Reviewed, UniProtKB/Swiss-Prot P09734 (TBA3_YEAST)

Names and origin

Protein names

Recommended name:
    Tubulin alpha-3 chain

Gene names

Name:	TUB3
Ordered Locus Names:	YML124C
ORF Names:	YM7056.02C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Subunit structure	Dimer of alpha and beta chains. Interacts with NUM1. Ref.5
Miscellaneous	Present with 12300 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the tubulin family.

Ontologies

Keywords
Cellular component	Microtubule
Ligand	GTP-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	homologous chromosome segregation Traceable author statement. Source: SGD mitotic sister chromatid segregation Traceable author statement. Source: SGD nuclear migration during conjugation with cellular fusion Traceable author statement. Source: SGD protein polymerization Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasmic microtubule Traceable author statement. Source: SGD kinetochore microtubule Traceable author statement. Source: SGD nuclear microtubule Traceable author statement. Source: SGD polar microtubule Traceable author statement. Source: SGD spindle pole body Inferred from direct assay. Source: SGD
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro protein binding Ref.5 Inferred from physical interaction. Source: IntAct structural constituent of cytoskeleton Traceable author statement. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NUM1	Q00402	1	EBI-18981,EBI-12386

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	Tubulin alpha-3 chain		PRO_0000048240
Regions
	Nucleotide binding	143 – 149	7	GTP Potential

Sequences

	Sequence		Length	Mass (Da)
	P09734-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 189889B795E72448	FASTA	445	49,694
	10 20 30 40 50 60 MREVISINVG QAGCQIGNAC WELYSLEHGI KEDGHLEDGL SKPKGGEEGF STFFHETGYG 70 80 90 100 110 120 KFVPRAIYVD LEPNVIDEVR TGRFKELFHP EQLINGKEDA ANNYARGHYT VGREIVDEVE 130 140 150 160 170 180 ERIRKMADQC DGLQGFLFTH SLGGGTGSGL GSLLLENLSY EYGKKSKLEF AVYPAPQLST 190 200 210 220 230 240 SVVEPYNTVL TTHTTLEHAD CTFMVDNEAI YDICKRNLGI SRPSFSNLNG LIAQVISSVT 250 260 270 280 290 300 ASLRFDGSLN VDLNEFQTNL VPYPRIHFPL VSYAPILSKK RATHESNSVS EITNACFEPG 310 320 330 340 350 360 NQMVKCDPTK GKYMANCLLY RGDVVTRDVQ RAVEQVKNKK TVQMVDWCPT GFKIGICYEP 370 380 390 400 410 420 PSVIPSSELA NVDRAVCMLS NTTAIADAWK RIDQKFDLMY AKRAFVHWYV GEGMEEGEFT 430 440 EAREDLAALE RDYIEVGADS YAEEF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two functional alpha-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins." Schatz P.J., Pillus L., Grisafi P., Solomon F., Botstein D. Mol. Cell. Biol. 6:3711-3721(1986) [PubMed: 3025610] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter." Bun-Ya M., Nishimura M., Harashima S., Oshima Y. Mol. Cell. Biol. 11:3229-3238(1991) [PubMed: 2038328] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-170.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Cortical Num1p interacts with the dynein intermediate chain Pac11p and cytoplasmic microtubules in budding yeast." Farkasovsky M., Kuentzel H. J. Cell Biol. 152:251-262(2001) [PubMed: 11266443] [Abstract] Cited for: INTERACTION WITH NUM1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M28428 mRNA. Translation: AAA35181.1. Z49218 Genomic DNA. Translation: CAA89156.1. D90346 Genomic DNA. No translation available.
PIR	B25076.
RefSeq	NP_013582.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2206N.
IntAct	P09734. 69 interactions.
STRING	P09734.
Proteomic databases
PeptideAtlas	P09734.
PRIDE	P09734.
Genome annotation databases
Ensembl	YML124C; YML124C; YML124C; Saccharomyces cerevisiae. [Genome view]
GeneID	854915.
KEGG	sce:YML124C.
NMPDR	fig|4932.3.peg.4618.
Organism-specific databases
CYGD	YML124c.
SGD	S000004593. TUB3.
Phylogenomic databases
HOGENOM	P09734.
OMA	KRATHES
OrthoDB	EOG9GXH59
Gene expression databases
ArrayExpress	P09734.
Genevestigator	P09734.
GermOnline	YML124C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002452. Alpha_tubulin. IPR008280. Tub_FtsZ_C. IPR000217. Tubulin. IPR018316. Tubulin/FtsZ_2-layer-sand-dom. IPR017975. Tubulin_CS. IPR003008. Tubulin_FtsZ_GTPase. IPR019746. Tubulin_FtsZ_N. [Graphical view]
Gene3D	G3DSA:3.30.1330.20. Tubulin/FtsZ_2-layer-sand-dom. 1 hit. G3DSA:3.40.50.1440. Tubulin_FtsZ. 1 hit.
PANTHER	PTHR11588:SF10. Alpha_tubulin. 1 hit. PTHR11588. Tubulin. 1 hit.
Pfam	PF00091. Tubulin. 1 hit. PF03953. Tubulin_C. 1 hit. [Graphical view]
PRINTS	PR01162. ALPHATUBULIN. PR01161. TUBULIN.
SMART	SM00865. Tubulin_C. 1 hit. [Graphical view]
PROSITE	PS00227. TUBULIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	977920.

Entry information

Entry name

TBA3_YEAST

Accession

Primary (citable) accession number: P09734

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	November 24, 2009
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)