Reviewed, UniProtKB/Swiss-Prot P09733 (TBA1_YEAST)

Names and origin

Protein names

Recommended name:
    Tubulin alpha-1 chain

Gene names

Name:	TUB1
Ordered Locus Names:	YML085C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Subunit structure	Dimer of alpha and beta chains.
Miscellaneous	Present with 5590 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the tubulin family.

Ontologies

Keywords
Cellular component	Microtubule
Ligand	GTP-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	homologous chromosome segregation Traceable author statement. Source: SGD mitotic sister chromatid segregation Traceable author statement. Source: SGD nuclear migration during conjugation with cellular fusion Traceable author statement. Source: SGD protein polymerization Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasmic microtubule Traceable author statement. Source: SGD kinetochore microtubule Traceable author statement. Source: SGD nuclear microtubule Traceable author statement. Source: SGD polar microtubule Traceable author statement. Source: SGD spindle pole body Inferred from direct assay. Source: SGD
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct structural constituent of cytoskeleton Traceable author statement. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q12486	1	EBI-18976,EBI-30442

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	Tubulin alpha-1 chain		PRO_0000048239
Regions
	Nucleotide binding	143 – 149	7	GTP Potential
Amino acid modifications
	Modified residue	274	1	Phosphoserine Ref.4
Experimental info
	Sequence conflict	94	1	I → L in AAA35180. Ref.1
	Sequence conflict	327	1	R → G in AAA35180. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P09733-1 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: E263B49440F71889	FASTA	447	49,800
	10 20 30 40 50 60 MREVISINVG QAGCQIGNAC WELYSLEHGI KPDGHLEDGL SKPKGGEEGF STFFHETGYG 70 80 90 100 110 120 KFVPRAIYVD LEPNVIDEVR NGPYKDLFHP EQLISGKEDA ANNYARGHYT VGREILGDVL 130 140 150 160 170 180 DRIRKLADQC DGLQGFLFTH SLGGGTGSGL GSLLLEELSA EYGKKSKLEF AVYPAPQVST 190 200 210 220 230 240 SVVEPYNTVL TTHTTLEHAD CTFMVDNEAI YDMCKRNLDI PRPSFANLNN LIAQVVSSVT 250 260 270 280 290 300 ASLRFDGSLN VDLNEFQTNL VPYPRIHFPL VSYSPVLSKS KAFHESNSVS EITNACFEPG 310 320 330 340 350 360 NQMVKCDPRD GKYMATCLLY RGDVVTRDVQ RAVEQVKNKK TVQLVDWCPT GFKIGICYEP 370 380 390 400 410 420 PTATPNSQLA TVDRAVCMLS NTTSIAEAWK RIDRKFDLMY AKRAFVHWYV GEGMEEGEFT 430 440 EAREDLAALE RDYIEVGADS YAEEEEF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two functional alpha-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins." Schatz P.J., Pillus L., Grisafi P., Solomon F., Botstein D. Mol. Cell. Biol. 6:3711-3721(1986) [PubMed: 3025610] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-274, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M28429 mRNA. Translation: AAA35180.1. Z46660 Genomic DNA. Translation: CAA86653.1.
PIR	S50871.
RefSeq	NP_013625.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:854N.
IntAct	P09733. 258 interactions.
STRING	P09733.
Proteomic databases
PeptideAtlas	P09733.
PRIDE	P09733.
Genome annotation databases
Ensembl	YML085C; YML085C; YML085C; Saccharomyces cerevisiae. [Genome view]
GeneID	854889.
KEGG	sce:YML085C.
NMPDR	fig|4932.3.peg.4662.
Organism-specific databases
CYGD	YML085c.
SGD	S000004550. TUB1.
Phylogenomic databases
HOGENOM	P09733.
OMA	ANHEANS
OrthoDB	EOG9DJMCS
Gene expression databases
ArrayExpress	P09733.
Genevestigator	P09733.
GermOnline	YML085C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002452. Alpha_tubulin. IPR008280. Tub_FtsZ_C. IPR000217. Tubulin. IPR018316. Tubulin/FtsZ_2-layer-sand-dom. IPR017975. Tubulin_CS. IPR003008. Tubulin_FtsZ_GTPase. IPR019746. Tubulin_FtsZ_N. [Graphical view]
Gene3D	G3DSA:3.30.1330.20. Tubulin/FtsZ_2-layer-sand-dom. 1 hit. G3DSA:3.40.50.1440. Tubulin_FtsZ. 1 hit.
PANTHER	PTHR11588:SF10. Alpha_tubulin. 1 hit. PTHR11588. Tubulin. 1 hit.
Pfam	PF00091. Tubulin. 1 hit. PF03953. Tubulin_C. 1 hit. [Graphical view]
PRINTS	PR01162. ALPHATUBULIN. PR01161. TUBULIN.
SMART	SM00865. Tubulin_C. 1 hit. [Graphical view]
PROSITE	PS00227. TUBULIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	977846.

Entry information

Entry name

TBA1_YEAST

Accession

Primary (citable) accession number: P09733

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	October 1, 1996
Last modified:	November 24, 2009
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)