Reviewed, UniProtKB/Swiss-Prot P09759 (EPHB1_RAT)

Names and origin

Protein names

Recommended name:
    Ephrin type-B receptor 1
    EC=2.7.10.1
Alternative name(s):
    Tyrosine-protein kinase receptor EPH-2
    ELK

Gene names

Name:	Ephb1
Synonyms:	Elk, Epth2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	984 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Receptor for members of the ephrin-B family. Binds to ephrin-B1, -B2 and -B3. May be involved in cell-cell interactions in the nervous system.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure	The ligand-activated form interacts with GRB2, GRB10 and NCK through their respective SH2 domains. The GRB10 SH2 domain binds EPHB1 through Tyr-928, while GRB2 binds residues within the catalytic domain. The NCK SH2 domain binds EPHB1 through Tyr-594. Interacts with EPHB6 and PRKCABP By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Restricted to brain and testes.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. Contains 2 fibronectin type-III domains. Contains 1 protein kinase domain. Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat Signal Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Transferase Tyrosine-protein kinase
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	cell-cell signaling Ref.1 Inferred from physical interaction. Source: RGD protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro transmembrane receptor protein tyrosine kinase signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ephrin receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 984	967	Ephrin type-B receptor 1		PRO_0000016825
Regions
	Topological domain	18 – 540	523	Extracellular Potential
	Transmembrane	541 – 563	23	Potential
	Topological domain	564 – 984	421	Cytoplasmic Potential
	Domain	323 – 424	102	Fibronectin type-III 1
	Domain	430 – 525	96	Fibronectin type-III 2
	Domain	619 – 882	264	Protein kinase
	Domain	911 – 975	65	SAM
	Nucleotide binding	625 – 633	9	ATP By similarity
	Motif	982 – 984	3	PDZ-binding Potential
	Compositional bias	183 – 319	137	Cys-rich
Sites
	Active site	744	1	Proton acceptor By similarity
	Binding site	651	1	ATP By similarity
Amino acid modifications
	Modified residue	928	1	Phosphotyrosine; by autocatalysis By similarity
	Modified residue	968	1	Phosphoserine By similarity
	Glycosylation	334	1	N-linked (GlcNAc...) Potential
	Glycosylation	426	1	N-linked (GlcNAc...) Potential
	Glycosylation	480	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P09759-1 [UniParc]. Last modified December 1, 1992. Version 2. Checksum: 521EAC240D8FB91A	FASTA	984	109,883
	10 20 30 40 50 60 MALDCLLLFL LASAVAAMEE TLMDTRTATA ELGWTANPAS GWEEVSGYDE NLNTIRTYQV 70 80 90 100 110 120 CNVFEPNQNN WLLTTFINRR GAHRIYTEMR FTVRDCSSLP NVPGSCKETF NLYYYETDSV 130 140 150 160 170 180 IATKKSAFWS EAPYLKVDTI AADESFSQVD FGGRLMKVNT EVRSFGPLTR NGFYLAFQDY 190 200 210 220 230 240 GACMSLLSVR VFFKKCPSIV QNFAVFPETM TGAESTSLVI ARGTCIPNAE EVDVPIKLYC 250 260 270 280 290 300 NGDGEWMVPI GRCTCKAGYE PENSVACKAC PAGTFKASQE AEGCSHCPSN SRSPSEASPI 310 320 330 340 350 360 CTCRTGYYRA DFDPPEVACT SVPSGPRNVI SIVNETSIIL EWHPPRETGG RDDVTYNIIC 370 380 390 400 410 420 KKCRADRRSC SRCDDNVEFV PRQLGLTECR VSISSLWAHT PYTFDIQAIN GVSSKSPFPP 430 440 450 460 470 480 QHVSVNITTN QAAPSTVPIM HQVSATMRSI TLSWPQPEQP NGIILDYEIR YYEKEHNEFN 490 500 510 520 530 540 SSMARSQTNT ARIDGLRPGM VYVVQVRART VAGYGKFSGK MCFQTLTDDD YKSELREQLP 550 560 570 580 590 600 LIAGSAAAGV VFVVSLVAIS IVCSRKRAYS KEAVYSDKLQ HYSTGRGSPG MKIYIDPFTY 610 620 630 640 650 660 EDPNEAVREF AKEIDVSFVK IEEVIGAGEF GEVYKGRLKL PGKREIYVAI KTLKAGYSEK 670 680 690 700 710 720 QRRDFLSEAS IMGQFDHPNI IRLEGVVTKS RPVMIITEFM ENGALDSFLR QNDGQFTVIQ 730 740 750 760 770 780 LVGMLRGIAA GMKYLSEMNY VHRDLAARNI LVNSNLVCKV SDFGLSRYLQ DDTSDPTYTS 790 800 810 820 830 840 SLGGKIPVRW TAPEAIAYRK FTSASDVWSY GIVMWEVMSF GERPYWDMSN QDVINAIEQD 850 860 870 880 890 900 YRLPPPMDCP AALHQLMLDC WQKDRNSRPR FAEIVNTLDK MIRNPASLKT VATITAVPSQ 910 920 930 940 950 960 PLLDRSIPDF TAFTTVDDWL SAIKMVQYRD SFLTAGFTSL QLVTQMTSED LLRIGVTLAG 970 980 HQKKILSSIH SMRVQMNQSP SVMA

References

[1]	"Characterization of elk, a brain-specific receptor tyrosine kinase." Lhotak V., Greer P., Letwin K., Pawson T. Mol. Cell. Biol. 11:2496-2502(1991) [PubMed: 2017163] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Brain.
[2]	"Novel protein-tyrosine kinase cDNAs related to fps/fes and eph cloned using anti-phosphotyrosine antibody." Letwin K., Yee S.P., Pawson T. Oncogene 3:621-627(1988) [PubMed: 2485255] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 605-984. Strain: Wistar. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M59814 mRNA. No translation available. X13411 mRNA. Translation: CAA31777.1.
IPI	IPI00189428.
PIR	A39753.
RefSeq	NP_001097998.1.
UniGene	Rn.46606
3D structure databases
HSSP	HSSP built from PDB template 1JPA based on UniProtKB P54763.
SMR	P09759. Positions 18-197, 436-528, 592-889.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:138N.
STRING	P09759.
PTM databases
PhosphoSite	P09759.
Genome annotation databases
Ensembl	ENSRNOT00000010634; ENSRNOP00000010634; ENSRNOG00000007865; Rattus norvegicus. [Genome view]
GeneID	24338.
KEGG	rno:24338.
UCSC	M59814. rat.
Organism-specific databases
CTD	24338.
RGD	2556. Ephb1.
Phylogenomic databases
HOVERGEN	P09759.
Enzyme and pathway databases
BRENDA	2.7.10.1. 248.
Gene expression databases
ArrayExpress	P09759.
Genevestigator	P09759.
GermOnline	ENSRNOG00000007865. Rattus norvegicus.
Family and domain databases
InterPro	IPR013032. EGF-like_reg_CS. IPR001090. Ephrin_rcpt_lig-bd. IPR008957. Fibronectin_typ-III-like_fold. IPR003961. FN_III. IPR003962. FnIII_subd. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR001660. SAM. IPR013761. SAM_type. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. IPR016257. TyrPK_ephrin_receptor. IPR001426. YKase_receptorV_CS. [Graphical view]
Gene3D	G3DSA:2.60.40.30. FN_III-like. 2 hits. G3DSA:1.10.150.50. SAM_type. 1 hit.
Pfam	PF01404. Ephrin_lbd. 1 hit. PF00041. fn3. 2 hits. PF07714. Pkinase_Tyr. 1 hit. PF00536. SAM_1. 1 hit. [Graphical view]
PIRSF	PIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTS	PR00014. FNTYPEIII. PR00109. TYRKINASE.
ProDom	PD001495. Ephrin_receptor. 1 hit. PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00615. EPH_lbd. 1 hit. SM00060. FN3. 2 hits. SM00454. SAM. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS01186. EGF_2. 1 hit. Uncertain. PS50853. FN3. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit. PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit. PS50105. SAM_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	603035.

Entry information

Entry name

EPHB1_RAT

Accession

Primary (citable) accession number: P09759

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	December 1, 1992
Last modified:	October 13, 2009
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)