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P09810

- TPH1_RAT

UniProt

P09810 - TPH1_RAT

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Protein
Tryptophan 5-hydroxylase 1
Gene
Tph1, Tph
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.

Cofactori

Fe2+ ion.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei235 – 2351Tryptophan By similarity
Binding sitei257 – 2571Tryptophan By similarity
Binding sitei265 – 2651Tryptophan By similarity
Metal bindingi272 – 2721Iron By similarity
Metal bindingi277 – 2771Iron By similarity
Metal bindingi317 – 3171Iron By similarity
Binding sitei336 – 3361Tryptophan By similarity
Binding sitei366 – 3661Tryptophan; via carbonyl oxygen By similarity

GO - Molecular functioni

  1. amino acid binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. tryptophan 5-monooxygenase activity Source: RGD

GO - Biological processi

  1. bone remodeling Source: Ensembl
  2. circadian rhythm Source: RGD
  3. mammary gland alveolus development Source: Ensembl
  4. negative regulation of ossification Source: RGD
  5. response to immobilization stress Source: RGD
  6. serotonin biosynthetic process from tryptophan Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Serotonin biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

SABIO-RKP09810.
UniPathwayiUPA00846; UER00799.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan 5-hydroxylase 1 (EC:1.14.16.4)
Alternative name(s):
Tryptophan 5-monooxygenase 1
Gene namesi
Name:Tph1
Synonyms:Tph
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi3895. Tph1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: RGD
  2. neuron projection Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444Tryptophan 5-hydroxylase 1
PRO_0000205571Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei58 – 581Phosphoserine; by PKA Reviewed prediction

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP09810.
PRIDEiP09810.

PTM databases

PhosphoSiteiP09810.

Expressioni

Gene expression databases

GenevestigatoriP09810.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000052959.

Structurei

3D structure databases

ProteinModelPortaliP09810.
SMRiP09810. Positions 104-439.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 9476ACT
Add
BLAST

Sequence similaritiesi

Contains 1 ACT domain.

Phylogenomic databases

eggNOGiCOG3186.
GeneTreeiENSGT00390000010268.
HOGENOMiHOG000233373.
HOVERGENiHBG006841.
InParanoidiP09810.
KOiK00502.
OMAiTWGTVFQ.
OrthoDBiEOG7KM5T1.
PhylomeDBiP09810.
TreeFamiTF313327.

Family and domain databases

Gene3Di1.10.800.10. 1 hit.
InterProiIPR002912. ACT_dom.
IPR001273. ArAA_hydroxylase.
IPR018301. ArAA_hydroxylase_Fe/CU_BS.
IPR019774. Aromatic-AA_hydroxylase_C.
IPR005963. Trp_5_mOase.
IPR019773. Tyrosine_3-monooxygenase-like.
[Graphical view]
PANTHERiPTHR11473. PTHR11473. 1 hit.
PfamiPF01842. ACT. 1 hit.
PF00351. Biopterin_H. 1 hit.
[Graphical view]
PIRSFiPIRSF000336. TH. 1 hit.
PRINTSiPR00372. FYWHYDRXLASE.
SUPFAMiSSF56534. SSF56534. 1 hit.
TIGRFAMsiTIGR01270. Trp_5_monoox. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
PS00367. BH4_AAA_HYDROXYL_1. 1 hit.
PS51410. BH4_AAA_HYDROXYL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09810-1 [UniParc]FASTAAdd to Basket

« Hide

MIEDNKENKD HSSERGRVTL IFSLKNEVGG LIKALKIFQE NHVNLLHIES    50
RKSKRRNSEF EIFVDCDINR EQLNDIFPLL KSHTTVLSVD SPDQLPEKED 100
VMETVPWFPK KISDLDFCAN RVLLYGSELD ADHPGFKDNV YRRRRKYFAE 150
LAMNYKHGDP IPKIEFTEEE IKTWGTIFRE LNKLYPTHAC REYLRNLPLL 200
SKYCGYREDN VPQLEDVSNF LKERTGFSIR PVAGYLSPRD FLSGLAFRVF 250
HCTQYVRHSS DPLYTPEPDT CHELLGHVPL LAEPSFAQFS QEIGLASLGA 300
SEETVQKLAT CYFFTVEFGL CKQDGQLRVF GAGLLSSISE LRHALSGHAK 350
VKPFDPKVAC KQECLITSFQ DVYFVSESFE DAKEKMREFA KTVKRPFGVK 400
YNPYTQSIQV LRDSKSITSA MNELRHDLDV VNDALARVSR WPSV 444
Length:444
Mass (Da):51,068
Last modified:October 1, 1989 - v2
Checksum:iC3CF5245727CC825
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M28000 mRNA. Translation: AAA42262.1.
X53501 mRNA. Translation: CAA37579.1.
PIRiJL0034. WHRTW.
RefSeqiNP_001094104.1. NM_001100634.2.
XP_006229281.1. XM_006229219.1.
XP_006229282.1. XM_006229220.1.
UniGeneiRn.94788.

Genome annotation databases

EnsembliENSRNOT00000056109; ENSRNOP00000052959; ENSRNOG00000011672.
GeneIDi24848.
KEGGirno:24848.
UCSCiRGD:3895. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M28000 mRNA. Translation: AAA42262.1 .
X53501 mRNA. Translation: CAA37579.1 .
PIRi JL0034. WHRTW.
RefSeqi NP_001094104.1. NM_001100634.2.
XP_006229281.1. XM_006229219.1.
XP_006229282.1. XM_006229220.1.
UniGenei Rn.94788.

3D structure databases

ProteinModelPortali P09810.
SMRi P09810. Positions 104-439.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000052959.

Chemistry

BindingDBi P09810.
ChEMBLi CHEMBL4809.

PTM databases

PhosphoSitei P09810.

Proteomic databases

PaxDbi P09810.
PRIDEi P09810.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000056109 ; ENSRNOP00000052959 ; ENSRNOG00000011672 .
GeneIDi 24848.
KEGGi rno:24848.
UCSCi RGD:3895. rat.

Organism-specific databases

CTDi 7166.
RGDi 3895. Tph1.

Phylogenomic databases

eggNOGi COG3186.
GeneTreei ENSGT00390000010268.
HOGENOMi HOG000233373.
HOVERGENi HBG006841.
InParanoidi P09810.
KOi K00502.
OMAi TWGTVFQ.
OrthoDBi EOG7KM5T1.
PhylomeDBi P09810.
TreeFami TF313327.

Enzyme and pathway databases

UniPathwayi UPA00846 ; UER00799 .
SABIO-RK P09810.

Miscellaneous databases

NextBioi 604610.
PROi P09810.

Gene expression databases

Genevestigatori P09810.

Family and domain databases

Gene3Di 1.10.800.10. 1 hit.
InterProi IPR002912. ACT_dom.
IPR001273. ArAA_hydroxylase.
IPR018301. ArAA_hydroxylase_Fe/CU_BS.
IPR019774. Aromatic-AA_hydroxylase_C.
IPR005963. Trp_5_mOase.
IPR019773. Tyrosine_3-monooxygenase-like.
[Graphical view ]
PANTHERi PTHR11473. PTHR11473. 1 hit.
Pfami PF01842. ACT. 1 hit.
PF00351. Biopterin_H. 1 hit.
[Graphical view ]
PIRSFi PIRSF000336. TH. 1 hit.
PRINTSi PR00372. FYWHYDRXLASE.
SUPFAMi SSF56534. SSF56534. 1 hit.
TIGRFAMsi TIGR01270. Trp_5_monoox. 1 hit.
PROSITEi PS51671. ACT. 1 hit.
PS00367. BH4_AAA_HYDROXYL_1. 1 hit.
PS51410. BH4_AAA_HYDROXYL_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of two mRNAs encoding active rat tryptophan hydroxylase."
    Darmon M.C., Guibert B., Leviel V., Ehret M., Maitre M., Mallet J.
    J. Neurochem. 51:312-316(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Pineal gland.
  2. "Molecular cloning and characterization of cDNA encoding tryptophan hydroxylase from rat central serotonergic neurons."
    Kim K.S., Wessel T.C., Stone D.M., Carver C.H., Joh T.H., Park D.H.
    Brain Res. Mol. Brain Res. 9:277-283(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Isolation of a rat pineal gland cDNA clone homologous to tyrosine and phenylalanine hydroxylases."
    Darmon M.C., Grima B., Cash C.D., Maitre M., Mallet J.
    FEBS Lett. 206:43-46(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 167-261.

Entry informationi

Entry nameiTPH1_RAT
AccessioniPrimary (citable) accession number: P09810
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 1, 1989
Last modified: April 16, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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