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P09826

- GLNA1_RHILV

UniProt

P09826 - GLNA1_RHILV

Protein

Glutamine synthetase 1

Gene

glnA

Organism
Rhizobium leguminosarum bv. viciae
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    Enzyme regulationi

    The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive By similarity.By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro
    2. nitrogen fixation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Nitrogen fixation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciRETL1328306-WGS:GSTH-2110-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase 1 (EC:6.3.1.2)
    Alternative name(s):
    Glutamate--ammonia ligase I
    Glutamine synthetase I
    Short name:
    GSI
    Gene namesi
    Name:glnA
    OrganismiRhizobium leguminosarum bv. viciae
    Taxonomic identifieri387 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 469469Glutamine synthetase 1PRO_0000153223Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei398 – 3981O-AMP-tyrosineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    ProMEXiP09826.

    Interactioni

    Subunit structurei

    Oligomer of 12 subunits arranged in the form of two hexagons.

    Structurei

    3D structure databases

    ProteinModelPortaliP09826.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Family and domain databases

    Gene3Di3.10.20.70. 1 hit.
    3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR004809. Gln_synth_I.
    IPR001637. Gln_synth_I_adenylation_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    TIGRFAMsiTIGR00653. GlnA. 1 hit.
    PROSITEiPS00182. GLNA_ADENYLATION. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P09826-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATASEILKQ IKENDVKFVD LRFTDRRASL QHVTMDVVCV DEDMFADGVM    50
    FDGFSIGGWK AINESDMVLM PDTETVHMDP FFAQSTMVIV CDILDPVSGE 100
    AYNRDRRGTA KKAEAYLKAS GIGDTVFVGR EAEFFVFDDV KYKADPYNTG 150
    FKLDSTELPS NDDTDYETGN LGHRPRVKGG YFPVPPVDSA QDMRSEMLTV 200
    LSEMGVVVEK HHHEVAAAQH DTLVRNADEL GIKFKMQIYK YVVHQVANAY 250
    GKTATFMPKP IFGDNGSGMH VHQSIWKGGK PTFAGDEYAG LSESCLFYIG 300
    GIIKHAKAIN AFTNPSTNSY KRFVPGYEAP VLLAYSARNR SASCRIPFGS 350
    NPKAKRVEVR FPDPTANPYL AFAAMLMAGL DGIKNKIHPG KAMDKDLYDL 400
    PPKELKKIPT VCGSLRQALE SLDKDRKFLT AGGVFDDDQI DAFIELKMAE 450
    VMRFEMTPHP VEYDMYYSA 469
    Length:469
    Mass (Da):52,233
    Last modified:July 1, 1989 - v1
    Checksum:iA1E158ECD0970FBC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04880 Genomic DNA. Translation: CAA28569.1.
    PIRiA26567. AJZRQL.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04880 Genomic DNA. Translation: CAA28569.1 .
    PIRi A26567. AJZRQL.

    3D structure databases

    ProteinModelPortali P09826.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    ProMEXi P09826.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci RETL1328306-WGS:GSTH-2110-MONOMER.

    Family and domain databases

    Gene3Di 3.10.20.70. 1 hit.
    3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR004809. Gln_synth_I.
    IPR001637. Gln_synth_I_adenylation_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    TIGRFAMsi TIGR00653. GlnA. 1 hit.
    PROSITEi PS00182. GLNA_ADENYLATION. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Tight linkage of glnA and a putative regulatory gene in Rhizobium leguminosarum."
      Colonna-Romano S., Riccio A., Guida M., Defez R., Lamberti A., Iaccarino M., Arnold W., Priefer U., Puehler A.
      Nucleic Acids Res. 15:1951-1964(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: RCC1001.

    Entry informationi

    Entry nameiGLNA1_RHILV
    AccessioniPrimary (citable) accession number: P09826
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Two forms of glutamine synthetase (GSI and GSII) can be found in this nitrogen fixing bacteria, GSI is a typical prokaryotic glutamine synthetase whereas GSII is similar to the eukaryotic enzyme.

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3