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Protein

Nitrogenase molybdenum-iron protein beta chain

Gene

nifK

Organism
Klebsiella pneumoniae
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.

Catalytic activityi

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.

Cofactori

[8Fe-7S] clusterNote: Binds 1 [8Fe-7S] cluster per heterodimer.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi69Iron-sulfur (8Fe-7S); shared with alpha chain1
Metal bindingi94Iron-sulfur (8Fe-7S); shared with alpha chain1
Metal bindingi152Iron-sulfur (8Fe-7S); shared with alpha chain1
Metal bindingi187Iron-sulfur (8Fe-7S); shared with alpha chain1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processNitrogen fixation
LigandATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:NIFKKLEB-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogenase molybdenum-iron protein beta chain (EC:1.18.6.1)
Alternative name(s):
Dinitrogenase
Nitrogenase component I
Gene namesi
Name:nifK
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001531011 – 520Nitrogenase molybdenum-iron protein beta chainAdd BLAST520

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2).

Protein-protein interaction databases

DIPiDIP-6207N.

Structurei

Secondary structure

1520
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi11 – 14Combined sources4
Helixi18 – 28Combined sources11
Turni29 – 32Combined sources4
Helixi36 – 46Combined sources11
Helixi49 – 55Combined sources7
Beta strandi62 – 64Combined sources3
Helixi70 – 79Combined sources10
Beta strandi84 – 90Combined sources7
Helixi92 – 106Combined sources15
Helixi121 – 124Combined sources4
Helixi127 – 141Combined sources15
Beta strandi144 – 150Combined sources7
Helixi152 – 157Combined sources6
Helixi161 – 170Combined sources10
Helixi192 – 208Combined sources17
Beta strandi217 – 224Combined sources8
Helixi231 – 244Combined sources14
Beta strandi248 – 252Combined sources5
Turni254 – 258Combined sources5
Helixi275 – 280Combined sources6
Helixi281 – 283Combined sources3
Beta strandi284 – 291Combined sources8
Turni292 – 294Combined sources3
Helixi296 – 304Combined sources9
Helixi319 – 333Combined sources15
Helixi339 – 359Combined sources21
Beta strandi363 – 368Combined sources6
Helixi370 – 382Combined sources13
Beta strandi386 – 392Combined sources7
Helixi397 – 409Combined sources13
Beta strandi417 – 421Combined sources5
Helixi424 – 434Combined sources11
Beta strandi437 – 441Combined sources5
Helixi443 – 445Combined sources3
Helixi446 – 455Combined sources10
Helixi457 – 459Combined sources3
Beta strandi463 – 465Combined sources3
Beta strandi472 – 475Combined sources4
Helixi476 – 478Combined sources3
Helixi483 – 505Combined sources23
Turni509 – 511Combined sources3
Helixi513 – 515Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1H1LX-ray1.90B/D2-520[»]
1QGUX-ray1.60B/D2-520[»]
1QH1X-ray1.60B/D2-520[»]
1QH8X-ray1.60B/D2-520[»]
ProteinModelPortaliP09772.
SMRiP09772.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09772.

Family & Domainsi

Sequence similaritiesi

Belongs to the NifD/NifK/NifE/NifN family.Curated

Family and domain databases

InterProiView protein in InterPro
IPR000510. Nase/OxRdtase_comp1.
IPR000318. Nase_comp1_CS.
IPR005976. Nase_Mo-Fe_CF_bsu.
IPR024564. Nase_Mo-Fe_CF_bsu_N.
PfamiView protein in Pfam
PF11844. DUF3364. 1 hit.
PF00148. Oxidored_nitro. 1 hit.
TIGRFAMsiTIGR01286. nifK. 1 hit.
PROSITEiView protein in PROSITE
PS00699. NITROGENASE_1_1. 1 hit.
PS00090. NITROGENASE_1_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P09772-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQTIDKINS CYPLFEQDEY QELFRNKRQL EEAHDAQRVQ EVFAWTTTAE
60 70 80 90 100
YEALNFRREA LTVDPAKACQ PLGAVLCSLG FANTLPYVHG SQGCVAYFRT
110 120 130 140 150
YFNRHFKEPI ACVSDSMTED AAVFGGNNNM NLGLQNASAL YKPEIIAVST
160 170 180 190 200
TCMAEVIGDD LQAFIANAKK DGFVDSSIAV PHAHTPSFIG SHVTGWDNMF
210 220 230 240 250
EGFAKTFTAD YQGQPGKLPK LNLVTGFETY LGNFRVLKRM MEQMAVPCSL
260 270 280 290 300
LSDPSEVLDT PADGHYRMYS GGTTQQEMKE APDAIDTLLL QPWQLLKSKK
310 320 330 340 350
VVQEMWNQPA TEVAIPLGLA ATDELLMTVS QLSGKPIADA LTLERGRLVD
360 370 380 390 400
MMLDSHTWLH GKKFGLYGDP DFVMGLTRFL LELGCEPTVI LSHNANKRWQ
410 420 430 440 450
KAMNKMLDAS PYGRDSEVFI NCDLWHFRSL MFTRQPDFMI GNSYGKFIQR
460 470 480 490 500
DTLAKGKAFE VPLIRLGFPL FDRHHLHRQT TWGYEGAMNI VTTLVNAVLE
510 520
KLDSDTSQLG KTDYSFDLVR
Length:520
Mass (Da):58,408
Last modified:July 1, 1989 - v1
Checksum:iF51C455ECD0DF5C9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24F → V in CAA31180 (PubMed:3211766).Curated1
Sequence conflicti33A → R in CAA31180 (PubMed:3211766).Curated1
Sequence conflicti57 – 58RR → H in CAA31180 (PubMed:3211766).Curated2
Sequence conflicti57R → Q (PubMed:3043382).Curated1
Sequence conflicti57R → Q (PubMed:3322261).Curated1
Sequence conflicti70 – 71QP → HA in CAA31180 (PubMed:3211766).Curated2
Sequence conflicti99R → G in CAA31180 (PubMed:3211766).Curated1
Sequence conflicti163A → R in CAA31180 (PubMed:3211766).Curated1
Sequence conflicti180V → W in CAA31180 (PubMed:3211766).Curated1
Sequence conflicti221L → LKL (PubMed:3211766).Curated1
Sequence conflicti225T → P in CAA31180 (PubMed:3211766).Curated1
Sequence conflicti232G → GTG (PubMed:3211766).Curated1
Sequence conflicti265H → Q in CAA31180 (PubMed:3211766).Curated1
Sequence conflicti287 – 290TLLL → AAP in CAA29588 (PubMed:3322261).Curated4
Sequence conflicti351 – 352MM → IV in CAA29588 (PubMed:3322261).Curated2
Sequence conflicti357Missing in CAA29588 (PubMed:3322261).Curated1
Sequence conflicti395 – 400ANKRWQ → GQQTLD in CAA29588 (PubMed:3322261).Curated6
Sequence conflicti411P → R in CAA29588 (PubMed:3322261).Curated1
Sequence conflicti412 – 417YGRDSE → NRARYS in CAA31180 (PubMed:3211766).Curated6
Sequence conflicti422C → R in CAA29588 (PubMed:3322261).Curated1
Sequence conflicti435 – 438QPDF → SAGL in CAA29588 (PubMed:3322261).Curated4
Sequence conflicti451D → V in CAA31180 (PubMed:3211766).Curated1
Sequence conflicti482W → S in CAA29588 (PubMed:3322261).Curated1
Sequence conflicti497A → V in CAA29588 (PubMed:3322261).Curated1
Sequence conflicti508 – 509QL → PA in CAA29588 (PubMed:3322261).Curated2
Sequence conflicti510G → A in CAA31180 (PubMed:3211766).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13303 Genomic DNA. Translation: CAA31668.1.
X07749 Genomic DNA. Translation: CAA30573.1.
X06243 Genomic DNA. Translation: CAA29588.1.
X12649 Genomic DNA. Translation: CAA31180.1.
PIRiS02505.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiNIFK_KLEPN
AccessioniPrimary (citable) accession number: P09772
Secondary accession number(s): P09771
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 2, 2016
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

The authors of PubMed:3211766 originally stated that their sequence was from Frankia sp.Curated

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families