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P09805

- KTXA_KLULA

UniProt

P09805 - KTXA_KLULA

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Protein
Killer toxin subunits alpha/beta
Gene
N/A
Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

The alpha subunit is a potent exochitinase. Along with the beta subunit it plays a role in the initial interaction of the toxin with sensitive cells and allow the gamma subunit (the active toxin) to gain entry into the cell.

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei495 – 4951Proton donor By similarity

GO - Molecular functioni

  1. chitin binding Source: UniProtKB-KW
  2. chitinase activity Source: UniProtKB-EC

GO - Biological processi

  1. chitin catabolic process Source: UniProtKB-KW
  2. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Toxin

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Polysaccharide degradation

Keywords - Ligandi

Chitin-binding

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
CBM50. Carbohydrate-Binding Module Family 50.
GH18. Glycoside Hydrolase Family 18.
mycoCLAPiCHI18A_KLULA.

Names & Taxonomyi

Protein namesi
Recommended name:
Killer toxin subunits alpha/beta
Alternative name(s):
RF2 protein
Cleaved into the following 2 chains:
Encoded oniPlasmid pGKl-10 Publication
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
ProteomesiUP000000598: Plasmid pGKL1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717 Reviewed prediction
Add
BLAST
Propeptidei18 – 2912 Reviewed prediction
PRO_0000011938Add
BLAST
Chaini30 – 892863Killer toxin subunit alpha Inferred
PRO_0000011939Add
BLAST
Chaini895 – 1146252Killer toxin subunit beta Inferred
PRO_0000011940Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi319 ↔ 338 By similarity
Disulfide bondi332 ↔ 344 By similarity
Disulfide bondi337 ↔ 351 By similarity
Disulfide bondi366 ↔ 370 By similarity
Glycosylationi771 – 7711N-linked (GlcNAc...) Reviewed prediction
Glycosylationi858 – 8581N-linked (GlcNAc...) Reviewed prediction
Glycosylationi868 – 8681N-linked (GlcNAc...) Reviewed prediction
Glycosylationi876 – 8761N-linked (GlcNAc...) Reviewed prediction
Glycosylationi995 – 9951N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1082 – 10821N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1117 – 11171N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

RF2 is potentially split by membrane-bound basic amino acid-specific peptidase to yield the alpha and beta subunits.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

The killer toxin is composed of three subunits: alpha, beta and gamma.2 Publications

Structurei

3D structure databases

ProteinModelPortaliP09805.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati205 – 23430LysM 1
Add
BLAST
Repeati256 – 28530LysM 2
Add
BLAST
Domaini316 – 37257Chitin-binding type-1
Add
BLAST

Sequence similaritiesi

Contains 2 LysM repeats.

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
3.30.60.10. 1 hit.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR001223. Glyco_hydro18cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR018392. LysM_dom.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
PF01476. LysM. 1 hit.
[Graphical view]
ProDomiPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 1 hit.
SM00636. Glyco_18. 1 hit.
SM00257. LysM. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF54106. SSF54106. 1 hit.
SSF54556. SSF54556. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS01095. CHITINASE_18. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09805-1 [UniParc]FASTAAdd to Basket

« Hide

MNIFYIFLFL LSFVQGLEHT HRRGSLVKRA VCYDTDQVPL NIFFGYNRAD     50
KTDSNKNMAL NIFNVFRGFL AGEGGESFYN SNGNVYGFMW VGSMVHNRGF 100
KDNILPIMEN EVKNYGIPKT LYLEYDGGGD PMKSFGIILD TTSRDTVVKA 150
AKLWSQGKKL NSYEGSKNYQ ATACYLSYAY RKPIVNDNFV GTCDYFTLES 200
GKTPADQSGI NGESLQGYNP NLDFSKLSAG QPICKTIGNP PNFKPSKNSD 250
GSCKTYKVSS GESCSSIAVK YYPLSLNDIE NYNKGNYGWK GCSSLQKDYN 300
LCVSDGSAPR PVSNPIAECG PLAPGEKYNA KCPLNACCSE FGFCGLTKDY 350
CDKKSSTTGA PGTDGCFSNC GYGSTSNVKS STFKKIAYWL DAKDKLAMDP 400
KNIPNGPYDI LHYAFVNINS DFSIDDSAFS KSAFLKVTSS KKIPSFGGWD 450
FSTSPSTYTI FRNAVKTDQN RNTFANNLIN FMNKYNLDGI DLDWEYPGAP 500
DIPDIPADDS SSGSNYLTFL KLLKGKMPSG KTLSIAIPSS YWYLKNFPIS 550
DIQNTVDYMV YMTYDIHGIW EYGKANSYIN CHTPRKEIED AIKMLDKAGV 600
KFNKVFGGVA NYGRSYKMVN TNCYNYGCGF QREGGNSRDM TNTPGVLSDS 650
EIIDIDSSDK KNDRWVDTNT DCIFMKYDGN SVVSWPKSRY DLEDMFKNYG 700
FAGTSLWAAN YFKHDEWKND EDDNNDDTED PFDEENVYFD VYDCKNKAGY 750
DLDNPVYGCR LETAINIIIW NGTESVNTVL NILNDYDNYI KYYEALTRAH 800
YDSVMEKYEK WLFEEDGYYT YYTDVDGDDI IITPPDKKKR DYIQEKYSFE 850
KEFMMSQNMT ELTEIKVNKT INFMLNGTSL AVKEYNNEKV LYKRGDIPPP 900
GSNNRLIRNS IILDKDKEAA IASFKQYSGI ELSKDSFVQR DKDKKFDLNG 950
KHYTFMHSTI LNAIVLFPNV LTNIDSDYIH HISDLIEQAH NSLGNESPDN 1000
IYEVLESVVV FMSVSEIADY TYTEGKKIKE KYDKMKKTMI VGIILGIIGG 1050
LSLFLGPIGI ATSVLADFAL LGADAAINGE LNPSDLAFAL AGLFLPVFAS 1100
LGKTFKFAEA LQKININKSK NFDNLNEFEK IRFFRSKLGK VKMCGS 1146
Length:1,146
Mass (Da):128,937
Last modified:July 1, 1989 - v1
Checksum:iBF4B1764EB465DE6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X07127 Genomic DNA. Translation: CAA30137.1.
X00762 Genomic DNA. Translation: CAA25334.1.
X01095 Genomic DNA. Translation: CAA25569.1.
PIRiS07915.
RefSeqiYP_001648054.1. NC_010185.1.
YP_001648058.1. NC_010186.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X07127 Genomic DNA. Translation: CAA30137.1 .
X00762 Genomic DNA. Translation: CAA25334.1 .
X01095 Genomic DNA. Translation: CAA25569.1 .
PIRi S07915.
RefSeqi YP_001648054.1. NC_010185.1.
YP_001648058.1. NC_010186.1.

3D structure databases

ProteinModelPortali P09805.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM18. Carbohydrate-Binding Module Family 18.
CBM50. Carbohydrate-Binding Module Family 50.
GH18. Glycoside Hydrolase Family 18.
mycoCLAPi CHI18A_KLULA.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
3.30.60.10. 1 hit.
InterProi IPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR001223. Glyco_hydro18cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR018392. LysM_dom.
[Graphical view ]
Pfami PF00187. Chitin_bind_1. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
PF01476. LysM. 1 hit.
[Graphical view ]
ProDomi PD000609. Chitin_bd_1. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00270. ChtBD1. 1 hit.
SM00636. Glyco_18. 1 hit.
SM00257. LysM. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 2 hits.
SSF54106. SSF54106. 1 hit.
SSF54556. SSF54556. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEi PS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS01095. CHITINASE_18. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Structure of a linear plasmid of the yeast Kluyveromyces lactis; compact organization of the killer genome."
    Sor F., Fukuhara H.
    Curr. Genet. 9:147-155(1985)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 76492 / CBS 2359/152 / CLIB 210.
  2. "Nucleotide sequence and transcription analysis of a linear DNA plasmid associated with the killer character of the yeast Kluyveromyces lactis."
    Stark M.J.R., Mileham A.J., Romanos M.A., Boyd A.
    Nucleic Acids Res. 12:6011-6030(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.
  3. "Cloning and nucleotide sequences of the linear DNA killer plasmids from yeast."
    Hishinuma F., Nakamura K., Hirai K., Nishizawa R., Gunge N., Maeda T.
    Nucleic Acids Res. 12:7581-7597(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 52735 / 2105-1D.
  4. "The killer toxin of Kluyveromyces lactis: characterization of the toxin subunits and identification of the genes which encode them."
    Stark M.J.R., Boyd A.
    EMBO J. 5:1995-2002(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF PROTEIN, PROTEIN SEQUENCE OF 30-44 AND 895-916.
  5. Cited for: SIMILARITY TO CHITINASE OF ALPHA-SUBUNIT.
  6. "Kluyveromyces lactis toxin has an essential chitinase activity."
    Butler A.R., O'Donnell R.W., Martin V.J., Gooday G.W., Stark M.J.R.
    Eur. J. Biochem. 199:483-488(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHITINASE ACTIVITY OF ALPHA-SUBUNIT.

Entry informationi

Entry nameiKTXA_KLULA
AccessioniPrimary (citable) accession number: P09805
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: September 3, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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