Reviewed,
UniProtKB/Swiss-Prot P09810 (TPH1_RAT)
Last modified
June 16, 2009.
Version 85.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Tryptophan 5-hydroxylase 1 EC=1.14.16.4 Alternative name(s): Tryptophan 5-monooxygenase 1 | ||||
| Gene names |
| ||||
| Organism | Rattus norvegicus (Rat) | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 444 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Catalytic activity | L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin. |
| Cofactor | Fe2+ ion. |
| Pathway | Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2. |
| Subunit structure | Multimer of identical subunits. |
| Sequence similarities | Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Contains 1 ACT domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Serotonin biosynthesis |
| Ligand | Iron Metal-binding |
| Molecular function | Monooxygenase Oxidoreductase |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to stressInferred from expression pattern. Source: RGD serotonin biosynthetic process from tryptophanTraceable author statement. Source: RGD |
| Cellular component | cytoplasm Traceable author statement. Source: RGD neuron projectionInferred from direct assay. Source: RGD |
| Molecular function | amino acid binding Inferred from electronic annotation. Source: InterPro iron ion bindingInferred from electronic annotation. Source: UniProtKB-KW tryptophan 5-monooxygenase activityInferred from direct assay. Source: RGD |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 444 | 444 | Tryptophan 5-hydroxylase 1 | PRO_0000205571 | |||||
Regions | |||||||||
| Domain | 18 – 92 | 75 | ACT | ||||||
Sites | |||||||||
| Metal binding | 272 | 1 | Iron By similarity | ||||||
| Metal binding | 277 | 1 | Iron By similarity | ||||||
| Metal binding | 317 | 1 | Iron By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 58 | 1 | Phosphoserine; by PKA Potential | ||||||
Sequences
| ||||||||||||||||||
References
| [1] | "Sequence of two mRNAs encoding active rat tryptophan hydroxylase." Darmon M.C., Guibert B., Leviel V., Ehret M., Maitre M., Mallet J. J. Neurochem. 51:312-316(1988) [PubMed: 3379411] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Pineal gland. |
| [2] | "Molecular cloning and characterization of cDNA encoding tryptophan hydroxylase from rat central serotonergic neurons." Kim K.S., Wessel T.C., Stone D.M., Carver C.H., Joh T.H., Park D.H. Brain Res. Mol. Brain Res. 9:277-283(1991) [PubMed: 1645430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "Isolation of a rat pineal gland cDNA clone homologous to tyrosine and phenylalanine hydroxylases." Darmon M.C., Grima B., Cash C.D., Maitre M., Mallet J. FEBS Lett. 206:43-46(1986) [PubMed: 2875901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 167-261. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| M28000 mRNA. Translation: AAA42262.1. X53501 mRNA. Translation: CAA37579.1. | |
| IPI | IPI00876589. |
| PIR | WHRTW. JL0034. |
| RefSeq | NP_001094104.1. |
| UniGene | Rn.219628 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1MLW based on UniProtKB P17752. |
| SMR | P09810. Positions 104-393. |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | P09810. |
Proteomic databases | |
| PRIDE | P09810. |
Genome annotation databases | |
| Ensembl | ENSRNOG00000011672. Rattus norvegicus. [Contig view] |
| GeneID | 24848. |
| KEGG | rno:24848. |
Organism-specific databases | |
| RGD | 3895. Tph1. |
Phylogenomic databases | |
| HOVERGEN | P09810. |
| OMA | P09810. TWGTVFQ. |
Enzyme and pathway databases | |
| BRENDA | 1.14.16.4. 248. |
Gene expression databases | |
| ArrayExpress | P09810. |
| GermOnline | ENSRNOG00000011672. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR002912. ACT_bd. IPR001273. ArAA_hydroxylase. IPR018301. ArAA_hydroxylase_Fe/CU_BS. IPR019774. Aromatic-AA_hydroxylase_C. IPR005963. Trp_5_mOase. IPR019773. Tyrosine_3-monooxygenase-like. [Graphical view] |
| Gene3D | G3DSA:1.10.800.10. Aaa_hydroxylase. 1 hit. |
| PANTHER | PTHR11473. Aaa_hydroxylase. 1 hit. |
| Pfam | PF01842. ACT. 1 hit. PF00351. Biopterin_H. 1 hit. [Graphical view] |
| PIRSF | PIRSF000336. TH. 1 hit. |
| PRINTS | PR00372. FYWHYDRXLASE. |
| ProDom | PD002559. Aaa_hydroxylase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| TIGRFAMs | TIGR01270. Trp_5_monoox. 1 hit. |
| PROSITE | PS00367. BIOPTERIN_HYDROXYL. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 604610. |
Entry information
| Entry name | TPH1_RAT | ||||||||
| Accession | Primary (citable) accession number: P09810 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


