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P09813

- APOA2_MOUSE

UniProt

P09813 - APOA2_MOUSE

Protein

Apolipoprotein A-II

Gene

Apoa2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.

    GO - Molecular functioni

    1. cholesterol binding Source: Ensembl
    2. cholesterol transporter activity Source: Ensembl
    3. high-density lipoprotein particle binding Source: MGI
    4. lipase inhibitor activity Source: Ensembl
    5. phosphatidylcholine binding Source: Ensembl
    6. phosphatidylcholine-sterol O-acyltransferase activator activity Source: Ensembl
    7. protein heterodimerization activity Source: UniProtKB

    GO - Biological processi

    1. acute inflammatory response Source: Ensembl
    2. beta-glucoside transport Source: UniProtKB
    3. cholesterol efflux Source: Ensembl
    4. cholesterol homeostasis Source: MGI
    5. cholesterol metabolic process Source: MGI
    6. diacylglycerol catabolic process Source: Ensembl
    7. fatty acid metabolic process Source: UniProtKB
    8. high-density lipoprotein particle assembly Source: Ensembl
    9. high-density lipoprotein particle clearance Source: Ensembl
    10. high-density lipoprotein particle remodeling Source: Ensembl
    11. lipid transport Source: MGI
    12. lipoprotein metabolic process Source: MGI
    13. low-density lipoprotein particle remodeling Source: Ensembl
    14. negative regulation of cholesterol import Source: Ensembl
    15. negative regulation of cholesterol transporter activity Source: Ensembl
    16. negative regulation of cytokine secretion involved in immune response Source: Ensembl
    17. negative regulation of lipase activity Source: MGI
    18. negative regulation of lipid catabolic process Source: Ensembl
    19. negative regulation of very-low-density lipoprotein particle remodeling Source: Ensembl
    20. organ regeneration Source: Ensembl
    21. peptidyl-methionine modification Source: Ensembl
    22. phosphatidylcholine biosynthetic process Source: Ensembl
    23. phospholipid catabolic process Source: Ensembl
    24. phospholipid efflux Source: Ensembl
    25. positive regulation of cholesterol esterification Source: Ensembl
    26. positive regulation of interleukin-8 biosynthetic process Source: UniProtKB
    27. positive regulation of lipid catabolic process Source: Ensembl
    28. protein folding Source: Ensembl
    29. protein oxidation Source: Ensembl
    30. regulation of intestinal cholesterol absorption Source: MGI
    31. regulation of protein stability Source: Ensembl
    32. response to drug Source: Ensembl
    33. response to estrogen Source: Ensembl
    34. response to glucocorticoid Source: Ensembl
    35. response to glucose Source: Ensembl
    36. reverse cholesterol transport Source: Ensembl
    37. triglyceride-rich lipoprotein particle remodeling Source: Ensembl

    Keywords - Biological processi

    Lipid transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_198569. Retinoid metabolism and transport.
    REACT_198602. PPARA activates gene expression.
    REACT_216017. Chylomicron-mediated lipid transport.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Apolipoprotein A-II
    Short name:
    Apo-AII
    Short name:
    ApoA-II
    Alternative name(s):
    Apolipoprotein A2
    Cleaved into the following chain:
    Proapolipoprotein A-II
    Short name:
    ProapoA-II
    Gene namesi
    Name:Apoa2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:88050. Apoa2.

    Subcellular locationi

    GO - Cellular componenti

    1. chylomicron Source: Ensembl
    2. cytosol Source: Reactome
    3. extracellular region Source: Reactome
    4. extracellular space Source: MGI
    5. spherical high-density lipoprotein particle Source: Ensembl
    6. very-low-density lipoprotein particle Source: Ensembl

    Keywords - Cellular componenti

    Amyloid, HDL, Secreted

    Pathology & Biotechi

    Involvement in diseasei

    Defects in Apoa2 are the cause of senescence accelerated mouse (SAM), the senile amyloid is a mutated apolipoprotein A-II.

    Keywords - Diseasei

    Disease mutation

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Add
    BLAST
    Chaini19 – 10284Proapolipoprotein A-IIPRO_0000425354Add
    BLAST
    Chaini24 – 10279Apolipoprotein A-IIPRO_0000002008Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei24 – 241Pyrrolidone carboxylic acid; in Apolipoprotein A-IIBy similarity
    Modified residuei49 – 491Methionine sulfoxideBy similarity

    Post-translational modificationi

    Phosphorylation sites are present in the extracellular medium.By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Oxidation, Phosphoprotein, Pyrrolidone carboxylic acid

    Proteomic databases

    MaxQBiP09813.
    PaxDbiP09813.
    PRIDEiP09813.

    PTM databases

    PhosphoSiteiP09813.

    Expressioni

    Tissue specificityi

    Plasma.

    Gene expression databases

    CleanExiMM_APOA2.
    GenevestigatoriP09813.

    Interactioni

    Subunit structurei

    Monomer. Interacts with APOA1BP and NDRG1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi198156. 1 interaction.
    IntActiP09813. 5 interactions.
    MINTiMINT-1855567.
    STRINGi10090.ENSMUSP00000106951.

    Structurei

    3D structure databases

    ProteinModelPortaliP09813.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the apolipoprotein A2 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG40617.
    GeneTreeiENSGT00390000003306.
    HOGENOMiHOG000033999.
    HOVERGENiHBG050544.
    InParanoidiP09813.
    KOiK08758.
    OMAiAYFEKTQ.
    OrthoDBiEOG7G4QHG.
    TreeFamiTF338165.

    Family and domain databases

    InterProiIPR006801. ApoA-II.
    [Graphical view]
    PANTHERiPTHR11027. PTHR11027. 1 hit.
    PfamiPF04711. ApoA-II. 1 hit.
    [Graphical view]
    ProDomiPD010397. ApoA-II. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09813-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLLAMVALL VTICSLEGAL VKRQADGPDM QSLFTQYFQS MTDYGKDLME    50
    KAKTSEIQSQ AKAYFEKTHE QLTPLVRSAG TSLVNFFSSL MNLEEKPAPA 100
    AK 102
    Length:102
    Mass (Da):11,309
    Last modified:October 3, 2012 - v2
    Checksum:iF50142E0D118665B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti43 – 431D → E in CAA27731. (PubMed:2426658)Curated
    Sequence conflicti43 – 431D → E in AAA37248. (PubMed:2514123)Curated
    Sequence conflicti43 – 431D → E in AAA37250. (PubMed:2118905)Curated
    Sequence conflicti43 – 431D → E in AAH31786. (PubMed:15489334)Curated
    Sequence conflicti48 – 481L → F in CAA44616. (PubMed:1683229)Curated
    Sequence conflicti49 – 491M → V in CAA27731. (PubMed:2426658)Curated
    Sequence conflicti49 – 491M → V in AAA37248. (PubMed:2514123)Curated
    Sequence conflicti49 – 491M → V in AAA37250. (PubMed:2118905)Curated
    Sequence conflicti49 – 491M → V in AAH31786. (PubMed:15489334)Curated
    Sequence conflicti61 – 611A → V in CAA27731. (PubMed:2426658)Curated
    Sequence conflicti61 – 611A → V in AAA37250. (PubMed:2118905)Curated
    Sequence conflicti61 – 611A → V in AAH31786. (PubMed:15489334)Curated

    Mass spectrometryi

    Molecular mass is 8709.2±0.071 Da from positions 24 - 102. Determined by ESI. Strain C57BL/6. Without methionine sulfoxide.1 Publication
    Molecular mass is 8719.5 Da from positions 24 - 102. Determined by ESI. Strain BALB/c. Without methionine sulfoxide.1 Publication
    Molecular mass is 8725.3±0.283 Da from positions 24 - 102. Determined by ESI. Strain C57BL/6. With 1 methionine sulfoxide.1 Publication
    Molecular mass is 8742 Da from positions 24 - 102. Determined by ESI. Strain C57BL/6. With 2 methionine sulfoxides.1 Publication
    Molecular mass is 8735.2 Da from positions 24 - 102. Determined by ESI. Strain BALB/c. With 1 methionine sulfoxide.1 Publication
    Molecular mass is 9294±0.707 Da from positions 19 - 102. Determined by ESI. Strain C57BL/6. Without methionine sulfoxide.1 Publication
    Molecular mass is 9304 Da from positions 19 - 102. Determined by ESI. Strain BALB/c. Without methionine sulfoxide.1 Publication

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti28 – 281P → Q in SAM. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04119 mRNA. Translation: CAA27731.1.
    M32360 Genomic DNA. Translation: AAA37248.1.
    M79361 mRNA. Translation: AAA37249.1.
    M79362 mRNA. Translation: AAA37250.1.
    X62772 mRNA. Translation: CAA44616.1.
    AK145823 mRNA. Translation: BAE26675.1.
    AC084821 Genomic DNA. No translation available.
    CH466520 Genomic DNA. Translation: EDL39118.1.
    CH466520 Genomic DNA. Translation: EDL39119.1.
    CH466520 Genomic DNA. Translation: EDL39120.1.
    CH466520 Genomic DNA. Translation: EDL39121.1.
    BC031786 mRNA. Translation: AAH31786.1.
    CCDSiCCDS35773.1.
    PIRiA37887.
    B37887. B23594.
    I48250.
    JS0392. A23594.
    RefSeqiNP_038502.2. NM_013474.2.
    XP_006496688.1. XM_006496625.1.
    UniGeneiMm.389209.

    Genome annotation databases

    EnsembliENSMUST00000005824; ENSMUSP00000005824; ENSMUSG00000005681.
    ENSMUST00000111319; ENSMUSP00000106951; ENSMUSG00000005681.
    ENSMUST00000111320; ENSMUSP00000106952; ENSMUSG00000005681.
    ENSMUST00000111321; ENSMUSP00000106953; ENSMUSG00000005681.
    GeneIDi11807.
    KEGGimmu:11807.
    UCSCiuc007dnk.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04119 mRNA. Translation: CAA27731.1 .
    M32360 Genomic DNA. Translation: AAA37248.1 .
    M79361 mRNA. Translation: AAA37249.1 .
    M79362 mRNA. Translation: AAA37250.1 .
    X62772 mRNA. Translation: CAA44616.1 .
    AK145823 mRNA. Translation: BAE26675.1 .
    AC084821 Genomic DNA. No translation available.
    CH466520 Genomic DNA. Translation: EDL39118.1 .
    CH466520 Genomic DNA. Translation: EDL39119.1 .
    CH466520 Genomic DNA. Translation: EDL39120.1 .
    CH466520 Genomic DNA. Translation: EDL39121.1 .
    BC031786 mRNA. Translation: AAH31786.1 .
    CCDSi CCDS35773.1.
    PIRi A37887.
    B37887. B23594.
    I48250.
    JS0392. A23594.
    RefSeqi NP_038502.2. NM_013474.2.
    XP_006496688.1. XM_006496625.1.
    UniGenei Mm.389209.

    3D structure databases

    ProteinModelPortali P09813.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198156. 1 interaction.
    IntActi P09813. 5 interactions.
    MINTi MINT-1855567.
    STRINGi 10090.ENSMUSP00000106951.

    PTM databases

    PhosphoSitei P09813.

    Proteomic databases

    MaxQBi P09813.
    PaxDbi P09813.
    PRIDEi P09813.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000005824 ; ENSMUSP00000005824 ; ENSMUSG00000005681 .
    ENSMUST00000111319 ; ENSMUSP00000106951 ; ENSMUSG00000005681 .
    ENSMUST00000111320 ; ENSMUSP00000106952 ; ENSMUSG00000005681 .
    ENSMUST00000111321 ; ENSMUSP00000106953 ; ENSMUSG00000005681 .
    GeneIDi 11807.
    KEGGi mmu:11807.
    UCSCi uc007dnk.2. mouse.

    Organism-specific databases

    CTDi 336.
    MGIi MGI:88050. Apoa2.

    Phylogenomic databases

    eggNOGi NOG40617.
    GeneTreei ENSGT00390000003306.
    HOGENOMi HOG000033999.
    HOVERGENi HBG050544.
    InParanoidi P09813.
    KOi K08758.
    OMAi AYFEKTQ.
    OrthoDBi EOG7G4QHG.
    TreeFami TF338165.

    Enzyme and pathway databases

    Reactomei REACT_198569. Retinoid metabolism and transport.
    REACT_198602. PPARA activates gene expression.
    REACT_216017. Chylomicron-mediated lipid transport.

    Miscellaneous databases

    ChiTaRSi APOA2. mouse.
    NextBioi 279671.
    PROi P09813.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_APOA2.
    Genevestigatori P09813.

    Family and domain databases

    InterProi IPR006801. ApoA-II.
    [Graphical view ]
    PANTHERi PTHR11027. PTHR11027. 1 hit.
    Pfami PF04711. ApoA-II. 1 hit.
    [Graphical view ]
    ProDomi PD010397. ApoA-II. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and nucleotide sequence of cDNA for murine senile amyloid protein: nucleotide substitutions found in apolipoprotein A-II cDNA of senescence accelerated mouse (SAM)."
      Kunisada T., Higuchi K., Aota S., Takeda T., Yamagishi H.
      Nucleic Acids Res. 14:5729-5740(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Structural organization of the gene encoding apolipoprotein A-II in an amyloidotic strain of senescence-accelerated mouse."
      Yonezu T., Toda M., Yamagishi H., Higuchi K., Takeda T.
      Gene 84:187-191(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT SAM GLN-28.
    3. "A polymorphism affecting apolipoprotein A-II translational efficiency determines high density lipoprotein size and composition."
      Doolittle M.H., Leboeuf R.C., Warden C.H., Bee L.M., Lusis A.J.
      J. Biol. Chem. 265:16380-16388(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/cJ, C3H/HeJ, C57BL/6J and DBA/2J.
      Tissue: Liver.
    4. "Polymorphism of apolipoprotein A-II (apoA-II) among inbred strains of mice. Relationship between the molecular type of apoA-II and mouse senile amyloidosis."
      Higuchi K., Kitagawa K., Naiki H., Hanada K., Hosakawa M., Takeda T.
      Biochem. J. 279:427-433(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6J.
    5. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Amnion.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    7. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver.
    9. "The single proline-glutamine substitution at position 5 enhances the potency of amyloid fibril formation of murine apo A-II."
      Higuchi K., Yonezu T., Tsunasawa S., Sakiyama F., Takeda T.
      FEBS Lett. 207:23-27(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 24-102.
    10. "High homology is present in the primary structures between murine senile amyloid protein (ASSAM) and human apolipoprotein A-II."
      Yonezu T., Higuchi K., Tsunasawa S., Takagi S., Sakiyama F., Takeda T.
      FEBS Lett. 203:149-152(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 24-102.
    11. "Mass spectral analysis of the apolipoproteins on mouse high density lipoproteins. Detection of post-translational modifications."
      Puppione D.L., Yam L.M., Bassilian S., Souda P., Castellani L.W., Schumaker V.N., Whitelegge J.P.
      Biochim. Biophys. Acta 1764:1363-1371(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 54-62 AND 68-77, MASS SPECTROMETRY, OXIDATION.

    Entry informationi

    Entry nameiAPOA2_MOUSE
    AccessioniPrimary (citable) accession number: P09813
    Secondary accession number(s): Q3UKX6, Q61317
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 137 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The apo A-II stoichiometry in HDL molecules varies among inbred mice strains, because of structural polymorphisms affecting the apo A-II gene, which influence its translational efficiency.
    The sequence presented here is that of strain BALB/c and C3H/HeJ.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3