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P09768 (KITH_CRIGR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Thymidine kinase, cytosolic
EC=2.7.1.21
Gene names
Name:TK1
OrganismCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifier10029 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

Protein attributes

Sequence length234 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + thymidine = ADP + thymidine 5'-phosphate.

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm.

Post-translational modification

Phosphorylated on Ser-13 in mitosis By similarity.

Miscellaneous

Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Sequence similarities

Belongs to the thymidine kinase family.

Ontologies

Keywords
   Biological processDNA synthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionKinase
Transferase
   PTMPhosphoprotein
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

thymidine kinase activity

Inferred from electronic annotation. Source: EC

zinc ion binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 234233Thymidine kinase, cytosolic
PRO_0000174947

Regions

Nucleotide binding26 – 338ATP Probable
Nucleotide binding58 – 603ATP By similarity
Nucleotide binding97 – 1004ATP By similarity
Region172 – 1765Substrate binding By similarity

Sites

Active site981Proton acceptor Potential
Metal binding1531Zinc By similarity
Metal binding1561Zinc By similarity
Metal binding1851Zinc By similarity
Metal binding1881Zinc By similarity
Binding site1281Substrate; via amide nitrogen By similarity
Binding site1811Substrate By similarity

Amino acid modifications

Modified residue131Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
P09768 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 8E46DC89FEAF2E9C

FASTA23425,622
        10         20         30         40         50         60 
MNYINLPTVL PGSPSKTRGQ IQVILGPMFS GKSTELMRRV RRFQIAQNKC LVIKYAKDTR 

        70         80         90        100        110        120 
YSSSFSTHDR NTMDALPACL LRDVAQEALG AAVIGIDEGQ FFPDIVEFCE VMANAGKTVI 

       130        140        150        160        170        180 
VAALDGTFQR KAFGSILNLV PLAESVVKLT AVCMECFREA AYTKRLGLEK EVEVIGGADK 

       190        200        210        220        230 
YHSVCRVCYF KKSSVQPAGP DNKENCPVLG QPGEASAVRK LFAPQQVLQH NSTN

« Hide

References

[1]"Structure and expression of the Chinese hamster thymidine kinase gene."
Lewis J.A.
Mol. Cell. Biol. 6:1998-2010(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L00369 expand/collapse EMBL AC list , L00364, L00365, L00366, L00367, L00368 Genomic DNA. Translation: AAA37022.1.
PIRA25243.
RefSeqNP_001231423.1. NM_001244494.1.

3D structure databases

ProteinModelPortalP09768.
SMRP09768. Positions 18-191.
ModBaseSearch...

Proteomic databases

PRIDEP09768.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100689285.

Organism-specific databases

CTD7083.

Phylogenomic databases

HOVERGENHBG006215.

Family and domain databases

InterProIPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERPTHR11441. PTHR11441. 1 hit.
PfamPF00265. TK. 1 hit.
[Graphical view]
PIRSFPIRSF035805. TK_cell. 1 hit.
PROSITEPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKITH_CRIGR
AccessionPrimary (citable) accession number: P09768
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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