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P09734 (TBA3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tubulin alpha-3 chain
Gene names
Name:TUB3
Ordered Locus Names:YML124C
ORF Names:YM7056.02C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Subunit structure

Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Interacts with NUM1. Ref.6

Subcellular location

Cytoplasmcytoskeleton.

Miscellaneous

Present with 12300 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the tubulin family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

NUM1Q004022EBI-18981,EBI-12386

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 445445Tubulin alpha-3 chain
PRO_0000048240

Regions

Nucleotide binding143 – 1497GTP Potential

Sequences

Sequence LengthMass (Da)Tools
P09734 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 189889B795E72448

FASTA44549,694
        10         20         30         40         50         60 
MREVISINVG QAGCQIGNAC WELYSLEHGI KEDGHLEDGL SKPKGGEEGF STFFHETGYG 

        70         80         90        100        110        120 
KFVPRAIYVD LEPNVIDEVR TGRFKELFHP EQLINGKEDA ANNYARGHYT VGREIVDEVE 

       130        140        150        160        170        180 
ERIRKMADQC DGLQGFLFTH SLGGGTGSGL GSLLLENLSY EYGKKSKLEF AVYPAPQLST 

       190        200        210        220        230        240 
SVVEPYNTVL TTHTTLEHAD CTFMVDNEAI YDICKRNLGI SRPSFSNLNG LIAQVISSVT 

       250        260        270        280        290        300 
ASLRFDGSLN VDLNEFQTNL VPYPRIHFPL VSYAPILSKK RATHESNSVS EITNACFEPG 

       310        320        330        340        350        360 
NQMVKCDPTK GKYMANCLLY RGDVVTRDVQ RAVEQVKNKK TVQMVDWCPT GFKIGICYEP 

       370        380        390        400        410        420 
PSVIPSSELA NVDRAVCMLS NTTAIADAWK RIDQKFDLMY AKRAFVHWYV GEGMEEGEFT 

       430        440 
EAREDLAALE RDYIEVGADS YAEEF 

« Hide

References

« Hide 'large scale' references
[1]"Two functional alpha-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins."
Schatz P.J., Pillus L., Grisafi P., Solomon F., Botstein D.
Mol. Cell. Biol. 6:3711-3721(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter."
Bun-Ya M., Nishimura M., Harashima S., Oshima Y.
Mol. Cell. Biol. 11:3229-3238(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-170.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Cortical Num1p interacts with the dynein intermediate chain Pac11p and cytoplasmic microtubules in budding yeast."
Farkasovsky M., Kuentzel H.
J. Cell Biol. 152:251-262(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NUM1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M28428 mRNA. Translation: AAA35181.1.
Z49218 Genomic DNA. Translation: CAA89156.1.
D90346 Genomic DNA. No translation available.
BK006946 Genomic DNA. Translation: DAA09775.1.
PIRB25076.
RefSeqNP_013582.1. NM_001182487.1.

3D structure databases

ProteinModelPortalP09734.
SMRP09734. Positions 1-439.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35081. 159 interactions.
DIPDIP-2206N.
IntActP09734. 63 interactions.
MINTMINT-617389.
STRING4932.YML124C.

Proteomic databases

PaxDbP09734.
PeptideAtlasP09734.
PRIDEP09734.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML124C; YML124C; YML124C.
GeneID854915.
KEGGsce:YML124C.

Organism-specific databases

CYGDYML124c.
SGDS000004593. TUB3.

Phylogenomic databases

eggNOGCOG5023.
GeneTreeENSGT00750000117755.
HOGENOMHOG000165711.
OMAKRATHES.
OrthoDBEOG7PVWZW.

Enzyme and pathway databases

BioCycYEAST:G3O-32703-MONOMER.

Gene expression databases

GenevestigatorP09734.

Family and domain databases

Gene3D1.10.287.600. 1 hit.
3.30.1330.20. 1 hit.
3.40.50.1440. 1 hit.
InterProIPR002452. Alpha_tubulin.
IPR008280. Tub_FtsZ_C.
IPR000217. Tubulin.
IPR018316. Tubulin/FtsZ_2-layer-sand-dom.
IPR023123. Tubulin_C.
IPR017975. Tubulin_CS.
IPR003008. Tubulin_FtsZ_GTPase.
[Graphical view]
PANTHERPTHR11588. PTHR11588. 1 hit.
PfamPF00091. Tubulin. 1 hit.
PF03953. Tubulin_C. 1 hit.
[Graphical view]
PRINTSPR01162. ALPHATUBULIN.
PR01161. TUBULIN.
SMARTSM00864. Tubulin. 1 hit.
SM00865. Tubulin_C. 1 hit.
[Graphical view]
SUPFAMSSF52490. SSF52490. 1 hit.
SSF55307. SSF55307. 1 hit.
PROSITEPS00227. TUBULIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977920.

Entry information

Entry nameTBA3_YEAST
AccessionPrimary (citable) accession number: P09734
Secondary accession number(s): D6W0G1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families