Tubulin alpha-3 chain - TUB3 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P09734 (TBA3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 124. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Tubulin alpha-3 chain

Gene names

Name:	TUB3
Ordered Locus Names:	YML124C
ORF Names:	YM7056.02C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Subunit structure	Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Interacts with NUM1. Ref.6
Subcellular location	Cytoplasm › cytoskeleton.
Miscellaneous	Present with 12300 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the tubulin family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Microtubule
Ligand	GTP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	homologous chromosome segregation Inferred by curator PubMed 3540600. Source: SGD mitotic sister chromatid segregation Inferred by curator PubMed 3540600. Source: SGD nuclear migration along microtubule Inferred by curator PubMed 3540600. Source: SGD protein polymerization Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasmic microtubule Inferred by curator PubMed 3540600. Source: SGD kinetochore microtubule Inferred by curator PubMed 3540600. Source: SGD nuclear microtubule Inferred by curator PubMed 3540600. Source: SGD spindle pole body Inferred from direct assay PubMed 9585415. Source: SGD tubulin complex Inferred from genetic interaction PubMed 3540600. Source: SGD
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction Ref.6. Source: IntAct structural constituent of cytoskeleton Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NUM1	Q00402	2	EBI-18981,EBI-12386

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 445

445

Tubulin alpha-3 chain

PRO_0000048240

Regions

Nucleotide binding

143 – 149

GTP Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P09734 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 189889B795E72448
Show »

FASTA

445

49,694

        10         20         30         40         50         60 
MREVISINVG QAGCQIGNAC WELYSLEHGI KEDGHLEDGL SKPKGGEEGF STFFHETGYG 

        70         80         90        100        110        120 
KFVPRAIYVD LEPNVIDEVR TGRFKELFHP EQLINGKEDA ANNYARGHYT VGREIVDEVE 

       130        140        150        160        170        180 
ERIRKMADQC DGLQGFLFTH SLGGGTGSGL GSLLLENLSY EYGKKSKLEF AVYPAPQLST 

       190        200        210        220        230        240 
SVVEPYNTVL TTHTTLEHAD CTFMVDNEAI YDICKRNLGI SRPSFSNLNG LIAQVISSVT 

       250        260        270        280        290        300 
ASLRFDGSLN VDLNEFQTNL VPYPRIHFPL VSYAPILSKK RATHESNSVS EITNACFEPG 

       310        320        330        340        350        360 
NQMVKCDPTK GKYMANCLLY RGDVVTRDVQ RAVEQVKNKK TVQMVDWCPT GFKIGICYEP 

       370        380        390        400        410        420 
PSVIPSSELA NVDRAVCMLS NTTAIADAWK RIDQKFDLMY AKRAFVHWYV GEGMEEGEFT 

       430        440 
EAREDLAALE RDYIEVGADS YAEEF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two functional alpha-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins." Schatz P.J., Pillus L., Grisafi P., Solomon F., Botstein D. Mol. Cell. Biol. 6:3711-3721(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The reference genome sequence of Saccharomyces cerevisiae: Then and now." Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M. G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter." Bun-Ya M., Nishimura M., Harashima S., Oshima Y. Mol. Cell. Biol. 11:3229-3238(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-170.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Cortical Num1p interacts with the dynein intermediate chain Pac11p and cytoplasmic microtubules in budding yeast." Farkasovsky M., Kuentzel H. J. Cell Biol. 152:251-262(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NUM1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M28428 mRNA. Translation: AAA35181.1. Z49218 Genomic DNA. Translation: CAA89156.1. D90346 Genomic DNA. No translation available. BK006946 Genomic DNA. Translation: DAA09775.1.
PIR	B25076.
RefSeq	NP_013582.1. NM_001182487.1.
3D structure databases
ProteinModelPortal	P09734.
SMR	P09734. Positions 1-439.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	35081. 159 interactions.
DIP	DIP-2206N.
IntAct	P09734. 63 interactions.
MINT	MINT-617389.
STRING	4932.YML124C.
Proteomic databases
MaxQB	P09734.
PaxDb	P09734.
PeptideAtlas	P09734.
PRIDE	P09734.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML124C; YML124C; YML124C.
GeneID	854915.
KEGG	sce:YML124C.
Organism-specific databases
CYGD	YML124c.
SGD	S000004593. TUB3.
Phylogenomic databases
eggNOG	COG5023.
GeneTree	ENSGT00750000117755.
HOGENOM	HOG000165711.
OMA	TRATHEA.
OrthoDB	EOG7PVWZW.
Enzyme and pathway databases
BioCyc	YEAST:G3O-32703-MONOMER.
Gene expression databases
Genevestigator	P09734.
Family and domain databases
Gene3D	1.10.287.600. 1 hit. 3.30.1330.20. 1 hit. 3.40.50.1440. 1 hit.
InterPro	IPR002452. Alpha_tubulin. IPR008280. Tub_FtsZ_C. IPR000217. Tubulin. IPR018316. Tubulin/FtsZ_2-layer-sand-dom. IPR023123. Tubulin_C. IPR017975. Tubulin_CS. IPR003008. Tubulin_FtsZ_GTPase. [Graphical view]
PANTHER	PTHR11588. PTHR11588. 1 hit.
Pfam	PF00091. Tubulin. 1 hit. PF03953. Tubulin_C. 1 hit. [Graphical view]
PRINTS	PR01162. ALPHATUBULIN. PR01161. TUBULIN.
SMART	SM00864. Tubulin. 1 hit. SM00865. Tubulin_C. 1 hit. [Graphical view]
SUPFAM	SSF52490. SSF52490. 1 hit. SSF55307. SSF55307. 1 hit.
PROSITE	PS00227. TUBULIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977920.

Entry information

Entry name

TBA3_YEAST

Accession

Primary (citable) accession number: P09734
Secondary accession number(s): D6W0G1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	June 11, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents