict	115	1	N → K in AAA29895. Ref.2
	Sequence conflict	216	1	S → P in AAA29895. Ref.2
	Sequence conflict	297	1	S → T in AAA29895. Ref.2
	Sequence conflict	302	1	P → S in AAA29895. Ref.2
	Sequence conflict	310	1	I → V in AAA29916. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P09841 [UniParc]. Last modified February 1, 1995. Version 3. Checksum: 333C9DA086C83B0F	FASTA	429	49,032
	10 20 30 40 50 60 MMLFSLFLIS ILHILLVKCQ LDTNYEVSDE TVSDNNKWAV LVAGSNGYPN YRHQADVCHA 70 80 90 100 110 120 YHVLRSKGIK PEHIITMMYD DIAYNLMNPF PGKLFNDYNH KDWYEGVVID YRGKNVNSKT 130 140 150 160 170 180 FLKVLKGDKS AGGKVLKSGK NDDVFIYFTD HGAPGLIAFP DDELYAKEFM STLKYLHSHK 190 200 210 220 230 240 RYSKLVIYIE ANESGSMFQQ ILPSNLSIYA TTAANSTECS YSTFCGDPTI TTCLADLYSY 250 260 270 280 290 300 NWIVDSQTHH LTQRTLDQQY KEVKRETDLS HVQRYGDTRM GKLYVSEFQG SRDKSSSEND 310 320 330 340 350 360 EPPMKPRHSI ASRDIPLHTL HRQIMMTNNA EDKSFLMQIL GLKLKRRDLI EDTMKLIVKV 370 380 390 400 410 420 MNNEEIPNTK ATIDQTLDCT ESVYEQFKSK CFTLQQAPEV GGHFSTLYNY CADGYTAETI NEAIIKICG

References

[1]	"Definition of the complete Schistosoma mansoni hemoglobinase mRNA sequence and gene expression in developing parasites." el Meanawy M.A., Aji T., Phillips N.F.B., Davis R.E., Salata R.A., Malchotra I., McClain D., Aikawa M., Davis A.H. Am. J. Trop. Med. Hyg. 43:67-78(1990) [PubMed: 2382765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 32-57.
[2]	"Primary structures of Sm31/32 diagnostic proteins of Schistosoma mansoni and their identification as proteases." Klinkert M.-Q., Felleisen R., Link G., Ruppel A., Beck E. Mol. Biochem. Parasitol. 33:113-122(1989) [PubMed: 2725581] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Cloning and gene expression of Schistosoma mansoni protease." Davis A.H., Nanduri J., Watson D.C. J. Biol. Chem. 262:12851-12855(1987) [PubMed: 3305515] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 77-429.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M21308 mRNA. Translation: AAA29895.1. M17423 mRNA. Translation: AAA29916.1.
PIR	A60145.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	3.4.22.34. 5608.
Family and domain databases
InterPro	IPR001096. Peptidase_C13. [Graphical view]
PANTHER	PTHR12000. Peptidase_C13. 1 hit.
Pfam	PF01650. Peptidase_C13. 1 hit. [Graphical view]
PIRSF	PIRSF019663. Legumain. 1 hit.
PRINTS	PR00776. HEMOGLOBNASE.
ProtoNet	Search...

Entry information

Entry name

HGLB_SCHMA

Accession

Primary (citable) accession number: P09841

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	February 1, 1995
Last modified:	November 16, 2011
This is version 64 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)