Glutamate synthase [NADPH] large chain precursor

Sequence

>sp|P09831|GLTB_ECOLI Glutamate synthase [NADPH] large chain OS=Escherichia coli (strain K12) GN=gltB PE=1 SV=2 MTRKPRRHALSVPVRSGSEVGFPQSLGEVHDMLYDKSLERDNCGFGLIAHIEGEPSHKVV RTAIHALARMQHRGAILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLN KDPELAAAARRIVEEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWR PRDMERRLFIARRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPTDLPRFYLDLADLRLES AICLFHQRFSTNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDA APFVNETGSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMH MEPWDGPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKG RVGPGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSREL DDDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQQ FAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLTTMK EEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLPVPAPM AVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETLGRLVDTH AIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDVVGLCFQGAV SRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPDVVRTLQQAVQS GEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFKRFDTAAMSIGALS PEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGRFGVTPAYLVNADVIQ IKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHHDIYSIEDLAQLIFDLKQ VNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGTGASPLSSVKYAGCPWELGL VETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAESFGFGTGPMVALGCKYLRICH LNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARETRELMAQLGVTRLVDLIGRTDLL KELDGFTAKQQKLALSKLLETAEPHPGKALYCTENNPPFDNGLLNAQLLQQAKPFVDERQ SKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLAADPIKAYFNGTAGQSFGVWNAGGVELY LTGDANDYVGKGMAGGLIAIRPPVGSAFRSHEASIIGNTCLYGATGGRLYAAGRAGERFG VRNSGAITVVEGIGDNGCEYMTGGIVCILGKTGVNFGAGMTGGFAYVLDESGDFRKRVNP ELVEVLSVDALAIHEEHLRGLITEHVQHTGSQRGEEILANWSTFATKFALVKPKSSDVKA LLGHRSRSAAELRVQAQ

Options »

Blast tips

You can enter:
• a protein or nucleotide sequence
• a valid UniProt identifier, for example:
P00750 or A4_HUMAN or UPI0000000001
More...

Contribute

Send feedback

Read comments (1) or add your own

Reviewed, UniProtKB/Swiss-Prot P09831 (GLTB_ECOLI)

Last modified June 16, 2009. Version 94. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein names

Recommended name:
    Glutamate synthase [NADPH] large chain
    EC=1.4.1.13
Alternative name(s):
    Glutamate synthase subunit alpha
      Short name=GLTS alpha chain
    NADPH-GOGAT

Gene names

Name:	gltB
Synonyms:	aspB
Ordered Locus Names:	b3212, JW3179

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Hide | Top

Protein attributes

Sequence length	1517 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

Hide | Top

General annotation (Comments)

Catalytic activity	2 L-glutamate + NADP⁺ = L-glutamine + 2-oxoglutarate + NADPH.
Cofactor	Binds 1 3Fe-4S cluster. FAD. FMN.
Pathway	Amino-acid biosynthesis; L-glutamate biosynthesis via GLT pathway; L-glutamate from 2-oxoglutarate and L-glutamine (NADPH route): step 1/1. Energy metabolism; nitrogen metabolism.
Subunit structure	Aggregate of 4 catalytic active heterodimers, consisting of a large and a small subunit.
Miscellaneous	Glutamine binds to the large subunit and transfers the amido group to 2-oxo-glutamate that apparently binds to the small subunit.
Sequence similarities	Belongs to the glutamate synthase family. Contains 1 glutamine amidotransferase type-2 domain.

Hide | Top

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Glutamate biosynthesis
Domain	Glutamine amidotransferase
Ligand	3Fe-4S FAD FMN Flavoprotein Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
PTM	Zymogen
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate synthase (NADPH) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Hide | Top

Binary interactions

With	Entry	#Exp.	IntAct	Notes
gltD	P09832	2	EBI-551179,EBI-544293

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Propeptide

1 – 42

PRO_0000011620

Chain

43 – 1517

1475

Glutamate synthase [NADPH] large chain

PRO_0000011621

Regions

Domain

43 – 433

391

Glutamine amidotransferase type-2

Nucleotide binding

1080 – 1132

FMN By similarity

Sites

Active site

For GATase activity By similarity

Metal binding

1133

Iron-sulfur (3Fe-4S) By similarity

Metal binding

1139

Iron-sulfur (3Fe-4S) By similarity

Metal binding

1144

Iron-sulfur (3Fe-4S) By similarity

Experimental info

Sequence conflict

105 – 109

GWRLA → LAFR in AAA23904. Ref.1

Sequence conflict

140

E → N in AAA23904. Ref.1

Sequence conflict

220 – 240

LCMPT…LRLES → CVCRRICRVLSGSCGPASGM in AAA23904. Ref.1

Sequence conflict

259 – 282

LAQPF…NRQWA → WRNRSAIWRITVKSTPSPVT ANG Ref.1

Sequence conflict

259 – 282

LAQPF…NRQWA → WRNRSAIWRITVKSTPSPVT ANG Ref.4

Sequence conflict

460

N → K in AAA23904. Ref.1

Sequence conflict

574

A → T in AAA23904. Ref.1

Sequence conflict

863

D → H in AAA23904. Ref.1

Sequence conflict

1286 – 1290

THGDQ → DARRS in AAA23904. Ref.1

Sequence conflict

1373

G → A in AAA23904. Ref.1

Sequence conflict

1376

G → R in AAA23904. Ref.1

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

P09831-1 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 7524A143B5DD4F9A
Show »

FASTA

1,517

166,710

        10         20         30         40         50         60 
MTRKPRRHAL SVPVRSGSEV GFPQSLGEVH DMLYDKSLER DNCGFGLIAH IEGEPSHKVV 

        70         80         90        100        110        120 
RTAIHALARM QHRGAILADG KTGDGCGLLL QKPDRFFRIV AQERGWRLAK NYAVGMLFLN 

       130        140        150        160        170        180 
KDPELAAAAR RIVEEELQRE TLSIVGWRDV PTNEGVLGEI ALSSLPRIEQ IFVNAPAGWR 

       190        200        210        220        230        240 
PRDMERRLFI ARRRIEKRLE ADKDFYVCSL SNLVNIYKGL CMPTDLPRFY LDLADLRLES 

       250        260        270        280        290        300 
AICLFHQRFS TNTVPRWPLA QPFRYLAHNG EINTITGNRQ WARARTYKFQ TPLIPDLHDA 

       310        320        330        340        350        360 
APFVNETGSD SSSMDNMLEL LLAGGMDIIR AMRLLVPPAW QNNPDMDPEL RAFFDFNSMH 

       370        380        390        400        410        420 
MEPWDGPAGI VMSDGRFAAC NLDRNGLRPA RYVITKDKLI TCASEVGIWD YQPDEVVEKG 

       430        440        450        460        470        480 
RVGPGELMVI DTRSGRILHS AETDDDLKSR HPYKEWMEKN VRRLVPFEDL PDEEVGSREL 

       490        500        510        520        530        540 
DDDTLASYQK QFNYSAEELD SVIRVLGENG QEAVGSMGDD TPFAVLSSQP RIIYDYFRQQ 

       550        560        570        580        590        600 
FAQVTNPPID PLREAHVMSL ATSIGREMNV FCEAEGQAHR LSFKSPILLY SDFKQLTTMK 

       610        620        630        640        650        660 
EEHYRADTLD ITFDVTKTTL EATVKELCDK AEKMVRSGTV LLVLSDRNIA KDRLPVPAPM 

       670        680        690        700        710        720 
AVGAIQTRLV DQSLRCDANI IVETASARDP HHFAVLLGFG ATAIYPYLAY ETLGRLVDTH 

       730        740        750        760        770        780 
AIAKDYRTVM LNYRNGINKG LYKIMSKMGI STIASYRCSK LFEAVGLHDD VVGLCFQGAV 

       790        800        810        820        830        840 
SRIGGASFED FQQDLLNLSK RAWLARKPIS QGGLLKYVHG GEYHAYNPDV VRTLQQAVQS 

       850        860        870        880        890        900 
GEYSDYQEYA KLVNERPATT LRDLLAITPG ENAVNIADVE PASELFKRFD TAAMSIGALS 

       910        920        930        940        950        960 
PEAHEALAEA MNSIGGNSNS GEGGEDPARY GTNKVSRIKQ VASGRFGVTP AYLVNADVIQ 

       970        980        990       1000       1010       1020 
IKVAQGAKPG EGGQLPGDKV TPYIAKLRYS VPGVTLISPP PHHDIYSIED LAQLIFDLKQ 

      1030       1040       1050       1060       1070       1080 
VNPKAMISVK LVSEPGVGTI ATGVAKAYAD LITIAGYDGG TGASPLSSVK YAGCPWELGL 

      1090       1100       1110       1120       1130       1140 
VETQQALVAN GLRHKIRLQV DGGLKTGVDI IKAAILGAES FGFGTGPMVA LGCKYLRICH 

      1150       1160       1170       1180       1190       1200 
LNNCATGVAT QDDKLRKNHY HGLPFKVTNY FEFIARETRE LMAQLGVTRL VDLIGRTDLL 

      1210       1220       1230       1240       1250       1260 
KELDGFTAKQ QKLALSKLLE TAEPHPGKAL YCTENNPPFD NGLLNAQLLQ QAKPFVDERQ 

      1270       1280       1290       1300       1310       1320 
SKTFWFDIRN TDRSVGASLS GYIAQTHGDQ GLAADPIKAY FNGTAGQSFG VWNAGGVELY 

      1330       1340       1350       1360       1370       1380 
LTGDANDYVG KGMAGGLIAI RPPVGSAFRS HEASIIGNTC LYGATGGRLY AAGRAGERFG 

      1390       1400       1410       1420       1430       1440 
VRNSGAITVV EGIGDNGCEY MTGGIVCILG KTGVNFGAGM TGGFAYVLDE SGDFRKRVNP 

      1450       1460       1470       1480       1490       1500 
ELVEVLSVDA LAIHEEHLRG LITEHVQHTG SQRGEEILAN WSTFATKFAL VKPKSSDVKA 

      1510 
LLGHRSRSAA ELRVQAQ

« Hide

Hide | Top

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Determination of the nucleotide sequence for the glutamate synthase structural genes of Escherichia coli K-12." Oliver G., Gosset G., Sanchez-Pescador R., Lozoya E., Ku L.M., Flores N., Becerril B., Valle F., Bolivar F. Gene 60:1-11(1987) [PubMed: 3326786] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Mutations affecting the Shine-Dalgarno sequences of the untranslated region of the Escherichia coli gltBDF operon." Velazquez L., Camarena L., Reyes J.L., Bastarrachea F. J. Bacteriol. 173:3261-3264(1991) [PubMed: 1673677] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-304.
[5]	"Amino acid sequence analysis of the glutamate synthase enzyme from Escherichia coli K-12." Gosset G., Merino E., Recillas F., Oliver G., Becerril B., Bolivar F. Protein Seq. Data Anal. 2:9-16(1989) [PubMed: 2643092] [Abstract] Cited for: DISCUSSION OF SEQUENCE.

Hide | Top

Cross-references

Sequence databases
	M18747 Genomic DNA. Translation: AAA23904.1. U18997 Genomic DNA. Translation: AAA58014.1. U00096 Genomic DNA. Translation: AAC76244.1. AP009048 Genomic DNA. Translation: BAE77256.1. M68876 Genomic DNA. Translation: AAA23906.1.
PIR	F65112.
RefSeq	AP_003755.1. NP_417679.1.
3D structure databases
HSSP	HSSP built from PDB template 1LM1 based on UniProtKB P55038.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:9802N.
IntAct	P09831. 8 interactions.
Genome annotation databases
GeneID	947724.
GenomeReviews	Gene locus JW3179 in contig AP009048_GR. Gene locus b3212 in contig U00096_GR.
KEGG	ecj:JW3179. eco:b3212.
Organism-specific databases
EchoBASE	EB0398.
EcoGene	EG10403. gltB.
CMR	Search...
Phylogenomic databases
HOGENOM	P09831.
OMA	P09831. DSAAMSI.
Enzyme and pathway databases
BioCyc	EcoCyc:GLUSYNLARGE-MON. MetaCyc:GLUSYNLARGE-MON.
BRENDA	1.4.1.13. 246.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR000583. GATase_2. IPR017932. GATase_II. IPR002932. Glu_synth_centr_C. IPR006982. Glu_synth_centr_N. IPR002489. Glu_synthase_C. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 2 hits. G3DSA:2.160.20.60. Glu_synthase_C. 1 hit.
Pfam	PF00310. GATase_2. 1 hit. PF04898. Glu_syn_central. 1 hit. PF01645. Glu_synthase. 1 hit. PF01493. GXGXG. 1 hit. [Graphical view]
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Hide | Top

Entry information

Entry name

GLTB_ECOLI

Accession

Primary (citable) accession number: P09831
Secondary accession number(s): Q2M900

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents