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P09805

- KTXA_KLULA

UniProt

P09805 - KTXA_KLULA

Protein

Killer toxin subunits alpha/beta

Gene
N/A
Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    The alpha subunit is a potent exochitinase. Along with the beta subunit it plays a role in the initial interaction of the toxin with sensitive cells and allow the gamma subunit (the active toxin) to gain entry into the cell.

    Catalytic activityi

    Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei495 – 4951Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. chitinase activity Source: UniProtKB-EC
    2. chitin binding Source: UniProtKB-KW

    GO - Biological processi

    1. chitin catabolic process Source: UniProtKB-KW
    2. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Toxin

    Keywords - Biological processi

    Carbohydrate metabolism, Chitin degradation, Polysaccharide degradation

    Keywords - Ligandi

    Chitin-binding

    Protein family/group databases

    CAZyiCBM18. Carbohydrate-Binding Module Family 18.
    CBM50. Carbohydrate-Binding Module Family 50.
    GH18. Glycoside Hydrolase Family 18.
    mycoCLAPiCHI18A_KLULA.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Killer toxin subunits alpha/beta
    Alternative name(s):
    RF2 protein
    Cleaved into the following 2 chains:
    Encoded oniPlasmid pGKl-10 Publication
    OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
    Taxonomic identifieri284590 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
    ProteomesiUP000000598: Plasmid pGKL1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Propeptidei18 – 2912Sequence AnalysisPRO_0000011938Add
    BLAST
    Chaini30 – 892863Killer toxin subunit alphaCuratedPRO_0000011939Add
    BLAST
    Chaini895 – 1146252Killer toxin subunit betaCuratedPRO_0000011940Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi319 ↔ 338PROSITE-ProRule annotation
    Disulfide bondi332 ↔ 344PROSITE-ProRule annotation
    Disulfide bondi337 ↔ 351PROSITE-ProRule annotation
    Disulfide bondi366 ↔ 370PROSITE-ProRule annotation
    Glycosylationi771 – 7711N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi858 – 8581N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi868 – 8681N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi876 – 8761N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi995 – 9951N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1082 – 10821N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1117 – 11171N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    RF2 is potentially split by membrane-bound basic amino acid-specific peptidase to yield the alpha and beta subunits.

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    The killer toxin is composed of three subunits: alpha, beta and gamma.

    Structurei

    3D structure databases

    ProteinModelPortaliP09805.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati205 – 23430LysM 1Add
    BLAST
    Repeati256 – 28530LysM 2Add
    BLAST
    Domaini316 – 37257Chitin-binding type-1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 chitin-binding type-1 domain.PROSITE-ProRule annotation
    Contains 2 LysM repeats.Curated

    Keywords - Domaini

    Repeat, Signal

    Family and domain databases

    Gene3Di3.10.50.10. 1 hit.
    3.20.20.80. 2 hits.
    3.30.60.10. 1 hit.
    InterProiIPR001002. Chitin-bd_1.
    IPR018371. Chitin-binding_1_CS.
    IPR011583. Chitinase_II.
    IPR029070. Chitinase_insertion.
    IPR001223. Glyco_hydro18cat.
    IPR001579. Glyco_hydro_18_chit_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR018392. LysM_dom.
    [Graphical view]
    PfamiPF00187. Chitin_bind_1. 1 hit.
    PF00704. Glyco_hydro_18. 1 hit.
    PF01476. LysM. 1 hit.
    [Graphical view]
    ProDomiPD000609. Chitin_bd_1. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00270. ChtBD1. 1 hit.
    SM00636. Glyco_18. 1 hit.
    SM00257. LysM. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 2 hits.
    SSF54106. SSF54106. 1 hit.
    SSF54556. SSF54556. 1 hit.
    SSF57016. SSF57016. 1 hit.
    PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
    PS50941. CHIT_BIND_I_2. 1 hit.
    PS01095. CHITINASE_18. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09805-1 [UniParc]FASTAAdd to Basket

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    MNIFYIFLFL LSFVQGLEHT HRRGSLVKRA VCYDTDQVPL NIFFGYNRAD     50
    KTDSNKNMAL NIFNVFRGFL AGEGGESFYN SNGNVYGFMW VGSMVHNRGF 100
    KDNILPIMEN EVKNYGIPKT LYLEYDGGGD PMKSFGIILD TTSRDTVVKA 150
    AKLWSQGKKL NSYEGSKNYQ ATACYLSYAY RKPIVNDNFV GTCDYFTLES 200
    GKTPADQSGI NGESLQGYNP NLDFSKLSAG QPICKTIGNP PNFKPSKNSD 250
    GSCKTYKVSS GESCSSIAVK YYPLSLNDIE NYNKGNYGWK GCSSLQKDYN 300
    LCVSDGSAPR PVSNPIAECG PLAPGEKYNA KCPLNACCSE FGFCGLTKDY 350
    CDKKSSTTGA PGTDGCFSNC GYGSTSNVKS STFKKIAYWL DAKDKLAMDP 400
    KNIPNGPYDI LHYAFVNINS DFSIDDSAFS KSAFLKVTSS KKIPSFGGWD 450
    FSTSPSTYTI FRNAVKTDQN RNTFANNLIN FMNKYNLDGI DLDWEYPGAP 500
    DIPDIPADDS SSGSNYLTFL KLLKGKMPSG KTLSIAIPSS YWYLKNFPIS 550
    DIQNTVDYMV YMTYDIHGIW EYGKANSYIN CHTPRKEIED AIKMLDKAGV 600
    KFNKVFGGVA NYGRSYKMVN TNCYNYGCGF QREGGNSRDM TNTPGVLSDS 650
    EIIDIDSSDK KNDRWVDTNT DCIFMKYDGN SVVSWPKSRY DLEDMFKNYG 700
    FAGTSLWAAN YFKHDEWKND EDDNNDDTED PFDEENVYFD VYDCKNKAGY 750
    DLDNPVYGCR LETAINIIIW NGTESVNTVL NILNDYDNYI KYYEALTRAH 800
    YDSVMEKYEK WLFEEDGYYT YYTDVDGDDI IITPPDKKKR DYIQEKYSFE 850
    KEFMMSQNMT ELTEIKVNKT INFMLNGTSL AVKEYNNEKV LYKRGDIPPP 900
    GSNNRLIRNS IILDKDKEAA IASFKQYSGI ELSKDSFVQR DKDKKFDLNG 950
    KHYTFMHSTI LNAIVLFPNV LTNIDSDYIH HISDLIEQAH NSLGNESPDN 1000
    IYEVLESVVV FMSVSEIADY TYTEGKKIKE KYDKMKKTMI VGIILGIIGG 1050
    LSLFLGPIGI ATSVLADFAL LGADAAINGE LNPSDLAFAL AGLFLPVFAS 1100
    LGKTFKFAEA LQKININKSK NFDNLNEFEK IRFFRSKLGK VKMCGS 1146
    Length:1,146
    Mass (Da):128,937
    Last modified:July 1, 1989 - v1
    Checksum:iBF4B1764EB465DE6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07127 Genomic DNA. Translation: CAA30137.1.
    X00762 Genomic DNA. Translation: CAA25334.1.
    X01095 Genomic DNA. Translation: CAA25569.1.
    PIRiS07915.
    RefSeqiYP_001648054.1. NC_010185.1.
    YP_001648058.1. NC_010186.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07127 Genomic DNA. Translation: CAA30137.1 .
    X00762 Genomic DNA. Translation: CAA25334.1 .
    X01095 Genomic DNA. Translation: CAA25569.1 .
    PIRi S07915.
    RefSeqi YP_001648054.1. NC_010185.1.
    YP_001648058.1. NC_010186.1.

    3D structure databases

    ProteinModelPortali P09805.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM18. Carbohydrate-Binding Module Family 18.
    CBM50. Carbohydrate-Binding Module Family 50.
    GH18. Glycoside Hydrolase Family 18.
    mycoCLAPi CHI18A_KLULA.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.10.50.10. 1 hit.
    3.20.20.80. 2 hits.
    3.30.60.10. 1 hit.
    InterProi IPR001002. Chitin-bd_1.
    IPR018371. Chitin-binding_1_CS.
    IPR011583. Chitinase_II.
    IPR029070. Chitinase_insertion.
    IPR001223. Glyco_hydro18cat.
    IPR001579. Glyco_hydro_18_chit_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR018392. LysM_dom.
    [Graphical view ]
    Pfami PF00187. Chitin_bind_1. 1 hit.
    PF00704. Glyco_hydro_18. 1 hit.
    PF01476. LysM. 1 hit.
    [Graphical view ]
    ProDomi PD000609. Chitin_bd_1. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00270. ChtBD1. 1 hit.
    SM00636. Glyco_18. 1 hit.
    SM00257. LysM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 2 hits.
    SSF54106. SSF54106. 1 hit.
    SSF54556. SSF54556. 1 hit.
    SSF57016. SSF57016. 1 hit.
    PROSITEi PS00026. CHIT_BIND_I_1. 1 hit.
    PS50941. CHIT_BIND_I_2. 1 hit.
    PS01095. CHITINASE_18. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure of a linear plasmid of the yeast Kluyveromyces lactis; compact organization of the killer genome."
      Sor F., Fukuhara H.
      Curr. Genet. 9:147-155(1985)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 76492 / CBS 2359/152 / CLIB 210.
    2. "Nucleotide sequence and transcription analysis of a linear DNA plasmid associated with the killer character of the yeast Kluyveromyces lactis."
      Stark M.J.R., Mileham A.J., Romanos M.A., Boyd A.
      Nucleic Acids Res. 12:6011-6030(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.
    3. "Cloning and nucleotide sequences of the linear DNA killer plasmids from yeast."
      Hishinuma F., Nakamura K., Hirai K., Nishizawa R., Gunge N., Maeda T.
      Nucleic Acids Res. 12:7581-7597(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 52735 / 2105-1D.
    4. "The killer toxin of Kluyveromyces lactis: characterization of the toxin subunits and identification of the genes which encode them."
      Stark M.J.R., Boyd A.
      EMBO J. 5:1995-2002(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF PROTEIN, PROTEIN SEQUENCE OF 30-44 AND 895-916.
    5. Cited for: SIMILARITY TO CHITINASE OF ALPHA-SUBUNIT.
    6. "Kluyveromyces lactis toxin has an essential chitinase activity."
      Butler A.R., O'Donnell R.W., Martin V.J., Gooday G.W., Stark M.J.R.
      Eur. J. Biochem. 199:483-488(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHITINASE ACTIVITY OF ALPHA-SUBUNIT.

    Entry informationi

    Entry nameiKTXA_KLULA
    AccessioniPrimary (citable) accession number: P09805
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3