TUB3

Functionⁱ

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Miscellaneous

Present with 12300 molecules/cell in log phase SD medium.1 Publication

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	143 – 149	GTPSequence analysis		7

GO - Molecular functionⁱ

GTPase activity Source: InterPro
GTP binding Source: UniProtKB-KW
structural constituent of cytoskeleton Source: InterPro

Complete GO annotation...

GO - Biological processⁱ

Complete GO annotation...

Keywordsⁱ

Ligand	GTP-binding, Nucleotide-binding

Ligand

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-32703-MONOMER.
Reactomeⁱ	R-SCE-114608. Platelet degranulation. R-SCE-5610787. Hedgehog 'off' state. R-SCE-5617833. Cilium Assembly. R-SCE-5626467. RHO GTPases activate IQGAPs. R-SCE-6811434. COPI-dependent Golgi-to-ER retrograde traffic. R-SCE-983189. Kinesins.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Tubulin alpha-3 chain
Gene namesⁱ	Name:TUB3 Ordered Locus Names:YML124C ORF Names:YM7056.02C
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome XIII

Organism-specific databases

EuPathDBⁱ	FungiDB:YML124C.
Saccharomyces Genome Database More...SGDⁱ	S000004593. TUB3.

Subcellular locationⁱ

Cytoplasm › cytoskeleton

GO - Cellular componentⁱ

cytoplasmic microtubule
Source: SGD
Inferred by curatorⁱ
- 3540600
kinetochore microtubule
Source: SGD
Inferred by curatorⁱ
- 3540600
nuclear microtubule
Source: SGD
Inferred by curatorⁱ
- 3540600
spindle pole body
Source: SGD
Inferred from direct assayⁱ
- 9585415
tubulin complex
Source: SGD
Inferred from genetic interactionⁱ
- 3540600

Complete GO annotation...

Keywords - Cellular componentⁱ

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000048240	1 – 445	Tubulin alpha-3 chainAdd BLAST		445

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P09734.
PRIDEⁱ	P09734.

Interactionⁱ

Subunit structureⁱ

Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Interacts with NUM1.1 Publication

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
NUM1	Q00402	2	EBI-18981,EBI-12386

With

Entry

#Exp.

IntAct

Notes

NUM1

Q00402

2

EBI-18981,EBI-12386

Protein-protein interaction databases

BioGridⁱ	35081. 213 interactors.
Database of interacting proteins More...DIPⁱ	DIP-2206N.
IntActⁱ	P09734. 64 interactors.
Molecular INTeraction database More...MINTⁱ	MINT-617389.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P09734.
SMRⁱ	P09734.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the tubulin family.Curated

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00880000138422.
HOGENOMⁱ	HOG000165711.
InParanoidⁱ	P09734.
OMAⁱ	ICNEPSA.
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C2JRV.

Family and domain databases

Gene3Dⁱ	3.30.1330.20. 1 hit. 3.40.50.1440. 1 hit.
InterProⁱ	View protein in InterPro IPR002452. Alpha_tubulin. IPR008280. Tub_FtsZ_C. IPR000217. Tubulin. IPR018316. Tubulin/FtsZ_2-layer-sand-dom. IPR017975. Tubulin_CS. IPR003008. Tubulin_FtsZ_GTPase.
PANTHERⁱ	PTHR11588. PTHR11588. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00091. Tubulin. 1 hit. PF03953. Tubulin_C. 1 hit.
PRINTSⁱ	PR01162. ALPHATUBULIN. PR01161. TUBULIN.
SMARTⁱ	View protein in SMART SM00864. Tubulin. 1 hit. SM00865. Tubulin_C. 1 hit.
SUPFAMⁱ	SSF52490. SSF52490. 1 hit. SSF55307. SSF55307. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00227. TUBULIN. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

P09734-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MREVISINVG QAGCQIGNAC WELYSLEHGI KEDGHLEDGL SKPKGGEEGF 
        60         70         80         90        100
STFFHETGYG KFVPRAIYVD LEPNVIDEVR TGRFKELFHP EQLINGKEDA 
       110        120        130        140        150
ANNYARGHYT VGREIVDEVE ERIRKMADQC DGLQGFLFTH SLGGGTGSGL 
       160        170        180        190        200
GSLLLENLSY EYGKKSKLEF AVYPAPQLST SVVEPYNTVL TTHTTLEHAD 
       210        220        230        240        250
CTFMVDNEAI YDICKRNLGI SRPSFSNLNG LIAQVISSVT ASLRFDGSLN 
       260        270        280        290        300
VDLNEFQTNL VPYPRIHFPL VSYAPILSKK RATHESNSVS EITNACFEPG 
       310        320        330        340        350
NQMVKCDPTK GKYMANCLLY RGDVVTRDVQ RAVEQVKNKK TVQMVDWCPT 
       360        370        380        390        400
GFKIGICYEP PSVIPSSELA NVDRAVCMLS NTTAIADAWK RIDQKFDLMY 
       410        420        430        440 
AKRAFVHWYV GEGMEEGEFT EAREDLAALE RDYIEVGADS YAEEF

Length:445

Mass (Da):49,694

Last modified:July 1, 1989 - v1

Checksum:ⁱ189889B795E72448

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M28428 mRNA. Translation: AAA35181.1. Z49218 Genomic DNA. Translation: CAA89156.1. D90346 Genomic DNA. No translation available. BK006946 Genomic DNA. Translation: DAA09775.1.
PIRⁱ	B25076.
NCBI Reference Sequences More...RefSeqⁱ	NP_013582.1. NM_001182487.1.

Genome annotation databases

EnsemblFungiⁱ	YML124C; YML124C; YML124C.
GeneIDⁱ	854915.
KEGGⁱ	sce:YML124C.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M28428 mRNA. Translation: AAA35181.1. Z49218 Genomic DNA. Translation: CAA89156.1. D90346 Genomic DNA. No translation available. BK006946 Genomic DNA. Translation: DAA09775.1.
PIRⁱ	B25076.
NCBI Reference Sequences More...RefSeqⁱ	NP_013582.1. NM_001182487.1.

3D structure databases

ProteinModelPortalⁱ	P09734.
SMRⁱ	P09734.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	35081. 213 interactors.
Database of interacting proteins More...DIPⁱ	DIP-2206N.
IntActⁱ	P09734. 64 interactors.
Molecular INTeraction database More...MINTⁱ	MINT-617389.

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P09734.
PRIDEⁱ	P09734.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YML124C; YML124C; YML124C.
GeneIDⁱ	854915.
KEGGⁱ	sce:YML124C.

Organism-specific databases

EuPathDBⁱ	FungiDB:YML124C.
Saccharomyces Genome Database More...SGDⁱ	S000004593. TUB3.

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00880000138422.
HOGENOMⁱ	HOG000165711.
InParanoidⁱ	P09734.
OMAⁱ	ICNEPSA.
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C2JRV.

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-32703-MONOMER.
Reactomeⁱ	R-SCE-114608. Platelet degranulation. R-SCE-5610787. Hedgehog 'off' state. R-SCE-5617833. Cilium Assembly. R-SCE-5626467. RHO GTPases activate IQGAPs. R-SCE-6811434. COPI-dependent Golgi-to-ER retrograde traffic. R-SCE-983189. Kinesins.

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P09734.

PROⁱ

PR:P09734.

Family and domain databases

Gene3Dⁱ	3.30.1330.20. 1 hit. 3.40.50.1440. 1 hit.
InterProⁱ	View protein in InterPro IPR002452. Alpha_tubulin. IPR008280. Tub_FtsZ_C. IPR000217. Tubulin. IPR018316. Tubulin/FtsZ_2-layer-sand-dom. IPR017975. Tubulin_CS. IPR003008. Tubulin_FtsZ_GTPase.
PANTHERⁱ	PTHR11588. PTHR11588. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00091. Tubulin. 1 hit. PF03953. Tubulin_C. 1 hit.
PRINTSⁱ	PR01162. ALPHATUBULIN. PR01161. TUBULIN.
SMARTⁱ	View protein in SMART SM00864. Tubulin. 1 hit. SM00865. Tubulin_C. 1 hit.
SUPFAMⁱ	SSF52490. SSF52490. 1 hit. SSF55307. SSF55307. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00227. TUBULIN. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TBA3_YEAST
Accessionⁱ	P09734Primary (citable) accession number: P09734 Secondary accession number(s): D6W0G1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
	Last sequence update:	July 1, 1989
	Last modified:	June 7, 2017
	This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
Yeast chromosome XIII
Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P09734 (TBA3_YEAST)

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Tubulin alpha-3 chain

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ