Ctla4

Functionⁱ

Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28 (By similarity).By similarity

GO - Biological processⁱ

adaptive immune response Source: UniProtKB-KW
B cell receptor signaling pathway Source: MGI
cellular response to DNA damage stimulus Source: MGI
negative regulation of B cell proliferation Source: MGI
negative regulation of regulatory T cell differentiation Source: MGI
positive regulation of apoptotic process Source: MGI

Complete GO annotation...

Keywordsⁱ

Biological process	Adaptive immunity, Immunity

Biological process

Adaptive immunity, Immunity

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Cytotoxic T-lymphocyte protein 4 Alternative name(s): Cytotoxic T-lymphocyte-associated antigen 4 Short name: CTLA-4 CD_antigen: CD152
Gene namesⁱ	Name:Ctla4 Synonyms:Cd152
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Unplaced

Organism-specific databases

MGIⁱ	MGI:88556. Ctla4.

Subcellular locationⁱ

Cell membrane By similarity; Single-pass type I membrane protein By similarity

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	36 – 161	ExtracellularSequence analysisAdd BLAST	126
Transmembraneⁱ	162 – 182	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	183 – 223	CytoplasmicSequence analysisAdd BLAST	41

GO - Cellular componentⁱ

clathrin-coated endocytic vesicle Source: MGI
external side of plasma membrane
Source: MGI
Inferred from direct assayⁱ
- 16227984
Golgi apparatus Source: MGI
integral component of membrane Source: UniProtKB-KW
perinuclear region of cytoplasm Source: MGI
protein complex involved in cell adhesion
Source: MGI
Inferred from sequence orthologyⁱ
- 7544393

Complete GO annotation...

Keywords - Cellular componentⁱ

Cell membrane, Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 35	Sequence analysisAdd BLAST		35
ChainⁱPRO_0000014735	36 – 223	Cytotoxic T-lymphocyte protein 4Add BLAST		188

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	58 ↔ 129	Combined sources1 Publication
Disulfide bondⁱ	85 ↔ 103	Combined sources1 Publication
Glycosylationⁱ	108	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	113	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	145	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	157	InterchainPROSITE-ProRule annotation
Modified residueⁱ	201	Phosphotyrosine; by TXK and JAK2By similarity	1

Post-translational modificationⁱ

N-glycosylation is important for dimerization.By similarity

Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface.By similarity

Keywords - PTMⁱ

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDⁱ	P09793.
PaxDbⁱ	P09793.
PRIDEⁱ	P09793.

PTM databases

iPTMnetⁱ	P09793.
PhosphoSitePlusⁱ	P09793.

Expressionⁱ

Tissue specificityⁱ

Widely expressed with highest levels in lymphoid tissues.1 Publication

Gene expression databases

CleanExⁱ	MM_CTLA4.

CleanExⁱ

MM_CTLA4.

Interactionⁱ

Subunit structureⁱ

Homodimer; disulfide-linked. Binds to CD80/B7-1 and CD86/B7.2 (By similarity).By similarity

Protein-protein interaction databases

BioGridⁱ	198575. 1 interactor.
IntActⁱ	P09793. 1 interactor.
Molecular INTeraction database More...MINTⁱ	MINT-8019867.
STRINGⁱ	10090.ENSMUSP00000027164.

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	39 – 41	Combined sources	3
Beta strandⁱ	44 – 47	Combined sources	4
Beta strandⁱ	54 – 62	Combined sources	9
Beta strandⁱ	68 – 79	Combined sources	12
Beta strandⁱ	81 – 89	Combined sources	9
Beta strandⁱ	91 – 96	Combined sources	6
Beta strandⁱ	103 – 108	Combined sources	6
Beta strandⁱ	111 – 116	Combined sources	6
Helixⁱ	121 – 123	Combined sources	3
Beta strandⁱ	125 – 138	Combined sources	14
Beta strandⁱ	140 – 143	Combined sources	4
Beta strandⁱ	147 – 150	Combined sources	4

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1DQT	X-ray	2.00	A/B/C/D	38-154	[»]
	5E56	X-ray	1.50	A	38-154	[»]
	5E5M	X-ray	2.18	A/C/E/G	38-154	[»]
ProteinModelPortalⁱ	P09793.
SMRⁱ	P09793.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P09793.

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	36 – 145	Ig-like V-typeAdd BLAST		110

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	46 – 50	HomodimerizationBy similarity		5
Regionⁱ	150 – 155	HomodimerizationBy similarity		6

Keywords - Domainⁱ

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG410IJ05. Eukaryota. ENOG410YUQR. LUCA.
HOGENOMⁱ	HOG000112047.
HOVERGENⁱ	HBG057978.
InParanoidⁱ	P09793.
KEGG Orthology (KO) More...KOⁱ	K06538.
PhylomeDBⁱ	P09793.

Family and domain databases

Gene3Dⁱ	2.60.40.10. 1 hit.
InterProⁱ	View protein in InterPro IPR008096. CTLA4. IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR013106. Ig_V-set.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07686. V-set. 1 hit.
PRINTSⁱ	PR01720. CTLANTIGEN4.
SMARTⁱ	View protein in SMART SM00409. IG. 1 hit. SM00406. IGv. 1 hit.
SUPFAMⁱ	SSF48726. SSF48726. 1 hit.
PROSITEⁱ	View protein in PROSITE PS50835. IG_LIKE. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P09793-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MACLGLRRYK AQLQLPSRTW PFVALLTLLF IPVFSEAIQV TQPSVVLASS 
        60         70         80         90        100
HGVASFPCEY SPSHNTDEVR VTVLRQTNDQ MTEVCATTFT EKNTVGFLDY 
       110        120        130        140        150
PFCSGTFNES RVNLTIQGLR AVDTGLYLCK VELMYPPPYF VGMGNGTQIY 
       160        170        180        190        200
VIDPEPCPDS DFLLWILVAV SLGLFFYSFL VSAVSLSKML KKRSPLTTGV 
       210        220 
YVKMPPTEPE CEKQFQPYFI PIN

Length:223

Mass (Da):24,993

Last modified:July 1, 1989 - v1

Checksum:ⁱ5318FAAF416F4685

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	182	S → T in AAF01489 (PubMed:10493833).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X05719 mRNA. Translation: CAA29191.1. AF142145 Genomic DNA. Translation: AAF01489.1. M74362 Genomic DNA. Translation: AAA37489.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS14993.1.
PIRⁱ	A29063.
NCBI Reference Sequences More...RefSeqⁱ	NP_033973.2. NM_009843.4.
UniGeneⁱ	Mm.390.

Genome annotation databases

GeneIDⁱ	12477.
KEGGⁱ	mmu:12477.

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X05719 mRNA. Translation: CAA29191.1. AF142145 Genomic DNA. Translation: AAF01489.1. M74362 Genomic DNA. Translation: AAA37489.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS14993.1.
PIRⁱ	A29063.
NCBI Reference Sequences More...RefSeqⁱ	NP_033973.2. NM_009843.4.
UniGeneⁱ	Mm.390.

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1DQT	X-ray	2.00	A/B/C/D	38-154	[»]
	5E56	X-ray	1.50	A	38-154	[»]
	5E5M	X-ray	2.18	A/C/E/G	38-154	[»]
ProteinModelPortalⁱ	P09793.
SMRⁱ	P09793.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	198575. 1 interactor.
IntActⁱ	P09793. 1 interactor.
Molecular INTeraction database More...MINTⁱ	MINT-8019867.
STRINGⁱ	10090.ENSMUSP00000027164.

PTM databases

iPTMnetⁱ	P09793.
PhosphoSitePlusⁱ	P09793.

Proteomic databases

EPDⁱ	P09793.
PaxDbⁱ	P09793.
PRIDEⁱ	P09793.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	12477.
KEGGⁱ	mmu:12477.

Organism-specific databases

CTDⁱ	1493.
MGIⁱ	MGI:88556. Ctla4.

Phylogenomic databases

eggNOGⁱ	ENOG410IJ05. Eukaryota. ENOG410YUQR. LUCA.
HOGENOMⁱ	HOG000112047.
HOVERGENⁱ	HBG057978.
InParanoidⁱ	P09793.
KEGG Orthology (KO) More...KOⁱ	K06538.
PhylomeDBⁱ	P09793.

Miscellaneous databases

EvolutionaryTraceⁱ	P09793.
Protein Ontology More...PROⁱ	PR:P09793.
SOURCEⁱ	Search...

Gene expression databases

CleanExⁱ	MM_CTLA4.

CleanExⁱ

MM_CTLA4.

Family and domain databases

Gene3Dⁱ	2.60.40.10. 1 hit.
InterProⁱ	View protein in InterPro IPR008096. CTLA4. IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR013106. Ig_V-set.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07686. V-set. 1 hit.
PRINTSⁱ	PR01720. CTLANTIGEN4.
SMARTⁱ	View protein in SMART SM00409. IG. 1 hit. SM00406. IGv. 1 hit.
SUPFAMⁱ	SSF48726. SSF48726. 1 hit.
PROSITEⁱ	View protein in PROSITE PS50835. IG_LIKE. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	CTLA4_MOUSE
Accessionⁱ	P09793Primary (citable) accession number: P09793 Secondary accession number(s): Q9QZZ7
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
	Last sequence update:	July 1, 1989
	Last modified:	May 10, 2017
	This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P09793 (CTLA4_MOUSE)

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Cytotoxic T-lymphocyte protein 4

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ