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P09768

- KITH_CRIGR

UniProt

P09768 - KITH_CRIGR

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Protein

Thymidine kinase, cytosolic

Gene
TK1
Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei98 – 981Proton acceptor Reviewed prediction
Binding sitei128 – 1281Substrate; via amide nitrogen By similarity
Metal bindingi153 – 1531Zinc By similarity
Metal bindingi156 – 1561Zinc By similarity
Binding sitei181 – 1811Substrate By similarity
Metal bindingi185 – 1851Zinc By similarity
Metal bindingi188 – 1881Zinc By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 338ATP Inferred
Nucleotide bindingi58 – 603ATP By similarity
Nucleotide bindingi97 – 1004ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. thymidine kinase activity Source: UniProtKB-EC
  3. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase, cytosolic (EC:2.7.1.21)
Gene namesi
Name:TK1
OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifieri10029 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 234233Thymidine kinase, cytosolicPRO_0000174947Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei13 – 131Phosphoserine By similarity

Post-translational modificationi

Phosphorylated on Ser-13 in mitosis By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP09768.

Interactioni

Subunit structurei

Homotetramer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP09768.
SMRiP09768. Positions 18-191.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni172 – 1765Substrate binding By similarity

Sequence similaritiesi

Belongs to the thymidine kinase family.

Phylogenomic databases

HOVERGENiHBG006215.
KOiK00857.

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09768-1 [UniParc]FASTAAdd to Basket

« Hide

MNYINLPTVL PGSPSKTRGQ IQVILGPMFS GKSTELMRRV RRFQIAQNKC    50
LVIKYAKDTR YSSSFSTHDR NTMDALPACL LRDVAQEALG AAVIGIDEGQ 100
FFPDIVEFCE VMANAGKTVI VAALDGTFQR KAFGSILNLV PLAESVVKLT 150
AVCMECFREA AYTKRLGLEK EVEVIGGADK YHSVCRVCYF KKSSVQPAGP 200
DNKENCPVLG QPGEASAVRK LFAPQQVLQH NSTN 234
Length:234
Mass (Da):25,622
Last modified:July 1, 1989 - v1
Checksum:i8E46DC89FEAF2E9C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L00369
, L00364, L00365, L00366, L00367, L00368 Genomic DNA. Translation: AAA37022.1.
PIRiA25243.
RefSeqiNP_001231423.1. NM_001244494.1.

Genome annotation databases

GeneIDi100689285.
KEGGicge:100689285.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L00369
, L00364 , L00365 , L00366 , L00367 , L00368 Genomic DNA. Translation: AAA37022.1 .
PIRi A25243.
RefSeqi NP_001231423.1. NM_001244494.1.

3D structure databases

ProteinModelPortali P09768.
SMRi P09768. Positions 18-191.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P09768.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100689285.
KEGGi cge:100689285.

Organism-specific databases

CTDi 7083.

Phylogenomic databases

HOVERGENi HBG006215.
KOi K00857.

Family and domain databases

InterProi IPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view ]
PANTHERi PTHR11441. PTHR11441. 1 hit.
Pfami PF00265. TK. 1 hit.
[Graphical view ]
PIRSFi PIRSF035805. TK_cell. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Structure and expression of the Chinese hamster thymidine kinase gene."
    Lewis J.A.
    Mol. Cell. Biol. 6:1998-2010(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiKITH_CRIGR
AccessioniPrimary (citable) accession number: P09768
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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