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Protein

Modification methylase SinI

Gene

sinIM

Organism
Salmonella infantis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGWCC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the SinI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei154 – 1541PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3504. M.SinI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase SinI (EC:2.1.1.37)
Short name:
M.SinI
Alternative name(s):
Cytosine-specific methyltransferase SinI
Gene namesi
Name:sinIM
OrganismiSalmonella infantis
Taxonomic identifieri595 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 461461Modification methylase SinIPRO_0000087905Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP09795.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini75 – 435361SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
IPR010093. SinI_DNA-bd.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
TIGR01764. excise. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09795-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIMNDIITVT EAAQLLELTP QRVRTMCKQG SIDAYQSGRT WLIKSSSVEK
60 70 80 90 100
LMLVNSLSDA QNSYSMLASE PKNKPKALSF FSGAMGLDLG IEQAGFETLL
110 120 130 140 150
ASEIDKAARD TILSNRPNMA LIGDIRDYTT EDILKLAGVS SGNEIDLIMG
160 170 180 190 200
GPPCQAFSTA GKRLGLEDER GNVFIKYLDV ALDIRPKYIV IENVRGLLSA
210 220 230 240 250
PMKHRPHNER GEGLPPLKSE EQPGGVLHYI IRIIKSAGYS VSFNLYNSAN
260 270 280 290 300
FGVPQIRERV IIICSRDGSR VPFLQPTHSE KGEYGLPKWI TLRETITNLK
310 320 330 340 350
NITHEHVLFP EKRLKYYRLL KEGQYWKHLP EDLQKEALGK SFFLGGGKTG
360 370 380 390 400
FLRRVAWDRP SPTLVTHPAM PATDLAHPDE LRPLSVQEYK VIQQFPEEWV
410 420 430 440 450
IKGKLLDKYR QLGNAVPIGL GLAVGKNILD HMNGRKIESF PNFRYSRYKN
460
TSDLDIFGEL V
Length:461
Mass (Da):51,767
Last modified:July 1, 1989 - v1
Checksum:i5EA688C4F465BBC4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03391 Genomic DNA. Translation: AAA27212.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03391 Genomic DNA. Translation: AAA27212.1.

3D structure databases

ProteinModelPortaliP09795.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3504. M.SinI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
IPR010093. SinI_DNA-bd.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
TIGR01764. excise. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and complete nucleotide sequences of the type II restriction-modification genes of Salmonella infantis."
    Karreman C., de Waard A.
    J. Bacteriol. 170:2527-2532(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiMTS1_SALIN
AccessioniPrimary (citable) accession number: P09795
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: January 7, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.