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Protein

Tubulin alpha-3 chain

Gene

TUB3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi143 – 1497GTPSequence Analysis

GO - Molecular functioni

  1. GTPase activity Source: InterPro
  2. GTP binding Source: UniProtKB-KW
  3. structural constituent of cytoskeleton Source: InterPro

GO - Biological processi

  1. homologous chromosome segregation Source: SGD
  2. mitotic sister chromatid segregation Source: SGD
  3. nuclear migration along microtubule Source: SGD
  4. protein polymerization Source: InterPro
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32703-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tubulin alpha-3 chain
Gene namesi
Name:TUB3
Ordered Locus Names:YML124C
ORF Names:YM7056.02C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

CYGDiYML124c.
EuPathDBiFungiDB:YML124C.
SGDiS000004593. TUB3.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasmic microtubule Source: SGD
  2. kinetochore microtubule Source: SGD
  3. nuclear microtubule Source: SGD
  4. spindle pole body Source: SGD
  5. tubulin complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 445445Tubulin alpha-3 chainPRO_0000048240Add
BLAST

Proteomic databases

MaxQBiP09734.
PaxDbiP09734.
PeptideAtlasiP09734.
PRIDEiP09734.

Expressioni

Gene expression databases

GenevestigatoriP09734.

Interactioni

Subunit structurei

Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Interacts with NUM1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NUM1Q004022EBI-18981,EBI-12386

Protein-protein interaction databases

BioGridi35081. 162 interactions.
DIPiDIP-2206N.
IntActiP09734. 63 interactions.
MINTiMINT-617389.
STRINGi4932.YML124C.

Structurei

3D structure databases

ProteinModelPortaliP09734.
SMRiP09734. Positions 1-439.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tubulin family.Curated

Phylogenomic databases

eggNOGiCOG5023.
GeneTreeiENSGT00780000122166.
HOGENOMiHOG000165711.
InParanoidiP09734.
OMAiCDSKREC.
OrthoDBiEOG7PVWZW.

Family and domain databases

Gene3Di1.10.287.600. 1 hit.
3.30.1330.20. 1 hit.
3.40.50.1440. 1 hit.
InterProiIPR002452. Alpha_tubulin.
IPR008280. Tub_FtsZ_C.
IPR000217. Tubulin.
IPR018316. Tubulin/FtsZ_2-layer-sand-dom.
IPR023123. Tubulin_C.
IPR017975. Tubulin_CS.
IPR003008. Tubulin_FtsZ_GTPase.
[Graphical view]
PANTHERiPTHR11588. PTHR11588. 1 hit.
PfamiPF00091. Tubulin. 1 hit.
PF03953. Tubulin_C. 1 hit.
[Graphical view]
PRINTSiPR01162. ALPHATUBULIN.
PR01161. TUBULIN.
SMARTiSM00864. Tubulin. 1 hit.
SM00865. Tubulin_C. 1 hit.
[Graphical view]
SUPFAMiSSF52490. SSF52490. 1 hit.
SSF55307. SSF55307. 1 hit.
PROSITEiPS00227. TUBULIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09734-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREVISINVG QAGCQIGNAC WELYSLEHGI KEDGHLEDGL SKPKGGEEGF
60 70 80 90 100
STFFHETGYG KFVPRAIYVD LEPNVIDEVR TGRFKELFHP EQLINGKEDA
110 120 130 140 150
ANNYARGHYT VGREIVDEVE ERIRKMADQC DGLQGFLFTH SLGGGTGSGL
160 170 180 190 200
GSLLLENLSY EYGKKSKLEF AVYPAPQLST SVVEPYNTVL TTHTTLEHAD
210 220 230 240 250
CTFMVDNEAI YDICKRNLGI SRPSFSNLNG LIAQVISSVT ASLRFDGSLN
260 270 280 290 300
VDLNEFQTNL VPYPRIHFPL VSYAPILSKK RATHESNSVS EITNACFEPG
310 320 330 340 350
NQMVKCDPTK GKYMANCLLY RGDVVTRDVQ RAVEQVKNKK TVQMVDWCPT
360 370 380 390 400
GFKIGICYEP PSVIPSSELA NVDRAVCMLS NTTAIADAWK RIDQKFDLMY
410 420 430 440
AKRAFVHWYV GEGMEEGEFT EAREDLAALE RDYIEVGADS YAEEF
Length:445
Mass (Da):49,694
Last modified:July 1, 1989 - v1
Checksum:i189889B795E72448
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28428 mRNA. Translation: AAA35181.1.
Z49218 Genomic DNA. Translation: CAA89156.1.
D90346 Genomic DNA. No translation available.
BK006946 Genomic DNA. Translation: DAA09775.1.
PIRiB25076.
RefSeqiNP_013582.1. NM_001182487.1.

Genome annotation databases

EnsemblFungiiYML124C; YML124C; YML124C.
GeneIDi854915.
KEGGisce:YML124C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28428 mRNA. Translation: AAA35181.1.
Z49218 Genomic DNA. Translation: CAA89156.1.
D90346 Genomic DNA. No translation available.
BK006946 Genomic DNA. Translation: DAA09775.1.
PIRiB25076.
RefSeqiNP_013582.1. NM_001182487.1.

3D structure databases

ProteinModelPortaliP09734.
SMRiP09734. Positions 1-439.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35081. 162 interactions.
DIPiDIP-2206N.
IntActiP09734. 63 interactions.
MINTiMINT-617389.
STRINGi4932.YML124C.

Proteomic databases

MaxQBiP09734.
PaxDbiP09734.
PeptideAtlasiP09734.
PRIDEiP09734.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML124C; YML124C; YML124C.
GeneIDi854915.
KEGGisce:YML124C.

Organism-specific databases

CYGDiYML124c.
EuPathDBiFungiDB:YML124C.
SGDiS000004593. TUB3.

Phylogenomic databases

eggNOGiCOG5023.
GeneTreeiENSGT00780000122166.
HOGENOMiHOG000165711.
InParanoidiP09734.
OMAiCDSKREC.
OrthoDBiEOG7PVWZW.

Enzyme and pathway databases

BioCyciYEAST:G3O-32703-MONOMER.

Miscellaneous databases

NextBioi977920.
PROiP09734.

Gene expression databases

GenevestigatoriP09734.

Family and domain databases

Gene3Di1.10.287.600. 1 hit.
3.30.1330.20. 1 hit.
3.40.50.1440. 1 hit.
InterProiIPR002452. Alpha_tubulin.
IPR008280. Tub_FtsZ_C.
IPR000217. Tubulin.
IPR018316. Tubulin/FtsZ_2-layer-sand-dom.
IPR023123. Tubulin_C.
IPR017975. Tubulin_CS.
IPR003008. Tubulin_FtsZ_GTPase.
[Graphical view]
PANTHERiPTHR11588. PTHR11588. 1 hit.
PfamiPF00091. Tubulin. 1 hit.
PF03953. Tubulin_C. 1 hit.
[Graphical view]
PRINTSiPR01162. ALPHATUBULIN.
PR01161. TUBULIN.
SMARTiSM00864. Tubulin. 1 hit.
SM00865. Tubulin_C. 1 hit.
[Graphical view]
SUPFAMiSSF52490. SSF52490. 1 hit.
SSF55307. SSF55307. 1 hit.
PROSITEiPS00227. TUBULIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two functional alpha-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins."
    Schatz P.J., Pillus L., Grisafi P., Solomon F., Botstein D.
    Mol. Cell. Biol. 6:3711-3721(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter."
    Bun-Ya M., Nishimura M., Harashima S., Oshima Y.
    Mol. Cell. Biol. 11:3229-3238(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-170.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Cortical Num1p interacts with the dynein intermediate chain Pac11p and cytoplasmic microtubules in budding yeast."
    Farkasovsky M., Kuentzel H.
    J. Cell Biol. 152:251-262(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NUM1.

Entry informationi

Entry nameiTBA3_YEAST
AccessioniPrimary (citable) accession number: P09734
Secondary accession number(s): D6W0G1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 29, 2015
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 12300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.