Reviewed, UniProtKB/Swiss-Prot P09796 (T2S1_SALIN)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme SinI
      Short name=R.SinI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme SinI
    Endonuclease SinI

Gene names

Name:

sinIR

Organism

Salmonella infantis

Taxonomic identifier

595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded sequence GGWCC and cleaves after G-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 230	230	Type-2 restriction enzyme SinI		PRO_0000077361

Sequences

	Sequence		Length	Mass (Da)
	P09796-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 95BAD4E421E72CAF	FASTA	230	26,870
	10 20 30 40 50 60 MSEAVFFVEN AEELAKQKMD NINPELSEKF QLLIKFLSRF PESCSNPRSK QVRKNFGKAE 70 80 90 100 110 120 HIEYLAQNFN ESRLPKKPTP PTTIPDEVVS LVLNVSFDIP QENLNRIKEE HRLSMASENI 130 140 150 160 170 180 VGDLLERYLA EKLEPCGWIW CSGTSVKAVD FIHYDNEKDE WGLLQVKNRD NTENSSSSKI 190 200 210 220 230 RDNTPIKKWF RTFSQRDATN WENFPDEVSS KDLNEDDFRA FVESYLRKIK

References

[1]	"Cloning and complete nucleotide sequences of the type II restriction-modification genes of Salmonella infantis." Karreman C., de Waard A. J. Bacteriol. 170:2527-2532(1988) [PubMed: 2836359] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	J03391 Genomic DNA. Translation: AAA27213.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1687. SinI.
Enzyme and pathway databases
BRENDA	3.1.21.4. 262474.
Family and domain databases
InterPro	IPR019070. Restrict_endonuc_II_SinI. [Graphical view]
Pfam	PF09570. RE_SinI. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2S1_SALIN

Accession

Primary (citable) accession number: P09796

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	November 3, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)