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P09810

- TPH1_RAT

UniProt

P09810 - TPH1_RAT

Protein

Tryptophan 5-hydroxylase 1

Gene

Tph1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 2 (01 Oct 1989)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.

    Cofactori

    Fe2+ ion.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei235 – 2351TryptophanBy similarity
    Binding sitei257 – 2571TryptophanBy similarity
    Binding sitei265 – 2651TryptophanBy similarity
    Metal bindingi272 – 2721IronBy similarity
    Metal bindingi277 – 2771IronBy similarity
    Metal bindingi317 – 3171IronBy similarity
    Binding sitei336 – 3361TryptophanBy similarity
    Binding sitei366 – 3661Tryptophan; via carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. amino acid binding Source: InterPro
    2. iron ion binding Source: InterPro
    3. tryptophan 5-monooxygenase activity Source: RGD

    GO - Biological processi

    1. bone remodeling Source: Ensembl
    2. circadian rhythm Source: RGD
    3. mammary gland alveolus development Source: Ensembl
    4. negative regulation of ossification Source: RGD
    5. response to immobilization stress Source: RGD
    6. serotonin biosynthetic process from tryptophan Source: RGD

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Serotonin biosynthesis

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    SABIO-RKP09810.
    UniPathwayiUPA00846; UER00799.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tryptophan 5-hydroxylase 1 (EC:1.14.16.4)
    Alternative name(s):
    Tryptophan 5-monooxygenase 1
    Gene namesi
    Name:Tph1
    Synonyms:Tph
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi3895. Tph1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: RGD
    2. neuron projection Source: RGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 444444Tryptophan 5-hydroxylase 1PRO_0000205571Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei58 – 581Phosphoserine; by PKASequence Analysis

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP09810.
    PRIDEiP09810.

    PTM databases

    PhosphoSiteiP09810.

    Expressioni

    Gene expression databases

    GenevestigatoriP09810.

    Interactioni

    Subunit structurei

    Homotetramer.

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000052959.

    Structurei

    3D structure databases

    ProteinModelPortaliP09810.
    SMRiP09810. Positions 104-439.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini19 – 9476ACTPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 ACT domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG3186.
    GeneTreeiENSGT00390000010268.
    HOGENOMiHOG000233373.
    HOVERGENiHBG006841.
    InParanoidiP09810.
    KOiK00502.
    OMAiTWGTVFQ.
    OrthoDBiEOG7KM5T1.
    PhylomeDBiP09810.
    TreeFamiTF313327.

    Family and domain databases

    Gene3Di1.10.800.10. 1 hit.
    InterProiIPR002912. ACT_dom.
    IPR001273. ArAA_hydroxylase.
    IPR018301. ArAA_hydroxylase_Fe/CU_BS.
    IPR019774. Aromatic-AA_hydroxylase_C.
    IPR005963. Trp_5_mOase.
    IPR019773. Tyrosine_3-monooxygenase-like.
    [Graphical view]
    PANTHERiPTHR11473. PTHR11473. 1 hit.
    PfamiPF01842. ACT. 1 hit.
    PF00351. Biopterin_H. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000336. TH. 1 hit.
    PRINTSiPR00372. FYWHYDRXLASE.
    SUPFAMiSSF56534. SSF56534. 1 hit.
    TIGRFAMsiTIGR01270. Trp_5_monoox. 1 hit.
    PROSITEiPS51671. ACT. 1 hit.
    PS00367. BH4_AAA_HYDROXYL_1. 1 hit.
    PS51410. BH4_AAA_HYDROXYL_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P09810-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIEDNKENKD HSSERGRVTL IFSLKNEVGG LIKALKIFQE NHVNLLHIES    50
    RKSKRRNSEF EIFVDCDINR EQLNDIFPLL KSHTTVLSVD SPDQLPEKED 100
    VMETVPWFPK KISDLDFCAN RVLLYGSELD ADHPGFKDNV YRRRRKYFAE 150
    LAMNYKHGDP IPKIEFTEEE IKTWGTIFRE LNKLYPTHAC REYLRNLPLL 200
    SKYCGYREDN VPQLEDVSNF LKERTGFSIR PVAGYLSPRD FLSGLAFRVF 250
    HCTQYVRHSS DPLYTPEPDT CHELLGHVPL LAEPSFAQFS QEIGLASLGA 300
    SEETVQKLAT CYFFTVEFGL CKQDGQLRVF GAGLLSSISE LRHALSGHAK 350
    VKPFDPKVAC KQECLITSFQ DVYFVSESFE DAKEKMREFA KTVKRPFGVK 400
    YNPYTQSIQV LRDSKSITSA MNELRHDLDV VNDALARVSR WPSV 444
    Length:444
    Mass (Da):51,068
    Last modified:October 1, 1989 - v2
    Checksum:iC3CF5245727CC825
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M28000 mRNA. Translation: AAA42262.1.
    X53501 mRNA. Translation: CAA37579.1.
    PIRiJL0034. WHRTW.
    RefSeqiNP_001094104.1. NM_001100634.2.
    XP_006229281.1. XM_006229219.1.
    XP_006229282.1. XM_006229220.1.
    UniGeneiRn.94788.

    Genome annotation databases

    EnsembliENSRNOT00000056109; ENSRNOP00000052959; ENSRNOG00000011672.
    GeneIDi24848.
    KEGGirno:24848.
    UCSCiRGD:3895. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M28000 mRNA. Translation: AAA42262.1 .
    X53501 mRNA. Translation: CAA37579.1 .
    PIRi JL0034. WHRTW.
    RefSeqi NP_001094104.1. NM_001100634.2.
    XP_006229281.1. XM_006229219.1.
    XP_006229282.1. XM_006229220.1.
    UniGenei Rn.94788.

    3D structure databases

    ProteinModelPortali P09810.
    SMRi P09810. Positions 104-439.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000052959.

    Chemistry

    BindingDBi P09810.
    ChEMBLi CHEMBL4809.

    PTM databases

    PhosphoSitei P09810.

    Proteomic databases

    PaxDbi P09810.
    PRIDEi P09810.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000056109 ; ENSRNOP00000052959 ; ENSRNOG00000011672 .
    GeneIDi 24848.
    KEGGi rno:24848.
    UCSCi RGD:3895. rat.

    Organism-specific databases

    CTDi 7166.
    RGDi 3895. Tph1.

    Phylogenomic databases

    eggNOGi COG3186.
    GeneTreei ENSGT00390000010268.
    HOGENOMi HOG000233373.
    HOVERGENi HBG006841.
    InParanoidi P09810.
    KOi K00502.
    OMAi TWGTVFQ.
    OrthoDBi EOG7KM5T1.
    PhylomeDBi P09810.
    TreeFami TF313327.

    Enzyme and pathway databases

    UniPathwayi UPA00846 ; UER00799 .
    SABIO-RK P09810.

    Miscellaneous databases

    NextBioi 604610.
    PROi P09810.

    Gene expression databases

    Genevestigatori P09810.

    Family and domain databases

    Gene3Di 1.10.800.10. 1 hit.
    InterProi IPR002912. ACT_dom.
    IPR001273. ArAA_hydroxylase.
    IPR018301. ArAA_hydroxylase_Fe/CU_BS.
    IPR019774. Aromatic-AA_hydroxylase_C.
    IPR005963. Trp_5_mOase.
    IPR019773. Tyrosine_3-monooxygenase-like.
    [Graphical view ]
    PANTHERi PTHR11473. PTHR11473. 1 hit.
    Pfami PF01842. ACT. 1 hit.
    PF00351. Biopterin_H. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000336. TH. 1 hit.
    PRINTSi PR00372. FYWHYDRXLASE.
    SUPFAMi SSF56534. SSF56534. 1 hit.
    TIGRFAMsi TIGR01270. Trp_5_monoox. 1 hit.
    PROSITEi PS51671. ACT. 1 hit.
    PS00367. BH4_AAA_HYDROXYL_1. 1 hit.
    PS51410. BH4_AAA_HYDROXYL_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of two mRNAs encoding active rat tryptophan hydroxylase."
      Darmon M.C., Guibert B., Leviel V., Ehret M., Maitre M., Mallet J.
      J. Neurochem. 51:312-316(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Pineal gland.
    2. "Molecular cloning and characterization of cDNA encoding tryptophan hydroxylase from rat central serotonergic neurons."
      Kim K.S., Wessel T.C., Stone D.M., Carver C.H., Joh T.H., Park D.H.
      Brain Res. Mol. Brain Res. 9:277-283(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Isolation of a rat pineal gland cDNA clone homologous to tyrosine and phenylalanine hydroxylases."
      Darmon M.C., Grima B., Cash C.D., Maitre M., Mallet J.
      FEBS Lett. 206:43-46(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 167-261.

    Entry informationi

    Entry nameiTPH1_RAT
    AccessioniPrimary (citable) accession number: P09810
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: October 1, 1989
    Last modified: October 1, 2014
    This is version 129 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3