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P09795 (MTS1_SALIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase SinI

Short name=M.SinI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase SinI
Gene names
Name:sinIM
OrganismSalmonella infantis
Taxonomic identifier595 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length461 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GGWCC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the SinI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 461461Modification methylase SinI
PRO_0000087905

Regions

Domain75 – 435361SAM-dependent MTase C5-type

Sites

Active site1541 By similarity

Sequences

Sequence LengthMass (Da)Tools
P09795 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 5EA688C4F465BBC4

FASTA46151,767
        10         20         30         40         50         60 
MIMNDIITVT EAAQLLELTP QRVRTMCKQG SIDAYQSGRT WLIKSSSVEK LMLVNSLSDA 

        70         80         90        100        110        120 
QNSYSMLASE PKNKPKALSF FSGAMGLDLG IEQAGFETLL ASEIDKAARD TILSNRPNMA 

       130        140        150        160        170        180 
LIGDIRDYTT EDILKLAGVS SGNEIDLIMG GPPCQAFSTA GKRLGLEDER GNVFIKYLDV 

       190        200        210        220        230        240 
ALDIRPKYIV IENVRGLLSA PMKHRPHNER GEGLPPLKSE EQPGGVLHYI IRIIKSAGYS 

       250        260        270        280        290        300 
VSFNLYNSAN FGVPQIRERV IIICSRDGSR VPFLQPTHSE KGEYGLPKWI TLRETITNLK 

       310        320        330        340        350        360 
NITHEHVLFP EKRLKYYRLL KEGQYWKHLP EDLQKEALGK SFFLGGGKTG FLRRVAWDRP 

       370        380        390        400        410        420 
SPTLVTHPAM PATDLAHPDE LRPLSVQEYK VIQQFPEEWV IKGKLLDKYR QLGNAVPIGL 

       430        440        450        460 
GLAVGKNILD HMNGRKIESF PNFRYSRYKN TSDLDIFGEL V 

« Hide

References

[1]"Cloning and complete nucleotide sequences of the type II restriction-modification genes of Salmonella infantis."
Karreman C., de Waard A.
J. Bacteriol. 170:2527-2532(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J03391 Genomic DNA. Translation: AAA27212.1.

3D structure databases

ProteinModelPortalP09795.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3504. M.SinI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR010093. Xis_DNA-bd.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
TIGR01764. excise. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTS1_SALIN
AccessionPrimary (citable) accession number: P09795
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries