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Protein

Signal transduction histidine-protein kinase/phosphatase UhpB

Gene

uhpB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the UhpABC signaling cascade that controls the expression of the hexose phosphate transporter UhpT. UhpB functions as a membrane-associated protein kinase that autophosphorylates in response to interaction with UhpC, and subsequently transfers its phosphate group to the response regulator UhpA (PubMed:3038843, PubMed:8349544, PubMed:11053370, PubMed:11739766). Can also dephosphorylate UhpA (PubMed:11053370, PubMed:11739766).4 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.2 Publications

Enzyme regulationi

Kinase activity is stimulated by UhpC.1 Publication

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphoprotein phosphatase activity Source: EcoCyc
  • phosphorelay sensor kinase activity Source: EcoCyc

GO - Biological processi

  • phosphorelay signal transduction system Source: EcoCyc
  • protein autophosphorylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Protein phosphatase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:UHPB-MONOMER.
ECOL316407:JW3643-MONOMER.
BRENDAi2.7.13.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal transduction histidine-protein kinase/phosphatase UhpBCurated (EC:2.7.13.32 Publications, EC:3.1.3.-1 Publication)
Gene namesi
Name:uhpB1 Publication
Ordered Locus Names:b3668, JW3643
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11052. uhpB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei8 – 2821HelicalSequence analysisAdd
BLAST
Transmembranei36 – 5621HelicalSequence analysisAdd
BLAST
Transmembranei80 – 10021HelicalSequence analysisAdd
BLAST
Transmembranei112 – 13221HelicalSequence analysisAdd
BLAST
Transmembranei140 – 16021HelicalSequence analysisAdd
BLAST
Transmembranei185 – 20521HelicalSequence analysisAdd
BLAST
Transmembranei213 – 23321HelicalSequence analysisAdd
BLAST
Transmembranei253 – 27321HelicalSequence analysisAdd
BLAST
Topological domaini274 – 500227Cytoplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi313 – 3131H → E: Lack of autokinase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 500500Signal transduction histidine-protein kinase/phosphatase UhpBPRO_0000074890Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei313 – 3131Phosphohistidine; by autocatalysis1 Publication

Post-translational modificationi

Autophosphorylated.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP09835.
PRIDEiP09835.

Interactioni

Protein-protein interaction databases

BioGridi4262583. 14 interactions.
DIPiDIP-11083N.
STRINGi511145.b3668.

Structurei

3D structure databases

ProteinModelPortaliP09835.
SMRiP09835. Positions 290-496.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini311 – 499189Histidine kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000280063.
InParanoidiP09835.
KOiK07675.
OMAiGEAAWNA.
OrthoDBiEOG6W9X4M.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR007895. MASE1.
IPR011712. Sig_transdc_His_kin_sub3_dim/P.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF07730. HisKA_3. 1 hit.
PF05231. MASE1. 1 hit.
[Graphical view]
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09835-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTLFSRLIT VIACFFIFSA AWFCLWSISL HLVERPDMAV LLFPFGLRLG
60 70 80 90 100
LMLQCPRGYW PVLLGAEWLL IYWLTQAVGL THFPLLMIGS LLTLLPVALI
110 120 130 140 150
SRYRHQRDWR TLLLQGAALT AAALLQSLPW LWHGKESWNA LLLTLTGGLT
160 170 180 190 200
LAPICLVFWH YLANNTWLPL GPSLVSQPIN WRGRHLVWYL LLFVISLWLQ
210 220 230 240 250
LGLPDELSRF TPFCLALPII ALAWHYGWQG ALIATLMNAI ALIASQTWRD
260 270 280 290 300
HPVDLLLSLL VQSLTGLLLG AGIQRLRELN QSLQKELARN QHLAERLLET
310 320 330 340 350
EESVRRDVAR ELHDDIGQTI TAIRTQAGIV QRLAADNASV KQSGQLIEQL
360 370 380 390 400
SLGVYDAVRR LLGRLRPRQL DDLTLEQAIR SLMREMELEG RGIVSHLEWR
410 420 430 440 450
IDESALSENQ RVTLFRVCQE GLNNIVKHAD ASAVTLQGWQ QDERLMLVIE
460 470 480 490 500
DDGSGLPPGS GQQGFGLTGM RERVTALGGT LHISCLHGTR VSVSLPQRYV
Length:500
Mass (Da):56,306
Last modified:August 1, 1992 - v3
Checksum:iA7353FC1F1D53152
GO

Sequence cautioni

The sequence AAA62020.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti337 – 35721NASVK…GVYDA → RRQREAERAAHRTTIAGRLR R in AAA24721 (PubMed:3301805).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17102 Genomic DNA. Translation: AAA24721.1.
M89479 Genomic DNA. Translation: AAA24725.1.
L10328 Genomic DNA. Translation: AAA62020.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76691.2.
AP009048 Genomic DNA. Translation: BAE77625.1.
PIRiE65168. RGECUB.
RefSeqiNP_418124.4. NC_000913.3.
WP_001295243.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76691; AAC76691; b3668.
BAE77625; BAE77625; BAE77625.
GeneIDi948195.
KEGGiecj:JW3643.
eco:b3668.
PATRICi32122829. VBIEscCol129921_3790.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17102 Genomic DNA. Translation: AAA24721.1.
M89479 Genomic DNA. Translation: AAA24725.1.
L10328 Genomic DNA. Translation: AAA62020.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76691.2.
AP009048 Genomic DNA. Translation: BAE77625.1.
PIRiE65168. RGECUB.
RefSeqiNP_418124.4. NC_000913.3.
WP_001295243.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP09835.
SMRiP09835. Positions 290-496.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262583. 14 interactions.
DIPiDIP-11083N.
STRINGi511145.b3668.

Proteomic databases

PaxDbiP09835.
PRIDEiP09835.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76691; AAC76691; b3668.
BAE77625; BAE77625; BAE77625.
GeneIDi948195.
KEGGiecj:JW3643.
eco:b3668.
PATRICi32122829. VBIEscCol129921_3790.

Organism-specific databases

EchoBASEiEB1045.
EcoGeneiEG11052. uhpB.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000280063.
InParanoidiP09835.
KOiK07675.
OMAiGEAAWNA.
OrthoDBiEOG6W9X4M.

Enzyme and pathway databases

BioCyciEcoCyc:UHPB-MONOMER.
ECOL316407:JW3643-MONOMER.
BRENDAi2.7.13.3. 2026.

Miscellaneous databases

PROiP09835.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR007895. MASE1.
IPR011712. Sig_transdc_His_kin_sub3_dim/P.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF07730. HisKA_3. 1 hit.
PF05231. MASE1. 1 hit.
[Graphical view]
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the uhp region of Escherichia coli."
    Friedrich M.J., Kadner R.J.
    J. Bacteriol. 169:3556-3563(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Kadner R.J.
    Submitted (AUG-1990) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "Structure and function of the uhp genes for the sugar phosphate transport system in Escherichia coli and Salmonella typhimurium."
    Island M.D., Wei B.-Y., Kadner R.J.
    J. Bacteriol. 174:2754-2762(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
    Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
    Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  6. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  7. "Identification of uhp polypeptides and evidence for their role in exogenous induction of the sugar phosphate transport system of Escherichia coli K-12."
    Weston L.A., Kadner R.J.
    J. Bacteriol. 169:3546-3555(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, FUNCTION.
  8. "Interplay between the membrane-associated UhpB and UhpC regulatory proteins."
    Island M.D., Kadner R.J.
    J. Bacteriol. 175:5028-5034(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "The histidine kinase domain of UhpB inhibits UhpA action at the Escherichia coli uhpT promoter."
    Wright J.S., Olekhnovich I.N., Touchie G., Kadner R.J.
    J. Bacteriol. 182:6279-6286(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, AUTOPHOSPHORYLATION, MUTAGENESIS OF HIS-313.
  10. "Glucose-6-phosphate-dependent phosphoryl flow through the Uhp two-component regulatory system."
    Verhamme D.T., Arents J.C., Postma P.W., Crielaard W., Hellingwerf K.J.
    Microbiology 147:3345-3352(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A KINASE AND A PHOSPHATASE, CATALYTIC ACTIVITY, AUTOPHOSPHORYLATION.
  11. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiUHPB_ECOLI
AccessioniPrimary (citable) accession number: P09835
Secondary accession number(s): P76729, Q2M7Y1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: August 1, 1992
Last modified: July 6, 2016
This is version 151 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.