Reviewed, UniProtKB/Swiss-Prot P09810 (TPH1_RAT)

Names and origin

Protein names

Recommended name:
    Tryptophan 5-hydroxylase 1
    EC=1.14.16.4
Alternative name(s):
    Tryptophan 5-monooxygenase 1

Gene names

Name:	Tph1
Synonyms:	Tph

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.
Cofactor	Fe2+ ion.
Pathway	Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
Subunit structure	Multimer of identical subunits.
Sequence similarities	Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Serotonin biosynthesis
Ligand	Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from expression pattern. Source: RGD serotonin biosynthetic process from tryptophan Traceable author statement. Source: RGD
Cellular component	cytoplasm Traceable author statement. Source: RGD neuron projection Inferred from direct assay. Source: RGD
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW tryptophan 5-monooxygenase activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Tryptophan 5-hydroxylase 1		PRO_0000205571
Regions
	Domain	18 – 92	75	ACT
Sites
	Metal binding	272	1	Iron By similarity
	Metal binding	277	1	Iron By similarity
	Metal binding	317	1	Iron By similarity
Amino acid modifications
	Modified residue	58	1	Phosphoserine; by PKA Potential

Sequences

	Sequence		Length	Mass (Da)
	P09810-1 [UniParc]. Last modified October 1, 1989. Version 2. Checksum: C3CF5245727CC825	FASTA	444	51,068
	10 20 30 40 50 60 MIEDNKENKD HSSERGRVTL IFSLKNEVGG LIKALKIFQE NHVNLLHIES RKSKRRNSEF 70 80 90 100 110 120 EIFVDCDINR EQLNDIFPLL KSHTTVLSVD SPDQLPEKED VMETVPWFPK KISDLDFCAN 130 140 150 160 170 180 RVLLYGSELD ADHPGFKDNV YRRRRKYFAE LAMNYKHGDP IPKIEFTEEE IKTWGTIFRE 190 200 210 220 230 240 LNKLYPTHAC REYLRNLPLL SKYCGYREDN VPQLEDVSNF LKERTGFSIR PVAGYLSPRD 250 260 270 280 290 300 FLSGLAFRVF HCTQYVRHSS DPLYTPEPDT CHELLGHVPL LAEPSFAQFS QEIGLASLGA 310 320 330 340 350 360 SEETVQKLAT CYFFTVEFGL CKQDGQLRVF GAGLLSSISE LRHALSGHAK VKPFDPKVAC 370 380 390 400 410 420 KQECLITSFQ DVYFVSESFE DAKEKMREFA KTVKRPFGVK YNPYTQSIQV LRDSKSITSA 430 440 MNELRHDLDV VNDALARVSR WPSV

References

[1]	"Sequence of two mRNAs encoding active rat tryptophan hydroxylase." Darmon M.C., Guibert B., Leviel V., Ehret M., Maitre M., Mallet J. J. Neurochem. 51:312-316(1988) [PubMed: 3379411] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Pineal gland.
[2]	"Molecular cloning and characterization of cDNA encoding tryptophan hydroxylase from rat central serotonergic neurons." Kim K.S., Wessel T.C., Stone D.M., Carver C.H., Joh T.H., Park D.H. Brain Res. Mol. Brain Res. 9:277-283(1991) [PubMed: 1645430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Isolation of a rat pineal gland cDNA clone homologous to tyrosine and phenylalanine hydroxylases." Darmon M.C., Grima B., Cash C.D., Maitre M., Mallet J. FEBS Lett. 206:43-46(1986) [PubMed: 2875901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 167-261.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M28000 mRNA. Translation: AAA42262.1. X53501 mRNA. Translation: CAA37579.1.
IPI	IPI00876589.
PIR	WHRTW. JL0034.
RefSeq	NP_001094104.1.
UniGene	Rn.219628
3D structure databases
HSSP	HSSP built from PDB template 1MLW based on UniProtKB P17752.
SMR	P09810. Positions 104-393.
ModBase	Search...
PTM databases
PhosphoSite	P09810.
Proteomic databases
PRIDE	P09810.
Genome annotation databases
Ensembl	ENSRNOG00000011672. Rattus norvegicus. [Contig view]
GeneID	24848.
KEGG	rno:24848.
Organism-specific databases
RGD	3895. Tph1.
Phylogenomic databases
HOVERGEN	P09810.
OMA	P09810. TWGTVFQ.
Enzyme and pathway databases
BRENDA	1.14.16.4. 248.
Gene expression databases
ArrayExpress	P09810.
GermOnline	ENSRNOG00000011672. Rattus norvegicus.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR001273. ArAA_hydroxylase. IPR018301. ArAA_hydroxylase_Fe/CU_BS. IPR019774. Aromatic-AA_hydroxylase_C. IPR005963. Trp_5_mOase. IPR019773. Tyrosine_3-monooxygenase-like. [Graphical view]
Gene3D	G3DSA:1.10.800.10. Aaa_hydroxylase. 1 hit.
PANTHER	PTHR11473. Aaa_hydroxylase. 1 hit.
Pfam	PF01842. ACT. 1 hit. PF00351. Biopterin_H. 1 hit. [Graphical view]
PIRSF	PIRSF000336. TH. 1 hit.
PRINTS	PR00372. FYWHYDRXLASE.
ProDom	PD002559. Aaa_hydroxylase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01270. Trp_5_monoox. 1 hit.
PROSITE	PS00367. BIOPTERIN_HYDROXYL. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	604610.

Entry information

Entry name

TPH1_RAT

Accession

Primary (citable) accession number: P09810

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	October 1, 1989
Last modified:	June 16, 2009
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)