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Protein

Killer toxin subunits alpha/beta

Gene
N/A
Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The alpha subunit is a potent exochitinase. Along with the beta subunit it plays a role in the initial interaction of the toxin with sensitive cells and allow the gamma subunit (the active toxin) to gain entry into the cell.

Catalytic activityi

Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei495Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase, Toxin
Biological processCarbohydrate metabolism, Chitin degradation, Polysaccharide degradation
LigandChitin-binding

Protein family/group databases

CAZyiCBM18 Carbohydrate-Binding Module Family 18
CBM50 Carbohydrate-Binding Module Family 50
GH18 Glycoside Hydrolase Family 18
mycoCLAPiCHI18A_KLULA

Names & Taxonomyi

Protein namesi
Recommended name:
Killer toxin subunits alpha/beta
Alternative name(s):
RF2 protein
Cleaved into the following 2 chains:
Encoded oniPlasmid pGKl-10 Publication
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Plasmid pGKL1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
PropeptideiPRO_000001193818 – 29Sequence analysisAdd BLAST12
ChainiPRO_000001193930 – 892Killer toxin subunit alphaCuratedAdd BLAST863
ChainiPRO_0000011940895 – 1146Killer toxin subunit betaCuratedAdd BLAST252

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi319 ↔ 338PROSITE-ProRule annotation
Disulfide bondi332 ↔ 344PROSITE-ProRule annotation
Disulfide bondi337 ↔ 351PROSITE-ProRule annotation
Disulfide bondi366 ↔ 370PROSITE-ProRule annotation
Glycosylationi771N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi858N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi868N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi876N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi995N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1082N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1117N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

RF2 is potentially split by membrane-bound basic amino acid-specific peptidase to yield the alpha and beta subunits.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP09805

Interactioni

Subunit structurei

The killer toxin is composed of three subunits: alpha, beta and gamma.

Structurei

3D structure databases

ProteinModelPortaliP09805
SMRiP09805
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini205 – 234LysM 1Add BLAST30
Domaini254 – 303LysM 2PROSITE-ProRule annotationAdd BLAST50
Domaini316 – 372Chitin-binding type-1PROSITE-ProRule annotationAdd BLAST57

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

OrthoDBiEOG092C04AM

Family and domain databases

CDDicd00118 LysM, 1 hit
Gene3Di3.10.350.10, 1 hit
3.10.50.10, 1 hit
3.30.60.10, 1 hit
InterProiView protein in InterPro
IPR001002 Chitin-bd_1
IPR018371 Chitin-binding_1_CS
IPR011583 Chitinase_II
IPR029070 Chitinase_insertion_sf
IPR036861 Endochitinase-like_sf
IPR001223 Glyco_hydro18_cat
IPR001579 Glyco_hydro_18_chit_AS
IPR017853 Glycoside_hydrolase_SF
IPR018392 LysM_dom
IPR036779 LysM_dom_sf
PfamiView protein in Pfam
PF00187 Chitin_bind_1, 1 hit
PF00704 Glyco_hydro_18, 1 hit
PF01476 LysM, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD000609 Chitin_bd_1, 1 hit
SMARTiView protein in SMART
SM00270 ChtBD1, 1 hit
SM00636 Glyco_18, 1 hit
SM00257 LysM, 1 hit
SUPFAMiSSF51445 SSF51445, 2 hits
SSF54556 SSF54556, 1 hit
SSF57016 SSF57016, 1 hit
PROSITEiView protein in PROSITE
PS00026 CHIT_BIND_I_1, 1 hit
PS50941 CHIT_BIND_I_2, 1 hit
PS01095 CHITINASE_18, 1 hit
PS51782 LYSM, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09805-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIFYIFLFL LSFVQGLEHT HRRGSLVKRA VCYDTDQVPL NIFFGYNRAD
60 70 80 90 100
KTDSNKNMAL NIFNVFRGFL AGEGGESFYN SNGNVYGFMW VGSMVHNRGF
110 120 130 140 150
KDNILPIMEN EVKNYGIPKT LYLEYDGGGD PMKSFGIILD TTSRDTVVKA
160 170 180 190 200
AKLWSQGKKL NSYEGSKNYQ ATACYLSYAY RKPIVNDNFV GTCDYFTLES
210 220 230 240 250
GKTPADQSGI NGESLQGYNP NLDFSKLSAG QPICKTIGNP PNFKPSKNSD
260 270 280 290 300
GSCKTYKVSS GESCSSIAVK YYPLSLNDIE NYNKGNYGWK GCSSLQKDYN
310 320 330 340 350
LCVSDGSAPR PVSNPIAECG PLAPGEKYNA KCPLNACCSE FGFCGLTKDY
360 370 380 390 400
CDKKSSTTGA PGTDGCFSNC GYGSTSNVKS STFKKIAYWL DAKDKLAMDP
410 420 430 440 450
KNIPNGPYDI LHYAFVNINS DFSIDDSAFS KSAFLKVTSS KKIPSFGGWD
460 470 480 490 500
FSTSPSTYTI FRNAVKTDQN RNTFANNLIN FMNKYNLDGI DLDWEYPGAP
510 520 530 540 550
DIPDIPADDS SSGSNYLTFL KLLKGKMPSG KTLSIAIPSS YWYLKNFPIS
560 570 580 590 600
DIQNTVDYMV YMTYDIHGIW EYGKANSYIN CHTPRKEIED AIKMLDKAGV
610 620 630 640 650
KFNKVFGGVA NYGRSYKMVN TNCYNYGCGF QREGGNSRDM TNTPGVLSDS
660 670 680 690 700
EIIDIDSSDK KNDRWVDTNT DCIFMKYDGN SVVSWPKSRY DLEDMFKNYG
710 720 730 740 750
FAGTSLWAAN YFKHDEWKND EDDNNDDTED PFDEENVYFD VYDCKNKAGY
760 770 780 790 800
DLDNPVYGCR LETAINIIIW NGTESVNTVL NILNDYDNYI KYYEALTRAH
810 820 830 840 850
YDSVMEKYEK WLFEEDGYYT YYTDVDGDDI IITPPDKKKR DYIQEKYSFE
860 870 880 890 900
KEFMMSQNMT ELTEIKVNKT INFMLNGTSL AVKEYNNEKV LYKRGDIPPP
910 920 930 940 950
GSNNRLIRNS IILDKDKEAA IASFKQYSGI ELSKDSFVQR DKDKKFDLNG
960 970 980 990 1000
KHYTFMHSTI LNAIVLFPNV LTNIDSDYIH HISDLIEQAH NSLGNESPDN
1010 1020 1030 1040 1050
IYEVLESVVV FMSVSEIADY TYTEGKKIKE KYDKMKKTMI VGIILGIIGG
1060 1070 1080 1090 1100
LSLFLGPIGI ATSVLADFAL LGADAAINGE LNPSDLAFAL AGLFLPVFAS
1110 1120 1130 1140
LGKTFKFAEA LQKININKSK NFDNLNEFEK IRFFRSKLGK VKMCGS
Length:1,146
Mass (Da):128,937
Last modified:July 1, 1989 - v1
Checksum:iBF4B1764EB465DE6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07127 Genomic DNA Translation: CAA30137.1
X00762 Genomic DNA Translation: CAA25334.1
X01095 Genomic DNA Translation: CAA25569.1
PIRiS07915

Similar proteinsi

Entry informationi

Entry nameiKTXA_KLULA
AccessioniPrimary (citable) accession number: P09805
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: February 28, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

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