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Protein

Anaphase-promoting complex subunit CDC16

Gene

CDC16

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. In early mitosis, the APC/C is activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5, and other anaphase inhibitory proteins for proteolysis, thereby triggering the separation of sister chromatids at the metaphase-to-anaphase transition. In late mitosis and in G1, degradation of CLB5 allows activation of the APC/C by CDH1, which is needed to destroy CDC20 and the B-type cyclin CLB2 to allow exit from mitosis and creating the low CDK state necessary for cytokinesis and for reforming prereplicative complexes in G1 prior to another round of replication.5 Publications

Miscellaneous

Present with 2753 molecules/cell in log phase SD medium.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • anaphase-promoting complex-dependent catabolic process Source: SGD
  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • negative regulation of DNA-dependent DNA replication initiation Source: SGD
  • protein ubiquitination Source: SGD

Keywordsi

Biological processCell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-31830-MONOMER
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Anaphase-promoting complex subunit CDC16
Alternative name(s):
Cell division control protein 16
Gene namesi
Name:CDC16
Ordered Locus Names:YKL022C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL022C
SGDiS000001505 CDC16

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi44S → A: Abolishes phosphorylation; when associated with A-59; A-95; A-103; A-115 and A-406. 1 Publication1
Mutagenesisi59S → A: Abolishes phosphorylation; when associated with A-44; A-95; A-103; A-115 and A-406. 1 Publication1
Mutagenesisi95S → A: Abolishes phosphorylation; when associated with A-44; A-59; A-103; A-115 and A-406. 1 Publication1
Mutagenesisi103S → A: Abolishes phosphorylation; when associated with A-44; A-59; A-95; A-115 and A-406. 1 Publication1
Mutagenesisi115T → A: Abolishes phosphorylation; when associated with A-44; A-59; A-95; A-103 and A-406. 1 Publication1
Mutagenesisi406T → A: Abolishes phosphorylation; when associated with A-44; A-59; A-95; A-103 and A-115. 1 Publication1
Mutagenesisi482C → Y in CDC16-264; G2/M cell cycle arrest at 36 degrees Celsius. 1 Publication1
Mutagenesisi530S → P in CDC16-183; G2/M cell cycle arrest at 37 degrees Celsius. 1 Publication1
Mutagenesisi557S → F in CDC16-1; G2/M cell cycle arrest at 36 degrees Celsius. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001062691 – 840Anaphase-promoting complex subunit CDC16Add BLAST840

Post-translational modificationi

Phosphorylated by CDC28, which is required for the early mitotic activity of the APC/C in its CDC20-bound form.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP09798
PaxDbiP09798
PRIDEiP09798
TopDownProteomicsiP09798

PTM databases

iPTMnetiP09798

Interactioni

Subunit structurei

The APC/C is composed of at least 13 subunits that stay tightly associated throughout the cell cycle: APC1, APC2, APC4, APC5, APC9, APC11, CDC16, CDC23, CDC26, CDC27, DOC1, MND2 and SWM1. Interacts with AMA1.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34109, 165 interactors
DIPiDIP-25N
IntActiP09798, 34 interactors
MINTiP09798
STRINGi4932.YKL022C

Structurei

3D structure databases

ProteinModelPortaliP09798
SMRiP09798
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati229 – 260TPR 1Add BLAST32
Repeati263 – 288TPR 2Add BLAST26
Repeati296 – 319TPR 3Add BLAST24
Repeati357 – 388TPR 4Add BLAST32
Repeati393 – 416TPR 5Add BLAST24
Repeati426 – 454TPR 6Add BLAST29
Repeati464 – 492TPR 7Add BLAST29
Repeati499 – 526TPR 8Add BLAST28
Repeati531 – 560TPR 9Add BLAST30
Repeati565 – 593TPR 10Add BLAST29
Repeati600 – 628TPR 11Add BLAST29
Repeati633 – 665TPR 12Add BLAST33
Repeati671 – 703TPR 13Add BLAST33
Repeati708 – 737TPR 14Add BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi209 – 220Poly-ThrAdd BLAST12

Sequence similaritiesi

Belongs to the APC6/CDC16 family.Curated

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

GeneTreeiENSGT00510000047327
HOGENOMiHOG000160791
InParanoidiP09798
KOiK03353
OrthoDBiEOG092C0SLF

Family and domain databases

InterProiView protein in InterPro
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR013105 TPR_2
IPR019734 TPR_repeat
PfamiView protein in Pfam
PF07719 TPR_2, 1 hit
PF13181 TPR_8, 1 hit
SMARTiView protein in SMART
SM00028 TPR, 8 hits
SUPFAMiSSF48452 SSF48452, 4 hits
PROSITEiView protein in PROSITE
PS50005 TPR, 7 hits
PS50293 TPR_REGION, 2 hits

Sequencei

Sequence statusi: Complete.

P09798-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFCLYCCHC YIVICGKATH YYKSSKATSN LKSSNRVLMR NPMSPSEQHS
60 70 80 90 100
QHNSTLAASP FVSNVSAART QQSLPTDAQN DRLQQPWNRT NTATSPYQSL
110 120 130 140 150
ANSPLIQKLQ ANIMTPHQPS ANSNSNSNSI TGNVVNDNNL LASMSKNSMF
160 170 180 190 200
GSTIPSTLRK VSLQREYKDS VDGVVRDEDN DEDVHNNGDA AANANNDRES
210 220 230 240 250
KLGHNGPLTT TTLTTTTTAT QLDVSELSAI ERLRLWRFDA LMQHMYRTAE
260 270 280 290 300
YIADKVYNIS NDPDDAFWLG QVYYNNNQYV RAVELITRNN LDGVNILCRY
310 320 330 340 350
LLGLSFVKLQ RFDDALDVIG EYNPFSEDPS TTAANTMSNN GNNSNTSQPV
360 370 380 390 400
TDGGIKMESS LCFLRGKIYF AQNNFNKARD AFREAILVDI KNFEAFEMLL
410 420 430 440 450
SKNLLTPQEE WDLFDSLDFK EFGEDKEIMK NLYKINLSKY INTEDITKSN
460 470 480 490 500
EILAKDYKLA DNVDVVRSKV DICYTQCKFN ECLELCETVL ENDEFNTNIL
510 520 530 540 550
PAYIGCLYEL SNKNKLFLLS HRLAETFPKS AITWFSVATY YMSLDRISEA
560 570 580 590 600
QKYYSKSSIL DPSFAAAWLG FAHTYALEGE QDQALTAYST ASRFFPGMHL
610 620 630 640 650
PKLFLGMQFM AMNSLNLAES YFVLAYDICP NDPLVLNEMG VMYFKKNEFV
660 670 680 690 700
KAKKYLKKAL EVVKDLDPSS RTTISIQLNL GHTYRKLNEN EIAIKCFRCV
710 720 730 740 750
LEKNDKNSEI HCSLGYLYLK TKKLQKAIDH LHKSLYLKPN NSSATALLKN
760 770 780 790 800
ALELNVTLSL DASHPLIDKS NLMSQASKDK ASLNKKRSSL TYDPVNMAKR
810 820 830 840
LRTQKEIFDQ NNKALRKGGH DSKTGSNNAD DDFDADMELE
Length:840
Mass (Da):94,992
Last modified:July 1, 1989 - v1
Checksum:iA096B34441083488
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06165 Genomic DNA Translation: CAA29521.1
Z28022 Genomic DNA Translation: CAA81857.1
BK006944 Genomic DNA Translation: DAA09133.1
PIRiA27832
RefSeqiNP_012903.1, NM_001179588.1

Genome annotation databases

EnsemblFungiiYKL022C; YKL022C; YKL022C
GeneIDi853846
KEGGisce:YKL022C

Similar proteinsi

Entry informationi

Entry nameiCDC16_YEAST
AccessioniPrimary (citable) accession number: P09798
Secondary accession number(s): D6VXR3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: March 28, 2018
This is version 180 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health