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P09793 (CTLA4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytotoxic T-lymphocyte protein 4
Alternative name(s):
Cytotoxic T-lymphocyte-associated antigen 4
Short name=CTLA-4
CD_antigen=CD152
Gene names
Name:Ctla4
Synonyms:Cd152
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length223 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28 By similarity.

Subunit structure

Homodimer; disulfide-linked. Binds to CD80/B7-1 and CD86/B7.2 By similarity.

Subcellular location

Cell membrane; Single-pass type I membrane protein By similarity.

Tissue specificity

Widely expressed with highest levels in lymphoid tissues. Ref.2

Post-translational modification

N-glycosylation is important for dimerization By similarity.

Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface By similarity.

Sequence similarities

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3535 Potential
Chain36 – 223188Cytotoxic T-lymphocyte protein 4
PRO_0000014735

Regions

Topological domain36 – 161126Extracellular Potential
Transmembrane162 – 18221Helical; Potential
Topological domain183 – 22341Cytoplasmic Potential
Domain36 – 145110Ig-like V-type
Region46 – 505Homodimerization By similarity
Region150 – 1556Homodimerization By similarity

Amino acid modifications

Modified residue2011Phosphotyrosine; by TXK and JAK2 By similarity
Glycosylation1081N-linked (GlcNAc...) Potential
Glycosylation1131N-linked (GlcNAc...) Potential
Glycosylation1451N-linked (GlcNAc...) Potential
Disulfide bond58 ↔ 129
Disulfide bond85 ↔ 103
Disulfide bond157Interchain By similarity

Experimental info

Sequence conflict1821S → T in AAF01489. Ref.2

Secondary structure

......................... 223
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P09793 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 5318FAAF416F4685

FASTA22324,993
        10         20         30         40         50         60 
MACLGLRRYK AQLQLPSRTW PFVALLTLLF IPVFSEAIQV TQPSVVLASS HGVASFPCEY 

        70         80         90        100        110        120 
SPSHNTDEVR VTVLRQTNDQ MTEVCATTFT EKNTVGFLDY PFCSGTFNES RVNLTIQGLR 

       130        140        150        160        170        180 
AVDTGLYLCK VELMYPPPYF VGMGNGTQIY VIDPEPCPDS DFLLWILVAV SLGLFFYSFL 

       190        200        210        220 
VSAVSLSKML KKRSPLTTGV YVKMPPTEPE CEKQFQPYFI PIN 

« Hide

References

[1]"A new member of the immunoglobulin superfamily -- CTLA-4."
Brunet J.-F., Denizot F., Luciani M.-F., Roux-Dosseto M., Suzan M., Mattei M.-G., Golstein P.
Nature 328:267-270(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Complete sequence determination of the mouse and human CTLA4 gene loci: cross-species DNA sequence similarity beyond exon borders."
Ling V., Wu P.W., Finnerty H.F., Sharpe A.H., Gray G.S., Collins M.
Genomics 60:341-355(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
Strain: 129/SvJ.
[3]"CTLA-4 and CD28 activated lymphocyte molecules are closely related in both mouse and human as to sequence, message expression, gene structure, and chromosomal location."
Harper K., Balzano C., Rouvier E., Mattei M.-G., Luciani M.-F., Golstein P.
J. Immunol. 147:1037-1044(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 1-36.
[4]"Structure of murine CTLA-4 and its role in modulating T cell responsiveness."
Ostrov D.A., Shi W., Schwartz J.-C., Almo S.C., Nathenson S.G.
Science 290:816-819(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 38-154.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X05719 mRNA. Translation: CAA29191.1.
AF142145 Genomic DNA. Translation: AAF01489.1.
M74362 Genomic DNA. Translation: AAA37489.1.
CCDSCCDS14993.1.
PIRA29063.
RefSeqNP_033973.2. NM_009843.4.
UniGeneMm.390.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1DQTX-ray2.00A/B/C/D38-154[»]
ProteinModelPortalP09793.
SMRP09793. Positions 38-154.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198575. 1 interaction.
IntActP09793. 1 interaction.
MINTMINT-8019867.

PTM databases

PhosphoSiteP09793.

Proteomic databases

PRIDEP09793.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID12477.
KEGGmmu:12477.

Organism-specific databases

CTD1493.
MGIMGI:88556. Ctla4.

Phylogenomic databases

eggNOGNOG42442.
HOGENOMHOG000112047.
HOVERGENHBG057978.
InParanoidP09793.
KOK06538.
PhylomeDBP09793.

Gene expression databases

CleanExMM_CTLA4.
GenevestigatorP09793.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
InterProIPR008096. CTLA4.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamPF07686. V-set. 1 hit.
[Graphical view]
PRINTSPR01720. CTLANTIGEN4.
SMARTSM00409. IG. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP09793.
NextBio281362.
PROP09793.
SOURCESearch...

Entry information

Entry nameCTLA4_MOUSE
AccessionPrimary (citable) accession number: P09793
Secondary accession number(s): Q9QZZ7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: July 9, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot