Tubulin alpha-3 chain - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P09734 (TBA3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 102. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Tubulin alpha-3 chain

Gene names

Name:	TUB3
Ordered Locus Names:	YML124C
ORF Names:	YM7056.02C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Subunit structure	Dimer of alpha and beta chains. Interacts with NUM1. Ref.6
Subcellular location	Cytoplasm › cytoskeleton.
Miscellaneous	Present with 12300 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the tubulin family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Microtubule
Ligand	GTP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	homologous chromosome segregation Traceable author statement. Source: SGD mitotic sister chromatid segregation Traceable author statement. Source: SGD nuclear migration involved in conjugation with cellular fusion Traceable author statement. Source: SGD protein polymerization Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasmic microtubule Traceable author statement. Source: SGD kinetochore microtubule Traceable author statement. Source: SGD nuclear microtubule Traceable author statement. Source: SGD polar microtubule Traceable author statement. Source: SGD spindle pole body Inferred from direct assay. Source: SGD
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction Ref.6. Source: IntAct structural constituent of cytoskeleton Traceable author statement. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NUM1	Q00402	2	EBI-18981,EBI-12386

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 445

445

Tubulin alpha-3 chain

PRO_0000048240

Regions

Nucleotide binding

143 – 149

GTP Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P09734 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 189889B795E72448
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FASTA

445

49,694

        10         20         30         40         50         60 
MREVISINVG QAGCQIGNAC WELYSLEHGI KEDGHLEDGL SKPKGGEEGF STFFHETGYG 

        70         80         90        100        110        120 
KFVPRAIYVD LEPNVIDEVR TGRFKELFHP EQLINGKEDA ANNYARGHYT VGREIVDEVE 

       130        140        150        160        170        180 
ERIRKMADQC DGLQGFLFTH SLGGGTGSGL GSLLLENLSY EYGKKSKLEF AVYPAPQLST 

       190        200        210        220        230        240 
SVVEPYNTVL TTHTTLEHAD CTFMVDNEAI YDICKRNLGI SRPSFSNLNG LIAQVISSVT 

       250        260        270        280        290        300 
ASLRFDGSLN VDLNEFQTNL VPYPRIHFPL VSYAPILSKK RATHESNSVS EITNACFEPG 

       310        320        330        340        350        360 
NQMVKCDPTK GKYMANCLLY RGDVVTRDVQ RAVEQVKNKK TVQMVDWCPT GFKIGICYEP 

       370        380        390        400        410        420 
PSVIPSSELA NVDRAVCMLS NTTAIADAWK RIDQKFDLMY AKRAFVHWYV GEGMEEGEFT 

       430        440 
EAREDLAALE RDYIEVGADS YAEEF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two functional alpha-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins." Schatz P.J., Pillus L., Grisafi P., Solomon F., Botstein D. Mol. Cell. Biol. 6:3711-3721(1986) [PubMed: 3025610] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter." Bun-Ya M., Nishimura M., Harashima S., Oshima Y. Mol. Cell. Biol. 11:3229-3238(1991) [PubMed: 2038328] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-170.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Cortical Num1p interacts with the dynein intermediate chain Pac11p and cytoplasmic microtubules in budding yeast." Farkasovsky M., Kuentzel H. J. Cell Biol. 152:251-262(2001) [PubMed: 11266443] [Abstract] Cited for: INTERACTION WITH NUM1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M28428 mRNA. Translation: AAA35181.1. Z49218 Genomic DNA. Translation: CAA89156.1. D90346 Genomic DNA. No translation available. BK006946 Genomic DNA. Translation: DAA09775.1.
PIR	B25076.
RefSeq	NP_013582.1. NM_001182487.1.
3D structure databases
ProteinModelPortal	P09734.
SMR	P09734. Positions 1-440.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2206N.
IntAct	P09734. 63 interactions.
MINT	MINT-617389.
STRING	P09734.
Proteomic databases
PeptideAtlas	P09734.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML124C; YML124C; YML124C.
GeneID	854915.
KEGG	sce:YML124C.
NMPDR	fig\|4932.3.peg.4618.
Organism-specific databases
CYGD	YML124c.
SGD	S000004593. TUB3.
Phylogenomic databases
eggNOG	fuNOG05943.
GeneTree	EFGT00050000001868.
HOGENOM	HBG750007.
OMA	KRATHES.
OrthoDB	EOG470XRR.
Gene expression databases
ArrayExpress	P09734.
Genevestigator	P09734.
GermOnline	YML124C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002452. Alpha_tubulin. IPR008280. Tub_FtsZ_C. IPR000217. Tubulin. IPR018316. Tubulin/FtsZ_2-layer-sand-dom. IPR023123. Tubulin_C. IPR017975. Tubulin_CS. IPR003008. Tubulin_FtsZ_GTPase. [Graphical view]
Gene3D	G3DSA:3.30.1330.20. Tubulin/FtsZ_2-layer-sand-dom. 1 hit. G3DSA:1.10.287.600. Tubulin_C. 1 hit. G3DSA:3.40.50.1440. Tubulin_FtsZ. 1 hit.
PANTHER	PTHR11588. Tubulin. 1 hit.
Pfam	PF00091. Tubulin. 1 hit. PF03953. Tubulin_C. 1 hit. [Graphical view]
PRINTS	PR01162. ALPHATUBULIN. PR01161. TUBULIN.
SMART	SM00864. Tubulin. 1 hit. SM00865. Tubulin_C. 1 hit. [Graphical view]
SUPFAM	SSF55307. Tub_FtsZ_C. 1 hit. SSF52490. Tubulin_FtsZ. 1 hit.
PROSITE	PS00227. TUBULIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977920.

Entry information

Entry name

TBA3_YEAST

Accession

Primary (citable) accession number: P09734
Secondary accession number(s): D6W0G1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	December 14, 2011
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents