Reviewed, UniProtKB/Swiss-Prot P09795 (MTS1_SALIN)

Names and origin

Protein names

Recommended name:
    Modification methylase SinI
      Short name=M.SinI
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase SinI

Gene names

Name:

sinIM

Organism

Salmonella infantis

Taxonomic identifier

595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GGWCC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the SinI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Modification methylase SinI		PRO_0000087905
Sites
	Active site	154	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P09795-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 5EA688C4F465BBC4	FASTA	461	51,767
	10 20 30 40 50 60 MIMNDIITVT EAAQLLELTP QRVRTMCKQG SIDAYQSGRT WLIKSSSVEK LMLVNSLSDA 70 80 90 100 110 120 QNSYSMLASE PKNKPKALSF FSGAMGLDLG IEQAGFETLL ASEIDKAARD TILSNRPNMA 130 140 150 160 170 180 LIGDIRDYTT EDILKLAGVS SGNEIDLIMG GPPCQAFSTA GKRLGLEDER GNVFIKYLDV 190 200 210 220 230 240 ALDIRPKYIV IENVRGLLSA PMKHRPHNER GEGLPPLKSE EQPGGVLHYI IRIIKSAGYS 250 260 270 280 290 300 VSFNLYNSAN FGVPQIRERV IIICSRDGSR VPFLQPTHSE KGEYGLPKWI TLRETITNLK 310 320 330 340 350 360 NITHEHVLFP EKRLKYYRLL KEGQYWKHLP EDLQKEALGK SFFLGGGKTG FLRRVAWDRP 370 380 390 400 410 420 SPTLVTHPAM PATDLAHPDE LRPLSVQEYK VIQQFPEEWV IKGKLLDKYR QLGNAVPIGL 430 440 450 460 GLAVGKNILD HMNGRKIESF PNFRYSRYKN TSDLDIFGEL V

References

[1]	"Cloning and complete nucleotide sequences of the type II restriction-modification genes of Salmonella infantis." Karreman C., de Waard A. J. Bacteriol. 170:2527-2532(1988) [PubMed: 2836359] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	J03391 Genomic DNA. Translation: AAA27212.1.
3D structure databases
SMR	P09795. Positions 76-432.
ModBase	Search...
Protein family/group databases
REBASE	3504. M.SinI.
Enzyme and pathway databases
BRENDA	2.1.1.37. 262474.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. IPR010093. Xis_DNA_bd. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit. TIGR01764. excise. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTS1_SALIN

Accession

Primary (citable) accession number: P09795

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	February 9, 2010
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)