P09826 (GLNA1_RHILV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamine synthetase 1
EC=6.3.1.2

Alternative name(s):
Glutamate--ammonia ligase I
Glutamine synthetase I
Short name=GSI

Gene names

Name:

glnA

Organism

Rhizobium leguminosarum bv. viciae

Taxonomic identifier

387 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.
Enzyme regulation	The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive By similarity.
Subunit structure	Oligomer of 12 subunits arranged in the form of two hexagons.
Subcellular location	Cytoplasm.
Miscellaneous	Two forms of glutamine synthetase (GSI and GSII) can be found in this nitrogen fixing bacteria, GSI is a typical prokaryotic glutamine synthetase whereas GSII is similar to the eukaryotic enzyme.
Sequence similarities	Belongs to the glutamine synthetase family.

Ontologies

Keywords
Biological process	Nitrogen fixation
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	glutamine biosynthetic process Inferred from electronic annotation. Source: InterPro nitrogen fixation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Glutamine synthetase 1		PRO_0000153223
Amino acid modifications
	Modified residue	398	1	O-AMP-tyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P09826 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: A1E158ECD0970FBC	FASTA	469	52,233
	10 20 30 40 50 60 MATASEILKQ IKENDVKFVD LRFTDRRASL QHVTMDVVCV DEDMFADGVM FDGFSIGGWK 70 80 90 100 110 120 AINESDMVLM PDTETVHMDP FFAQSTMVIV CDILDPVSGE AYNRDRRGTA KKAEAYLKAS 130 140 150 160 170 180 GIGDTVFVGR EAEFFVFDDV KYKADPYNTG FKLDSTELPS NDDTDYETGN LGHRPRVKGG 190 200 210 220 230 240 YFPVPPVDSA QDMRSEMLTV LSEMGVVVEK HHHEVAAAQH DTLVRNADEL GIKFKMQIYK 250 260 270 280 290 300 YVVHQVANAY GKTATFMPKP IFGDNGSGMH VHQSIWKGGK PTFAGDEYAG LSESCLFYIG 310 320 330 340 350 360 GIIKHAKAIN AFTNPSTNSY KRFVPGYEAP VLLAYSARNR SASCRIPFGS NPKAKRVEVR 370 380 390 400 410 420 FPDPTANPYL AFAAMLMAGL DGIKNKIHPG KAMDKDLYDL PPKELKKIPT VCGSLRQALE 430 440 450 460 SLDKDRKFLT AGGVFDDDQI DAFIELKMAE VMRFEMTPHP VEYDMYYSA

References

[1]	"Tight linkage of glnA and a putative regulatory gene in Rhizobium leguminosarum." Colonna-Romano S., Riccio A., Guida M., Defez R., Lamberti A., Iaccarino M., Arnold W., Priefer U., Puehler A. Nucleic Acids Res. 15:1951-1964(1987) [PubMed: 2882467] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RCC1001.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X04880 Genomic DNA. Translation: CAA28569.1.
PIR	AJZRQL. A26567.
3D structure databases
ProteinModelPortal	P09826.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008147. Gln_synt_beta. IPR014746. Gln_synth/guanido_kin_cat_dom. IPR008146. Gln_synth_cat_dom. IPR004809. Gln_synth_I. IPR001637. Gln_synth_I_adenylation_site. [Graphical view]
Gene3D	G3DSA:3.30.590.10. ATP-gua_Ptrans. 1 hit. G3DSA:3.10.20.70. G3DSA:3.10.20.70. 1 hit.
Pfam	PF00120. Gln-synt_C. 1 hit. PF03951. Gln-synt_N. 1 hit. [Graphical view]
SUPFAM	SSF54368. Gln_synt_beta. 1 hit.
TIGRFAMs	TIGR00653. GlnA. 1 hit.
PROSITE	PS00180. GLNA_1. False negative. PS00182. GLNA_ADENYLATION. 1 hit. PS00181. GLNA_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLNA1_RHILV

Accession

Primary (citable) accession number: P09826

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	May 31, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program