P09810 (TPH1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan 5-hydroxylase 1
EC=1.14.16.4

Alternative name(s):
Tryptophan 5-monooxygenase 1

Gene names

Name:	Tph1
Synonyms:	Tph

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.
Cofactor	Fe2+ ion.
Pathway	Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Serotonin biosynthesis
Ligand	Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	response to stress Inferred from expression pattern. Source: RGD serotonin biosynthetic process from tryptophan Traceable author statement. Source: RGD
Cellular component	cytoplasm Traceable author statement. Source: RGD neuron projection Inferred from direct assay. Source: RGD
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro tryptophan 5-monooxygenase activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Tryptophan 5-hydroxylase 1		PRO_0000205571
Regions
	Domain	18 – 92	75	ACT
Sites
	Metal binding	272	1	Iron By similarity
	Metal binding	277	1	Iron By similarity
	Metal binding	317	1	Iron By similarity
	Binding site	235	1	Tryptophan By similarity
	Binding site	257	1	Tryptophan By similarity
	Binding site	265	1	Tryptophan By similarity
	Binding site	336	1	Tryptophan By similarity
	Binding site	366	1	Tryptophan; via carbonyl oxygen By similarity
Amino acid modifications
	Modified residue	58	1	Phosphoserine; by PKA Potential

Sequences

	Sequence		Length	Mass (Da)
	P09810 [UniParc]. Last modified October 1, 1989. Version 2. Checksum: C3CF5245727CC825	FASTA	444	51,068
	10 20 30 40 50 60 MIEDNKENKD HSSERGRVTL IFSLKNEVGG LIKALKIFQE NHVNLLHIES RKSKRRNSEF 70 80 90 100 110 120 EIFVDCDINR EQLNDIFPLL KSHTTVLSVD SPDQLPEKED VMETVPWFPK KISDLDFCAN 130 140 150 160 170 180 RVLLYGSELD ADHPGFKDNV YRRRRKYFAE LAMNYKHGDP IPKIEFTEEE IKTWGTIFRE 190 200 210 220 230 240 LNKLYPTHAC REYLRNLPLL SKYCGYREDN VPQLEDVSNF LKERTGFSIR PVAGYLSPRD 250 260 270 280 290 300 FLSGLAFRVF HCTQYVRHSS DPLYTPEPDT CHELLGHVPL LAEPSFAQFS QEIGLASLGA 310 320 330 340 350 360 SEETVQKLAT CYFFTVEFGL CKQDGQLRVF GAGLLSSISE LRHALSGHAK VKPFDPKVAC 370 380 390 400 410 420 KQECLITSFQ DVYFVSESFE DAKEKMREFA KTVKRPFGVK YNPYTQSIQV LRDSKSITSA 430 440 MNELRHDLDV VNDALARVSR WPSV

References

[1]	"Sequence of two mRNAs encoding active rat tryptophan hydroxylase." Darmon M.C., Guibert B., Leviel V., Ehret M., Maitre M., Mallet J. J. Neurochem. 51:312-316(1988) [PubMed: 3379411] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Pineal gland.
[2]	"Molecular cloning and characterization of cDNA encoding tryptophan hydroxylase from rat central serotonergic neurons." Kim K.S., Wessel T.C., Stone D.M., Carver C.H., Joh T.H., Park D.H. Brain Res. Mol. Brain Res. 9:277-283(1991) [PubMed: 1645430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Isolation of a rat pineal gland cDNA clone homologous to tyrosine and phenylalanine hydroxylases." Darmon M.C., Grima B., Cash C.D., Maitre M., Mallet J. FEBS Lett. 206:43-46(1986) [PubMed: 2875901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 167-261.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M28000 mRNA. Translation: AAA42262.1. X53501 mRNA. Translation: CAA37579.1.
IPI	IPI00876589.
PIR	WHRTW. JL0034.
RefSeq	NP_001094104.1. NM_001100634.2.
UniGene	Rn.94788.
3D structure databases
ProteinModelPortal	P09810.
SMR	P09810. Positions 104-439.
ModBase	Search...
Protein-protein interaction databases
STRING	P09810.
PTM databases
PhosphoSite	P09810.
Proteomic databases
PRIDE	P09810.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000056109; ENSRNOP00000052959; ENSRNOG00000011672.
GeneID	24848.
KEGG	rno:24848.
Organism-specific databases
CTD	7166.
RGD	3895. Tph1.
Phylogenomic databases
eggNOG	maNOG10549.
GeneTree	ENSGT00390000010268.
HOVERGEN	HBG006841.
InParanoid	P09810.
OMA	TWGTVFQ.
OrthoDB	EOG4S7JPZ.
PhylomeDB	P09810.
Gene expression databases
ArrayExpress	P09810.
Genevestigator	P09810.
GermOnline	ENSRNOG00000011672. Rattus norvegicus.
Family and domain databases
InterPro	IPR002912. ACT-bd. IPR001273. ArAA_hydroxylase. IPR018301. ArAA_hydroxylase_Fe/CU_BS. IPR019774. Aromatic-AA_hydroxylase_C. IPR005963. Trp_5_mOase. IPR019773. Tyrosine_3-monooxygenase-like. [Graphical view]
Gene3D	G3DSA:1.10.800.10. Aaa_hydroxylase. 1 hit.
KO	K00502.
PANTHER	PTHR11473. Aaa_hydroxylase. 1 hit.
Pfam	PF01842. ACT. 1 hit. PF00351. Biopterin_H. 1 hit. [Graphical view]
PIRSF	PIRSF000336. TH. 1 hit.
PRINTS	PR00372. FYWHYDRXLASE.
SUPFAM	SSF56534. Aaa_hydroxylase. 1 hit.
TIGRFAMs	TIGR01270. Trp_5_monoox. 1 hit.
PROSITE	PS00367. BH4_AAA_HYDROXYL_1. 1 hit. PS51410. BH4_AAA_HYDROXYL_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	604610.

Entry information

Entry name

TPH1_RAT

Accession

Primary (citable) accession number: P09810

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	October 1, 1989
Last modified:	November 16, 2011
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program