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P09805 (KTXA_KLULA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Killer toxin subunits alpha/beta
Alternative name(s):
RF2 protein

Cleaved into the following 2 chains:

  1. Killer toxin subunit alpha
  2. Killer toxin subunit beta
    EC=3.2.1.14
    Alternative name(s):
    Endochitinase
Encoded onPlasmid pGKl-1
OrganismKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) [Complete proteome]
Taxonomic identifier284590 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces

Protein attributes

Sequence length1146 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The alpha subunit is a potent exochitinase. Along with the beta subunit it plays a role in the initial interaction of the toxin with sensitive cells and allow the gamma subunit (the active toxin) to gain entry into the cell.

Catalytic activity

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Subunit structure

The killer toxin is composed of three subunits: alpha, beta and gamma. Ref.5 Ref.6

Post-translational modification

RF2 is potentially split by membrane-bound basic amino acid-specific peptidase to yield the alpha and beta subunits.

Sequence similarities

Contains 1 chitin-binding type-1 domain.

Contains 2 LysM repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Propeptide18 – 2912 Potential
PRO_0000011938
Chain30 – 892863Killer toxin subunit alpha Probable
PRO_0000011939
Chain895 – 1146252Killer toxin subunit beta Probable
PRO_0000011940

Regions

Repeat205 – 23430LysM 1
Repeat256 – 28530LysM 2
Domain316 – 37257Chitin-binding type-1

Sites

Active site4951Proton donor By similarity

Amino acid modifications

Glycosylation7711N-linked (GlcNAc...) Potential
Glycosylation8581N-linked (GlcNAc...) Potential
Glycosylation8681N-linked (GlcNAc...) Potential
Glycosylation8761N-linked (GlcNAc...) Potential
Glycosylation9951N-linked (GlcNAc...) Potential
Glycosylation10821N-linked (GlcNAc...) Potential
Glycosylation11171N-linked (GlcNAc...) Potential
Disulfide bond319 ↔ 338 By similarity
Disulfide bond332 ↔ 344 By similarity
Disulfide bond337 ↔ 351 By similarity
Disulfide bond366 ↔ 370 By similarity

Sequences

Sequence LengthMass (Da)Tools
P09805 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: BF4B1764EB465DE6

FASTA1,146128,937
        10         20         30         40         50         60 
MNIFYIFLFL LSFVQGLEHT HRRGSLVKRA VCYDTDQVPL NIFFGYNRAD KTDSNKNMAL 

        70         80         90        100        110        120 
NIFNVFRGFL AGEGGESFYN SNGNVYGFMW VGSMVHNRGF KDNILPIMEN EVKNYGIPKT 

       130        140        150        160        170        180 
LYLEYDGGGD PMKSFGIILD TTSRDTVVKA AKLWSQGKKL NSYEGSKNYQ ATACYLSYAY 

       190        200        210        220        230        240 
RKPIVNDNFV GTCDYFTLES GKTPADQSGI NGESLQGYNP NLDFSKLSAG QPICKTIGNP 

       250        260        270        280        290        300 
PNFKPSKNSD GSCKTYKVSS GESCSSIAVK YYPLSLNDIE NYNKGNYGWK GCSSLQKDYN 

       310        320        330        340        350        360 
LCVSDGSAPR PVSNPIAECG PLAPGEKYNA KCPLNACCSE FGFCGLTKDY CDKKSSTTGA 

       370        380        390        400        410        420 
PGTDGCFSNC GYGSTSNVKS STFKKIAYWL DAKDKLAMDP KNIPNGPYDI LHYAFVNINS 

       430        440        450        460        470        480 
DFSIDDSAFS KSAFLKVTSS KKIPSFGGWD FSTSPSTYTI FRNAVKTDQN RNTFANNLIN 

       490        500        510        520        530        540 
FMNKYNLDGI DLDWEYPGAP DIPDIPADDS SSGSNYLTFL KLLKGKMPSG KTLSIAIPSS 

       550        560        570        580        590        600 
YWYLKNFPIS DIQNTVDYMV YMTYDIHGIW EYGKANSYIN CHTPRKEIED AIKMLDKAGV 

       610        620        630        640        650        660 
KFNKVFGGVA NYGRSYKMVN TNCYNYGCGF QREGGNSRDM TNTPGVLSDS EIIDIDSSDK 

       670        680        690        700        710        720 
KNDRWVDTNT DCIFMKYDGN SVVSWPKSRY DLEDMFKNYG FAGTSLWAAN YFKHDEWKND 

       730        740        750        760        770        780 
EDDNNDDTED PFDEENVYFD VYDCKNKAGY DLDNPVYGCR LETAINIIIW NGTESVNTVL 

       790        800        810        820        830        840 
NILNDYDNYI KYYEALTRAH YDSVMEKYEK WLFEEDGYYT YYTDVDGDDI IITPPDKKKR 

       850        860        870        880        890        900 
DYIQEKYSFE KEFMMSQNMT ELTEIKVNKT INFMLNGTSL AVKEYNNEKV LYKRGDIPPP 

       910        920        930        940        950        960 
GSNNRLIRNS IILDKDKEAA IASFKQYSGI ELSKDSFVQR DKDKKFDLNG KHYTFMHSTI 

       970        980        990       1000       1010       1020 
LNAIVLFPNV LTNIDSDYIH HISDLIEQAH NSLGNESPDN IYEVLESVVV FMSVSEIADY 

      1030       1040       1050       1060       1070       1080 
TYTEGKKIKE KYDKMKKTMI VGIILGIIGG LSLFLGPIGI ATSVLADFAL LGADAAINGE 

      1090       1100       1110       1120       1130       1140 
LNPSDLAFAL AGLFLPVFAS LGKTFKFAEA LQKININKSK NFDNLNEFEK IRFFRSKLGK 


VKMCGS 

« Hide

References

[1]"Structure of a linear plasmid of the yeast Kluyveromyces lactis; compact organization of the killer genome."
Sor F., Fukuhara H.
Curr. Genet. 9:147-155(1985)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 76492 / CBS 2359/152 / CLIB 210.
[2]"Nucleotide sequence and transcription analysis of a linear DNA plasmid associated with the killer character of the yeast Kluyveromyces lactis."
Stark M.J.R., Mileham A.J., Romanos M.A., Boyd A.
Nucleic Acids Res. 12:6011-6030(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.
[3]"Cloning and nucleotide sequences of the linear DNA killer plasmids from yeast."
Hishinuma F., Nakamura K., Hirai K., Nishizawa R., Gunge N., Maeda T.
Nucleic Acids Res. 12:7581-7597(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 52735 / 2105-1D.
[4]"The killer toxin of Kluyveromyces lactis: characterization of the toxin subunits and identification of the genes which encode them."
Stark M.J.R., Boyd A.
EMBO J. 5:1995-2002(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF PROTEIN, PROTEIN SEQUENCE OF 30-44 AND 895-916.
[5]"Killer toxins."
Bradshaw H.D. Jr.
Nature 345:299-299(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: SIMILARITY TO CHITINASE OF ALPHA-SUBUNIT.
[6]"Kluyveromyces lactis toxin has an essential chitinase activity."
Butler A.R., O'Donnell R.W., Martin V.J., Gooday G.W., Stark M.J.R.
Eur. J. Biochem. 199:483-488(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: CHITINASE ACTIVITY OF ALPHA-SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X07127 Genomic DNA. Translation: CAA30137.1.
X00762 Genomic DNA. Translation: CAA25334.1.
X01095 Genomic DNA. Translation: CAA25569.1.
PIRS07915.
RefSeqYP_001648054.1. NC_010185.1.
YP_001648058.1. NC_010186.1.

3D structure databases

ProteinModelPortalP09805.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM18. Carbohydrate-Binding Module Family 18.
CBM50. Carbohydrate-Binding Module Family 50.
GH18. Glycoside Hydrolase Family 18.
mycoCLAPCHI18A_KLULA.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
3.30.60.10. 1 hit.
InterProIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR001223. Glyco_hydro18cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR018392. LysM_dom.
[Graphical view]
PfamPF00187. Chitin_bind_1. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
PF01476. LysM. 1 hit.
[Graphical view]
ProDomPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00270. ChtBD1. 1 hit.
SM00636. Glyco_18. 1 hit.
SM00257. LysM. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 2 hits.
SSF54106. SSF54106. 1 hit.
SSF54556. SSF54556. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS01095. CHITINASE_18. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKTXA_KLULA
AccessionPrimary (citable) accession number: P09805
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 11, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families