Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thymidine kinase, cytosolic

Gene

TK1

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Miscellaneous

Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei98Proton acceptorSequence analysis1
Binding sitei128Substrate; via amide nitrogenBy similarity1
Metal bindingi153ZincBy similarity1
Metal bindingi156ZincBy similarity1
Binding sitei181SubstrateBy similarity1
Metal bindingi185ZincBy similarity1
Metal bindingi188ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 33ATPCurated8
Nucleotide bindingi58 – 60ATPBy similarity3
Nucleotide bindingi97 – 100ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processDNA synthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase, cytosolic (EC:2.7.1.21)
Gene namesi
Name:TK1
OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifieri10029 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaCricetidaeCricetinaeCricetulus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3227916.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001749471 – 234Thymidine kinase, cytosolicAdd BLAST234

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on Ser-13 in mitosis.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP09768.

Interactioni

Subunit structurei

Homotetramer.By similarity

Chemistry databases

BindingDBiP09768.

Structurei

3D structure databases

ProteinModelPortaliP09768.
SMRiP09768.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni172 – 176Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the thymidine kinase family.Curated

Phylogenomic databases

HOVERGENiHBG006215.
KOiK00857.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiView protein in Pfam
PF00265. TK. 1 hit.
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS00603. TK_CELLULAR_TYPE. 1 hit.

Sequencei

Sequence statusi: Complete.

P09768-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYINLPTVL PGSPSKTRGQ IQVILGPMFS GKSTELMRRV RRFQIAQNKC
60 70 80 90 100
LVIKYAKDTR YSSSFSTHDR NTMDALPACL LRDVAQEALG AAVIGIDEGQ
110 120 130 140 150
FFPDIVEFCE VMANAGKTVI VAALDGTFQR KAFGSILNLV PLAESVVKLT
160 170 180 190 200
AVCMECFREA AYTKRLGLEK EVEVIGGADK YHSVCRVCYF KKSSVQPAGP
210 220 230
DNKENCPVLG QPGEASAVRK LFAPQQVLQH NSTN
Length:234
Mass (Da):25,622
Last modified:July 1, 1989 - v1
Checksum:i8E46DC89FEAF2E9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00369
, L00364, L00365, L00366, L00367, L00368 Genomic DNA. Translation: AAA37022.1.
PIRiA25243.
RefSeqiNP_001231423.1. NM_001244494.2.

Genome annotation databases

GeneIDi100689285.
KEGGicge:100689285.

Similar proteinsi

Entry informationi

Entry nameiKITH_CRIGR
AccessioniPrimary (citable) accession number: P09768
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: August 30, 2017
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families