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P09792 (GST28_SCHMA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione S-transferase class-mu 28 kDa isozyme

Short name=GST 28
EC=2.5.1.18
Alternative name(s):
GSH transferase S.m. 1-1
Protective 28 kDa antigen
Sm28 antigen
Sm28GST
Smp28
Gene names
Name:GST28
ORF Names:Smp_054160
OrganismSchistosoma mansoni (Blood fluke) [Reference proteome]
Taxonomic identifier6183 [NCBI]
Taxonomic lineageEukaryotaMetazoaPlatyhelminthesTrematodaDigeneaStrigeididaSchistosomatoideaSchistosomatidaeSchistosoma

Protein attributes

Sequence length211 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.

Catalytic activity

RX + glutathione = HX + R-S-glutathione.

Subunit structure

Homodimer. Ref.6

Tissue specificity

In the adult, expressed in excretory epithelial cells but absent from the caecal epithelium and flame cells. Also expressed in the tegument and its extensions into the parenchyma. In the schistosomulum, expressed in the tegument and associated structures. Not expressed in digestive tract, reproductive organs or muscles (at protein level). Ref.6

Miscellaneous

There are at least three isoenzymes of GST in S.mansoni.

Sequence similarities

Belongs to the GST superfamily. Mu family.

Contains 1 GST C-terminal domain.

Contains 1 GST N-terminal domain.

Ontologies

Keywords
   Molecular functionTransferase
   PTMAcetylation
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Molecular_functionglutathione transferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 211210Glutathione S-transferase class-mu 28 kDa isozyme
PRO_0000185815

Regions

Domain4 – 8683GST N-terminal
Domain88 – 211124GST C-terminal
Region41 – 455Glutathione binding
Region70 – 712Glutathione binding

Sites

Binding site101Glutathione
Binding site161Glutathione
Binding site531Glutathione; via amide nitrogen and carbonyl oxygen

Amino acid modifications

Modified residue21N-acetylalanine Ref.7

Experimental info

Sequence conflict471T → I AA sequence Ref.6
Sequence conflict1061E → D AA sequence Ref.6

Secondary structure

.................................... 211
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P09792 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: F988AAF041D767E3

FASTA21123,820
        10         20         30         40         50         60 
MAGEHIKVIY FDGRGRAESI RMTLVAAGVD YEDERISFQD WPKIKPTIPG GRLPAVKVTD 

        70         80         90        100        110        120 
DHGHVKWMLE SLAIARYMAK KHHMMGETDE EYYSVEKLIG QAEDVEHEYH KTLMKPQEEK 

       130        140        150        160        170        180 
EKITKEILNG KVPVLLNMIC ESLKGSTGKL AVGDKVTLAD LVLIAVIDHV TDLDKGFLTG 

       190        200        210 
KYPEIHKHRE NLLASSPRLA KYLSNRPATP F 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of a protective antigen of schistosomes."
Balloul J.-M., Sondermeyer P., Dreyer D., Capron M., Grzych J.M., Pierce R.J., Carvallo D., Lecocq J.-P., Capron A.
Nature 326:149-153(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]"Cloning and characterisation of the gene encoding the 28-kDa glutathione S-transferase of Schistosoma mansoni."
McNair A.T., Dissous C., Duvaux-Miret O., Capron A.
Gene 124:245-249(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Schistosoma mansoni: characterization of sequence variants of the 28-kDa glutathione S-transferase."
Pierce R.J., Khalife J., Williams D.L., Kanno R., Trottein F., Lepresle T., Sabatier J., Achstetter T., Capron A.
Exp. Parasitol. 79:81-84(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Puerto Rican.
[4]"The genome of the blood fluke Schistosoma mansoni."
Berriman M., Haas B.J., LoVerde P.T., Wilson R.A., Dillon G.P., Cerqueira G.C., Mashiyama S.T., Al-Lazikani B., Andrade L.F., Ashton P.D., Aslett M.A., Bartholomeu D.C., Blandin G., Caffrey C.R., Coghlan A., Coulson R., Day T.A., Delcher A. expand/collapse author list , DeMarco R., Djikeng A., Eyre T., Gamble J.A., Ghedin E., Gu Y., Hertz-Fowler C., Hirai H., Hirai Y., Houston R., Ivens A., Johnston D.A., Lacerda D., Macedo C.D., McVeigh P., Ning Z., Oliveira G., Overington J.P., Parkhill J., Pertea M., Pierce R.J., Protasio A.V., Quail M.A., Rajandream M.A., Rogers J., Sajid M., Salzberg S.L., Stanke M., Tivey A.R., White O., Williams D.L., Wortman J., Wu W., Zamanian M., Zerlotini A., Fraser-Liggett C.M., Barrell B.G., El-Sayed N.M.
Nature 460:352-358(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"A systematically improved high quality genome and transcriptome of the human blood fluke Schistosoma mansoni."
Protasio A.V., Tsai I.J., Babbage A., Nichol S., Hunt M., Aslett M.A., De Silva N., Velarde G.S., Anderson T.J., Clark R.C., Davidson C., Dillon G.P., Holroyd N.E., LoVerde P.T., Lloyd C., McQuillan J., Oliveira G., Otto T.D. expand/collapse author list , Parker-Manuel S.J., Quail M.A., Wilson R.A., Zerlotini A., Dunne D.W., Berriman M.
PLoS Negl. Trop. Dis. 6:E1455-E1455(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Puerto Rican.
[6]"The glutathione transferase activity and tissue distribution of a cloned Mr28K protective antigen of Schistosoma mansoni."
Taylor J.B., Vidal A., Torpier G., Meyer D.J., Roitsch C., Balloul J.M., Southan C., Sondermeyer P., Pemble S., Lecocq J.P., Capron A., Ketterer B.
EMBO J. 7:465-472(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 5-47; 71-82; 92-106; 127-138; 142-152 AND 191-205, SUBUNIT, TISSUE SPECIFICITY.
[7]"Analysis of the primary structure and post-translational modifications of the Schistosoma mansoni antigen Smp28 by electrospray mass spectrometry."
Bouchon B., Jaquinod M., Klarskov K., Trottein F., Klein M., van Dorsselaer A., Bischoff R., Roitsch C.
J. Chromatogr. B 662:279-290(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY.
[8]"Crystallization and preliminary X-ray diffraction studies of a protective cloned 28 kDa glutathione S-transferase from Schistosoma mansoni."
Trottein F., Vaney M.C., Bachet B., Pierce R.J., Colloc'h N., Lecocq J.-P., Capron A., Mornon J.-P.
J. Mol. Biol. 224:515-518(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.89 ANGSTROMS) IN COMPLEX WITH GLUTATHIONE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X05148 mRNA. Translation: CAA28796.1.
M98271 Genomic DNA. Translation: AAA29891.1.
S71584 mRNA. Translation: AAC60508.1.
CABG01000068 Genomic DNA. Translation: CCD60449.1.
PIRJU0137.
S02458.
RefSeqXP_002576704.1. XM_002576658.1.
UniGeneSma.6813.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1U3IX-ray1.89A1-211[»]
ProteinModelPortalP09792.
SMRP09792. Positions 4-211.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaSmp_054160.1; Smp_054160.1:pep; Smp_054160.
GeneID8342765.
KEGGsmm:Smp_054160.

Organism-specific databases

CTD8342765.

Phylogenomic databases

HOGENOMHOG000115733.
KOK01830.
PhylomeDBP09792.

Family and domain databases

Gene3D1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP09792.

Entry information

Entry nameGST28_SCHMA
AccessionPrimary (citable) accession number: P09792
Secondary accession number(s): G4LZY1, Q7M446
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references