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Protein

DNA nucleotidylexotransferase

Gene

Dntt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator (PubMed:23856622). One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.Curated1 Publication

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).2 Publications

Cofactori

Mg2+1 PublicationNote: Can also utilize other divalent cations, such as Mn2+ and Co2+ (in vitro).Curated1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi343MagnesiumCombined sourcesCurated1
Metal bindingi345MagnesiumCombined sourcesCurated1
Metal bindingi434MagnesiumCombined sourcesCurated1

GO - Molecular functioni

GO - Biological processi

  • DNA metabolic process Source: MGI
  • DNA modification Source: UniProtKB-KW
  • response to ATP Source: Ensembl

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processTerminal addition
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BRENDAi2.7.7.31 3474

Names & Taxonomyi

Protein namesi
Recommended name:
DNA nucleotidylexotransferase (EC:2.7.7.312 Publications)
Alternative name(s):
Terminal addition enzyme
Terminal deoxynucleotidyltransferase1 Publication
Short name:
TDT
Short name:
Terminal transferase
Gene namesi
Name:Dntt
Synonyms:Tdt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:98659 Dntt

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi342H → A: Nearly abolishes enzyme activity. 1 Publication1
Mutagenesisi398L → A: Nearly abolishes enzyme activity. 1 Publication1
Mutagenesisi399D → A: Nearly abolishes enzyme activity. 1 Publication1
Mutagenesisi400H → A: Reduces enzyme activity. 1 Publication1
Mutagenesisi403K → A: Nearly abolishes enzyme activity. 1 Publication1
Mutagenesisi473D → A: Nearly abolishes enzyme activity. 1 Publication1
Mutagenesisi475H → A: Nearly abolishes enzyme activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002187921 – 530DNA nucleotidylexotransferaseAdd BLAST530

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei134PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP09838
PaxDbiP09838
PRIDEiP09838

PTM databases

iPTMnetiP09838
PhosphoSitePlusiP09838

Expressioni

Gene expression databases

BgeeiENSMUSG00000025014
CleanExiMM_DNTT
ExpressionAtlasiP09838 baseline and differential
GenevisibleiP09838 MM

Interactioni

Subunit structurei

Interacts with PRP19 and DNTTIP1. Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNA. Interacts with TRERF1.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000062078

Structurei

Secondary structure

1530
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi154 – 156Combined sources3
Helixi166 – 181Combined sources16
Helixi185 – 199Combined sources15
Helixi208 – 211Combined sources4
Helixi219 – 231Combined sources13
Helixi235 – 242Combined sources8
Helixi244 – 253Combined sources10
Helixi260 – 268Combined sources9
Helixi274 – 279Combined sources6
Helixi287 – 294Combined sources8
Helixi296 – 300Combined sources5
Helixi305 – 322Combined sources18
Beta strandi327 – 330Combined sources4
Helixi332 – 335Combined sources4
Beta strandi339 – 342Combined sources4
Beta strandi344 – 349Combined sources6
Helixi355 – 372Combined sources18
Beta strandi375 – 381Combined sources7
Helixi387 – 389Combined sources3
Beta strandi394 – 396Combined sources3
Beta strandi401 – 411Combined sources11
Helixi412 – 414Combined sources3
Beta strandi425 – 437Combined sources13
Helixi440 – 442Combined sources3
Helixi443 – 451Combined sources9
Helixi454 – 468Combined sources15
Beta strandi470 – 472Combined sources3
Beta strandi477 – 479Combined sources3
Turni480 – 482Combined sources3
Beta strandi504 – 506Combined sources3
Helixi511 – 518Combined sources8
Helixi525 – 527Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JMSX-ray2.36A130-530[»]
1KDHX-ray3.00A148-530[»]
1KEJX-ray3.00A148-530[»]
4I27X-ray2.60A132-530[»]
4I28X-ray2.15A132-530[»]
4I29X-ray2.20A132-530[»]
4I2AX-ray1.90A132-530[»]
4I2BX-ray2.20A132-530[»]
4I2CX-ray2.10A132-530[»]
4I2DX-ray2.30A132-530[»]
4I2EX-ray2.00A132-530[»]
4I2FX-ray2.10A132-530[»]
4I2GX-ray2.50A132-530[»]
4I2HX-ray2.75A132-530[»]
4I2IX-ray2.50A132-530[»]
4I2JX-ray2.70A132-530[»]
4IQTX-ray2.60A132-530[»]
4IQUX-ray2.40A132-530[»]
4IQVX-ray2.90A132-530[»]
4IQWX-ray2.60A132-530[»]
4QZ8X-ray2.70A132-530[»]
4QZ9X-ray2.05A132-530[»]
4QZAX-ray2.15A132-530[»]
4QZBX-ray2.15A132-530[»]
4QZCX-ray2.75A132-530[»]
4QZDX-ray2.70A132-530[»]
4QZEX-ray2.25A132-530[»]
4QZFX-ray2.60A132-530[»]
4QZGX-ray2.75A132-530[»]
4QZHX-ray2.60A132-530[»]
4QZIX-ray2.65A132-530[»]
5D46X-ray2.80A132-530[»]
5D49X-ray1.99A132-530[»]
5D4BX-ray2.66A/B132-530[»]
ProteinModelPortaliP09838
SMRiP09838
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09838

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 124BRCTPROSITE-ProRule annotationAdd BLAST98

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni151 – 530Mediates interaction with DNTTIP2By similarityAdd BLAST380
Regioni258 – 262Involved in DNA binding2 Publications5
Regioni333 – 338Deoxynucleoside triphosphate bindingCombined sourcesCurated6
Regioni342 – 345Deoxynucleoside triphosphate bindingCombined sourcesCurated4
Regioni449 – 450Deoxynucleoside triphosphate bindingCombined sourcesCurated2

Sequence similaritiesi

Belongs to the DNA polymerase type-X family.Curated

Phylogenomic databases

eggNOGiKOG2534 Eukaryota
COG1796 LUCA
GeneTreeiENSGT00530000063002
HOGENOMiHOG000263600
HOVERGENiHBG003670
InParanoidiP09838
KOiK00977
OMAiQYACQRR
OrthoDBiEOG091G073W
PhylomeDBiP09838
TreeFamiTF103012

Family and domain databases

CDDicd00027 BRCT, 1 hit
cd00141 NT_POLXc, 1 hit
Gene3Di1.10.150.110, 1 hit
3.30.210.10, 1 hit
3.40.50.10190, 1 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR002054 DNA-dir_DNA_pol_X
IPR019843 DNA_pol-X_BS
IPR010996 DNA_pol_b-like_N
IPR028207 DNA_pol_B_palm_palm
IPR018944 DNA_pol_lambd_fingers_domain
IPR027421 DNA_pol_lamdba_lyase_dom_sf
IPR037160 DNA_Pol_thumb_sf
IPR022312 DNA_pol_X
IPR029398 PolB_thumb
IPR027292 TdT
IPR001726 TdT/Mu
PfamiView protein in Pfam
PF00533 BRCT, 1 hit
PF14792 DNA_pol_B_palm, 1 hit
PF14791 DNA_pol_B_thumb, 1 hit
PF10391 DNA_pol_lambd_f, 1 hit
PF14716 HHH_8, 1 hit
PIRSFiPIRSF000817 DNA_NT, 1 hit
PIRSF501175 TDT, 1 hit
PRINTSiPR00869 DNAPOLX
PR00871 DNAPOLXTDT
SMARTiView protein in SMART
SM00292 BRCT, 1 hit
SM00483 POLXc, 1 hit
SUPFAMiSSF47802 SSF47802, 1 hit
SSF52113 SSF52113, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit
PS00522 DNA_POLYMERASE_X, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform TDT-L (identifier: P09838-1) [UniParc]FASTAAdd to basket
Also known as: TDT-Large, TdtL

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPLQAVHLG PRKKRPRQLG TPVASTPYDI RFRDLVLFIL EKKMGTTRRA
60 70 80 90 100
FLMELARRKG FRVENELSDS VTHIVAENNS GSDVLEWLQL QNIKASSELE
110 120 130 140 150
LLDISWLIEC MGAGKPVEMM GRHQLVVNRN SSPSPVPGSQ NVPAPAVKKI
160 170 180 190 200
SQYACQRRTT LNNYNQLFTD ALDILAENDE LRENEGSCLA FMRASSVLKS
210 220 230 240 250
LPFPITSMKD TEGIPCLGDK VKSIIEGIIE DGESSEAKAV LNDERYKSFK
260 270 280 290 300
LFTSVFGVGL KTAEKWFRMG FRTLSKIQSD KSLRFTQMQK AGFLYYEDLV
310 320 330 340 350
SCVNRPEAEA VSMLVKEAVV TFLPDALVTM TGGFRRGKMT GHDVDFLITS
360 370 380 390 400
PEATEDEEQQ LLHKVTDFWK QQGLLLYCDI LESTFEKFKQ PSRKVDALDH
410 420 430 440 450
FQKCFLILKL DHGRVHSEKS GQQEGKGWKA IRVDLVMCPY DRRAFALLGW
460 470 480 490 500
TGSRQFERDL RRYATHERKM MLDNHALYDR TKGKTVTISP LDGKVSKLQK
510 520 530
ALRVFLEAES EEEIFAHLGL DYIEPWERNA
Note: Inactivated at physiological temperature but is stable at lower temperatures.
Length:530
Mass (Da):60,331
Last modified:November 16, 2001 - v3
Checksum:iE6B109DCF39C8107
GO
Isoform TDT-S (identifier: P09838-2) [UniParc]FASTAAdd to basket
Also known as: TDT-Small, TdtS

The sequence of this isoform differs from the canonical sequence as follows:
     483-502: Missing.

Note: Major form.
Show »
Length:510
Mass (Da):58,266
Checksum:iCF6E850EE36EE3BF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26T → M in CAA48634 (PubMed:8464703).Curated1
Sequence conflicti99L → F in CAA48634 (PubMed:8464703).Curated1
Sequence conflicti193R → G in CAA27735 (PubMed:3755527).Curated1
Sequence conflicti287Q → K in CAA27735 (PubMed:3755527).Curated1
Sequence conflicti309E → Q in CAA27735 (PubMed:3755527).Curated1
Sequence conflicti367D → H in CAA27735 (PubMed:3755527).Curated1
Sequence conflicti441 – 445DRRAF → ECAC in CAA27735 (PubMed:3755527).Curated5

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001309483 – 502Missing in isoform TDT-S. 1 PublicationAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04123 mRNA Translation: CAA27735.1
X68670 mRNA Translation: CAA48634.2
AF316014 mRNA Translation: AAK07884.1
AF316015 mRNA Translation: AAK07885.1
AK087978 mRNA Translation: BAC40071.1
AK088709 mRNA Translation: BAC40518.1
CCDSiCCDS29807.1 [P09838-1]
CCDS37984.1 [P09838-2]
PIRiB23595
RefSeqiNP_001036693.1, NM_001043228.1 [P09838-2]
NP_033371.2, NM_009345.2 [P09838-1]
UniGeneiMm.25620

Genome annotation databases

EnsembliENSMUST00000051806; ENSMUSP00000062078; ENSMUSG00000025014 [P09838-1]
ENSMUST00000112200; ENSMUSP00000107819; ENSMUSG00000025014 [P09838-2]
GeneIDi21673
KEGGimmu:21673
UCSCiuc008hlo.1 mouse [P09838-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTDT_MOUSE
AccessioniPrimary (citable) accession number: P09838
Secondary accession number(s): Q99PD0, Q99PD1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 16, 2001
Last modified: April 25, 2018
This is version 179 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
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