Tryptophan 5-hydroxylase 1 - Rattus norvegicus (Rat)

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P09810 (TPH1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 119. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Tryptophan 5-hydroxylase 1
EC=1.14.16.4

Alternative name(s):
Tryptophan 5-monooxygenase 1

Gene names

Name:	Tph1
Synonyms:	Tph

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	L-tryptophan + tetrahydrobiopterin + O₂ = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.
Cofactor	Fe²⁺ ion.
Pathway	Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Serotonin biosynthesis
Ligand	Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	bone remodeling Inferred from electronic annotation. Source: Compara circadian rhythm Inferred from expression pattern PubMed 18705647. Source: RGD mammary gland alveolus development Inferred from electronic annotation. Source: Compara negative regulation of ossification Inferred from mutant phenotype PubMed 20139991. Source: RGD response to immobilization stress Inferred from expression pattern PubMed 14744469. Source: RGD serotonin biosynthetic process from tryptophan Traceable author statement PubMed 12153488. Source: RGD
Cellular_component	cytoplasm Traceable author statement PubMed 12354109. Source: RGD neuron projection Inferred from direct assay PubMed 14744469. Source: RGD
Molecular_function	amino acid binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro tryptophan 5-monooxygenase activity Inferred from direct assay PubMed 12354109. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 444

444

Tryptophan 5-hydroxylase 1

PRO_0000205571

Regions

Domain

18 – 92

ACT

Sites

Metal binding

272

Iron By similarity

Metal binding

277

Iron By similarity

Metal binding

317

Iron By similarity

Binding site

235

Tryptophan By similarity

Binding site

257

Tryptophan By similarity

Binding site

265

Tryptophan By similarity

Binding site

336

Tryptophan By similarity

Binding site

366

Tryptophan; via carbonyl oxygen By similarity

Amino acid modifications

Modified residue

Phosphoserine; by PKA Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P09810 [UniParc].

Last modified October 1, 1989. Version 2.
Checksum: C3CF5245727CC825
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FASTA

444

51,068

        10         20         30         40         50         60 
MIEDNKENKD HSSERGRVTL IFSLKNEVGG LIKALKIFQE NHVNLLHIES RKSKRRNSEF 

        70         80         90        100        110        120 
EIFVDCDINR EQLNDIFPLL KSHTTVLSVD SPDQLPEKED VMETVPWFPK KISDLDFCAN 

       130        140        150        160        170        180 
RVLLYGSELD ADHPGFKDNV YRRRRKYFAE LAMNYKHGDP IPKIEFTEEE IKTWGTIFRE 

       190        200        210        220        230        240 
LNKLYPTHAC REYLRNLPLL SKYCGYREDN VPQLEDVSNF LKERTGFSIR PVAGYLSPRD 

       250        260        270        280        290        300 
FLSGLAFRVF HCTQYVRHSS DPLYTPEPDT CHELLGHVPL LAEPSFAQFS QEIGLASLGA 

       310        320        330        340        350        360 
SEETVQKLAT CYFFTVEFGL CKQDGQLRVF GAGLLSSISE LRHALSGHAK VKPFDPKVAC 

       370        380        390        400        410        420 
KQECLITSFQ DVYFVSESFE DAKEKMREFA KTVKRPFGVK YNPYTQSIQV LRDSKSITSA 

       430        440 
MNELRHDLDV VNDALARVSR WPSV

« Hide

References

[1]	"Sequence of two mRNAs encoding active rat tryptophan hydroxylase." Darmon M.C., Guibert B., Leviel V., Ehret M., Maitre M., Mallet J. J. Neurochem. 51:312-316(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Pineal gland.
[2]	"Molecular cloning and characterization of cDNA encoding tryptophan hydroxylase from rat central serotonergic neurons." Kim K.S., Wessel T.C., Stone D.M., Carver C.H., Joh T.H., Park D.H. Brain Res. Mol. Brain Res. 9:277-283(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Isolation of a rat pineal gland cDNA clone homologous to tyrosine and phenylalanine hydroxylases." Darmon M.C., Grima B., Cash C.D., Maitre M., Mallet J. FEBS Lett. 206:43-46(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 167-261.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M28000 mRNA. Translation: AAA42262.1. X53501 mRNA. Translation: CAA37579.1.
IPI	IPI00876589.
PIR	WHRTW. JL0034.
RefSeq	NP_001094104.1. NM_001100634.2.
UniGene	Rn.94788.
3D structure databases
ProteinModelPortal	P09810.
SMR	P09810. Positions 104-439.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000052959.
PTM databases
PhosphoSite	P09810.
Proteomic databases
PaxDb	P09810.
PRIDE	P09810.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000056109; ENSRNOP00000052959; ENSRNOG00000011672.
GeneID	24848.
KEGG	rno:24848.
UCSC	RGD:3895. rat.
Organism-specific databases
CTD	7166.
RGD	3895. Tph1.
Phylogenomic databases
eggNOG	COG3186.
GeneTree	ENSGT00390000010268.
HOGENOM	HOG000233373.
HOVERGEN	HBG006841.
InParanoid	P09810.
KO	K00502.
OMA	TWGTVFQ.
OrthoDB	EOG4S7JPZ.
Enzyme and pathway databases
SABIO-RK	P09810.
UniPathway	UPA00846; UER00799.
Gene expression databases
Genevestigator	P09810.
GermOnline	ENSRNOG00000011672. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.800.10. 1 hit.
InterPro	IPR002912. ACT_dom. IPR001273. ArAA_hydroxylase. IPR018301. ArAA_hydroxylase_Fe/CU_BS. IPR019774. Aromatic-AA_hydroxylase_C. IPR005963. Trp_5_mOase. IPR019773. Tyrosine_3-monooxygenase-like. [Graphical view]
PANTHER	PTHR11473. PTHR11473. 1 hit.
Pfam	PF01842. ACT. 1 hit. PF00351. Biopterin_H. 1 hit. [Graphical view]
PIRSF	PIRSF000336. TH. 1 hit.
PRINTS	PR00372. FYWHYDRXLASE.
SUPFAM	SSF56534. Aaa_hydroxylase. 1 hit.
TIGRFAMs	TIGR01270. Trp_5_monoox. 1 hit.
PROSITE	PS00367. BH4_AAA_HYDROXYL_1. 1 hit. PS51410. BH4_AAA_HYDROXYL_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P09810.
ChEMBL	CHEMBL4809.
NextBio	604610.

Entry information

Entry name

TPH1_RAT

Accession

Primary (citable) accession number: P09810

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	October 1, 1989
Last modified:	May 1, 2013
This is version 119 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents