ID   PHNA_PSEAE              Reviewed;         530 AA.
AC   P09785;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 2.
DT   16-JUN-2009, entry version 71.
DE   RecName: Full=Anthranilate synthase component 1;
DE            EC=4.1.3.27;
DE   AltName: Full=Anthranilate synthase component I;
GN   Name=phnA; OrderedLocusNames=PA1001;
OS   Pseudomonas aeruginosa.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228, and PAC174;
RX   MEDLINE=90130326; PubMed=2153661;
RA   Essar D.W., Eberly L., Hadero A., Crawford I.P.;
RT   "Identification and characterization of genes for a second
RT   anthranilate synthase in Pseudomonas aeruginosa: interchangeability of
RT   the two anthranilate synthases and evolutionary implications.";
RL   J. Bacteriol. 172:884-900(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
RX   MEDLINE=20437337; PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T.,
RA   Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an
RT   opportunistic pathogen.";
RL   Nature 406:959-964(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 383-530.
RX   MEDLINE=88174363; PubMed=3127654;
RA   Crawford I.P., Eberly L.;
RT   "Structure and regulation of the anthranilate synthase genes in
RT   Pseudomonas aeruginosa: I. Sequence of trpG encoding the glutamine
RT   amidotransferase subunit.";
RL   Mol. Biol. Evol. 3:436-448(1986).
CC   -!- FUNCTION: Participates in the biosynthesis of pyocyanin, a
CC       characteristic blue-green phenazine pigment produced by
CC       P.aeruginosa.
CC   -!- CATALYTIC ACTIVITY: Chorismate + L-glutamine = anthranilate +
CC       pyruvate + L-glutamate.
CC   -!- PATHWAY: Secondary metabolite biosynthesis; pyocyanin
CC       biosynthesis.
CC   -!- SUBUNIT: Tetramer of two components I and two components II.
CC   -!- MISCELLANEOUS: Component I catalyzes the formation of anthranilate
CC       using ammonia rather than glutamine, whereas component II provides
CC       glutamine amidotransferase activity.
CC   -!- MISCELLANEOUS: The sequence shown is that of strain PAO.
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DR   EMBL; M33810; AAA88448.1; -; Genomic_DNA.
DR   EMBL; M33811; AAA88451.1; -; Genomic_DNA.
DR   EMBL; AE004091; AAG04390.1; -; Genomic_DNA.
DR   EMBL; M15733; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; B83519; B83519.
DR   PIR; E35116; E35116.
DR   RefSeq; NP_249692.1; -.
DR   HSSP; P00897; 1I7S.
DR   GeneID; 878421; -.
DR   GenomeReviews; AE004091_GR; PA1001.
DR   KEGG; pae:PA1001; -.
DR   NMPDR; fig|208964.1.peg.1002; -.
DR   PseudoCAP; PA1001; -.
DR   HOGENOM; P09785; -.
DR   OMA; P09785; HKELAEH.
DR   BioCyc; PAER208964:PA1001-MON; -.
DR   BRENDA; 4.1.3.27; 354.
DR   GO; GO:0004049; F:anthranilate synthase activity; IEA:EC.
DR   GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR   InterPro; IPR005801; ADC_synthase.
DR   InterPro; IPR006805; Anth_synth_I_N.
DR   InterPro; IPR019999; Anthranilate_synth_I_C.
DR   InterPro; IPR015890; Chorismate-bd_C.
DR   InterPro; IPR005257; TrpE_synth.
DR   Gene3D; G3DSA:3.60.120.10; TRPE_1_chor_bd; 1.
DR   PANTHER; PTHR11236; TRPE_1_chor_bd; 1.
DR   Pfam; PF04715; Anth_synt_I_N; 1.
DR   Pfam; PF00425; Chorismate_bind; 1.
DR   PRINTS; PR00095; ANTSNTHASEI.
DR   ProDom; PD000779; Anth_synth_chor; 1.
DR   TIGRFAMs; TIGR00565; trpE_proteo; 1.
PE   4: Predicted;
KW   Complete proteome; Lyase.
FT   CHAIN         1    530       Anthranilate synthase component 1.
FT                                /FTId=PRO_0000058388.
FT   VARIANT       4      4       R -> G (in strain: PAC174).
FT   VARIANT      91     91       D -> A (in strain: PAC174).
FT   VARIANT     111    111       Q -> P (in strain: PAC174).
FT   VARIANT     114    114       S -> P (in strain: PAC174).
FT   VARIANT     145    145       V -> A (in strain: PAC174).
FT   VARIANT     337    337       R -> C (in strain: PAC174).
SQ   SEQUENCE   530 AA;  58670 MW;  0E6280AC3A23A066 CRC64;
     MGARRWLVSG VGYRLEESLE YRTLVPEALS IWRMAGANRM LFDCFDVDSK AARRSVAILS
     SCLRIECWGR DVVLRALNSN GRALLAPLSE DCPAQVTCLR DGDTLHWRFP QEESHADEWR
     RLHGLSSLEA LRRVLGTLGD AEGPVLLGGL FSFDLAEQFE PLPAPAEPAR HCPDYLFLVP
     ELLLDIDHLA RRTSLQAFVH DPAGHDRLAA SLRQCADEFH GAVEEASESP VAGVRAGNYQ
     VDLDDASFAR QVERLQAHVR AGDVFQIVPS RSFSMPCADP WRAYRQLCLR NPSPYRFFLD
     AGDFCLFGAS PESALKYDAE SREVELYPIA GTRPRGRDAR GAIDAELDNR LEAELRLDAK
     EIAEHMMLVD LARNDLARVC RSGTRQVRDM LKVDRYSHVM HLVSRVAGEL HGELDALHAY
     RACLNMGTLV GAPKVRAMQL LRQYEDGYRG SYGGAIGILD SAGNLDTSIV IRSAEVREGI
     ARVRAGAGVV LDSDPRLEAE ETRNKALAVL TAVAAAERER GERDAHHAVG
//