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P09768

- KITH_CRIGR

UniProt

P09768 - KITH_CRIGR

Protein

Thymidine kinase, cytosolic

Gene

TK1

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + thymidine = ADP + thymidine 5'-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei98 – 981Proton acceptorSequence Analysis
    Binding sitei128 – 1281Substrate; via amide nitrogenBy similarity
    Metal bindingi153 – 1531ZincBy similarity
    Metal bindingi156 – 1561ZincBy similarity
    Binding sitei181 – 1811SubstrateBy similarity
    Metal bindingi185 – 1851ZincBy similarity
    Metal bindingi188 – 1881ZincBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi26 – 338ATPCurated
    Nucleotide bindingi58 – 603ATPBy similarity
    Nucleotide bindingi97 – 1004ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. thymidine kinase activity Source: UniProtKB-EC
    3. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinase, cytosolic (EC:2.7.1.21)
    Gene namesi
    Name:TK1
    OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
    Taxonomic identifieri10029 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 234233Thymidine kinase, cytosolicPRO_0000174947Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei13 – 131PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated on Ser-13 in mitosis.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP09768.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP09768.
    SMRiP09768. Positions 18-191.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni172 – 1765Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the thymidine kinase family.Curated

    Phylogenomic databases

    HOVERGENiHBG006215.
    KOiK00857.

    Family and domain databases

    InterProiIPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view]
    PANTHERiPTHR11441. PTHR11441. 1 hit.
    PfamiPF00265. TK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF035805. TK_cell. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09768-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNYINLPTVL PGSPSKTRGQ IQVILGPMFS GKSTELMRRV RRFQIAQNKC    50
    LVIKYAKDTR YSSSFSTHDR NTMDALPACL LRDVAQEALG AAVIGIDEGQ 100
    FFPDIVEFCE VMANAGKTVI VAALDGTFQR KAFGSILNLV PLAESVVKLT 150
    AVCMECFREA AYTKRLGLEK EVEVIGGADK YHSVCRVCYF KKSSVQPAGP 200
    DNKENCPVLG QPGEASAVRK LFAPQQVLQH NSTN 234
    Length:234
    Mass (Da):25,622
    Last modified:July 1, 1989 - v1
    Checksum:i8E46DC89FEAF2E9C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L00369
    , L00364, L00365, L00366, L00367, L00368 Genomic DNA. Translation: AAA37022.1.
    PIRiA25243.
    RefSeqiNP_001231423.1. NM_001244494.1.

    Genome annotation databases

    GeneIDi100689285.
    KEGGicge:100689285.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L00369
    , L00364 , L00365 , L00366 , L00367 , L00368 Genomic DNA. Translation: AAA37022.1 .
    PIRi A25243.
    RefSeqi NP_001231423.1. NM_001244494.1.

    3D structure databases

    ProteinModelPortali P09768.
    SMRi P09768. Positions 18-191.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P09768.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100689285.
    KEGGi cge:100689285.

    Organism-specific databases

    CTDi 7083.

    Phylogenomic databases

    HOVERGENi HBG006215.
    KOi K00857.

    Family and domain databases

    InterProi IPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view ]
    PANTHERi PTHR11441. PTHR11441. 1 hit.
    Pfami PF00265. TK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF035805. TK_cell. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and expression of the Chinese hamster thymidine kinase gene."
      Lewis J.A.
      Mol. Cell. Biol. 6:1998-2010(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiKITH_CRIGR
    AccessioniPrimary (citable) accession number: P09768
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3