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P09796 (T2S1_SALIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme SinI

Short name=R.SinI
EC=3.1.21.4
Alternative name(s):
Endonuclease SinI
Type II restriction enzyme SinI
Gene names
Name:sinIR
OrganismSalmonella infantis
Taxonomic identifier595 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length230 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence GGWCC and cleaves after G-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 230230Type-2 restriction enzyme SinI
PRO_0000077361

Sequences

Sequence LengthMass (Da)Tools
P09796 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 95BAD4E421E72CAF

FASTA23026,870
        10         20         30         40         50         60 
MSEAVFFVEN AEELAKQKMD NINPELSEKF QLLIKFLSRF PESCSNPRSK QVRKNFGKAE 

        70         80         90        100        110        120 
HIEYLAQNFN ESRLPKKPTP PTTIPDEVVS LVLNVSFDIP QENLNRIKEE HRLSMASENI 

       130        140        150        160        170        180 
VGDLLERYLA EKLEPCGWIW CSGTSVKAVD FIHYDNEKDE WGLLQVKNRD NTENSSSSKI 

       190        200        210        220        230 
RDNTPIKKWF RTFSQRDATN WENFPDEVSS KDLNEDDFRA FVESYLRKIK 

« Hide

References

[1]"Cloning and complete nucleotide sequences of the type II restriction-modification genes of Salmonella infantis."
Karreman C., de Waard A.
J. Bacteriol. 170:2527-2532(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J03391 Genomic DNA. Translation: AAA27213.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1687. SinI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019070. Restrct_endonuc_II_SinI.
[Graphical view]
PfamPF09570. RE_SinI. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2S1_SALIN
AccessionPrimary (citable) accession number: P09796
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries