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Protein

Modification methylase SinI

Gene

sinIM

Organism
Salmonella infantis
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGWCC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the SinI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei154PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi3504 M.SinI

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase SinI (EC:2.1.1.37)
Short name:
M.SinI
Alternative name(s):
Cytosine-specific methyltransferase SinI
Gene namesi
Name:sinIM
OrganismiSalmonella infantis
Taxonomic identifieri595 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000879051 – 461Modification methylase SinIAdd BLAST461

Structurei

3D structure databases

ProteinModelPortaliP09795
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini75 – 435SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST361

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Family and domain databases

InterProiView protein in InterPro
IPR018117 C5_DNA_meth_AS
IPR001525 C5_MeTfrase
IPR031303 C5_meth_CS
IPR029063 SAM-dependent_MTases
IPR010093 SinI_DNA-bd
PfamiView protein in Pfam
PF00145 DNA_methylase, 1 hit
PRINTSiPR00105 C5METTRFRASE
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00675 dcm, 1 hit
TIGR01764 excise, 1 hit
PROSITEiView protein in PROSITE
PS00094 C5_MTASE_1, 1 hit
PS00095 C5_MTASE_2, 1 hit
PS51679 SAM_MT_C5, 1 hit

Sequencei

Sequence statusi: Complete.

P09795-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIMNDIITVT EAAQLLELTP QRVRTMCKQG SIDAYQSGRT WLIKSSSVEK
60 70 80 90 100
LMLVNSLSDA QNSYSMLASE PKNKPKALSF FSGAMGLDLG IEQAGFETLL
110 120 130 140 150
ASEIDKAARD TILSNRPNMA LIGDIRDYTT EDILKLAGVS SGNEIDLIMG
160 170 180 190 200
GPPCQAFSTA GKRLGLEDER GNVFIKYLDV ALDIRPKYIV IENVRGLLSA
210 220 230 240 250
PMKHRPHNER GEGLPPLKSE EQPGGVLHYI IRIIKSAGYS VSFNLYNSAN
260 270 280 290 300
FGVPQIRERV IIICSRDGSR VPFLQPTHSE KGEYGLPKWI TLRETITNLK
310 320 330 340 350
NITHEHVLFP EKRLKYYRLL KEGQYWKHLP EDLQKEALGK SFFLGGGKTG
360 370 380 390 400
FLRRVAWDRP SPTLVTHPAM PATDLAHPDE LRPLSVQEYK VIQQFPEEWV
410 420 430 440 450
IKGKLLDKYR QLGNAVPIGL GLAVGKNILD HMNGRKIESF PNFRYSRYKN
460
TSDLDIFGEL V
Length:461
Mass (Da):51,767
Last modified:July 1, 1989 - v1
Checksum:i5EA688C4F465BBC4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03391 Genomic DNA Translation: AAA27212.1

Similar proteinsi

Entry informationi

Entry nameiMTS1_SALIN
AccessioniPrimary (citable) accession number: P09795
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: March 28, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health