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Protein

Tryptophan 5-hydroxylase 1

Gene

Tph1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.

Cofactori

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei235 – 2351TryptophanBy similarity
Binding sitei257 – 2571TryptophanBy similarity
Binding sitei265 – 2651TryptophanBy similarity
Metal bindingi272 – 2721IronBy similarity
Metal bindingi277 – 2771IronBy similarity
Metal bindingi317 – 3171IronBy similarity
Binding sitei336 – 3361TryptophanBy similarity
Binding sitei366 – 3661Tryptophan; via carbonyl oxygenBy similarity

GO - Molecular functioni

  1. amino acid binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. tryptophan 5-monooxygenase activity Source: RGD

GO - Biological processi

  1. bone remodeling Source: Ensembl
  2. circadian rhythm Source: RGD
  3. mammary gland alveolus development Source: Ensembl
  4. negative regulation of ossification Source: RGD
  5. response to immobilization stress Source: RGD
  6. serotonin biosynthetic process from tryptophan Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Serotonin biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_259603. Serotonin and melatonin biosynthesis.
SABIO-RKP09810.
UniPathwayiUPA00846; UER00799.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan 5-hydroxylase 1 (EC:1.14.16.4)
Alternative name(s):
Tryptophan 5-monooxygenase 1
Gene namesi
Name:Tph1
Synonyms:Tph
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi3895. Tph1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: RGD
  2. neuron projection Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444Tryptophan 5-hydroxylase 1PRO_0000205571Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei58 – 581Phosphoserine; by PKASequence Analysis

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP09810.
PRIDEiP09810.

PTM databases

PhosphoSiteiP09810.

Expressioni

Gene expression databases

GenevestigatoriP09810.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000052959.

Structurei

3D structure databases

ProteinModelPortaliP09810.
SMRiP09810. Positions 104-439.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 9476ACTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ACT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG3186.
GeneTreeiENSGT00390000010268.
HOGENOMiHOG000233373.
HOVERGENiHBG006841.
InParanoidiP09810.
KOiK00502.
OMAiQNVLMYG.
OrthoDBiEOG7KM5T1.
PhylomeDBiP09810.
TreeFamiTF313327.

Family and domain databases

Gene3Di1.10.800.10. 1 hit.
InterProiIPR002912. ACT_dom.
IPR001273. ArAA_hydroxylase.
IPR018301. ArAA_hydroxylase_Fe/CU_BS.
IPR019774. Aromatic-AA_hydroxylase_C.
IPR005963. Trp_5_mOase.
IPR019773. Tyrosine_3-monooxygenase-like.
[Graphical view]
PANTHERiPTHR11473. PTHR11473. 1 hit.
PfamiPF01842. ACT. 1 hit.
PF00351. Biopterin_H. 1 hit.
[Graphical view]
PIRSFiPIRSF000336. TH. 1 hit.
PRINTSiPR00372. FYWHYDRXLASE.
SUPFAMiSSF56534. SSF56534. 1 hit.
TIGRFAMsiTIGR01270. Trp_5_monoox. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
PS00367. BH4_AAA_HYDROXYL_1. 1 hit.
PS51410. BH4_AAA_HYDROXYL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09810-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIEDNKENKD HSSERGRVTL IFSLKNEVGG LIKALKIFQE NHVNLLHIES
60 70 80 90 100
RKSKRRNSEF EIFVDCDINR EQLNDIFPLL KSHTTVLSVD SPDQLPEKED
110 120 130 140 150
VMETVPWFPK KISDLDFCAN RVLLYGSELD ADHPGFKDNV YRRRRKYFAE
160 170 180 190 200
LAMNYKHGDP IPKIEFTEEE IKTWGTIFRE LNKLYPTHAC REYLRNLPLL
210 220 230 240 250
SKYCGYREDN VPQLEDVSNF LKERTGFSIR PVAGYLSPRD FLSGLAFRVF
260 270 280 290 300
HCTQYVRHSS DPLYTPEPDT CHELLGHVPL LAEPSFAQFS QEIGLASLGA
310 320 330 340 350
SEETVQKLAT CYFFTVEFGL CKQDGQLRVF GAGLLSSISE LRHALSGHAK
360 370 380 390 400
VKPFDPKVAC KQECLITSFQ DVYFVSESFE DAKEKMREFA KTVKRPFGVK
410 420 430 440
YNPYTQSIQV LRDSKSITSA MNELRHDLDV VNDALARVSR WPSV
Length:444
Mass (Da):51,068
Last modified:October 1, 1989 - v2
Checksum:iC3CF5245727CC825
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28000 mRNA. Translation: AAA42262.1.
X53501 mRNA. Translation: CAA37579.1.
PIRiJL0034. WHRTW.
RefSeqiNP_001094104.1. NM_001100634.2.
XP_006229281.1. XM_006229219.1.
UniGeneiRn.94788.

Genome annotation databases

EnsembliENSRNOT00000056109; ENSRNOP00000052959; ENSRNOG00000011672.
GeneIDi24848.
KEGGirno:24848.
UCSCiRGD:3895. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28000 mRNA. Translation: AAA42262.1.
X53501 mRNA. Translation: CAA37579.1.
PIRiJL0034. WHRTW.
RefSeqiNP_001094104.1. NM_001100634.2.
XP_006229281.1. XM_006229219.1.
UniGeneiRn.94788.

3D structure databases

ProteinModelPortaliP09810.
SMRiP09810. Positions 104-439.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000052959.

Chemistry

BindingDBiP09810.
ChEMBLiCHEMBL4809.

PTM databases

PhosphoSiteiP09810.

Proteomic databases

PaxDbiP09810.
PRIDEiP09810.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000056109; ENSRNOP00000052959; ENSRNOG00000011672.
GeneIDi24848.
KEGGirno:24848.
UCSCiRGD:3895. rat.

Organism-specific databases

CTDi7166.
RGDi3895. Tph1.

Phylogenomic databases

eggNOGiCOG3186.
GeneTreeiENSGT00390000010268.
HOGENOMiHOG000233373.
HOVERGENiHBG006841.
InParanoidiP09810.
KOiK00502.
OMAiQNVLMYG.
OrthoDBiEOG7KM5T1.
PhylomeDBiP09810.
TreeFamiTF313327.

Enzyme and pathway databases

UniPathwayiUPA00846; UER00799.
ReactomeiREACT_259603. Serotonin and melatonin biosynthesis.
SABIO-RKP09810.

Miscellaneous databases

NextBioi604610.
PROiP09810.

Gene expression databases

GenevestigatoriP09810.

Family and domain databases

Gene3Di1.10.800.10. 1 hit.
InterProiIPR002912. ACT_dom.
IPR001273. ArAA_hydroxylase.
IPR018301. ArAA_hydroxylase_Fe/CU_BS.
IPR019774. Aromatic-AA_hydroxylase_C.
IPR005963. Trp_5_mOase.
IPR019773. Tyrosine_3-monooxygenase-like.
[Graphical view]
PANTHERiPTHR11473. PTHR11473. 1 hit.
PfamiPF01842. ACT. 1 hit.
PF00351. Biopterin_H. 1 hit.
[Graphical view]
PIRSFiPIRSF000336. TH. 1 hit.
PRINTSiPR00372. FYWHYDRXLASE.
SUPFAMiSSF56534. SSF56534. 1 hit.
TIGRFAMsiTIGR01270. Trp_5_monoox. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
PS00367. BH4_AAA_HYDROXYL_1. 1 hit.
PS51410. BH4_AAA_HYDROXYL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence of two mRNAs encoding active rat tryptophan hydroxylase."
    Darmon M.C., Guibert B., Leviel V., Ehret M., Maitre M., Mallet J.
    J. Neurochem. 51:312-316(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Pineal gland.
  2. "Molecular cloning and characterization of cDNA encoding tryptophan hydroxylase from rat central serotonergic neurons."
    Kim K.S., Wessel T.C., Stone D.M., Carver C.H., Joh T.H., Park D.H.
    Brain Res. Mol. Brain Res. 9:277-283(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Isolation of a rat pineal gland cDNA clone homologous to tyrosine and phenylalanine hydroxylases."
    Darmon M.C., Grima B., Cash C.D., Maitre M., Mallet J.
    FEBS Lett. 206:43-46(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 167-261.

Entry informationi

Entry nameiTPH1_RAT
AccessioniPrimary (citable) accession number: P09810
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 1, 1989
Last modified: January 7, 2015
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.