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P09734

- TBA3_YEAST

UniProt

P09734 - TBA3_YEAST

Protein

Tubulin alpha-3 chain

Gene

TUB3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi143 – 1497GTPSequence Analysis

    GO - Molecular functioni

    1. GTPase activity Source: InterPro
    2. GTP binding Source: UniProtKB-KW
    3. protein binding Source: IntAct
    4. structural constituent of cytoskeleton Source: InterPro

    GO - Biological processi

    1. homologous chromosome segregation Source: SGD
    2. mitotic sister chromatid segregation Source: SGD
    3. nuclear migration along microtubule Source: SGD
    4. protein polymerization Source: InterPro

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32703-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tubulin alpha-3 chain
    Gene namesi
    Name:TUB3
    Ordered Locus Names:YML124C
    ORF Names:YM7056.02C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYML124c.
    SGDiS000004593. TUB3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasmic microtubule Source: SGD
    2. kinetochore microtubule Source: SGD
    3. nuclear microtubule Source: SGD
    4. spindle pole body Source: SGD
    5. tubulin complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 445445Tubulin alpha-3 chainPRO_0000048240Add
    BLAST

    Proteomic databases

    MaxQBiP09734.
    PaxDbiP09734.
    PeptideAtlasiP09734.
    PRIDEiP09734.

    Expressioni

    Gene expression databases

    GenevestigatoriP09734.

    Interactioni

    Subunit structurei

    Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Interacts with NUM1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    NUM1Q004022EBI-18981,EBI-12386

    Protein-protein interaction databases

    BioGridi35081. 160 interactions.
    DIPiDIP-2206N.
    IntActiP09734. 63 interactions.
    MINTiMINT-617389.
    STRINGi4932.YML124C.

    Structurei

    3D structure databases

    ProteinModelPortaliP09734.
    SMRiP09734. Positions 1-439.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tubulin family.Curated

    Phylogenomic databases

    eggNOGiCOG5023.
    GeneTreeiENSGT00750000117755.
    HOGENOMiHOG000165711.
    OMAiTRATHEA.
    OrthoDBiEOG7PVWZW.

    Family and domain databases

    Gene3Di1.10.287.600. 1 hit.
    3.30.1330.20. 1 hit.
    3.40.50.1440. 1 hit.
    InterProiIPR002452. Alpha_tubulin.
    IPR008280. Tub_FtsZ_C.
    IPR000217. Tubulin.
    IPR018316. Tubulin/FtsZ_2-layer-sand-dom.
    IPR023123. Tubulin_C.
    IPR017975. Tubulin_CS.
    IPR003008. Tubulin_FtsZ_GTPase.
    [Graphical view]
    PANTHERiPTHR11588. PTHR11588. 1 hit.
    PfamiPF00091. Tubulin. 1 hit.
    PF03953. Tubulin_C. 1 hit.
    [Graphical view]
    PRINTSiPR01162. ALPHATUBULIN.
    PR01161. TUBULIN.
    SMARTiSM00864. Tubulin. 1 hit.
    SM00865. Tubulin_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF52490. SSF52490. 1 hit.
    SSF55307. SSF55307. 1 hit.
    PROSITEiPS00227. TUBULIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P09734-1 [UniParc]FASTAAdd to Basket

    « Hide

    MREVISINVG QAGCQIGNAC WELYSLEHGI KEDGHLEDGL SKPKGGEEGF    50
    STFFHETGYG KFVPRAIYVD LEPNVIDEVR TGRFKELFHP EQLINGKEDA 100
    ANNYARGHYT VGREIVDEVE ERIRKMADQC DGLQGFLFTH SLGGGTGSGL 150
    GSLLLENLSY EYGKKSKLEF AVYPAPQLST SVVEPYNTVL TTHTTLEHAD 200
    CTFMVDNEAI YDICKRNLGI SRPSFSNLNG LIAQVISSVT ASLRFDGSLN 250
    VDLNEFQTNL VPYPRIHFPL VSYAPILSKK RATHESNSVS EITNACFEPG 300
    NQMVKCDPTK GKYMANCLLY RGDVVTRDVQ RAVEQVKNKK TVQMVDWCPT 350
    GFKIGICYEP PSVIPSSELA NVDRAVCMLS NTTAIADAWK RIDQKFDLMY 400
    AKRAFVHWYV GEGMEEGEFT EAREDLAALE RDYIEVGADS YAEEF 445
    Length:445
    Mass (Da):49,694
    Last modified:July 1, 1989 - v1
    Checksum:i189889B795E72448
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M28428 mRNA. Translation: AAA35181.1.
    Z49218 Genomic DNA. Translation: CAA89156.1.
    D90346 Genomic DNA. No translation available.
    BK006946 Genomic DNA. Translation: DAA09775.1.
    PIRiB25076.
    RefSeqiNP_013582.1. NM_001182487.1.

    Genome annotation databases

    EnsemblFungiiYML124C; YML124C; YML124C.
    GeneIDi854915.
    KEGGisce:YML124C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M28428 mRNA. Translation: AAA35181.1 .
    Z49218 Genomic DNA. Translation: CAA89156.1 .
    D90346 Genomic DNA. No translation available.
    BK006946 Genomic DNA. Translation: DAA09775.1 .
    PIRi B25076.
    RefSeqi NP_013582.1. NM_001182487.1.

    3D structure databases

    ProteinModelPortali P09734.
    SMRi P09734. Positions 1-439.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35081. 160 interactions.
    DIPi DIP-2206N.
    IntActi P09734. 63 interactions.
    MINTi MINT-617389.
    STRINGi 4932.YML124C.

    Proteomic databases

    MaxQBi P09734.
    PaxDbi P09734.
    PeptideAtlasi P09734.
    PRIDEi P09734.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML124C ; YML124C ; YML124C .
    GeneIDi 854915.
    KEGGi sce:YML124C.

    Organism-specific databases

    CYGDi YML124c.
    SGDi S000004593. TUB3.

    Phylogenomic databases

    eggNOGi COG5023.
    GeneTreei ENSGT00750000117755.
    HOGENOMi HOG000165711.
    OMAi TRATHEA.
    OrthoDBi EOG7PVWZW.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32703-MONOMER.

    Miscellaneous databases

    NextBioi 977920.

    Gene expression databases

    Genevestigatori P09734.

    Family and domain databases

    Gene3Di 1.10.287.600. 1 hit.
    3.30.1330.20. 1 hit.
    3.40.50.1440. 1 hit.
    InterProi IPR002452. Alpha_tubulin.
    IPR008280. Tub_FtsZ_C.
    IPR000217. Tubulin.
    IPR018316. Tubulin/FtsZ_2-layer-sand-dom.
    IPR023123. Tubulin_C.
    IPR017975. Tubulin_CS.
    IPR003008. Tubulin_FtsZ_GTPase.
    [Graphical view ]
    PANTHERi PTHR11588. PTHR11588. 1 hit.
    Pfami PF00091. Tubulin. 1 hit.
    PF03953. Tubulin_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01162. ALPHATUBULIN.
    PR01161. TUBULIN.
    SMARTi SM00864. Tubulin. 1 hit.
    SM00865. Tubulin_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52490. SSF52490. 1 hit.
    SSF55307. SSF55307. 1 hit.
    PROSITEi PS00227. TUBULIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two functional alpha-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins."
      Schatz P.J., Pillus L., Grisafi P., Solomon F., Botstein D.
      Mol. Cell. Biol. 6:3711-3721(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter."
      Bun-Ya M., Nishimura M., Harashima S., Oshima Y.
      Mol. Cell. Biol. 11:3229-3238(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-170.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Cortical Num1p interacts with the dynein intermediate chain Pac11p and cytoplasmic microtubules in budding yeast."
      Farkasovsky M., Kuentzel H.
      J. Cell Biol. 152:251-262(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NUM1.

    Entry informationi

    Entry nameiTBA3_YEAST
    AccessioniPrimary (citable) accession number: P09734
    Secondary accession number(s): D6W0G1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 12300 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3