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Protein

Glycogen phosphorylase, liver form

Gene

Pygl

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.

Catalytic activityi

((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate.

Cofactori

Enzyme regulationi

Activity of phosphorylase is controlled both by allosteric means (through the noncovalent binding of metabolites) and by covalent modification. Thus AMP allosterically activates, whereas ATP, ADP, and glucose-6-phosphate allosterically inhibit, phosphorylase B.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei76AMPBy similarity1
Sitei109Involved in the association of subunitsBy similarity1
Sitei143Involved in the association of subunitsBy similarity1
Sitei156May be involved in allosteric controlBy similarity1

GO - Molecular functioni

GO - Biological processi

  • 5-phosphoribose 1-diphosphate biosynthetic process Source: RGD
  • glucose homeostasis Source: Ensembl
  • glycogen catabolic process Source: RGD
  • glycogen metabolic process Source: RGD
  • necroptotic process Source: Ensembl

Keywordsi

Molecular functionAllosteric enzyme, Glycosyltransferase, Transferase
Biological processCarbohydrate metabolism, Glycogen metabolism
LigandNucleotide-binding, Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-RNO-6798695 Neutrophil degranulation
R-RNO-70221 Glycogen breakdown (glycogenolysis)

Protein family/group databases

CAZyiGT35 Glycosyltransferase Family 35

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen phosphorylase, liver form (EC:2.4.1.1)
Gene namesi
Name:Pygl
Synonyms:Lgp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi620687 Pygl

Subcellular locationi

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3239

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001885262 – 850Glycogen phosphorylase, liver formAdd BLAST849

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei15Phosphoserine; by PHK; in form phosphorylase ABy similarity1
Modified residuei364N6-succinyllysineBy similarity1
Modified residuei524PhosphoserineBy similarity1
Modified residuei561PhosphoserineCombined sources1
Modified residuei639PhosphoserineCombined sources1
Modified residuei681N6-(pyridoxal phosphate)lysineBy similarity1

Post-translational modificationi

Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP09811
PRIDEiP09811

PTM databases

iPTMnetiP09811
PhosphoSitePlusiP09811

Expressioni

Gene expression databases

BgeeiENSRNOG00000006388
GenevisibleiP09811 RN

Interactioni

Subunit structurei

Homodimer. Dimers associate into a tetramer to form the enzymatically active phosphorylase A. Interacts with PPP1R3B.3 Publications

GO - Molecular functioni

  • protein homodimerization activity Source: RGD

Protein-protein interaction databases

IntActiP09811, 1 interactor
STRINGi10116.ENSRNOP00000009183

Chemistry databases

BindingDBiP09811

Structurei

3D structure databases

ProteinModelPortaliP09811
SMRiP09811
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycogen phosphorylase family.Curated

Phylogenomic databases

eggNOGiKOG2099 Eukaryota
COG0058 LUCA
GeneTreeiENSGT00390000016886
HOGENOMiHOG000278444
HOVERGENiHBG006848
InParanoidiP09811
KOiK00688
OMAiARDIWNM
OrthoDBiEOG091G03RB
PhylomeDBiP09811
TreeFamiTF300309

Family and domain databases

CDDicd04300 GT1_Glycogen_Phosphorylase, 1 hit
InterProiView protein in InterPro
IPR011833 Glycg_phsphrylas
IPR000811 Glyco_trans_35
IPR035090 Pyridoxal_P_attach_site
PANTHERiPTHR11468 PTHR11468, 1 hit
PfamiView protein in Pfam
PF00343 Phosphorylase, 1 hit
PIRSFiPIRSF000460 Pprylas_GlgP, 1 hit
TIGRFAMsiTIGR02093 P_ylase, 1 hit
PROSITEiView protein in PROSITE
PS00102 PHOSPHORYLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09811-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKPLTDQEK RRQISIRGIV GVENVAELKK GFNRHLHFTL VKDRNVATPR
60 70 80 90 100
DYYFALAHTV RDHLVGRWIR TQQHYYDKCP KRVYYLSLEF YMGRTLQNTM
110 120 130 140 150
INLGLQNACD EAIYQLGLDM EELEEIEEDA GLGNGGLGRL AACFLDSMAT
160 170 180 190 200
LGLAAYGYGI RYEYGIFNQK IREGWQVEEA DDWLRHGNPW EKARPEFMLP
210 220 230 240 250
VHFYGRVEHT QAGTKWVDTQ VVLALPYDTP VPGYMNNTVN TMRLWSARAP
260 270 280 290 300
NDFNLQDFNV GDYIQAVLDR NLAENISRVL YPNDNFFEGK ELRLKQEYFV
310 320 330 340 350
VAATLQDVIR RFKASKFGSK DGVGTVFDAF PDQVAIQLND THPALAIPEL
360 370 380 390 400
MRIFVDIEKL PWSKAWEITK KTFAYTNHTV LPEALERWPV DLVEKLLPRH
410 420 430 440 450
LQIIYEINQK HLDRIVALFP KDIDRMRRMS LIEEEGGKRI NMAHLCIVGC
460 470 480 490 500
HAVNGVAKIH SDIVKTQVFK DFSELEPDKF QNKTNGITPR RWLLLCNPGL
510 520 530 540 550
ADLIAEKIGE DYVKDLSQLT KLHSFVGDDI FLREIAKVKQ ENKLKFSQFL
560 570 580 590 600
EKEYKVKINP SSMFDVHVKR IHEYKRQLLN CLHVITMYNR IKKDPKKFFV
610 620 630 640 650
PRTVIIGGKA APGYHMAKMI IKLVTSVAEV VNNDPMVGSK LKVIFLENYR
660 670 680 690 700
VSLAEKVIPA TDLSEQISTA GTEASGTGNM KFMLNGALTI GTMDGANVEM
710 720 730 740 750
AEEAGEENLF IFGMRVDDVA ALDKKGYEAK EYYEALPELK LVIDQIDNGF
760 770 780 790 800
FSPNQPDLFK DIINMLFYHD RFKVFADYEA YVKCQEKVSQ LYMNQKAWNT
810 820 830 840 850
MVLRNIAASG KFSSDRTIRE YAKDIWNMEP SDLKISLSKE SSNGVNANGK
Length:850
Mass (Da):97,483
Last modified:January 23, 2007 - v5
Checksum:i0BE9A641DFFF3E0D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti667I → V in CAA27704 (PubMed:2424788).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63515 mRNA Translation: CAA45083.1
BC070901 mRNA Translation: AAH70901.1
M85280 Genomic DNA Translation: AAA41254.1
J03080 mRNA Translation: AAA41986.1
X04069 mRNA Translation: CAA27704.1
M59460 mRNA Translation: AAA41987.1
PIRiS22338
RefSeqiNP_071604.1, NM_022268.1
UniGeneiRn.21399

Genome annotation databases

EnsembliENSRNOT00000009183; ENSRNOP00000009183; ENSRNOG00000006388
GeneIDi64035
KEGGirno:64035
UCSCiRGD:620687 rat

Similar proteinsi

Entry informationi

Entry nameiPYGL_RAT
AccessioniPrimary (citable) accession number: P09811
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 167 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health