Reviewed, UniProtKB/Swiss-Prot P09785 (PHNA_PSEAE)

Names and origin

Protein names

Recommended name:
    Anthranilate synthase component 1
    EC=4.1.3.27
Alternative name(s):
    Anthranilate synthase component I

Gene names

Name:	phnA
Ordered Locus Names:	PA1001

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Participates in the biosynthesis of pyocyanin, a characteristic blue-green phenazine pigment produced by P.aeruginosa.
Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Secondary metabolite biosynthesis; pyocyanin biosynthesis.
Subunit structure	Tetramer of two components I and two components II.
Miscellaneous	Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity. The sequence shown is that of strain PAO.

Ontologies

Keywords
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	anthranilate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 530	530	Anthranilate synthase component 1		PRO_0000058388
Natural variations
	Natural variant	4	1	R → G in strain: PAC174.
	Natural variant	91	1	D → A in strain: PAC174.
	Natural variant	111	1	Q → P in strain: PAC174.
	Natural variant	114	1	S → P in strain: PAC174.
	Natural variant	145	1	V → A in strain: PAC174.
	Natural variant	337	1	R → C in strain: PAC174.

Sequences

	Sequence		Length	Mass (Da)
	P09785-1 [UniParc]. Last modified February 1, 1991. Version 2. Checksum: 0E6280AC3A23A066	FASTA	530	58,670
	10 20 30 40 50 60 MGARRWLVSG VGYRLEESLE YRTLVPEALS IWRMAGANRM LFDCFDVDSK AARRSVAILS 70 80 90 100 110 120 SCLRIECWGR DVVLRALNSN GRALLAPLSE DCPAQVTCLR DGDTLHWRFP QEESHADEWR 130 140 150 160 170 180 RLHGLSSLEA LRRVLGTLGD AEGPVLLGGL FSFDLAEQFE PLPAPAEPAR HCPDYLFLVP 190 200 210 220 230 240 ELLLDIDHLA RRTSLQAFVH DPAGHDRLAA SLRQCADEFH GAVEEASESP VAGVRAGNYQ 250 260 270 280 290 300 VDLDDASFAR QVERLQAHVR AGDVFQIVPS RSFSMPCADP WRAYRQLCLR NPSPYRFFLD 310 320 330 340 350 360 AGDFCLFGAS PESALKYDAE SREVELYPIA GTRPRGRDAR GAIDAELDNR LEAELRLDAK 370 380 390 400 410 420 EIAEHMMLVD LARNDLARVC RSGTRQVRDM LKVDRYSHVM HLVSRVAGEL HGELDALHAY 430 440 450 460 470 480 RACLNMGTLV GAPKVRAMQL LRQYEDGYRG SYGGAIGILD SAGNLDTSIV IRSAEVREGI 490 500 510 520 530 ARVRAGAGVV LDSDPRLEAE ETRNKALAVL TAVAAAERER GERDAHHAVG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: interchangeability of the two anthranilate synthases and evolutionary implications." Essar D.W., Eberly L., Hadero A., Crawford I.P. J. Bacteriol. 172:884-900(1990) [PubMed: 2153661] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228 and PAC174.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[3]	"Structure and regulation of the anthranilate synthase genes in Pseudomonas aeruginosa: I. Sequence of trpG encoding the glutamine amidotransferase subunit." Crawford I.P., Eberly L. Mol. Biol. Evol. 3:436-448(1986) [PubMed: 3127654] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 383-530.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M33810 Genomic DNA. Translation: AAA88448.1. M33811 Genomic DNA. Translation: AAA88451.1. AE004091 Genomic DNA. Translation: AAG04390.1. M15733 Genomic DNA. No translation available.
PIR	B83519. E35116.
RefSeq	NP_249692.1.
3D structure databases
HSSP	HSSP built from PDB template 1I7S based on UniProtKB P00897.
ModBase	Search...
Genome annotation databases
GeneID	878421.
GenomeReviews	Gene locus PA1001 in contig AE004091_GR.
KEGG	pae:PA1001.
NMPDR	fig|208964.1.peg.1002.
Organism-specific databases
PseudoCAP	PA1001.
CMR	Search...
Phylogenomic databases
HOGENOM	P09785.
OMA	P09785. HKELAEH.
Enzyme and pathway databases
BioCyc	PAER208964:PA1001-MON.
BRENDA	4.1.3.27. 354.
Family and domain databases
InterPro	IPR005801. ADC_synthase. IPR006805. Anth_synth_I_N. IPR019999. Anthranilate_synth_I_C. IPR015890. Chorismate-bd_C. IPR005257. TrpE_synth. [Graphical view]
Gene3D	G3DSA:3.60.120.10. TRPE_1_chor_bd. 1 hit.
PANTHER	PTHR11236. TRPE_1_chor_bd. 1 hit.
Pfam	PF04715. Anth_synt_I_N. 1 hit. PF00425. Chorismate_bind. 1 hit. [Graphical view]
PRINTS	PR00095. ANTSNTHASEI.
ProDom	PD000779. Anth_synth_chor. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00565. trpE_proteo. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PHNA_PSEAE

Accession

Primary (citable) accession number: P09785

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	February 1, 1991
Last modified:	June 16, 2009
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)