P09786 (PHNB_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anthranilate synthase component II, pyocyanin specific
EC=4.1.3.27

Alternative name(s):
Glutamine amidotransferase

Gene names

Name:	phnB
Ordered Locus Names:	PA1002

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Participates in the biosynthesis of pyocyanin, a characteristic blue-green phenazine pigment produced by P.aeruginosa.
Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Secondary metabolite biosynthesis; pyocyanine biosynthesis.
Subunit structure	Tetramer of two components I and two components II.
Miscellaneous	Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Domain	Glutamine amidotransferase
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	anthranilate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 200	200	Anthranilate synthase component II, pyocyanin specific		PRO_0000056906
Regions
	Domain	2 – 195	194	Glutamine amidotransferase type-1
Sites
	Active site	83	1	By similarity
	Active site	169	1	By similarity
	Active site	171	1	By similarity
Experimental info
	Sequence conflict	148 – 149	2	DA → ES in AAA26018. Ref.2
	Sequence conflict	195	1	R → A in AAA88449. Ref.1
	Sequence conflict	195	1	R → A in AAA88452. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P09786 [UniParc]. Last modified December 8, 2000. Version 3. Checksum: 9D9E218DDE8D7E30	FASTA	200	21,845
	10 20 30 40 50 60 MRITLLDNFD SFTYNLVEQF CLLGAEVRVM RNDTPLPTIQ AALLADGCEL LVLSPGPGRP 70 80 90 100 110 120 EDAGCMLELL AWARGRLPVL GVCLGHQALA LAAGGAVGEA RKPLHGKSTS LRFDQRHPLF 130 140 150 160 170 180 DGIADLRVAR YHSLVVSRLP EGFDCLADAD GEIMAMADPR NRQLGLQFHP ESILTTHGQR 190 200 LLENALLWCG ALAVRERLRA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: interchangeability of the two anthranilate synthases and evolutionary implications." Essar D.W., Eberly L., Hadero A., Crawford I.P. J. Bacteriol. 172:884-900(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228 and PAC174.
[2]	"Structure and regulation of the anthranilate synthase genes in Pseudomonas aeruginosa: I. Sequence of trpG encoding the glutamine amidotransferase subunit." Crawford I.P., Eberly L. Mol. Biol. Evol. 3:436-448(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M15733 Genomic DNA. Translation: AAA26018.1. M33810 Genomic DNA. Translation: AAA88449.1. M33811 Genomic DNA. Translation: AAA88452.1. AE004091 Genomic DNA. Translation: AAG04391.1.
PIR	B35116. C83519.
RefSeq	NP_249693.1. NC_002516.2.
3D structure databases
ProteinModelPortal	P09786.
SMR	P09786. Positions 3-188.
ModBase	Search...
Protein-protein interaction databases
STRING	208964.PA1002.
Protein family/group databases
MEROPS	C26.955.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	880689.
KEGG	pae:PA1002.
PATRIC	19836294. VBIPseAer58763_1034.
Organism-specific databases
PseudoCAP	PA1002.
Phylogenomic databases
eggNOG	COG0512.
HOGENOM	HOG000025029.
KO	K01658.
OMA	YLIGLMN.
ProtClustDB	CLSK866272.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-16008.
UniPathway	UPA00235.
Family and domain databases
InterPro	IPR017926. GATASE. IPR006221. TrpG/PapA_dom. [Graphical view]
Pfam	PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00566. trpG_papA. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHNB_PSEAE

Accession

Primary (citable) accession number: P09786

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	December 8, 2000
Last modified:	May 29, 2013
This is version 94 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program